dispase  (Worthington Biochemical)


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  • 99
    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    ls02100
    Price:
    70
    Size:
    10 mg
    Source:
    Bacillus polymyxa
    Cas Number:
    42613.33.2
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    Structured Review

    Worthington Biochemical dispase
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor
    Article Snippet: .. Single-cell RNA-seq Cochleae were dissected out and incubated in solution (50% accutase (Innovative Cell Technologies, San Diego), 0.02% Trypsin(Thermofisher), 125 μg/ml Thermolysin (SIGMA-ALDRICH)) at 37°C for 3 min. 0.02 mg/ml collagenase IV (SIGMA-ALDRICH) and dispase (Worthington Biochemicals Corp) were further added to the enzyme solution and incubated for 4 min at 37°C. ..

    Fluorescence:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Isolation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Mouse Assay:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Incubation:

    Article Title: ADAM17 Deletion in Thymic Epithelial Cells Alters Aire Expression without Affecting T Cell Developmental Progression
    Article Snippet: .. After 4 digestions, 0.125% (w/v) dispase (Worthington Biochemical) was added to the digestion buffer and thymi were incubated for an additional 15 minutes. .. After the final digestion, cells were incubated in PBS containing 5 mM EDTA + 1% FCS + 0.02% (w/v) NaN3 for 10 minutes on ice.

    Article Title: The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells
    Article Snippet: .. Cochlear tissue (organ of Corti and spiral ganglion) was dissected then incubated in an enzyme solution consisting of 50% Accutase (Innovative Cell Technologies, San Diego, CA), 0.02% trypsin (Thermo Fisher Scientific, Waltham, MA), and 125 µg/mL thermolysin (Sigma-Aldrich, St. Louis, MO) in Hank’s balanced salt solution (HBSS) at 37 °C for 3 min. Then, 0.02 mg/mL collagenase IV (Sigma-Aldrich) and dispase (Worthington Biochemical Corporation, Lakewood, NJ) were added to the enzyme solution and the tissue was incubated for a further 4 min at 37 °C. .. The tissue was then disrupted by trituration with a fire- polished glass Pasteur pipette.

    Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration
    Article Snippet: .. Next, the specimen was incubated in 8 U/ml Dispase (Worthington Biochemical, Lakewood, NJ, http://www.worthington-biochem.com ) at 4°C overnight. .. The dermis was separated from the epidermis, cut into small pieces, and further digested in 2 mg/ml collagenase (NB4 [PZ activity, 0.170 U/mg]; SERVA Electrophoresis, Germany, http://www.serva.de ), which was diluted in Dulbecco's modified Eagle's medium–low glucose (DMEM‐lg) (Thermo Fisher Scientific Life Sciences, Waltham, MA, http://www.thermofisher.com ) at 37°C for 3 hours in a shaking water bath.

    Article Title: High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor
    Article Snippet: .. Single-cell RNA-seq Cochleae were dissected out and incubated in solution (50% accutase (Innovative Cell Technologies, San Diego), 0.02% Trypsin(Thermofisher), 125 μg/ml Thermolysin (SIGMA-ALDRICH)) at 37°C for 3 min. 0.02 mg/ml collagenase IV (SIGMA-ALDRICH) and dispase (Worthington Biochemicals Corp) were further added to the enzyme solution and incubated for 4 min at 37°C. ..

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    FACS:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Magnetic Cell Separation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

