pcr dna marker  (worthington biochemical)

Journal: bioRxiv

Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi

doi: 10.1101/2021.07.02.450927

Figure Lengend Snippet: Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. PCR DNA marker (NEB) was loaded in lane 10.

Article Snippet: 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.).

Techniques: Titration, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

Journal: bioRxiv

Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi

doi: 10.1101/2021.07.02.450927

Figure Lengend Snippet: Mononucleosome sample and total number of paired-reads. (A) Chromatin digestions from the CL Brener replicate 1 experiment analyzed in a 2% agarose gel. The sample loaded in lane 4 was used for the experiment. PCR DNA (NEB) marker was loaded in lane 1. (B) Total number of paired-reads obtained in each replicate experiment.

Article Snippet: 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.).

Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

Journal: Drug Delivery and Translational Research

Article Title: Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells

doi: 10.1007/s13346-012-0090-2

Figure Lengend Snippet: Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

Article Snippet: DNA quantification of encapsulated hMSCs Immediately following photoencapsulation, cell-laden hydrogels (n = 3) were placed into an enzymatic digest buffer (125 μg/mL papain (Worthington Biochemical), 10 mM cysteine) overnight at 60 °C, then frozen prior to analysis.

Techniques: Cell Culture, Negative Control, Positive Control

Journal: Nucleic Acids Research

Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine

doi:

Figure Lengend Snippet: MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

Article Snippet: SVPDE and E.coli DNA polymerase I were purchased from Worthington Biochemicals (Lakewood, NJ).

Techniques: Modification, Sequencing

Journal: Clinical and Experimental Immunology

Article Title: RORγt expression in Tregs promotes systemic lupus erythematosus via IL‐17 secretion, alteration of Treg phenotype and suppression of Th2 responses

doi: 10.1111/cei.12905

Figure Lengend Snippet: (a) Quantification of immunoglobulin (Ig)G anti‐ds‐DNA antibodies, as well as the indicated IgG subclasses from serum of forkhead box protein 3 (FoxP3) Cre wild‐type and FoxP3 Cre × retinoic acid receptor‐related orphan nuclear receptor (ROR)C fl/fl mice in serial dilutions by enzyme‐linked immunosorbent assay (ELISA). (b) Quantification of serum anti‐U1‐ribonucleoprotein (RNP) autoantibodies of the indicated IgG subclasses in serial dilutions by ELISA. (c) Immunohistochemical staining and quantification of glomerular complement C3 deposition (original magnification ×400). (d) Immunohistochemical staining and quantification of glomerular mouse IgG (mIgG) deposition (original magnification ×400). Squares represent individual animals, horizontal lines indicate means.

Article Snippet: Circulating anti‐ds‐DNA and anti‐U1‐ribonucleoprotein (RNP) antibodies from serum were analysed by ELISA at the indicated dilutions after coating microtitre plates with either poly‐L‐lysine (Sigma‐Aldrich) and calf thymus DNA (Worthington) or U1‐RNP (Arotec).

Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining