1 calf thymus dna (Worthington Biochemical)


Structured Review
1 Calf Thymus Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 calf thymus dna/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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ambient dna (Worthington Biochemical)


Structured Review
Ambient Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ambient dna/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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sds dna page (Worthington Biochemical)


Structured Review

Sds Dna Page, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sds dna page/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic Bacterium Holospora obtusa Secreted to the Outside of the Bacterium during the Early Infection Process Binds Weakly to the Macronuclear DNA of the Host Paramecium caudatum"
Article Title: A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic Bacterium Holospora obtusa Secreted to the Outside of the Bacterium during the Early Infection Process Binds Weakly to the Macronuclear DNA of the Host Paramecium caudatum
Journal: Microorganisms
doi: 10.3390/microorganisms11010155

Figure Legend Snippet: SDS-DNA PAGE of IF cells of H. obtusa. Calf thymus DNA was used. ( a ) CBB stained gel; ( b ) immunoblot with mAb3D1B9C4 specific for PRP1; ( c ) EB stained gel. Arrows, PRP1. Arrowheads, other EB-staining negative bands showing positions of DNA-protein complexes (see ).
Techniques Used: IF-cells, Staining, Western Blot

Figure Legend Snippet: SDS-PAGE and immunoblots of fractions of DNA affinity column chromatography. Calf thymus DNA was used. ( a ) Fractions 1–10 eluted with Na, K-PB; ( b ) fractions 1′–10′ eluted with Na, K-PB containing 0.2 M NaCl. Upper, silver-stained gel. Lower, immunoblot with mAb3D1B9C4. ( c ) High magnification of a fraction number 3′ using silver staining. Not only PRP1 but also several silver staining bands were also confirmed. ( d ) CBB stained gel of IF cells of H. obtusa . Arrow, PRP1. Arrowheads, other bands eluted. Bands in ( c ) match the bands in ( d ).
Techniques Used: SDS Page, Western Blot, Affinity Column, Chromatography, Staining, Silver Staining, IF-cells

Figure Legend Snippet: SDS-DNA PAGE of IF cells of H. obtusa . P. caudatum DNA was used. ( a ) CBB stained gel. ( b ) Immunoblot with mAb3D1B9C4 specific for PRP1. ( c ) EB stained gel. Arrows, 63-kDa PRP1. It should be noted that not only PRP1 but also many EB-negative bands that show the positions of DNA-protein complexes appeared (see ).
Techniques Used: IF-cells, Staining, Western Blot

Figure Legend Snippet: DNA affinity chromatography of H. obtusa IFs. P. caudatum DNA was used. ( a ) Silver stained SDS-PAGE gel; ( b ) immunoblotting with the monoclonal antibody mAb3D1B9C4 specific for the 63-kDa periplasmic protein PRP1 of H. obtusa . Lane 1, supernatant of sonicated H. obtusa before applying to DNA affinity chromatography column; lane 2, first elute of supernatant of sonicated H. obtusa after applying to the column; lane 3, first elute by elution buffer containing 0.02 M NaCl; lane 4, first eluate by 0.1 M NaCl elution buffer; lane 5, first eluate by 0.2 M NaCl elution buffer; lane 6, first eluate by 0.3 M NaCl elution buffer; lane 7, first eluate by 2 M NaCl elution buffer; arrows, PRP1. Eluates from each column were collected in every 200 μL, and the first eluate from each column was used for SDS-PAGE and immunoblot with mAb. Note that not only PRP1 but also several silver stained bands were confirmed in lane 4 of ( a ).
Techniques Used: Affinity Chromatography, Staining, SDS Page, Western Blot, Sonication, Affinity Column
p caudatum whole genome dna (Worthington Biochemical)


Structured Review

P Caudatum Whole Genome Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p caudatum whole genome dna/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic Bacterium Holospora obtusa Secreted to the Outside of the Bacterium during the Early Infection Process Binds Weakly to the Macronuclear DNA of the Host Paramecium caudatum"
Article Title: A 63-kDa Periplasmic Protein of the Endonuclear Symbiotic Bacterium Holospora obtusa Secreted to the Outside of the Bacterium during the Early Infection Process Binds Weakly to the Macronuclear DNA of the Host Paramecium caudatum
Journal: Microorganisms
doi: 10.3390/microorganisms11010155

Figure Legend Snippet: SDS-DNA PAGE of IF cells of H. obtusa . P. caudatum DNA was used. ( a ) CBB stained gel. ( b ) Immunoblot with mAb3D1B9C4 specific for PRP1. ( c ) EB stained gel. Arrows, 63-kDa PRP1. It should be noted that not only PRP1 but also many EB-negative bands that show the positions of DNA-protein complexes appeared (see ).
Techniques Used: IF-cells, Staining, Western Blot

