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erythromycin  (Gold Biotechnology Inc)


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    Structured Review

    Gold Biotechnology Inc erythromycin
    (A) Design of tlpD insert cassette for transformation into H. pylori . This cassette contains the tlpD sequence, a 3xflag tag and <t>erythromycin</t> ( ery ) resistance sequences that are flanked by rdxA homologous regions for integration into the H. pylori genome at the rdxA locus. (B) PCR of erythromycin resistant H. pylori isolates using primers that flank the rdxA locus. The nomenclature above each band indicates the amplified product. (-); PCR no template control. kan-sacB ; kanamycin resistance and sucrose sensitivity selection marker present in the H. pylori transformation background and serves as a negative control. tlpD , ΔN4, ΔN5, ΔC3, ΔC4 are the versions of tlpD on the cassette that were PCR amplified.
    Erythromycin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erythromycin/product/Gold Biotechnology Inc
    Average 93 stars, based on 1 article reviews
    erythromycin - by Bioz Stars, 2025-05
    93/100 stars

    Images

    1) Product Images from "The C-terminus of the TlpD cytoplasmic chemoreceptor promotes polar localization and function"

    Article Title: The C-terminus of the TlpD cytoplasmic chemoreceptor promotes polar localization and function

    Journal: bioRxiv

    doi: 10.1101/2024.11.08.622596

    (A) Design of tlpD insert cassette for transformation into H. pylori . This cassette contains the tlpD sequence, a 3xflag tag and erythromycin ( ery ) resistance sequences that are flanked by rdxA homologous regions for integration into the H. pylori genome at the rdxA locus. (B) PCR of erythromycin resistant H. pylori isolates using primers that flank the rdxA locus. The nomenclature above each band indicates the amplified product. (-); PCR no template control. kan-sacB ; kanamycin resistance and sucrose sensitivity selection marker present in the H. pylori transformation background and serves as a negative control. tlpD , ΔN4, ΔN5, ΔC3, ΔC4 are the versions of tlpD on the cassette that were PCR amplified.
    Figure Legend Snippet: (A) Design of tlpD insert cassette for transformation into H. pylori . This cassette contains the tlpD sequence, a 3xflag tag and erythromycin ( ery ) resistance sequences that are flanked by rdxA homologous regions for integration into the H. pylori genome at the rdxA locus. (B) PCR of erythromycin resistant H. pylori isolates using primers that flank the rdxA locus. The nomenclature above each band indicates the amplified product. (-); PCR no template control. kan-sacB ; kanamycin resistance and sucrose sensitivity selection marker present in the H. pylori transformation background and serves as a negative control. tlpD , ΔN4, ΔN5, ΔC3, ΔC4 are the versions of tlpD on the cassette that were PCR amplified.

    Techniques Used: Transformation Assay, Sequencing, Amplification, Control, Selection, Marker, Negative Control



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    Image Search Results


    (A) Design of tlpD insert cassette for transformation into H. pylori . This cassette contains the tlpD sequence, a 3xflag tag and erythromycin ( ery ) resistance sequences that are flanked by rdxA homologous regions for integration into the H. pylori genome at the rdxA locus. (B) PCR of erythromycin resistant H. pylori isolates using primers that flank the rdxA locus. The nomenclature above each band indicates the amplified product. (-); PCR no template control. kan-sacB ; kanamycin resistance and sucrose sensitivity selection marker present in the H. pylori transformation background and serves as a negative control. tlpD , ΔN4, ΔN5, ΔC3, ΔC4 are the versions of tlpD on the cassette that were PCR amplified.

    Journal: bioRxiv

    Article Title: The C-terminus of the TlpD cytoplasmic chemoreceptor promotes polar localization and function

    doi: 10.1101/2024.11.08.622596

    Figure Lengend Snippet: (A) Design of tlpD insert cassette for transformation into H. pylori . This cassette contains the tlpD sequence, a 3xflag tag and erythromycin ( ery ) resistance sequences that are flanked by rdxA homologous regions for integration into the H. pylori genome at the rdxA locus. (B) PCR of erythromycin resistant H. pylori isolates using primers that flank the rdxA locus. The nomenclature above each band indicates the amplified product. (-); PCR no template control. kan-sacB ; kanamycin resistance and sucrose sensitivity selection marker present in the H. pylori transformation background and serves as a negative control. tlpD , ΔN4, ΔN5, ΔC3, ΔC4 are the versions of tlpD on the cassette that were PCR amplified.

    Article Snippet: For the selection of H. pylori mutants, 13 μg/mL chloramphenicol, 15 μg/mL kanamycin (Fisher BioReagents), or 25 μg/mL erythromycin (GoldBio) was used.

    Techniques: Transformation Assay, Sequencing, Amplification, Control, Selection, Marker, Negative Control