amylose resin high flow  (New England Biolabs)


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    Name:
    Amylose Resin High Flow
    Description:
    Amylose Resin High Flow 100 ml
    Catalog Number:
    E8022L
    Price:
    1730
    Category:
    Protein Purification Kit Components
    Size:
    100 ml
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    Structured Review

    New England Biolabs amylose resin high flow
    Amylose Resin High Flow
    Amylose Resin High Flow 100 ml
    https://www.bioz.com/result/amylose resin high flow/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amylose resin high flow - by Bioz Stars, 2021-05
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein
    Article Snippet: .. Clear lysates were then incubated with 800 μL of amylose fast flow resin beads (New England Biolabs) overnight at 4° C, allowing for optimal binding of MBP to the resin beads. .. Following overnight incubation, beads were spun down and supernatant was removed, after which beads were resuspended in amylose column buffer.

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Article Title: Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein
    Article Snippet: .. After centrifugation, the bacteria were resuspended to PBS with 5 mM EDTA and lysed by sonication for 10 min. After centrifugation, the supernatants were incubated with affinity resin column: GST accept resin (Nacalai Tesque) for GST-fused proteins and Amylose Resin High Flow resin for MBP-fused proteins (New England Biolabs). .. After washing the resin with PBS three times, the proteins were eluted with glutathione buffer (10 mM glutathione and 50 mM Tris-HCl pH 8.0) for GST-fused proteins or maltose buffer (10 mM maltose, 20 mM Tris-HCl pH 8.0, 200 mM sodium chloride) for MBP-fused proteins.

    Flow Cytometry:

    Article Title: Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein
    Article Snippet: .. Clear lysates were then incubated with 800 μL of amylose fast flow resin beads (New England Biolabs) overnight at 4° C, allowing for optimal binding of MBP to the resin beads. .. Following overnight incubation, beads were spun down and supernatant was removed, after which beads were resuspended in amylose column buffer.

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Article Title: Tryptophanyl-tRNA Synthetase Urzyme
    Article Snippet: .. The supernatant from this step was diluted 1:5 with the amylose resin High Flow (New England Biolabs) column buffer (20 m m Tris-HCl, 0.2 m NaCl, 1 m m EDTA, 10 m m β-mercaptoethanol, pH 7.4) before loading onto a disposable column (Bio-Rad) containing 2 ml of amylose-conjugated resin suspension for each 10 ml of the diluted supernatant. ..

    Article Title: Structure and non-essential function of glycerol kinase in Plasmodium falciparum blood stages
    Article Snippet: The cells were harvested by centrifugation and resuspended in buffer A (50 mM Tris pH 8.0 containing 50 mM arginine and 50 mM glutamate). .. Cell extract was loaded onto amylose resin high flow (New England Biolabs), equilibrated with buffer A , and the fusion protein was eluted in buffer A containing 10 mM maltose. .. Fractions containing PfGK–MBP were pooled for anion exchange chromatography using Q-Sepharose.

    Article Title: Phototropin perceives temperature based on the lifetime of its photoactivated state
    Article Snippet: After centrifugation at 18,000 × g for 20 min at 4 °C, the bacterial cells were frozen at −20 °C, thawed on ice, and resuspended in lysis buffer [50 mM Tris HCl (pH 8.0), 150 mM NaCl, and 2 mM CaCl2 ] with 200 µg of ml−1 lysozyme and 0.2% Triton X-100. .. The cells were lysed by sonication on ice and the MBP-tagged recombinant proteins were purified using Amylose Resin High Flow (New England Biolabs) and Poly-Prep Chromatography Columns (Bio-Rad) according to the manufacturers’ protocols. .. The MBP–LOV proteins were concentrated with Amicon Ultra Centrifucal Filters (Millipore) and diluted in 20 mM Tris⋅HCl (pH 7.8) buffer containing 200 mM NaCl and 10% glycerol.

