dispase  (Worthington Biochemical)


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    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    ls02100
    Price:
    70
    Size:
    10 mg
    Source:
    Bacillus polymyxa
    Cas Number:
    42613.33.2
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    Structured Review

    Worthington Biochemical dispase
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 97 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: ADAM17 Deletion in Thymic Epithelial Cells Alters Aire Expression without Affecting T Cell Developmental Progression
    Article Snippet: .. After 4 digestions, 0.125% (w/v) dispase (Worthington Biochemical) was added to the digestion buffer and thymi were incubated for an additional 15 minutes. .. After the final digestion, cells were incubated in PBS containing 5 mM EDTA + 1% FCS + 0.02% (w/v) NaN3 for 10 minutes on ice.

    Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration
    Article Snippet: .. Next, the specimen was incubated in 8 U/ml Dispase (Worthington Biochemical, Lakewood, NJ, http://www.worthington-biochem.com ) at 4°C overnight. .. The dermis was separated from the epidermis, cut into small pieces, and further digested in 2 mg/ml collagenase (NB4 [PZ activity, 0.170 U/mg]; SERVA Electrophoresis, Germany, http://www.serva.de ), which was diluted in Dulbecco's modified Eagle's medium–low glucose (DMEM‐lg) (Thermo Fisher Scientific Life Sciences, Waltham, MA, http://www.thermofisher.com ) at 37°C for 3 hours in a shaking water bath.

    Article Title: High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor
    Article Snippet: .. Single-cell RNA-seq Cochleae were dissected out and incubated in solution (50% accutase (Innovative Cell Technologies, San Diego), 0.02% Trypsin(Thermofisher), 125 μg/ml Thermolysin (SIGMA-ALDRICH)) at 37°C for 3 min. 0.02 mg/ml collagenase IV (SIGMA-ALDRICH) and dispase (Worthington Biochemicals Corp) were further added to the enzyme solution and incubated for 4 min at 37°C. ..

    Article Title: Homology‐guided mutational analysis reveals the functional requirements for antinociceptive specificity of collapsin response mediator protein 2‐derived peptides
    Article Snippet: .. DRGs were then collected, trimmed at their roots and digested in 3 mL bicarbonate‐free, serum‐free, sterile DMEM (Cat#11965; Thermo Fisher Scientific) solution containing neutral protease (3.125 mg·mL−1 , Cat#LS02104, Worthington, Lakewood, NJ, USA) and collagenase Type I (5 mg·mL−1 , Cat# , Worthington) and incubated for 60 min at 37°C under gentle agitation. .. Dissociated DRG neurons (~1.5 × 106 ) were then gently centrifuged to collect cells and washed with DRG media DMEM containing 1% penicillin/streptomycin sulfate from 10 000 μg·mL−1 stock, 30 ng·mL−1 nerve growth factor and 10% fetal bovine serum (Hyclone) before plating onto poly‐ d ‐lysine‐ and laminin‐coated glass 12 or 15 mm coverslips.

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    RNA Sequencing Assay:

    Article Title: High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor
    Article Snippet: .. Single-cell RNA-seq Cochleae were dissected out and incubated in solution (50% accutase (Innovative Cell Technologies, San Diego), 0.02% Trypsin(Thermofisher), 125 μg/ml Thermolysin (SIGMA-ALDRICH)) at 37°C for 3 min. 0.02 mg/ml collagenase IV (SIGMA-ALDRICH) and dispase (Worthington Biochemicals Corp) were further added to the enzyme solution and incubated for 4 min at 37°C. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Homology‐guided mutational analysis reveals the functional requirements for antinociceptive specificity of collapsin response mediator protein 2‐derived peptides
    Article Snippet: .. DRGs were then collected, trimmed at their roots and digested in 3 mL bicarbonate‐free, serum‐free, sterile DMEM (Cat11965; Thermo Fisher Scientific) solution containing neutral protease (3.125 mg·mL−1 , Cat#LS02104, Worthington, Lakewood, NJ, USA) and collagenase Type I (5 mg·mL−1 , Cat# , Worthington) and incubated for 60 min at 37°C under gentle agitation. .. Dissociated DRG neurons (~1.5 × 106 ) were then gently centrifuged to collect cells and washed with DRG media DMEM containing 1% penicillin/streptomycin sulfate from 10 000 μg·mL−1 stock, 30 ng·mL−1 nerve growth factor and 10% fetal bovine serum (Hyclone) before plating onto poly‐ d ‐lysine‐ and laminin‐coated glass 12 or 15 mm coverslips.

