ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    z theta ecis system  (Applied BioPhysics)


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    Applied BioPhysics z theta ecis system
    Z Theta Ecis System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta device  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta device
    Ecis Z Theta Device, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta station  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta station
    Ecis Z Theta Station, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta instrument
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Group A Streptococcus strains causing meningitis without distinct invasive phenotype"

    Article Title: Group A Streptococcus strains causing meningitis without distinct invasive phenotype

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.1394

    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Figure Legend Snippet: Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Techniques Used: Infection, Disruption, Lysis, Activity Assay, Generated, Bacteria, Standard Deviation, MANN-WHITNEY

    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis z theta instrument
    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® <t>Z‐Theta</t> instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.
    Ecis Z Theta Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Journal: MicrobiologyOpen

    Article Title: Group A Streptococcus strains causing meningitis without distinct invasive phenotype

    doi: 10.1002/mbo3.1394

    Figure Lengend Snippet: Bacterial strains virulence determinants. (a) Adherence to and (b) invasion of human brain vascular endothelial cells (HBMECs) were assessed after 30 min (MOI 1) or 2 h (MOI 10) of infection, respectively. Adherence and invasion percentages were calculated based on the initial bacterial inoculum used to infect HBMECs. Bacterial adherence above 100% occurred in one case and could be explained by increased initial bacterial growth or by disruption of long bacterial chains or clumps due to sheer pipetting forces during the eukaryotic cell lysis step. Bacterial virulence factors activity of (c) Streptococcal DNases, (d) the IL‐8 protease SpyCEP, and (e) the pore‐forming toxin SLO were assessed in supernatants of exponentially growing GAS. (f) HBMECs barrier function disruption was carried out with the ECIS® Z‐Theta instrument (Applied Biophysics), measuring the impedance generated by the cell barrier in the presence or absence of bacteria. Control experiments were carried out using medium only. (a–d) The average of the biological replicates for each strain is depicted as a single data point on the graphs so that each data point represents a single strain. The two strains indicated in the figure legend with a black symbol (CI407 and CI571) are the only strains of emm ‐type 28 (M28), as opposed to all other strains that are emm ‐type 1 (M1). (a–e) Three biological replicates were carried out for each strain. The average values for strains from a single group of isolates (meningitis, otitis, or colonizing) are depicted in the graphs and the error bars represent the standard deviation. (f) Two biological replicates were carried out for each strain and the average of the strains from to the same group is depicted on the graph. Control = medium only. No significant differences in adherence, invasion, virulence factors activity, or barrier disruption were observed among the different groups of isolates. A significant difference was found between the respective controls and meningitis, otitis, or colonizing isolates for DNase ( p = 0.0159, 0.0159, and 0.0159, respectively) and SpyCEP ( p = 0.0179, 0.0357, and 0.0179, respectively) activity. Statistical significance was assessed using the Mann–Whitney test. Col, colonizing; Ctrl, control; Men, meningitis.

    Article Snippet: ECIS® was performed with the ECIS® Z‐Theta instrument (Applied Biophysics) to monitor the effects of the GAS strains on human brain microvascular endothelial cells (HBMECs) (Nizet et al., ) barrier integrity.

    Techniques: Infection, Disruption, Lysis, Activity Assay, Generated, Bacteria, Standard Deviation, MANN-WHITNEY