dispase  (Worthington Biochemical)


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    • 96
    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    LS02100
    Price:
    70
    Source:
    Bacillus polymyxa
    Size:
    10 mg
    Cas Number:
    42613.33.2
    		Buy from Supplier

    Structured Review

    Worthington Biochemical dispase
    <t>Dispase</t> treatment reduces capsid redistribution to the nucleus. (A) pH-dependent dispase cleavage of AdV5 capsid hexon. Purified AdV5 capsids were treated with dispase at pH 7.4 or pH 4.4 or kept at pH 4.4 alone before being boiled in PSB and subjected
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-07
    96/100 stars

    Images

    1) Product Images from "Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment"

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment

    Journal: Journal of Virology

    doi: 10.1128/JVI.02889-14

    Dispase treatment reduces capsid redistribution to the nucleus. (A) pH-dependent dispase cleavage of AdV5 capsid hexon. Purified AdV5 capsids were treated with dispase at pH 7.4 or pH 4.4 or kept at pH 4.4 alone before being boiled in PSB and subjected
    Figure Legend Snippet: Dispase treatment reduces capsid redistribution to the nucleus. (A) pH-dependent dispase cleavage of AdV5 capsid hexon. Purified AdV5 capsids were treated with dispase at pH 7.4 or pH 4.4 or kept at pH 4.4 alone before being boiled in PSB and subjected

    Techniques Used: Purification

    Dispase hydrolysis inhibits low-pH effects on hexon structure and dynein binding. (A) Purified hexon was tested for dissociation after dispase treatment. Dispase exposure of hexon at low pH resulted in two major digestion products (85 kDa [I] and 15 kDa
    Figure Legend Snippet: Dispase hydrolysis inhibits low-pH effects on hexon structure and dynein binding. (A) Purified hexon was tested for dissociation after dispase treatment. Dispase exposure of hexon at low pH resulted in two major digestion products (85 kDa [I] and 15 kDa

    Techniques Used: Binding Assay, Purification

    The AdV5 hexon HVR1 mutant is inhibited in dynein binding and cytoplasmic transport. (A) Dispase insensitivity of mutant hexon. Immunopurified hexons of wild-type (WT) AdV5, AdV5HVR48(1), and WT AdV48 were tested for dissociation after dispase treatment.
    Figure Legend Snippet: The AdV5 hexon HVR1 mutant is inhibited in dynein binding and cytoplasmic transport. (A) Dispase insensitivity of mutant hexon. Immunopurified hexons of wild-type (WT) AdV5, AdV5HVR48(1), and WT AdV48 were tested for dissociation after dispase treatment.

    Techniques Used: Mutagenesis, Binding Assay

    Related Articles

    Isolation:

    Article Title: PDGFRα signalling promotes fibrogenic responses in collagen‐producing cells in Duchenne muscular dystrophy
    Article Snippet: .. Cells for molecular analysis were isolated by FACS from single cell preparations prepared by digesting a collection of limb muscles (TA, gastrocnemius, and quadriceps) with collagenase and dispase (Worthington Biochemical Corp, Lakewood, NJ, USA) , . .. RNA was isolated from cells or tissue as described previously .

    FACS:

    Article Title: PDGFRα signalling promotes fibrogenic responses in collagen‐producing cells in Duchenne muscular dystrophy
    Article Snippet: .. Cells for molecular analysis were isolated by FACS from single cell preparations prepared by digesting a collection of limb muscles (TA, gastrocnemius, and quadriceps) with collagenase and dispase (Worthington Biochemical Corp, Lakewood, NJ, USA) , . .. RNA was isolated from cells or tissue as described previously .

    Incubation:

    Article Title: A Transcriptomic Analysis of Differential Gene Expression during Chick Periocular Neural Crest Differentiation into Corneal Cells
    Article Snippet: .. All tissues were incubated in dispase (1.5 mg/ml; Worthington) at 38°C for 5 minutes, then rinsed twice in Ringer’s solution. ..

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: Briefly, immunoisolated hexon was incubated for 30 min in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20 [pH 4.4]), resuspended in the same buffer at pH 7.4 for the indicated amounts of time, and then exposed to purified rat brain cytoplasmic dynein at 4°C for 1.5 h. Hexon-bound dynein was assayed by immunoblotting. .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    Modification:

    Article Title: Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction
    Article Snippet: Rat MSC were isolated from subcutaneous adipose tissue. .. Briefly, the tissue sample was fragmented under sterile conditions to a homogeneous mass and subjected to enzymatic treatment in Dulbecco’s modified Eagle’s medium (DMEM) medium (Gibco, Waltham, MA, USA) with 10 U/mL dispase (Worthington Biochemical, Lakewood, NJ, USA) and 200 U/mL type I collagenase (Worthington Biochemical, Lakewood, NJ, USA) at 37 °C for 30 min. After centrifugation for 10 min at 200× g the cell pellet was resuspended in DMEM and filtered through 40 μm nylon membrane. .. Isolated cells were maintained in 4.5 g/L D-glucose DMEM with 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin solution at 37 °C and 5% CO2.