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  • 97
    Worthington Biochemical dispase
    Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of <t>dispase</t> pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.
    Dispase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 97/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 97 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

    99
    Worthington Biochemical collagenase type 2
    Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and <t>type</t> 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (
    Collagenase Type 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
    Average 99 stars, based on 445 article reviews
    Price from $9.99 to $1999.99
    collagenase type 2 - by Bioz Stars, 2021-01
    99/100 stars
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    99
    Worthington Biochemical tpck trypsin
    Separation by size-exclusion chromatography of tryptic peptides derived from HEWL labeled with methylene carbene. After the clean-up step described above (see Fig. ), HEWL samples were reduced, <t>carbamidomethylated,</t> and digested with <t>TPCK-trypsin</t>
    Tpck Trypsin, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tpck trypsin/product/Worthington Biochemical
    Average 99 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    tpck trypsin - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of dispase pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.

    Journal: Acta physiologica (Oxford, England)

    Article Title: Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving integrity.

    doi: 10.1111/apha.13182

    Figure Lengend Snippet: Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of dispase pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.

    Article Snippet: Reagents: Dispase (neutral protease) was obtained from Worthington Biochemical Corporation (Lakewood, NJ, USA).

    Techniques: Homogenization, Centrifugation

    Two distinct fragments of a cell found within the skeletal muscle pellet treated with dispase protease (Scale line = 2μm) in panel (a) and (b). The sarcolemma, subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria are identified.

    Journal: Acta physiologica (Oxford, England)

    Article Title: Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving integrity.

    doi: 10.1111/apha.13182

    Figure Lengend Snippet: Two distinct fragments of a cell found within the skeletal muscle pellet treated with dispase protease (Scale line = 2μm) in panel (a) and (b). The sarcolemma, subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria are identified.

    Article Snippet: Reagents: Dispase (neutral protease) was obtained from Worthington Biochemical Corporation (Lakewood, NJ, USA).

    Techniques:

    Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: SMAD3 Deficiency Promotes Inflammatory Aortic Aneurysms in Angiotensin II-Infused Mice Via Activation of iNOS

    doi: 10.1161/JAHA.113.000269

    Figure Lengend Snippet: Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

    Article Snippet: Briefly, the whole aorta was dissected out from its origin at the left ventricle to the iliac bifurcation, denuded of periaortic fat, cut into small pieces (≈2‐mm segments), and incubated in 5 mL of Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (#16000036; Life Technologies) and 1.36 mg/mL of type II collagenase (#LS004174; Worthington Biochemical Corporation) in agitation at 115 rpm for 2 hours at 37°C.

    Techniques: Concentration Assay, Construct, Incubation, Derivative Assay, Generated, Mouse Assay, Expressing, Ex Vivo

    The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

    Journal: Life sciences

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart

    doi: 10.1016/j.lfs.2012.06.011

    Figure Lengend Snippet: The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

    Article Snippet: Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington).

    Techniques: Western Blot

    The temporal progression of body weight (A) and survival (B) following either sham or trans-aortic constriction surgery to induce a pressure overload (PO) in non-diabetic (control), high fat-fed, or high fat-fed and STZ treated type 2 diabetic rats. †

    Journal: Life sciences

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart

    doi: 10.1016/j.lfs.2012.06.011

    Figure Lengend Snippet: The temporal progression of body weight (A) and survival (B) following either sham or trans-aortic constriction surgery to induce a pressure overload (PO) in non-diabetic (control), high fat-fed, or high fat-fed and STZ treated type 2 diabetic rats. †

    Article Snippet: Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington).

    Techniques:

    Separation by size-exclusion chromatography of tryptic peptides derived from HEWL labeled with methylene carbene. After the clean-up step described above (see Fig. ), HEWL samples were reduced, carbamidomethylated, and digested with TPCK-trypsin

    Journal:

    Article Title: Exploring protein interfaces with a general photochemical reagent

    doi: 10.1110/ps.051960406

    Figure Lengend Snippet: Separation by size-exclusion chromatography of tryptic peptides derived from HEWL labeled with methylene carbene. After the clean-up step described above (see Fig. ), HEWL samples were reduced, carbamidomethylated, and digested with TPCK-trypsin

    Article Snippet: Complete enzymatic digestion of these carbamidomethylated samples with TPCK trypsin was achieved in NH4 CO3 H (0.1 M at pH 8.0), after 12–24 h at 37°C using a 2% (w/w) enzyme:substrate ratio.

    Techniques: Size-exclusion Chromatography, Derivative Assay, Labeling