epimark bisulfite conversion kit  (New England Biolabs)


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    Name:
    EpiMark Bisulfite Conversion Kit
    Description:
    EpiMark Bisulfite Conversion Kit 48 rxns
    Catalog Number:
    E3318S
    Price:
    162
    Size:
    48 rxns
    Category:
    DNA Fragment Analysis Kits
    Score:
    85
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    Structured Review

    New England Biolabs epimark bisulfite conversion kit
    EpiMark Bisulfite Conversion Kit
    EpiMark Bisulfite Conversion Kit 48 rxns
    https://www.bioz.com/result/epimark bisulfite conversion kit/product/New England Biolabs
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    epimark bisulfite conversion kit - by Bioz Stars, 2019-12
    95/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Paragraph title: Methylation analysis via bisulfite sequencing ... Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions.

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: Paragraph title: Bisulfite sequencing PCR (BSP) for validation of MSAP ... Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.).

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions. .. Detailed information regarding methodology and associated references are available in the .

    Article Title: Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector
    Article Snippet: Paragraph title: Bisulfite Sequencing ... 500 ng genomic DNA was treated with the Epitect Bisulfite Kit (Qiagen) or Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer’s instructions.

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: Genomic DNA was isolated according to standard methods using a proteinase K digest. .. For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. .. Methylation-specific PCR (MSP) and bisulfite sequencing were performed as previously described .

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Paragraph title: 2.3. Bisulfite sequencing ... All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: Paragraph title: Bisulfite sequencing ... Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol.

    Clone Assay:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: CFTR cDNA was synthesized with QuantiTect Reverse Transcription Kit (Qiagen) using 500 ng of total RNA, and used for transcript analysis ( ). .. Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. The CFTR promoter region was amplified using primers specific for the converted sequence (B1/B2, ).

    Article Title: Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector
    Article Snippet: 500 ng genomic DNA was treated with the Epitect Bisulfite Kit (Qiagen) or Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer’s instructions. .. PCR was performed with GoTaq DNA polymerase (Promega).

    Amplification:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. The CFTR promoter region was amplified using primers specific for the converted sequence (B1/B2, ).

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions. .. Detailed information regarding methodology and associated references are available in the .

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB). .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: PCR —1575bp and 1605bp fragments of p38δ MAPK mRNA, corresponding to positions 111–1686 and 82–1686 respectively of NCBI Reference Sequence NM_002754.4 were amplified from cellular cDNA using oligonucleotide primers P003F (5′-CGAGATCGGGTGCCCGGGAT-3′) or P002F (5′-CCGGAAAAAGGGCTTCTACAA-3′) and P001R (5′-CCGCCACAAGCTAAAAAGAG-3′). .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified. .. PCR-products were subjected to Sanger sequencing and methylation status of each CpG site was assessed semi-quantitatively from the sequence chromatograms .

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol. .. Specific genomic regions were amplified using Epimark Taq polymerase (NEB) and primers specific for bisulfite converted DNA (Metabion, Martinsried, Germany).

    Article Title: DNA Methylation of BDNF Gene in Schizophrenia
    Article Snippet: We treated 100 ng DNA samples with EpiMark Bisulfite Conversion Kit (Catalog No. E3318, New England Biolabs), in accordance with the manufacturer’s standard instructions. .. PCR mixture contained 10 ng of modified DNA, 2.0 mmol each of dATP, dGTP, dCTP, and dTTP, 1.0 pmol each of primer, 2.0 mmol MgCl2, 1× reaction buffer, and 1.0 U Taq polymerase.

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA). .. MS-PCR assays were carried out as published in our previous study [ ].

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified. .. PCR-products were subjected to Sanger sequencing and methylation status of each CpG site was assessed semi-quantitatively from the sequence chromatograms .

    Mass Spectrometry:

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: To assess the compatibility of DNA extracted using five prioritized protocols (AllPrep, QIAamp, RecAll, DNeasy, and GenJet) with enzyme-based downstream reactions, we performed methylation-specific PCR (MS-PCR), which is frequently employed in biomarker studies [ ]. .. As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA). .. MS-PCR assays were carried out as published in our previous study [ ].

    Positive Control:

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA). .. Briefly, 2 μL of the purified DNA was used in a 10-μL MS-PCR reaction involving amplification of targets in the bisulfite-converted CpG islands of the genes GSTP1 , ABCB1 and RASSF1 , all known to be hypermethylated in prostate cancer.

