dna quantification  (Worthington Biochemical)


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    Name:
    Deoxyribonucleic Acid E coli
    Description:
    Supplied as a dried powder purified from E coli Type B cells ATCC 11303 as described by Marmur J Mol Biol 3 208 1961
    Catalog Number:
    LS004449
    Price:
    104
    Source:
    E. coli
    Size:
    10 mg
    Cas Number:
    9007.49.2
    Buy from Supplier


    Structured Review

    Worthington Biochemical dna quantification
    Viability of <t>hMSCs</t> encapsulated in tethered TGF β hydrogels. <t>DNA</t> content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Supplied as a dried powder purified from E coli Type B cells ATCC 11303 as described by Marmur J Mol Biol 3 208 1961
    https://www.bioz.com/result/dna quantification/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna quantification - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells"

    Article Title: Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells

    Journal: Drug Delivery and Translational Research

    doi: 10.1007/s13346-012-0090-2

    Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Figure Legend Snippet: Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

    Techniques Used: Cell Culture, Negative Control, Positive Control

    2) Product Images from "3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine"

    Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine

    Journal: Nucleic Acids Research

    doi:

    MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.
    Figure Legend Snippet: MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Techniques Used: Modification, Sequencing

    Related Articles

    other:

    Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine
    Article Snippet: SVPDE and E.coli DNA polymerase I were purchased from Worthington Biochemicals (Lakewood, NJ).

    Mouse Assay:

    Article Title: Human Immunodeficiency Virus-Like Particles Activate Multiple Types of Immune Cells
    Article Snippet: In vivo expansion of DCs in BALB/c mice was carried out by injecting plasmid DNA that expresses the human FL extracellular domain as described by . .. After 9 days, spleens were collected from mice that received FL DNA and single cell suspensions were prepared after treatments with type IV collagenase (Worthington) and lysis of red blood cells. .. Single cell suspensions were incubated with CD11c (N418) microbeads and CD11c+ DC cells were obtained by passing through magnetic columns according to the manufacturer’s instructions (Miltenyi Biotec Inc. Auburn, CA).

    Lysis:

    Article Title: Human Immunodeficiency Virus-Like Particles Activate Multiple Types of Immune Cells
    Article Snippet: In vivo expansion of DCs in BALB/c mice was carried out by injecting plasmid DNA that expresses the human FL extracellular domain as described by . .. After 9 days, spleens were collected from mice that received FL DNA and single cell suspensions were prepared after treatments with type IV collagenase (Worthington) and lysis of red blood cells. .. Single cell suspensions were incubated with CD11c (N418) microbeads and CD11c+ DC cells were obtained by passing through magnetic columns according to the manufacturer’s instructions (Miltenyi Biotec Inc. Auburn, CA).

    Polymerase Chain Reaction:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

    Clone Assay:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

    Plasmid Preparation:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

    Construct:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

    Transfection:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

    Sequencing:

    Article Title: Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium
    Article Snippet: All MT1 and MT2 RT-PCR products were normalized to the corresponding β-actin RT-PCR results that served as an internal control to ensure an approximate ratio of MTs mRNA. .. PCR products were cloned using pUCm-T vector, The constructed plasmids were transfected into JM109, positive colonies were selected and the DNA sequence was analysed using a DNA sequencer (Worthington Biochemical Co.). .. Testicular interstitial cells were suspended in 500 μL of 10 mM Tris-HCL, pH7.4, and lysed by sonication (3 × 10 s) on ice.

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  • 94
    Worthington Biochemical dna quantification
    Viability of <t>hMSCs</t> encapsulated in tethered TGF β hydrogels. <t>DNA</t> content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Dna Quantification, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna quantification - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    86
    Worthington Biochemical vasquez e
    Viability of <t>hMSCs</t> encapsulated in tethered TGF β hydrogels. <t>DNA</t> content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Vasquez E, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vasquez e/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vasquez e - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Worthington Biochemical worthington e l
    Viability of <t>hMSCs</t> encapsulated in tethered TGF β hydrogels. <t>DNA</t> content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Worthington E L, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/worthington e l/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    worthington e l - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

    Journal: Drug Delivery and Translational Research

    Article Title: Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells

    doi: 10.1007/s13346-012-0090-2

    Figure Lengend Snippet: Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

    Article Snippet: DNA quantification of encapsulated hMSCs Immediately following photoencapsulation, cell-laden hydrogels (n = 3) were placed into an enzymatic digest buffer (125 μg/mL papain (Worthington Biochemical), 10 mM cysteine) overnight at 60 °C, then frozen prior to analysis.

    Techniques: Cell Culture, Negative Control, Positive Control

    MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Journal: Nucleic Acids Research

    Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine

    doi:

    Figure Lengend Snippet: MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Article Snippet: SVPDE and E.coli DNA polymerase I were purchased from Worthington Biochemicals (Lakewood, NJ).

    Techniques: Modification, Sequencing