deoxyribonuclease (Worthington Biochemical)

Worthington Biochemical is a verified supplier

Worthington Biochemical manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

ls002004

Price:

Size:

5 mg

Source:

Bovine Pancreas

Cas Number:

9003.98.9

Buy from Supplier

Structured Review

Worthington Biochemical deoxyribonuclease
Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/deoxyribonuclease/product/Worthington Biochemical
Average 99 stars, based on 160 article reviews
Price from $9.99 to $1999.99

deoxyribonuclease - by Bioz Stars, 2020-05

99/100 stars

Related Products / Commonly Used Together

rna
pbs
total rna
cdna
dispase
mgcl2
edta
collagenase d
trizol reagent
rneasy mini kit
trizol
hbss
dmem
cdna synthesis
rpmi

Images

In Vitro:

Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

Positive Control:

Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

Labeling:

Article Title: Further Unraveling the Regulatory Twist by Elucidating Metabolic Coinducer-Mediated CbbR-cbbI Promoter Interactions in Rhodopseudomonas palustris CGA010
Article Snippet: .. The fluorescently labeled FP probe (459 bp), consisting of the entire intergenic region between cbbSR and cbbL and extending 216 bp into cbbSR and 44 bp into cbbL on either side of the intergenic region, was incubated with 100 nM His-tagged CbbR and then digested with DNase I. .. The digested DNA fragments were analyzed by capillary electrophoresis using an automated sequencer.

Concentration Assay:

Incubation:

Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

other:

Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).

Blocking Assay:

Expressing:

Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
Article Snippet: .. Analysis of nuclease sensitivity ( ) of the genomic histone HIST2H4 locus to DNase I reveals changes in chromatin structure that accompany increased histone gene expression in hES cells. ..