Journal: Biophysical reports
Article Title: Comparative Ca2+ channel contributions to intracellular Ca2+ levels in the circadian clock
Figure Lengend Snippet: Effects of Ca 2+ channel inhibitors on peak and trough Ca 2+ concentration. ( A and B ) Time course of the change in Ca 2+ concentration (ΔCa 2+ = Ca 2+ – average Ca 2+ from 2-min baseline) before and after the application of vehicle control (Veh) or Ca 2+ channel inhibitors at the peak ( A ) and trough ( B ). Data are mean ± SEM. ( C and D ) Plots of median, 25th and 75th percentile ( boxes ), and minimal and maximal ( whiskers ) changes in Ca 2+ concentration for individual slices quantified from 9 to 10 min after drugs were applied at the peak ( C ) and trough ( D ). Inhibition of L-type Ca 2+ channels with nimodipine (Nim, 10 μ M) or inhibition of N/P/Q/R/T-type Ca 2+ channels with VGC (a mixture of 3 μ M ConoGVIA, 200 nM AgaIVA, 30 μ M nickel, and 1 μ M TTA-P2) did not significantly affect peak or trough Ca 2+ levels compared to Veh. Inhibition of ryanodine receptors with dantrolene (Dan, 10 μ M), inhibition of SERCA-ATPase with cyclopiazonic acid (CPA, 10 μ M) and combined inhibition of voltage-gated Ca 2+ channels and ryanodine receptors with a cocktail containing Dan, Nim, and VGC (cocktail X) significantly decreased Ca 2+ at peak and trough. * p
Article Snippet: Pharmacological reagents were used at final concentrations of 10 μ M nimodipine (Nim; N150; Alomone Labs, Jerusalem, Israel), 10 μ M dantrolene (Dan; D9175; Sigma-Aldrich), 30 μ M CPA (C-750; Alomone Labs), 3 μ M ω -conotoxin GVIA (ConoGVIA; C-300; Alomone Labs), 200 nM ω -agatoxin IVA (AgaIVA; STA-500; Alomone Labs), 30 μ M NiCl2 (Ni2+ ; 22387; Sigma-Aldrich), 10 μ M ionomycin (407951; Sigma-Aldrich), 10 μ M rotenone (R8875; Sigma-Aldrich), and 50 mM KCl (P9333; Sigma-Aldrich).
Techniques: Concentration Assay, Inhibition