pcr dna marker  (worthington biochemical)


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    worthington biochemical pcr dna marker
    Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. <t>PCR</t> <t>DNA</t> marker (NEB) was loaded in lane 10.
    Pcr Dna Marker, supplied by worthington biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr dna marker/product/worthington biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr dna marker - by Bioz Stars, 2021-07
    86/100 stars

    Images

    1) Product Images from "Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi"

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi

    Journal: bioRxiv

    doi: 10.1101/2021.07.02.450927

    Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. PCR DNA marker (NEB) was loaded in lane 10.
    Figure Legend Snippet: Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. PCR DNA marker (NEB) was loaded in lane 10.

    Techniques Used: Titration, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

    Mononucleosome sample and total number of paired-reads. (A) Chromatin digestions from the CL Brener replicate 1 experiment analyzed in a 2% agarose gel. The sample loaded in lane 4 was used for the experiment. PCR DNA (NEB) marker was loaded in lane 1. (B) Total number of paired-reads obtained in each replicate experiment.
    Figure Legend Snippet: Mononucleosome sample and total number of paired-reads. (A) Chromatin digestions from the CL Brener replicate 1 experiment analyzed in a 2% agarose gel. The sample loaded in lane 4 was used for the experiment. PCR DNA (NEB) marker was loaded in lane 1. (B) Total number of paired-reads obtained in each replicate experiment.

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi
    Article Snippet: Note: We recommend preparing MNase stock solution as follows: Dissolve MNase (Worthington) to 10 U/ml in 5 mM Na-phosphate pH 7.0, 0.025 mM CaCl2. .. 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.). ..

    Polymerase Chain Reaction:

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi
    Article Snippet: Note: We recommend preparing MNase stock solution as follows: Dissolve MNase (Worthington) to 10 U/ml in 5 mM Na-phosphate pH 7.0, 0.025 mM CaCl2. .. 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.). ..

    Marker:

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi
    Article Snippet: Note: We recommend preparing MNase stock solution as follows: Dissolve MNase (Worthington) to 10 U/ml in 5 mM Na-phosphate pH 7.0, 0.025 mM CaCl2. .. 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.). ..

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    worthington biochemical pcr dna marker
    Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. <t>PCR</t> <t>DNA</t> marker (NEB) was loaded in lane 10.
    Pcr Dna Marker, supplied by worthington biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr dna marker/product/worthington biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr dna marker - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Worthington Biochemical dna quantification
    Viability of <t>hMSCs</t> encapsulated in tethered TGF β hydrogels. <t>DNA</t> content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)
    Dna Quantification, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna quantification - by Bioz Stars, 2021-07
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    99
    Worthington Biochemical dnase i
    Analysis of <t>DNase</t> I hypersensitivity. HT-1080 (male human fibrosarcoma) cells and Δ3B (HT-1080 cells carrying the promoter mutation) cells were treated with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg of DNase I/ml. Southern blot analysis was performed on DNA digested with Eco RI and was hybridized to the HPRT promoter probe HPRT A. “HS site” indicates the positions of HPRT -hypersensitive sites; “globin” indicates the hybridization band detected by the control human β-globin probe.
    Dnase I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2021-07
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    85
    Worthington Biochemical e coli dna polymerase
    MALDI-TOF mass spectra of <t>SVPDE</t> digests of modified <t>DNA</t> 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.
    E Coli Dna Polymerase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dna polymerase/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli dna polymerase - by Bioz Stars, 2021-07
    85/100 stars
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    Image Search Results


    Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. PCR DNA marker (NEB) was loaded in lane 10.

    Journal: bioRxiv

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi

    doi: 10.1101/2021.07.02.450927

    Figure Lengend Snippet: Titration of T. cruzi chromatin with MNase. Chromatin digested with increasing amounts of MNase analyzed in a 2% agarose gel (lanes 1-9). An example of the level of digestion of choice is highlighted in lane 9. PCR DNA marker (NEB) was loaded in lane 10.

    Article Snippet: 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.).

    Techniques: Titration, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

    Mononucleosome sample and total number of paired-reads. (A) Chromatin digestions from the CL Brener replicate 1 experiment analyzed in a 2% agarose gel. The sample loaded in lane 4 was used for the experiment. PCR DNA (NEB) marker was loaded in lane 1. (B) Total number of paired-reads obtained in each replicate experiment.

    Journal: bioRxiv

    Article Title: Experimental and Bioinformatic upgrade for genome-wide mapping of nucleosomes in Trypanosoma cruzi

    doi: 10.1101/2021.07.02.450927

    Figure Lengend Snippet: Mononucleosome sample and total number of paired-reads. (A) Chromatin digestions from the CL Brener replicate 1 experiment analyzed in a 2% agarose gel. The sample loaded in lane 4 was used for the experiment. PCR DNA (NEB) marker was loaded in lane 1. (B) Total number of paired-reads obtained in each replicate experiment.