    Nucleic Acid Electrophoresis:

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: For physical analysis of inversion duplications, 3 μg of genomic DNA was digested with 20 units of the indicated restriction endonucleases, separated by gel electrophoresis and transferred to Biobond-Plus nylon membrane (Sigma) for hybridization. .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions.

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual. .. The PCR reaction mixture consisted of 4 μl of the bisulfite modified DNA eluate (40 μl), 1X PCR reaction buffer, 0.2 mM dNTP mixture, 0.2 μM each forward and reverse primer, and 1 unit of TaKaRa Taq HS (Takara Bio Inc., Japan) in a volume of 25 μl.

    Quantitative RT-PCR:

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Quantitative rt-PCR —p38δ MAPK mRNA expression was quantitated using a Qiagen QuantiFast® MAPK13 Probe Assay with one-step rt-PCR and simultaneous detection of GAPDH according to manufacturer′s instructions. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Microarray:

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions.

    In Silico:

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Four ~500 bp regions of the P . intermedia ATCC-25611 genome, densely populated with putative 5-methylcytosine recognition motifs identified by REBASE or from SMRT sequencing basemod analysis, were selected in-silico for primer design. .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Expressing:

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Quantitative rt-PCR —p38δ MAPK mRNA expression was quantitated using a Qiagen QuantiFast® MAPK13 Probe Assay with one-step rt-PCR and simultaneous detection of GAPDH according to manufacturer′s instructions. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Modification:

    Article Title: DNA Methylation of BDNF Gene in Schizophrenia
    Article Snippet: We treated 100 ng DNA samples with EpiMark Bisulfite Conversion Kit (Catalog No. E3318, New England Biolabs), in accordance with the manufacturer’s standard instructions. .. We treated 100 ng DNA samples with EpiMark Bisulfite Conversion Kit (Catalog No. E3318, New England Biolabs), in accordance with the manufacturer’s standard instructions.

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual. .. For PCR, sets of primer sequences described by Esteller et al. [ , ] were employed: for the unmethylated reaction; 5′-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3′ (U-93-F) and 5′-AACTCCACACTCTTCCAAAAACAAAACA-3′ (U-93-R) and for the methylated reaction; 5′-TTTCGACGTTCGTAGGTTTTCGC-3′ (M-81-F) and 5′-GCACTCTTCCGAAAACGAAACG-3′ (M-81-R).

    Derivative Assay:

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Genomic DNA was isolated from NSPs (derived from 2–3 week old C57BL/6 mice) using the all prep DNA/RNA mini kit (Qiagen). .. Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA).

    Hybridization:

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions.

    Biomarker Assay:

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: To assess the compatibility of DNA extracted using five prioritized protocols (AllPrep, QIAamp, RecAll, DNeasy, and GenJet) with enzyme-based downstream reactions, we performed methylation-specific PCR (MS-PCR), which is frequently employed in biomarker studies [ ]. .. As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA).

    Cell Culture:

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Yeast strain construction and cell culture were performed using standard methods. .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions.

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: Genomic DNA was extracted either from 5-15 mg of solid tissue or from 2 × 106 cultured cells using a Gentra Puregene DNA purification kit (QIAGEN, Germany) according to the manufacturer’s manual. .. DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual.

    Polymerase Chain Reaction:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. The CFTR promoter region was amplified using primers specific for the converted sequence (B1/B2, ).

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: Paragraph title: Bisulfite sequencing PCR (BSP) for validation of MSAP ... Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.).

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions. .. Detailed information regarding methodology and associated references are available in the .

    Article Title: Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector
    Article Snippet: 500 ng genomic DNA was treated with the Epitect Bisulfite Kit (Qiagen) or Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer’s instructions. .. A 206 bp region of the endogenous rat Oct4 promoter (−1495 to −1290) was amplified by PCR from bisulfite converted genomic DNA using primers BS-Oct4_F and BS-Oct4_R (see ).

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: Paragraph title: Methylation-specific PCR and bisulfite sequencing ... For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected.

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: PCR —1575bp and 1605bp fragments of p38δ MAPK mRNA, corresponding to positions 111–1686 and 82–1686 respectively of NCBI Reference Sequence NM_002754.4 were amplified from cellular cDNA using oligonucleotide primers P003F (5′-CGAGATCGGGTGCCCGGGAT-3′) or P002F (5′-CCGGAAAAAGGGCTTCTACAA-3′) and P001R (5′-CCGCCACAAGCTAAAAAGAG-3′). .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified. .. PCR-products were subjected to Sanger sequencing and methylation status of each CpG site was assessed semi-quantitatively from the sequence chromatograms .