    Article Title: Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein
    Article Snippet: .. After centrifugation, the bacteria were resuspended to PBS with 5 mM EDTA and lysed by sonication for 10 min. After centrifugation, the supernatants were incubated with affinity resin column: GST accept resin (Nacalai Tesque) for GST-fused proteins and Amylose Resin High Flow resin for MBP-fused proteins (New England Biolabs). .. After washing the resin with PBS three times, the proteins were eluted with glutathione buffer (10 mM glutathione and 50 mM Tris-HCl pH 8.0) for GST-fused proteins or maltose buffer (10 mM maltose, 20 mM Tris-HCl pH 8.0, 200 mM sodium chloride) for MBP-fused proteins.

    Binding Assay:

    Article Title: Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein
    Article Snippet: .. Clear lysates were then incubated with 800 μL of amylose fast flow resin beads (New England Biolabs) overnight at 4° C, allowing for optimal binding of MBP to the resin beads. .. Following overnight incubation, beads were spun down and supernatant was removed, after which beads were resuspended in amylose column buffer.

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Protein Purification:

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Column Chromatography:

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Chromatography:

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Article Title: Phototropin perceives temperature based on the lifetime of its photoactivated state
    Article Snippet: After centrifugation at 18,000 × g for 20 min at 4 °C, the bacterial cells were frozen at −20 °C, thawed on ice, and resuspended in lysis buffer [50 mM Tris HCl (pH 8.0), 150 mM NaCl, and 2 mM CaCl2 ] with 200 µg of ml−1 lysozyme and 0.2% Triton X-100. .. The cells were lysed by sonication on ice and the MBP-tagged recombinant proteins were purified using Amylose Resin High Flow (New England Biolabs) and Poly-Prep Chromatography Columns (Bio-Rad) according to the manufacturers’ protocols. .. The MBP–LOV proteins were concentrated with Amicon Ultra Centrifucal Filters (Millipore) and diluted in 20 mM Tris⋅HCl (pH 7.8) buffer containing 200 mM NaCl and 10% glycerol.

    Reversed-phase Chromatography:

    Article Title: Cellular mechanism of fibril formation from serum amyloid A1 protein
    Article Snippet: A His‐tag and a tobacco etch virus cleavage site were inserted in between the genes for maltose binding protein and SAA1 to aid in purification. .. Protein purification from the cell lysate was based on column chromatography and consisted of the following steps: (i) chromatography via amylose high flow (New England Biolabs) resin, (ii) chromatography via fast flow nickel–Sepharose (GE Healthcare) medium, (iii) cleavage of the fusion protein with tobacco etch virus protease (overnight incubation at 34°C), (iv) a second nickel chelate chromatography step to separate SAA1 from the His‐tagged maltose binding protein, (v) reversed‐phase chromatography (RPC) with Source 15 RPC (GE Healthcare) medium. .. The purified protein was finally lyophilized with an Alpha 2–4 LD plus freeze dryer (Christ) and stored at −80°C.

    Purification:

    Article Title: EMR, a cytosolic-abundant ring finger E3 ligase, mediates ER-associated protein degradation in Arabidopsis.
    Article Snippet: The plasmids for protein New Phytologist (2018) 220: 163–177 2018 The Authors New Phytologist 2018 New Phytologist Trustwww.newphytologist.com Research New Phytologist164 expression were transformed into Escherichia coli BL21 (DE3). .. MBP-fused or 69His-tagged proteins were purified using Amylose Resin High Flow (New England Biolabs, Ipswich, MA, USA) or Ni2+-charged IMAC-Sepharose 6 Fast Flow (GE Healthcare, South Plainfield, NJ, USA), respectively. .. EMR proteins were further purified using a TSK heparin-5PW HPLC column (7.59 75 mm) as described previously (Kang et al., 2006).

    Article Title: Phototropin perceives temperature based on the lifetime of its photoactivated state
    Article Snippet: After centrifugation at 18,000 × g for 20 min at 4 °C, the bacterial cells were frozen at −20 °C, thawed on ice, and resuspended in lysis buffer [50 mM Tris HCl (pH 8.0), 150 mM NaCl, and 2 mM CaCl2 ] with 200 µg of ml−1 lysozyme and 0.2% Triton X-100. .. The cells were lysed by sonication on ice and the MBP-tagged recombinant proteins were purified using Amylose Resin High Flow (New England Biolabs) and Poly-Prep Chromatography Columns (Bio-Rad) according to the manufacturers’ protocols. .. The MBP–LOV proteins were concentrated with Amicon Ultra Centrifucal Filters (Millipore) and diluted in 20 mM Tris⋅HCl (pH 7.8) buffer containing 200 mM NaCl and 10% glycerol.