    Article Snippet: 3.4 Selecting the best mononucleosomal sample to proceed withTo determine the extent of digestion 10 μl of each MNase-digested sample was analyzed in a 2% agarose gel next to the PCR DNA Marker (New England Biolabs, Ipswich, Massachusetts, U.S.A.).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker

    Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

    Journal: Drug Delivery and Translational Research

    Article Title: Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells

    doi: 10.1007/s13346-012-0090-2

    Figure Lengend Snippet: Viability of hMSCs encapsulated in tethered TGF β hydrogels. DNA content of cell-laden hydrogels was assayed over 14 days of culture, and used as a general correlative measure of cell viability. Hydrogels cultured in growth medium (used as a negative control for chondrogenesis) maintained initial cell counts, as did those incorporating 1-nM tethered TGF β . Chondrogenic media samples (positive control) exhibited an increase in DNA of approximately twofold, while TGF β tethered at 10 or 100 nM demonstrated similar increases in DNA content, suggesting high levels of viability of the encapsulated hMSCs. Results are presented as mean ± s.e.m. ( n = 3)

    Article Snippet: DNA quantification of encapsulated hMSCs Immediately following photoencapsulation, cell-laden hydrogels (n = 3) were placed into an enzymatic digest buffer (125 μg/mL papain (Worthington Biochemical), 10 mM cysteine) overnight at 60 °C, then frozen prior to analysis.

    Techniques: Cell Culture, Negative Control, Positive Control

    Analysis of DNase I hypersensitivity. HT-1080 (male human fibrosarcoma) cells and Δ3B (HT-1080 cells carrying the promoter mutation) cells were treated with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg of DNase I/ml. Southern blot analysis was performed on DNA digested with Eco RI and was hybridized to the HPRT promoter probe HPRT A. “HS site” indicates the positions of HPRT -hypersensitive sites; “globin” indicates the hybridization band detected by the control human β-globin probe.

    Journal: Molecular and Cellular Biology

    Article Title: Role of the Promoter in Maintaining Transcriptionally Active Chromatin Structure and DNA Methylation Patterns In Vivo

    doi: 10.1128/MCB.23.12.4150-4161.2003

    Figure Lengend Snippet: Analysis of DNase I hypersensitivity. HT-1080 (male human fibrosarcoma) cells and Δ3B (HT-1080 cells carrying the promoter mutation) cells were treated with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg of DNase I/ml. Southern blot analysis was performed on DNA digested with Eco RI and was hybridized to the HPRT promoter probe HPRT A. “HS site” indicates the positions of HPRT -hypersensitive sites; “globin” indicates the hybridization band detected by the control human β-globin probe.

    Article Snippet: It is possible that the steeper slope detected by this probe is indicative of an intrinsically higher accessibility of the 5′flanking region to DNase I (or preferential DNase I cleavage of the underlying DNA sequence).

    Techniques: Mutagenesis, Southern Blot, Hybridization

    Physical map of the human  HPRT  gene. The thick horizontal line represents the  HPRT  gene. Open horizontal boxes represent exons, and “ATG” indicates the translation initiation site. The bent arrow represents the major transcription initiation sites, and a putative initiator element is shaded. The gray box indicates the position of probe “HPRT A.” The bracket above the line indicates the position of the 5′ flanking DNase I-hypersensitive site. The positions of CpG-dinucleotides within the gene are shown as vertical lines below the gene map. Numbers indicate the position of CpGs relative to the translation initiation site. The horizontal bracket below the line indicates the region deleted in Δ2B and Δ3B cells.

    Journal: Molecular and Cellular Biology

    Article Title: Role of the Promoter in Maintaining Transcriptionally Active Chromatin Structure and DNA Methylation Patterns In Vivo

    doi: 10.1128/MCB.23.12.4150-4161.2003

    Figure Lengend Snippet: Physical map of the human HPRT gene. The thick horizontal line represents the HPRT gene. Open horizontal boxes represent exons, and “ATG” indicates the translation initiation site. The bent arrow represents the major transcription initiation sites, and a putative initiator element is shaded. The gray box indicates the position of probe “HPRT A.” The bracket above the line indicates the position of the 5′ flanking DNase I-hypersensitive site. The positions of CpG-dinucleotides within the gene are shown as vertical lines below the gene map. Numbers indicate the position of CpGs relative to the translation initiation site. The horizontal bracket below the line indicates the region deleted in Δ2B and Δ3B cells.

    Article Snippet: It is possible that the steeper slope detected by this probe is indicative of an intrinsically higher accessibility of the 5′flanking region to DNase I (or preferential DNase I cleavage of the underlying DNA sequence).

    Techniques:

    MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Journal: Nucleic Acids Research

    Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine

    doi:

    Figure Lengend Snippet: MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Article Snippet: SVPDE and E.coli DNA polymerase I were purchased from Worthington Biochemicals (Lakewood, NJ).

    Techniques: Modification, Sequencing