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol. .. The Bcat1 promoter was amplified using the primers bcat1_for (5′- GGGTGTTAGGAATTAAATTTTTAATT-3′) and bcat1_rev (5′- CACAATAACTTTCTCTAAAACTCCC-3′).

    Article Title: DNA Methylation of BDNF Gene in Schizophrenia
    Article Snippet: Methylation-specific PCR was performed with primers specific for either methylated or unmethylated DNA. .. We treated 100 ng DNA samples with EpiMark Bisulfite Conversion Kit (Catalog No. E3318, New England Biolabs), in accordance with the manufacturer’s standard instructions.

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: Paragraph title: Methylation-specific PCR DNA assay ... As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA).

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual. .. For PCR, sets of primer sequences described by Esteller et al. [ , ] were employed: for the unmethylated reaction; 5′-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3′ (U-93-F) and 5′-AACTCCACACTCTTCCAAAAACAAAACA-3′ (U-93-R) and for the methylated reaction; 5′-TTTCGACGTTCGTAGGTTTTCGC-3′ (M-81-F) and 5′-GCACTCTTCCGAAAACGAAACG-3′ (M-81-R).

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified. .. PCR-products were subjected to Sanger sequencing and methylation status of each CpG site was assessed semi-quantitatively from the sequence chromatograms .

    DNA Sequencing:

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions. .. For bisulfite sequencing PCR (BSP) oligonucleotide primers 5′-TTGGGAGTTGGTTAGAAATGTA-3′ and 5′-AACAATACTTCCCAATTCCCT-3′ were designed to amplify a 270 bp fragment from bisulfite converted DNA.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Quantitative rt-PCR —p38δ MAPK mRNA expression was quantitated using a Qiagen QuantiFast® MAPK13 Probe Assay with one-step rt-PCR and simultaneous detection of GAPDH according to manufacturer′s instructions. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    MTT Assay:

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Migrated cells were treated with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (5 mg/mL) and absorbance read at 540 nm to calculate viable cell numbers as previously described [ ]. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Methylation:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Paragraph title: Methylation analysis via bisulfite sequencing ... Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions.

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: After bisulfite conversion, unmethylated cytosine was converted to uracil, while methylated cytosine or hydroxymethyl-cytosine was unchanged. .. Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.).

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: Paragraph title: Methylation-specific PCR and bisulfite sequencing ... For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected.

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Site-directed bisulfite sequencing was performed using primers designed with MethPrimer [ ], an open access design program for methylation mapping. .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: Quantitative rt-PCR —p38δ MAPK mRNA expression was quantitated using a Qiagen QuantiFast® MAPK13 Probe Assay with one-step rt-PCR and simultaneous detection of GAPDH according to manufacturer′s instructions. .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions. .. Methylation-specific PCR (MSP) is a method of assessing the methylation status of a group of CpG sites using individual pairs of primers specific for methylated (M) versus unmethylated (U) DNA [ ].

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: Paragraph title: Methylation analysis of the MYH7 -gene ... Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified.

    Article Title: Molecular effect of an OPTN common variant associated to Paget's disease of bone
    Article Snippet: Paragraph title: Methylation analysis by bisulfite conversion and sanger sequencing ... Sodium bisulfite treatment was performed on 1.5 μg of genomic DNA sample using the EpiMark® Bisulfite Conversion Kit [ ] (New England Biolabs Inc., Canada) following the manufacturers’ standard protocol.

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: The efficiency of enrichment of methylated DNA was compared to bisulfite sequencing experiments of promoter regions of a positive (branched chain-amino-acid aminotransferase cytosolic-like, Bcat1 , GeneID 100763658) and a negative (β-actin, Actb , GeneID 100689477) control. .. Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol.

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: Paragraph title: Methylation-specific PCR DNA assay ... As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA).

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA). .. Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA).

    Isolation:

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: Genomic DNA was isolated according to standard methods using a proteinase K digest. .. For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected.

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Genomic DNA was isolated from NSPs (derived from 2–3 week old C57BL/6 mice) using the all prep DNA/RNA mini kit (Qiagen). .. Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA).

    Size-exclusion Chromatography:

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB). .. Bisulfite PCR reactions were performed using ~100 ng of bisulfite-treated DNA, and TaKaRa EpiTaq HS (Clontech Laboratories, Inc., Mountain View, CA, USA).

