ecis model ztheta  (Applied BioPhysics)


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    Applied BioPhysics ecis model ztheta
    Ecis Model Ztheta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    ecis model ztheta - by Bioz Stars, 2023-01
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis z theta system/product/Applied BioPhysics
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    ecis model ztheta  (Applied BioPhysics)


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    Applied BioPhysics ecis model ztheta
    Ecis Model Ztheta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z θ instrument  (Applied BioPhysics)


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    Applied BioPhysics ecis z θ instrument
    Ecis Z θ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis z θ instrument/product/Applied BioPhysics
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    instrument ecis zθ theta  (Applied BioPhysics)


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    Applied BioPhysics instrument ecis zθ theta
    Instrument Ecis Zθ Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecis z theta  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta
    Ecis Z Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis z theta/product/Applied BioPhysics
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    ecis ztheta apparatus  (Applied BioPhysics)


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    Applied BioPhysics ecis ztheta apparatus
    Ecis Ztheta Apparatus, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis ztheta apparatus/product/Applied BioPhysics
    Average 95 stars, based on 1 article reviews
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    ecis ztheta apparatus  (Applied BioPhysics)


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    Applied BioPhysics ecis ztheta apparatus
    Ecis Ztheta Apparatus, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis ztheta apparatus/product/Applied BioPhysics
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    z theta  (Applied BioPhysics)


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    Applied BioPhysics z theta
    HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on <t>96-well</t> <t>electrodes</t> and electrical resistance was measured using the <t>Z-Theta</t> (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)
    Z Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z theta/product/Applied BioPhysics
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    1) Product Images from "Extracellular microvesicles released from brain endothelial cells are detected in animal models of HIV-1 signifying unresolved inflammation"

    Article Title: Extracellular microvesicles released from brain endothelial cells are detected in animal models of HIV-1 signifying unresolved inflammation

    Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

    doi: 10.1007/s11481-021-10008-5

    HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on 96-well electrodes and electrical resistance was measured using the Z-Theta (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)
    Figure Legend Snippet: HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on 96-well electrodes and electrical resistance was measured using the Z-Theta (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)

    Techniques Used: Permeability, Isolation, Labeling, Flow Cytometry

    ecis z theta system  (Applied BioPhysics)


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    Applied BioPhysics ecis z theta system
    Ecis Z Theta System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis z theta system/product/Applied BioPhysics
    Average 95 stars, based on 1 article reviews
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    ecis z theta system - by Bioz Stars, 2023-01
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    Applied BioPhysics ecis model ztheta
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    Applied BioPhysics instrument ecis zθ theta
    Instrument Ecis Zθ Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis z theta
    Ecis Z Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics ecis ztheta apparatus
    Ecis Ztheta Apparatus, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecis ztheta apparatus/product/Applied BioPhysics
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    Applied BioPhysics z theta
    HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on <t>96-well</t> <t>electrodes</t> and electrical resistance was measured using the <t>Z-Theta</t> (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)
    Z Theta, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z theta/product/Applied BioPhysics
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    z theta - by Bioz Stars, 2023-01
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    HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on 96-well electrodes and electrical resistance was measured using the Z-Theta (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)

    Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

    Article Title: Extracellular microvesicles released from brain endothelial cells are detected in animal models of HIV-1 signifying unresolved inflammation

    doi: 10.1007/s11481-021-10008-5

    Figure Lengend Snippet: HIV viroproteins and inflammatory insults disrupt the primary human brain microvasculature endothelial barrier and corresponds to an increase in microvesicles containing the tight junction protein occludin. A. hBMVECs were grown on 96-well electrodes and electrical resistance was measured using the Z-Theta (Applied Biophysics). Cells were stimulated with 100 ng/mL of HIV-1 proteins Tat and gp120 (A) or 100 ng/mL of inflammatory insults TNF-α and LPS (B). All insults resulted in a reduction in electrical resistance equating to an increase in permeability in the endothelial monolayer. Similarly, hBMVECs grown in 100 mm dishes were treated with 100 ng/ml of LPS (B) TNF-α (C), Tat (D) and gp120 (E) for 6 h and 24 h. Media was harvested, MVs were isolated by ultracentrifugation, labeled with Occludin-FITC and analyzed by flow cytometry. Data was normalized by the basal production over the same treatment time from the same cells (paired study). (n = 7–18 all conditions, students t-test, *p < 0.5 **p < 0.01)

    Article Snippet: A. hBMVECs were grown on 96-well electrodes and electrical resistance was measured using the Z-Theta (Applied Biophysics).

    Techniques: Permeability, Isolation, Labeling, Flow Cytometry