dnase (Worthington Biochemical)

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Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

LS002004

Price:

Source:

Bovine Pancreas

Size:

5 mg

Cas Number:

9003.98.9

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Structured Review

Worthington Biochemical dnase
Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/dnase/product/Worthington Biochemical
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

dnase - by Bioz Stars, 2021-07

99/100 stars

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Concentration Assay:

Article Title: NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium
Article Snippet: .. DNase I (Worthington Biochemical) was added to a final concentration of 130 μg/ml. .. To aid the removal of cut DNA, (NH4 )2 SO4 was added to a final concentration of 0.25 M and the samples were incubated for 5 min at room temperature.

Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
Article Snippet: Briefly, nuclei were isolated and treated with increasing concentrations of DNase I (Worthington Biochemical Co, Lakewood, NJ). .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

other:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

End Labeling:

Article Title: Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter
Article Snippet: The positions of the MNase cleavages within chromatin of the HPRT promoter region of permeabilized cells relative to a downstream Bcl I site in the first intron of the HPRT gene were mapped using a 400-bp hybridization probe located just upstream of the Bcl I site (Fig. ). .. To determine the positions of DNase I-hypersensitive sites relative to MNase cleavage sites, NP-40-permeabilized cells containing the active HPRT allele were treated with increasing concentrations of DNase I and the DNase I-hypersensitive sites in chromatin of the HPRT promoter relative to the same Bcl I site were also mapped by indirect end labeling using the same hybridization probe. .. Figure shows the Southern blot analysis of the DNase I and MNase cleavage patterns on the inactive and active HPRT promoters in permeabilized 8121 and 4.12 cells, respectively.

Hybridization:

Expressing:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: In summary, the present study provided novel insight into the central role of DNase I in high glucose-induced pancreatic β-cell apoptosis. .. It was demonstrated that high glucose was able to increase the expression of DNase I, and that this may aggravate β-cell apoptosis. ..

Cell Culture:

Article Title: Evaluation of the Functional Involvement of Human Immunodeficiency Virus Type 1 Integrase in Nuclear Import of Viral cDNA during Acute Infection
Article Snippet: Pseudotype viruses were prepared by cotransfection of 293T cells with the pNL43lucΔenv vector together with the MuLV envelope expression vector (pJD-1). .. After treatment with DNase I (40 μg/ml; Worthington), each virus (70 ng of p24) was inoculated into HeLa cells and cultured at 37°C for 6 h. The cells were washed with PBS and resuspended in fresh medium (DMEM plus 10% FBS). ..

Positive Control:

Staining:

Article Title: NuMA Influences Higher Order Chromatin Organization in Human Mammary Epithelium
Article Snippet: To verify that the DNase I treatment mentioned in the previous paragraph indeed affected chromatin components, we analyzed the staining pattern of chromatin markers acetyl-H4 and H4K20m ( ; ) after DNase I treatment. .. Staining for these proteins was almost totally eliminated in cells treated with DNase I, whereas the staining pattern for SC35, indicative of nonchromatin splicing speckles, seemed unaltered ( , G–J). .. The quantitative analysis of the distribution of NuMA in differentiated S1 cells that have not undergone DNase I treatment reveals that NuMA staining foci are abundant in the midnuclear region ( D; ).