    Labeling:

    Article Title: Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification
    Article Snippet: Genomic DNA was extracted from agarose plugs and labeled for microarray hybridization as previously described ( ). .. For bisulfite sequencing, genomic DNA was first treated with the EpiMark Bisulfite Conversion kit (New England Biolabs) and then PCR amplified using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs) according to the manufacturers instructions.

    Purification:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. For PCR amplification EpiMark Hot Start Taq DNA Polymerase was used (40 cycles, annealing temperature of 55°C).

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: For validation of the results of MSAP assays, the DALs of #23 and #52 (Supplemental Table ) was analyzed by BSP. .. Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.). .. The converted product was dissolved with 30 μl ddH2 O.

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: Genomic DNA was isolated according to standard methods using a proteinase K digest. .. For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. .. Methylation-specific PCR (MSP) and bisulfite sequencing were performed as previously described .

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB). .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol. .. The Bcat1 promoter was amplified using the primers bcat1_for (5′- GGGTGTTAGGAATTAAATTTTTAATT-3′) and bcat1_rev (5′- CACAATAACTTTCTCTAAAACTCCC-3′).

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
    Article Snippet: As preparation for MS-PCR, 100 ng of genomic DNA extracts was treated with sodium bisulfite, which converts unmethylated cytosine into uracil, and then column-purified according to the manufacturer’s protocol (EpiMark bisulfite conversion kit; NEB, USA). .. MS-PCR assays were carried out as published in our previous study [ ].

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: Purified DNA was quantified using a TBS-380 mini-fluorometer (Turner BioSystems, Sunnyvale, CA) using Hoechst 33258 dye and calf thymus DNA as a standard according to the manufacturer’s protocol. .. DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual.

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Genomic DNA was isolated from NSPs (derived from 2–3 week old C57BL/6 mice) using the all prep DNA/RNA mini kit (Qiagen). .. Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA). .. See Supplementary Information Materials and Methods.

    Sequencing:

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: The DNA methylation status of targeted cytosine could be determined by comparing the PCR product with the reference sequence. .. Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.).

    Article Title: Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector
    Article Snippet: 500 ng genomic DNA was treated with the Epitect Bisulfite Kit (Qiagen) or Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer’s instructions. .. PCR was performed with GoTaq DNA polymerase (Promega).

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Four ~500 bp regions of the P . intermedia ATCC-25611 genome, densely populated with putative 5-methylcytosine recognition motifs identified by REBASE or from SMRT sequencing basemod analysis, were selected in-silico for primer design. .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Hypermethylation of MAPK13 Promoter in Oesophageal Squamous Cell Carcinoma Is Associated with Loss of p38δ MAPK Expression
    Article Snippet: PCR —1575bp and 1605bp fragments of p38δ MAPK mRNA, corresponding to positions 111–1686 and 82–1686 respectively of NCBI Reference Sequence NM_002754.4 were amplified from cellular cDNA using oligonucleotide primers P003F (5′-CGAGATCGGGTGCCCGGGAT-3′) or P002F (5′-CCGGAAAAAGGGCTTCTACAA-3′) and P001R (5′-CCGCCACAAGCTAAAAAGAG-3′). .. Methylation analysis — The methylation status of DNA was determined using sodium bisulfite conversion with an EpiMark® Bisulfite Conversion Kit (New England BioLabs, Hertfordshire, UK) according to manufacturer′s instructions.

    Article Title: Molecular effect of an OPTN common variant associated to Paget's disease of bone
    Article Snippet: Paragraph title: Methylation analysis by bisulfite conversion and sanger sequencing ... Sodium bisulfite treatment was performed on 1.5 μg of genomic DNA sample using the EpiMark® Bisulfite Conversion Kit [ ] (New England Biolabs Inc., Canada) following the manufacturers’ standard protocol.

    Staining:

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. .. Published primers corresponding to CpG-rich regions of the 5' flanking region of human caspase-8 (nucleotides – 300 to – 697) , and TNSFR10A (DR4) genes (nucleotides – 27 to – 267) were used to amplify PCR products.

    Article Title: DNA Methylation of BDNF Gene in Schizophrenia
    Article Snippet: We treated 100 ng DNA samples with EpiMark Bisulfite Conversion Kit (Catalog No. E3318, New England Biolabs), in accordance with the manufacturer’s standard instructions. .. PCR mixture contained 10 ng of modified DNA, 2.0 mmol each of dATP, dGTP, dCTP, and dTTP, 1.0 pmol each of primer, 2.0 mmol MgCl2, 1× reaction buffer, and 1.0 U Taq polymerase.