    Centrifugation:

    Article Title: Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction
    Article Snippet: Rat MSC were isolated from subcutaneous adipose tissue. .. Briefly, the tissue sample was fragmented under sterile conditions to a homogeneous mass and subjected to enzymatic treatment in Dulbecco’s modified Eagle’s medium (DMEM) medium (Gibco, Waltham, MA, USA) with 10 U/mL dispase (Worthington Biochemical, Lakewood, NJ, USA) and 200 U/mL type I collagenase (Worthington Biochemical, Lakewood, NJ, USA) at 37 °C for 30 min. After centrifugation for 10 min at 200× g the cell pellet was resuspended in DMEM and filtered through 40 μm nylon membrane. .. Isolated cells were maintained in 4.5 g/L D-glucose DMEM with 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin solution at 37 °C and 5% CO2.

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    • collagenase i  (Worthington Biochemical)
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    Worthington Biochemical collagenase i
    [A] Digestion of BAT with <t>collagenase</t> I produces an adipose fraction harboring brown adipocytes and a stromal fraction composed primarily of endothelium separated by a fluid portion. [B] Deep-red fluorescence intensity images of centrifugation tubes containing
    Collagenase I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase i - by Bioz Stars, 2021-07
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    dnase i  (Worthington Biochemical)
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    Worthington Biochemical dnase i
    <t>DNase</t> I knockdown can reduce the apoptosis of cells cultured with high glucose. (A) Knockdown efficiency examined by western blotting. Expression of DNase I, Bcl-2 and caspase-3 in the three groups were examined by (B) western blotting and (C) reverse transcription-quantitative polymerase chain reaction. (D) Apoptotic rate examined by flow cytometry [(a), normal; (b), high glucose; and (c), siRNA group]. Data are expressed as the mean ± standard deviation from three independent experiments. * P
    Dnase I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2021-07
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    Image Search Results


    [A] Digestion of BAT with collagenase I produces an adipose fraction harboring brown adipocytes and a stromal fraction composed primarily of endothelium separated by a fluid portion. [B] Deep-red fluorescence intensity images of centrifugation tubes containing

    Journal: Journal of materials chemistry. B, Materials for biology and medicine

    Article Title: Fluorescence Imaging of Interscapular Brown Adipose Tissue in Living Mice †

    doi: 10.1039/C4TB01914H

    Figure Lengend Snippet: [A] Digestion of BAT with collagenase I produces an adipose fraction harboring brown adipocytes and a stromal fraction composed primarily of endothelium separated by a fluid portion. [B] Deep-red fluorescence intensity images of centrifugation tubes containing

    Article Snippet: The minced tissue was digested with 2 mg/ml Collagenase I (Worthington Biochemical Corporation) in Hanks' Balanced Salt Solution (HBSS) (1.3 mM CaCl2 , 0.5 mM MgCl2 •6H2 O, 0.5 mM MgSO4 •7H2 O, 5.3 mM KCl, 0.40 mM KH2 PO4 , 4.2 mM NaHCO3 , 140 mM, NaCl, 0.3 mM Na2 HPO4 , pH 7.5) at 37 °C for 2 hours.

    Techniques: Fluorescence, Centrifugation

    DNase I knockdown can reduce the apoptosis of cells cultured with high glucose. (A) Knockdown efficiency examined by western blotting. Expression of DNase I, Bcl-2 and caspase-3 in the three groups were examined by (B) western blotting and (C) reverse transcription-quantitative polymerase chain reaction. (D) Apoptotic rate examined by flow cytometry [(a), normal; (b), high glucose; and (c), siRNA group]. Data are expressed as the mean ± standard deviation from three independent experiments. * P

    Journal: Molecular Medicine Reports

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes

    doi: 10.3892/mmr.2016.5102

    Figure Lengend Snippet: DNase I knockdown can reduce the apoptosis of cells cultured with high glucose. (A) Knockdown efficiency examined by western blotting. Expression of DNase I, Bcl-2 and caspase-3 in the three groups were examined by (B) western blotting and (C) reverse transcription-quantitative polymerase chain reaction. (D) Apoptotic rate examined by flow cytometry [(a), normal; (b), high glucose; and (c), siRNA group]. Data are expressed as the mean ± standard deviation from three independent experiments. * P

    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Techniques: Cell Culture, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Standard Deviation

    Immunohistochemistry of the human pancreas. Pancreatic tissues from patients with pancreatic cancer, with or without type 2 diabetes were stained with insulin, glucagon, and DNase I. (A) Insulin, (B) glucagon and (C) DNase I staining of tissues from patients without type 2 diabetes. (D) Insulin, (E) glucagon and (F) DNase I staining of tissues from patients with type 2 diabetes. DNase I, deoxyribonuclease I.