Figure Legend Snippet: DNA affinity chromatography of H. obtusa IFs. P. caudatum DNA was used. ( a ) Silver stained SDS-PAGE gel; ( b ) immunoblotting with the monoclonal antibody mAb3D1B9C4 specific for the 63-kDa periplasmic protein PRP1 of H. obtusa . Lane 1, supernatant of sonicated H. obtusa before applying to DNA affinity chromatography column; lane 2, first elute of supernatant of sonicated H. obtusa after applying to the column; lane 3, first elute by elution buffer containing 0.02 M NaCl; lane 4, first eluate by 0.1 M NaCl elution buffer; lane 5, first eluate by 0.2 M NaCl elution buffer; lane 6, first eluate by 0.3 M NaCl elution buffer; lane 7, first eluate by 2 M NaCl elution buffer; arrows, PRP1. Eluates from each column were collected in every 200 μL, and the first eluate from each column was used for SDS-PAGE and immunoblot with mAb. Note that not only PRP1 but also several silver stained bands were confirmed in lane 4 of ( a ).
Techniques Used: Affinity Chromatography, Staining, SDS Page, Western Blot, Sonication, Affinity Column
1 calf thymus dna (Worthington Biochemical)


Structured Review
1 Calf Thymus Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 calf thymus dna/product/Worthington Biochemical
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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phage η genomic dna (Worthington Biochemical)


Structured Review
Phage η Genomic Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phage η genomic dna/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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residual dna contamination (Worthington Biochemical)


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Residual Dna Contamination, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/residual dna contamination/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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dna cellulose (Worthington Biochemical)


Structured Review
Dna Cellulose, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna cellulose/product/Worthington Biochemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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genomic dna contamination (Worthington Biochemical)


Structured Review
Genomic Dna Contamination, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna contamination/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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t4 dna polymerase (Worthington Biochemical)


Structured Review
T4 Dna Polymerase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 dna polymerase/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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native double stranded calf thymus ct dna (Worthington Biochemical)


Structured Review

Native Double Stranded Calf Thymus Ct Dna, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/native double stranded calf thymus ct dna/product/Worthington Biochemical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers"
Article Title: The Inhibition of Anti-DNA Binding to DNA by Nucleic Acid Binding Polymers
Journal: PLoS ONE
doi: 10.1371/journal.pone.0040862

Figure Legend Snippet: The ability of polymers PAMAM, HDMBr, and CDP to inhibit binding of a murine monoclonal anti-DNA antibody QB1 to DNA was tested by ELISA. Binding of QB1 at 35 ng/ml final concentration was assayed by ELISA in the presence of PAMAM, HDMBr, or CDP at final concentrations from 10 ng/ml to 1,250 ng/ml. Control wells had buffer alone. Antibody was measured as described in Experimental Procedures. The OD 450 values of 3 wells of each polymer concentration or of 6 wells without polymer were averaged. Each point shown is 100% x average OD 450 with polymer/average OD 450 without polymer. Circles show data for PAMAM; squares show data for HDMBr; triangles show data for CDP.
Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

Figure Legend Snippet: The ability of polymers PAMAM, HDMBr, and CDP to inhibit DNA binding by anti-DNA antibodies in three SLE patient plasmas to DNA was tested by ELISA. Binding by antibodies in Plasma 1 (final dilution 1/1,000), Plasma 2 (final dilution 1/2,800), and Plasma 3 (final dilution 1/3,000) was assayed in the presence of PAMAM, HDMBr, or CDP at final concentrations ranging from 300 ng/ml to 10,000 ng/ml or with dilution buffer alone. Antibody levels were determined by ELISA as described in . The OD 450 values of 2 wells for each condition were averaged. Each point shown is 100% x average OD 450 with polymer/average OD 450 without polymer. Circles show data for Plasma 1; squares show data for Plasma 2; triangles show data for Plasma 3.
Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: Native CT DNA was biotinylated and then captured in ELISA plate wells coated with streptavidin. The binding of Plasma 1 (final dilution 1/3,500), Plasma 2 (final dilution 1/8,000), and Plasma 3 (final dilution 1/4,500) was measured by ELISA in the presence of PAMAM, HDMBr, or CDP at final concentrations from 80 ng/ml to 10,000 ng/ml or with dilution buffer alone. The OD 450 values of 2 wells for each condition were averaged. Each point shown is 100% x average OD 450 with polymer/average OD 450 without polymer. Circles show data for Plasma 1; squares show data for Plasma 2; triangles show data for Plasma 3.
Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay

Figure Legend Snippet: In this assay, 50 µl/well of Plasma 1 diluted 1/1,700, Plasma 2 diluted 1/3,950, and Plasma 3 diluted 1/2,250 were incubated in wells of microtiter plates with biotinylated DNA bound to streptavidin. After 1 hour to allow the formation of immune complexes, 50 µl of dilutions of PAMAM, HDMBr, or CDP or 50 µl of dilution buffer alone were then added to produce concentrations of the polymers of 10,000 ng/ml, 2,500 ng/ml, 620 ng/ml, 160 ng/ml or 0 ng/ml. Antibody binding was then determined by ELISA. The OD 450 values of 2 wells for each condition were averaged. Each point shown is 100% x average OD 450 with polymer/average OD 450 without polymer. Circles show data for Plasma 1; squares show data for Plasma 2; triangles show data for Plasma 3.
Techniques Used: Incubation, Binding Assay, Enzyme-linked Immunosorbent Assay