    Article Title: PARylation of the forkhead‐associated domain protein DAWDLE regulates plant immunity
    Article Snippet: All data points represent triplicates. .. The MBP‐PARP2 proteins were purified from E. coli strain BL21 with amylose resins (New England Biolabs, USA, E8022L) according to the standard procedure. .. Proteins on microarray chips were incubated with 100 μl of 100 ng/μl MBP‐PARP2 for 1 h at room temperature in 1× PAR reaction buffer [50 mM Tris–HCl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , and 1× activated DNA (Trevigen, USA, 4671‐096‐06)] with or without 0.2 mM NAD+ (Sigma, N0632).

    Sonication:

    Article Title: Phototropin perceives temperature based on the lifetime of its photoactivated state
    Article Snippet: After centrifugation at 18,000 × g for 20 min at 4 °C, the bacterial cells were frozen at −20 °C, thawed on ice, and resuspended in lysis buffer [50 mM Tris HCl (pH 8.0), 150 mM NaCl, and 2 mM CaCl2 ] with 200 µg of ml−1 lysozyme and 0.2% Triton X-100. .. The cells were lysed by sonication on ice and the MBP-tagged recombinant proteins were purified using Amylose Resin High Flow (New England Biolabs) and Poly-Prep Chromatography Columns (Bio-Rad) according to the manufacturers’ protocols. .. The MBP–LOV proteins were concentrated with Amicon Ultra Centrifucal Filters (Millipore) and diluted in 20 mM Tris⋅HCl (pH 7.8) buffer containing 200 mM NaCl and 10% glycerol.

    Article Title: Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein
    Article Snippet: .. After centrifugation, the bacteria were resuspended to PBS with 5 mM EDTA and lysed by sonication for 10 min. After centrifugation, the supernatants were incubated with affinity resin column: GST accept resin (Nacalai Tesque) for GST-fused proteins and Amylose Resin High Flow resin for MBP-fused proteins (New England Biolabs). .. After washing the resin with PBS three times, the proteins were eluted with glutathione buffer (10 mM glutathione and 50 mM Tris-HCl pH 8.0) for GST-fused proteins or maltose buffer (10 mM maltose, 20 mM Tris-HCl pH 8.0, 200 mM sodium chloride) for MBP-fused proteins.

    Recombinant:

    Article Title: Phototropin perceives temperature based on the lifetime of its photoactivated state
    Article Snippet: After centrifugation at 18,000 × g for 20 min at 4 °C, the bacterial cells were frozen at −20 °C, thawed on ice, and resuspended in lysis buffer [50 mM Tris HCl (pH 8.0), 150 mM NaCl, and 2 mM CaCl2 ] with 200 µg of ml−1 lysozyme and 0.2% Triton X-100. .. The cells were lysed by sonication on ice and the MBP-tagged recombinant proteins were purified using Amylose Resin High Flow (New England Biolabs) and Poly-Prep Chromatography Columns (Bio-Rad) according to the manufacturers’ protocols. .. The MBP–LOV proteins were concentrated with Amicon Ultra Centrifucal Filters (Millipore) and diluted in 20 mM Tris⋅HCl (pH 7.8) buffer containing 200 mM NaCl and 10% glycerol.

    Centrifugation:

    Article Title: Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein
    Article Snippet: .. After centrifugation, the bacteria were resuspended to PBS with 5 mM EDTA and lysed by sonication for 10 min. After centrifugation, the supernatants were incubated with affinity resin column: GST accept resin (Nacalai Tesque) for GST-fused proteins and Amylose Resin High Flow resin for MBP-fused proteins (New England Biolabs). .. After washing the resin with PBS three times, the proteins were eluted with glutathione buffer (10 mM glutathione and 50 mM Tris-HCl pH 8.0) for GST-fused proteins or maltose buffer (10 mM maltose, 20 mM Tris-HCl pH 8.0, 200 mM sodium chloride) for MBP-fused proteins.

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  • 95
    New England Biolabs amylose resin high flow
    Amylose Resin High Flow, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amylose resin high flow/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amylose resin high flow - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

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