e2612 protocol form  (New England Biolabs)


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    Structured Review

    New England Biolabs e2612 protocol form
    E2612 Protocol Form, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2612 protocol form/product/New England Biolabs
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e2612 protocol form - by Bioz Stars, 2019-08
    79/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Draft Genome Sequence of Chromatium okenii Isolated from the Stratified Alpine Lake Cadagno
    Article Snippet: Paragraph title: DNA Extraction, Sequencing and Genomic Analysis ... Genomic DNA (gDNA) was extracted with phenol/chloroform/isoamylalcohol solution (25:24:1, v /v ) adhering to the protocol provided by Pacific Biosciences in combination with phase lock gels for phase separation (VWR International, Radnor, USA). gDNA was concentrated and washed using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol from New England Biolabs .

    Functional Assay:

    Article Title: Draft Genome Sequence of Chromatium okenii Isolated from the Stratified Alpine Lake Cadagno
    Article Snippet: Genomic DNA (gDNA) was extracted with phenol/chloroform/isoamylalcohol solution (25:24:1, v /v ) adhering to the protocol provided by Pacific Biosciences in combination with phase lock gels for phase separation (VWR International, Radnor, USA). gDNA was concentrated and washed using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol from New England Biolabs . .. Concentration of the DNA was assessed using a Qubit UV/VIS absorption reader (Thermo Fisher Scientific, Rheinach, Switzerland).

    Multiplex Assay:

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs. .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs.

    Centrifugation:

    Article Title: Complete genome sequence of “Thiodictyon syntrophicum” sp. nov. strain Cad16T, a photolithoautotrophic purple sulfur bacterium isolated from the alpine meromictic Lake Cadagno
    Article Snippet: The key elements of the genome studied are listed in Table . .. Strain Cad16T was anaerobically grown in Pfennigs medium [ ] Cells were collected by centrifugation for 15 min at 10,600 g. DNA was extracted using phenol/chloroform/isoamylalcohol solution (25:24:1, v /v , Sigma, Buchs, Switzerland) following the protocol provided by Pacific Biosciences [ ] in combination with phase lock gels (VWR International). gDNA was purified using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol form New England Biolabs [ ]. .. Purity of the DNA was tested using the Qbit UV /VIS absorption reader (Thermo Fisher Scientific, Rheinach, Switzerland).

    Article Title: Complete genome sequence of “Thiodictyon syntrophicum” sp. nov. strain Cad16T, a photolithoautotrophic purple sulfur bacterium isolated from the alpine meromictic Lake Cadagno
    Article Snippet: The key elements of the genome studied are listed in Table . .. Strain Cad16T was anaerobically grown in Pfennigs medium [ ] Cells were collected by centrifugation for 15 min at 10,600 g. DNA was extracted using phenol/chloroform/isoamylalcohol solution (25:24:1, v / v , Sigma, Buchs, Switzerland) following the protocol provided by Pacific Biosciences [ ] in combination with phase lock gels (VWR International). gDNA was purified using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol form New England Biolabs [ ]. .. Purity of the DNA was tested using the Qbit U V /VIS absorption reader (Thermo Fisher Scientific, Rheinach, Switzerland).

    Construct:

    Article Title: Draft Genome Sequence of Chromatium okenii Isolated from the Stratified Alpine Lake Cadagno
    Article Snippet: Genomic DNA (gDNA) was extracted with phenol/chloroform/isoamylalcohol solution (25:24:1, v /v ) adhering to the protocol provided by Pacific Biosciences in combination with phase lock gels for phase separation (VWR International, Radnor, USA). gDNA was concentrated and washed using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol from New England Biolabs . .. The library construction and Single-Molecule Real-Time sequencing (SMRT) was done on the Pacific Biosciences RS II platform at the Functional Genomic Center Zurich, Zurich, Switzerland.

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs. .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs.

    Purification:

    Article Title: Complete genome sequence of “Thiodictyon syntrophicum” sp. nov. strain Cad16T, a photolithoautotrophic purple sulfur bacterium isolated from the alpine meromictic Lake Cadagno
    Article Snippet: The key elements of the genome studied are listed in Table . .. Strain Cad16T was anaerobically grown in Pfennigs medium [ ] Cells were collected by centrifugation for 15 min at 10,600 g. DNA was extracted using phenol/chloroform/isoamylalcohol solution (25:24:1, v /v , Sigma, Buchs, Switzerland) following the protocol provided by Pacific Biosciences [ ] in combination with phase lock gels (VWR International). gDNA was purified using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol form New England Biolabs [ ]. .. Purity of the DNA was tested using the Qbit UV /VIS absorption reader (Thermo Fisher Scientific, Rheinach, Switzerland).

    Article Title: Complete genome sequence of “Thiodictyon syntrophicum” sp. nov. strain Cad16T, a photolithoautotrophic purple sulfur bacterium isolated from the alpine meromictic Lake Cadagno
    Article Snippet: The key elements of the genome studied are listed in Table . .. Strain Cad16T was anaerobically grown in Pfennigs medium [ ] Cells were collected by centrifugation for 15 min at 10,600 g. DNA was extracted using phenol/chloroform/isoamylalcohol solution (25:24:1, v / v , Sigma, Buchs, Switzerland) following the protocol provided by Pacific Biosciences [ ] in combination with phase lock gels (VWR International). gDNA was purified using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol form New England Biolabs [ ]. .. Purity of the DNA was tested using the Qbit U V /VIS absorption reader (Thermo Fisher Scientific, Rheinach, Switzerland).

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: A glycerol stock was used to inoculate a tryptone-yeast extract (TY) agar plate ( ); the resulting single colony was incubated overnight in TY broth (28°C, 180 rpm). .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs. .. Total DNA was fragmented with medium-sized fragments of about 600 bp in a microTUBE Adaptive Focused Acoustics (AFA) fiber snap-cap tube using a Covaris S2 instrument.

    Incubation:

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: A glycerol stock was used to inoculate a tryptone-yeast extract (TY) agar plate ( ); the resulting single colony was incubated overnight in TY broth (28°C, 180 rpm). .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs.

    Sequencing:

    Article Title: Draft Genome Sequence of Chromatium okenii Isolated from the Stratified Alpine Lake Cadagno
    Article Snippet: Paragraph title: DNA Extraction, Sequencing and Genomic Analysis ... Genomic DNA (gDNA) was extracted with phenol/chloroform/isoamylalcohol solution (25:24:1, v /v ) adhering to the protocol provided by Pacific Biosciences in combination with phase lock gels for phase separation (VWR International, Radnor, USA). gDNA was concentrated and washed using AMPure beads (Agencourt, Beckman Coulter Life Sciences, Indianapolis, USA) following the E2612 protocol from New England Biolabs .

    Hybridization:

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: This region is a part of a modern center of alfalfa intrоgressive hybridization in northwest Kazakhstan, which has been suffering from manmade salinization since the 1960s ( , ). .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs.

    Software:

    Article Title: Draft Genome Sequence of Sinorhizobium meliloti Strain AK170
    Article Snippet: Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs. .. Genomic purification was done by using a Pacific Biosciences protocol with Phase Lock Gel (VWR International) and AMPure beads (Agencourt Bioscience, Beckman Coulter Life Sciences), following the E2612 protocol from New England BioLabs.

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    New England Biolabs e2612 protocol
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    New England Biolabs e2612 protocol form
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