e0540s  (New England Biolabs)


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    New England Biolabs e0540s
    E0540s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e0540s  (New England Biolabs)


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    New England Biolabs e0540s
    E0540s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e0540s a1 2 3 6 mannosidase new england biolabs  (New England Biolabs)


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    New England Biolabs e0540s a1 2 3 6 mannosidase new england biolabs
    E0540s A1 2 3 6 Mannosidase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    neuraminidase  (New England Biolabs)


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    New England Biolabs neuraminidase
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    neuraminidase bundle kit  (New England Biolabs)


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    New England Biolabs neuraminidase bundle kit
    Neuraminidase Bundle Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    o-glycosidase and neuraminidase bundle  (New England Biolabs)


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    New England Biolabs o-glycosidase and neuraminidase bundle
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    mui neuraminidase  (New England Biolabs)


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    New England Biolabs mui neuraminidase
    Effect of neuraminidase treatment on glycan detection <t>at</t> <t>EL4</t> cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 <t>mUI,</t> 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.
    Mui Neuraminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The T/Tn-Specific Helix pomatia Lectin Induces Cell Death in Lymphoma Cells Negative for T/Tn Antigens"

    Article Title: The T/Tn-Specific Helix pomatia Lectin Induces Cell Death in Lymphoma Cells Negative for T/Tn Antigens

    Journal: Cancers

    doi: 10.3390/cancers13174356

    Effect of neuraminidase treatment on glycan detection at EL4 cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 mUI, 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.
    Figure Legend Snippet: Effect of neuraminidase treatment on glycan detection at EL4 cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 mUI, 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.

    Techniques Used: Incubation, Labeling, Concentration Assay, Cell Culture

    neuraminidase  (New England Biolabs)


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    New England Biolabs neuraminidase
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    e0540s  (New England Biolabs)


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    New England Biolabs e0540s

    E0540s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Endoglycan plays a role in axon guidance by modulating cell adhesion"

    Article Title: Endoglycan plays a role in axon guidance by modulating cell adhesion

    Journal: eLife

    doi: 10.7554/eLife.64767


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Software

    o-glycosidase & neuraminidase bundle  (New England Biolabs)


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    New England Biolabs o-glycosidase & neuraminidase bundle
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    neuraminidase bundle  (New England Biolabs)


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    New England Biolabs neuraminidase bundle
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    New England Biolabs mui neuraminidase
    Effect of neuraminidase treatment on glycan detection <t>at</t> <t>EL4</t> cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 <t>mUI,</t> 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.
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    Effect of neuraminidase treatment on glycan detection <t>at</t> <t>EL4</t> cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 <t>mUI,</t> 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.
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    Image Search Results


    Effect of neuraminidase treatment on glycan detection at EL4 cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 mUI, 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.

    Journal: Cancers

    Article Title: The T/Tn-Specific Helix pomatia Lectin Induces Cell Death in Lymphoma Cells Negative for T/Tn Antigens

    doi: 10.3390/cancers13174356

    Figure Lengend Snippet: Effect of neuraminidase treatment on glycan detection at EL4 cell surface and lectin-induced cell death: ( A – C ) EL4 cells were incubated with (blue) or without (red) neuraminidase (20 mUI, 30 min, 37 °C) and labeled: ( A ) with anti-Tn (PE-αTn) or anti-T (PE-αT) mouse monoclonal antibodies or ( B , C ) with FITC-lectins: HPA (FITC-HPA), MorG (FITC-MorG), or ConA (FITC-ConA). ( A ) Red arrow indicates autofluorescence pic position (grey curve); ( B ) representative results of two independent experiments with 5 μg/mL of labeled lectin, with blue curve corresponding to neuraminidase-treated cells; and ( C ) lectin concentration effect on labelling. ( D ) EL4 cells were incubated with (+ Nase) or without (− Nase) neuraminidase, washed, and cultured for 48 h with 2.5 μg/mL of HPA, MorG, or ConA, then cell death was analyzed using cytofluorimetry. Results are means ± SD of three independent experiments.

    Article Snippet: EL4 cells were treated with 20 mUI Neuraminidase (#E0540S New England Biolabs, Ipswich, MA, USA) for 30 min at 37 °C in PBS, then washed before using.

    Techniques: Incubation, Labeling, Concentration Assay, Cell Culture