    Journal: Molecular Medicine Reports

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes

    doi: 10.3892/mmr.2016.5102

    Figure Lengend Snippet: Immunohistochemistry of the human pancreas. Pancreatic tissues from patients with pancreatic cancer, with or without type 2 diabetes were stained with insulin, glucagon, and DNase I. (A) Insulin, (B) glucagon and (C) DNase I staining of tissues from patients without type 2 diabetes. (D) Insulin, (E) glucagon and (F) DNase I staining of tissues from patients with type 2 diabetes. DNase I, deoxyribonuclease I.

    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Techniques: Immunohistochemistry, Staining

    DNase I combined with high glucose induced cell apoptosis. (A) Cell viability assessed by the Cell Counting Kit-8 assay. The expression levels of DNase I, Bcl-2 and caspase-3 in the three groups were examined by (B) western blotting and (C) reverse transcription-quantitative polymerase chain reaction. (D) Cell apoptosis results from (a) the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay and (b) flow cytometry. Data are expressed as the mean ± standard deviation from three independent experiments. * P

    Journal: Molecular Medicine Reports

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes

    doi: 10.3892/mmr.2016.5102

    Figure Lengend Snippet: DNase I combined with high glucose induced cell apoptosis. (A) Cell viability assessed by the Cell Counting Kit-8 assay. The expression levels of DNase I, Bcl-2 and caspase-3 in the three groups were examined by (B) western blotting and (C) reverse transcription-quantitative polymerase chain reaction. (D) Cell apoptosis results from (a) the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay and (b) flow cytometry. Data are expressed as the mean ± standard deviation from three independent experiments. * P

    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Techniques: Cell Counting, Expressing, Western Blot, Real-time Polymerase Chain Reaction, TUNEL Assay, Flow Cytometry, Standard Deviation

    DNase I activity in human serum. (A) DNase I activity in human serum. (B) The correlation of DNase I activity with calcium. (C) DNase I activity Spearman correlation coefficient. All data are presented as the mean ± standard deviation. *** P

    Journal: Molecular Medicine Reports

    Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes

    doi: 10.3892/mmr.2016.5102

    Figure Lengend Snippet: DNase I activity in human serum. (A) DNase I activity in human serum. (B) The correlation of DNase I activity with calcium. (C) DNase I activity Spearman correlation coefficient. All data are presented as the mean ± standard deviation. *** P

    Article Snippet: DNase I is ubiquitously expressed in mammalian tissues, particularly in the pancreas ( ).

    Techniques: Activity Assay, Standard Deviation

    Effects of HIV-1 IN mutations on viral infectivity. Viruses were prepared by cotransfection of COS-7 cells with the pNL43lucΔenv vector containing either WT IN or mutant IN together with an amphotropic Moloney MuLV envelope expression vector (pJD-1) or a macrophage-tropic HIV-1 envelope vector (pJR-FL) by using Lipofectamine. At 48 h posttransfection, culture supernatants of the transfected COS-7 cells were harvested. DNase I-treated supernatants were inoculated into 10 5 RD cells, PBLs, and MDMs. At 4 days postinfection, the cells were washed with PBS and lysed with 200 μl of cell lysis buffer. Ten microliters of each cell lysate was subjected to the luciferase assay. Mean values from five independent experiments are shown with the error bars.

    Journal: Journal of Virology

    Article Title: Evaluation of the Functional Involvement of Human Immunodeficiency Virus Type 1 Integrase in Nuclear Import of Viral cDNA during Acute Infection

    doi: 10.1128/JVI.78.21.11563-11573.2004

    Figure Lengend Snippet: Effects of HIV-1 IN mutations on viral infectivity. Viruses were prepared by cotransfection of COS-7 cells with the pNL43lucΔenv vector containing either WT IN or mutant IN together with an amphotropic Moloney MuLV envelope expression vector (pJD-1) or a macrophage-tropic HIV-1 envelope vector (pJR-FL) by using Lipofectamine. At 48 h posttransfection, culture supernatants of the transfected COS-7 cells were harvested. DNase I-treated supernatants were inoculated into 10 5 RD cells, PBLs, and MDMs. At 4 days postinfection, the cells were washed with PBS and lysed with 200 μl of cell lysis buffer. Ten microliters of each cell lysate was subjected to the luciferase assay. Mean values from five independent experiments are shown with the error bars.

    Article Snippet: After treatment with DNase I (40 μg/ml; Worthington), each virus (70 ng of p24) was inoculated into HeLa cells and cultured at 37°C for 6 h. The cells were washed with PBS and resuspended in fresh medium (DMEM plus 10% FBS).

    Techniques: Infection, Cotransfection, Plasmid Preparation, Mutagenesis, Expressing, Transfection, Lysis, Luciferase