    Mouse Assay:

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Genomic DNA was isolated from NSPs (derived from 2–3 week old C57BL/6 mice) using the all prep DNA/RNA mini kit (Qiagen). .. Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA).

    Plasmid Preparation:

    Article Title: Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector
    Article Snippet: 500 ng genomic DNA was treated with the Epitect Bisulfite Kit (Qiagen) or Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer’s instructions. .. PCR was performed with GoTaq DNA polymerase (Promega).

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. .. MSP amplicons were run on a 2% agarose gel, stained with ethidium bromide, and visualized by UV illumination.

    Software:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. PCR products were purified from agarose gel, subcloned using the CloneJET PCR cloning kit (Thermo Fisher Scientific), and sequenced using primer pJET1.2/FW (Thermo Fisher Scientific).

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Genomic sequence analysis was performed using the Seqbuilder and Seqman programs of the DNASTAR software package (DNASTAR, Madison, WI). .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified.

    Article Title: Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs
    Article Snippet: CpG-island detection in the MYH7 promotor and Bisulfite specific primer design was accomplished using the software Methyl Primer express. .. Genomic DNA was subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (NEB, USA) according to the suppliers’ instructions and subsequently the MYH7- promotor was PCR amplified.

    Agarose Gel Electrophoresis:

    Article Title: Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
    Article Snippet: Genomic DNA of CFBE41o- cells, 16HBE14o- cells and corrected cell clones was extracted with Qiagen Blood Mini kit, and 500 ng of genomic DNA was subjected to bisulfite treatment using EpiMark bisulfite conversion kit (New England Biolabs) according to the manufacturer’s instructions. .. For PCR amplification EpiMark Hot Start Taq DNA Polymerase was used (40 cycles, annealing temperature of 55°C).

    Article Title: Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells
    Article Snippet: For bisulfite sequencing analysis 1 μg of purified genomic DNA was treated with the EpiMark Bisulfite Conversion kit (NEB, Frankfurt, Germany) which, after bisulfite conversion and the subsequent desulphonation reaction, converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. .. Published primers corresponding to CpG-rich regions of the 5' flanking region of human caspase-8 (nucleotides – 300 to – 697) , and TNSFR10A (DR4) genes (nucleotides – 27 to – 267) were used to amplify PCR products.

    DNA Methylation Assay:

    Article Title: Overexpression of Human-Derived DNMT3A Induced Intergenerational Inheritance of Active DNA Methylation Changes in Rat Sperm
    Article Snippet: The DNA methylation status of targeted cytosine could be determined by comparing the PCR product with the reference sequence. .. Briefly, 2 μg sperm genomic DNA was converted and purified according to the instructions of the EpiMark bisulfite conversion kit (NEB, U.S.).

    Article Title: Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia
    Article Snippet: Site directed bisulfite sequencing was performed during identification of 5-methylcytosine DNA methylation. .. All bisulfite treatments of P . intermedia genomic DNA were performed using the EpiMark Bisulfite Conversion Kit (NEB).

    Article Title: The putative tumor suppressor gene EphA7 is a novel BMI-1 target
    Article Snippet: Paragraph title: DNA methylation analysis ... Bisulfite conversion and purification was done with the Epimark Bisulfite Conversion Kit following the instructions of the manufacturer (New England Biolabs, Ipswich, USA).

    DNA Purification:

    Article Title: Expression of O6-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Article Snippet: Genomic DNA was extracted either from 5-15 mg of solid tissue or from 2 × 106 cultured cells using a Gentra Puregene DNA purification kit (QIAGEN, Germany) according to the manufacturer’s manual. .. DNA (2.5 μg) was subjected to a bisulfite conversion reaction using an EpiMark bisulfite Conversion kit (New England Biolabs, Inc., Ipswich, MA) according to the manufacturer’s instruction manual.

    Gel Extraction:

    Article Title: Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells
    Article Snippet: Bisulfite conversion of 500 ng genomic DNA was carried out using the Epimark Bisulfite Conversion Kit (NEB) according to the manufacturer´s protocol. .. The Bcat1 promoter was amplified using the primers bcat1_for (5′- GGGTGTTAGGAATTAAATTTTTAATT-3′) and bcat1_rev (5′- CACAATAACTTTCTCTAAAACTCCC-3′).

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    New England Biolabs epimark bisulfite conversion kit
    Epimark Bisulfite Conversion Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 6 article reviews
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