Structured Review

New England Biolabs pminit
Pminit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pminit/product/New England Biolabs
Average 91 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pminit - by Bioz Stars, 2019-08
91/100 stars

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Related Articles

Clone Assay:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: DNA was amplified for 30 cycles in 25 ul reactions according to the manufacturer’s recommended cycling conditions using Q5 High Fidelity Polymerase (NEB catalog #M0494) to reduce stutter, generating blunt end products. .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Plasmids were transformed into 50ul of chemically competent E. coli (Lucigen E. cloni Chemically Competent Cells catalog #60108).

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: PAO1 genomic DNA was used as the template. .. PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB). .. The rsmA transcriptional fusions have been previously described ( ).

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Thermocycling was carried as above using Phusion polymerase with 5X GC Buffer without DMSO with same conditions as above for 35 cycles and run on a 2% agarose gel. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics). .. The sequences of these clones were submitted to Genbank (Accession numbers KX467569 and KX467570).

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: E. coli transformed clones were selected with agar plates containing ampicillin (100 μg/ml). .. Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor. .. Sixty-six single colonies of transformed E. coli clones were picked from selective plate containing ampicillin (100 μg/ml).

Amplification:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: DNA was amplified for 30 cycles in 25 ul reactions according to the manufacturer’s recommended cycling conditions using Q5 High Fidelity Polymerase (NEB catalog #M0494) to reduce stutter, generating blunt end products. .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202).

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Final amplified products were examined on 1.5% agarose (Agarose Unlimited) gels in 1X TAE for the above. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Agarose Gel Electrophoresis:

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Thermocycling was carried as above using Phusion polymerase with 5X GC Buffer without DMSO with same conditions as above for 35 cycles and run on a 2% agarose gel. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor. .. The presence of DNA inserts was determined by using the colony as DNA template for PCR with the primers provided with the kit.

Magnetic Beads:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: DNA was amplified for 30 cycles in 25 ul reactions according to the manufacturer’s recommended cycling conditions using Q5 High Fidelity Polymerase (NEB catalog #M0494) to reduce stutter, generating blunt end products. .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Plasmids were transformed into 50ul of chemically competent E. coli (Lucigen E. cloni Chemically Competent Cells catalog #60108).

Polymerase Chain Reaction:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: DNA was amplified for 30 cycles in 25 ul reactions according to the manufacturer’s recommended cycling conditions using Q5 High Fidelity Polymerase (NEB catalog #M0494) to reduce stutter, generating blunt end products. .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Plasmids were transformed into 50ul of chemically competent E. coli (Lucigen E. cloni Chemically Competent Cells catalog #60108).

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: PAO1 genomic DNA was used as the template. .. PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB). .. The rsmA transcriptional fusions have been previously described ( ).

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Thermocycling was carried as above using Phusion polymerase with 5X GC Buffer without DMSO with same conditions as above for 35 cycles and run on a 2% agarose gel. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics). .. The sequences of these clones were submitted to Genbank (Accession numbers KX467569 and KX467570).

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: E. coli transformed clones were selected with agar plates containing ampicillin (100 μg/ml). .. Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor. .. Sixty-six single colonies of transformed E. coli clones were picked from selective plate containing ampicillin (100 μg/ml).

Incubation:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Plasmids were transformed into 50ul of chemically competent E. coli (Lucigen E. cloni Chemically Competent Cells catalog #60108).

Cell Culture:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202).

Purification:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: DNA was amplified for 30 cycles in 25 ul reactions according to the manufacturer’s recommended cycling conditions using Q5 High Fidelity Polymerase (NEB catalog #M0494) to reduce stutter, generating blunt end products. .. Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Plasmids were transformed into 50ul of chemically competent E. coli (Lucigen E. cloni Chemically Competent Cells catalog #60108).

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB). .. To construct rsmY and rsmZ transcriptional fusions, the rsmYTFFEcoRI/rsmYTFR and rsmZTFF/rsmZTFR primer pairs, respectively, were used.

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Amplified products from the first PCR were cleaned using the MinElute PCR purification Kit (Qiagen) and used for the second round of PCR. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor. .. The presence of DNA inserts was determined by using the colony as DNA template for PCR with the primers provided with the kit.

Electrophoresis:

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor. .. PCR product (5 μl) were visualized by agarose gel electrophoresis and purified using AMPure beads.

Sequencing:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: Paragraph title: Sanger sequencing validation ... Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202).

Article Title: Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
Article Snippet: Paragraph title: Sanger sequencing of colony PCR products for KanR2 ... Post capture PCR products were cloned into pMiniT (NEB) by using NEB PCR cloning kit and used to transform chemically competent NEB 10-beta E. coli cells (NEB) as described by the vendor.

Generated:

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: Upstream DNA fragments containing promoter regions were generated by using primers listed in in conjunction with Q5 polymerase (New England BioLabs). .. PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB).

Gas Chromatography:

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Thermocycling was carried as above using Phusion polymerase with 5X GC Buffer without DMSO with same conditions as above for 35 cycles and run on a 2% agarose gel. .. The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Construct:

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB). .. PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB).

Expressing:

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Paragraph title: RT-PCR and qRT-PCR for DUX4 expression analysis ... The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Paragraph title: RT-PCR and qRT-PCR for DUX4 expression analysis ... The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Quantitative RT-PCR:

Article Title: Influence of Repressive Histone and DNA Methylation upon D4Z4 Transcription in Non-Myogenic Cells
Article Snippet: Paragraph title: RT-PCR and qRT-PCR for DUX4 expression analysis ... The PCR products were cloned into pMiniT (NEB) and inserts sequenced (Eurofins Genomics).

Plasmid Preparation:

Article Title: Genome-wide profiling of heritable and de novo STR variations
Article Snippet: Amplicons were purified on magnetic beads (Thermo Fisher Scientific ChargeSwitch PCR Clean-Up Kit, catalog #CS12000) and cloned into linearized pMiniT (NEB catalog #E1202). .. Individual colonies were selected and cultured overnight in 2mL LB + ampicillin (100ug/mL).

Article Title: The Pseudomonas aeruginosa Two-Component Regulator AlgR Directly Activates rsmA Expression in a Phosphorylation-Independent Manner
Article Snippet: PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB). .. PCR products were cloned into pMiniT (NEB) and then subcloned into miniCTX lacZ using the restriction enzymes HindIII and BamHI, HindIII and EcoRI, or KpnI and BamHI (NEB).

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    New England Biolabs pminit
    Pminit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pminit/product/New England Biolabs
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pminit - by Bioz Stars, 2019-08
    91/100 stars
      Buy from Supplier

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