protocol e7330  (New England Biolabs)


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    Structured Review

    New England Biolabs protocol e7330
    Protocol E7330, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protocol e7330/product/New England Biolabs
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protocol e7330 - by Bioz Stars, 2019-12
    83/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA). .. The PCR products were then purified with the MinElute PCR Purification Kit (Qiagen, Germany) and their length checked on the Bioanalyzer 2100 (Agilent, Germany) using the DNA 1000 Kit (Agilent, Germany).

    Multiplex Assay:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: Library preparation was performed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs Inc., USA). .. This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA).

    RNA Sequencing Assay:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: Paragraph title: Preparation of library for small RNA sequencing ... This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA).

    Construct:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA). .. This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA).

    Purification:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: A total of 200 ng of purified RNA was used as starting material. .. This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA).

    Polymerase Chain Reaction:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA). .. Briefly the steps are: 3′-adaper ligation, followed by reverse transcription primer hybridization (to prevent adaptor-dimer formation), 5′-adapter ligation, a reverse transcription reaction and PCR enrichment (Spornraft et al., ).

    Gel Extraction:

    Article Title: The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
    Article Snippet: This was converted into barcoded cDNA according to Protocol E7330 (New England BioLabs Inc., USA) and sequenced on the Illumina HiSeq2500 platform (Illumina Inc., USA). .. The cDNA constructs were size selected by loading the PCR products on a 4% agarose gel.

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    New England Biolabs protocol e7330
    Protocol E7330, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protocol e7330/product/New England Biolabs
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protocol e7330 - by Bioz Stars, 2019-12
    83/100 stars
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    New England Biolabs small rna library preparation kit
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