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  • 99
    Worthington Biochemical neonatal cardiomyocytes isolation system
    Heterogeneity of our isolated CFs (Thy1+ non-immune non-myocyte cardiac cells) and stepwise suppression of <t>non-cardiomyocyte</t> lineages during iCM reprogramming Related to . ( a-c ) Limited transcriptome change by retrovirus transduction. To determine whether introduction of viruses could influence cellular identities of CF, molecular features of the uninfected and DsRed-transduced cells were compared and only 25 genes were differentially expressed (ANOVA p value
    Neonatal Cardiomyocytes Isolation System, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neonatal cardiomyocytes isolation system/product/Worthington Biochemical
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    neonatal cardiomyocytes isolation system - by Bioz Stars, 2020-10
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    95
    Worthington Biochemical proteinase k
    Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized <t>proteinase</t> K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).
    Proteinase K, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Worthington Biochemical
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    94
    Worthington Biochemical v8 protease
    Proteolytic cleavage of Smc3 wild-type and mutant proteins. Smc3-V5 and smc3-L217P-3V5 were pulled down by using antibodies against V5. The precipitated proteins were washed and incubated with <t>V8</t> protease at 23°C in the absence ( A ) or presence ( B ) of 1 mM ATP. The reactions were stopped at the indicated time points. The cleaved products were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and analyzed by western blot, using antibody against V5. The major cleaved products discussed in the text are labeled as a–e. * indicates a non-specific band. A representative experiment is shown. ( n = 4).
    V8 Protease, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v8 protease/product/Worthington Biochemical
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    99
    Worthington Biochemical mix collagenase type 1
    Attachment and morphology of cultured human fungiform taste cells. ( a ) Primary cell cultures grown on collagen <t>type-1-coated</t> plates were imaged after 2 days. ( b ) Cells from human fungiform papillae grew for up to 2–4 weeks under attached cell clusters. ( c and d ) Represent day 2 and 4 weeks after harvesting, respectively. During this period we did not observe growth of cells with the appearance of non-taste epithelial cells.
    Mix Collagenase Type 1, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Heterogeneity of our isolated CFs (Thy1+ non-immune non-myocyte cardiac cells) and stepwise suppression of non-cardiomyocyte lineages during iCM reprogramming Related to . ( a-c ) Limited transcriptome change by retrovirus transduction. To determine whether introduction of viruses could influence cellular identities of CF, molecular features of the uninfected and DsRed-transduced cells were compared and only 25 genes were differentially expressed (ANOVA p value

    Journal: Nature

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte

    doi: 10.1038/nature24454

    Figure Lengend Snippet: Heterogeneity of our isolated CFs (Thy1+ non-immune non-myocyte cardiac cells) and stepwise suppression of non-cardiomyocyte lineages during iCM reprogramming Related to . ( a-c ) Limited transcriptome change by retrovirus transduction. To determine whether introduction of viruses could influence cellular identities of CF, molecular features of the uninfected and DsRed-transduced cells were compared and only 25 genes were differentially expressed (ANOVA p value

    Article Snippet: Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability.

    Techniques: Isolation, Transduction

    Heterogeneity of CF and stepwise suppression of non-cardiomyocyte lineages during iCM induction ( a-b ) HC (a) and PCA (b) of control CFs with representative gene expression and GO analysis of the five identified gene clusters. ( c ) HC calculated with control CFs (a) applied to M+G+T-transduced cells with representative gene expression. ( d-f ) 40× ICC images (d,e) with quantifications (f) of Thy1 and SM22α co-stained with αMHC-GFP during reprogramming. n=20 images, scale bar=100 μm, error bars indicate SEM.

    Journal: Nature

    Article Title: Single Cell Transcriptomics Reconstructs Fate Conversion from Fibroblast to Cardiomyocyte

    doi: 10.1038/nature24454

    Figure Lengend Snippet: Heterogeneity of CF and stepwise suppression of non-cardiomyocyte lineages during iCM induction ( a-b ) HC (a) and PCA (b) of control CFs with representative gene expression and GO analysis of the five identified gene clusters. ( c ) HC calculated with control CFs (a) applied to M+G+T-transduced cells with representative gene expression. ( d-f ) 40× ICC images (d,e) with quantifications (f) of Thy1 and SM22α co-stained with αMHC-GFP during reprogramming. n=20 images, scale bar=100 μm, error bars indicate SEM.

    Article Snippet: Neonatal CMs were isolated using the neonatal cardiomyocytes isolation system (Worthington Biochemical Corporation) except that all enzymes were used at a ¼ of the recommended concentration to increase cell viability.

    Techniques: Expressing, Immunocytochemistry, Staining

    Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized proteinase K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).

    Journal: Journal of Synchrotron Radiation

    Article Title: High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner

    doi: 10.1107/S1600577518010354

    Figure Lengend Snippet: Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized proteinase K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).

    Article Snippet: Proteinase K crystals were grown on siliconized glass cover slips (hanging drop) by equilibrating 25 mg ml−1 proteinase K (Worthington Biochemical; LS004224) in 40 mM calcium chloride, 400 mM sodium nitrate and 50 mM BisTris (pH 6.5) over a reservoir containing 160 mM calcium chloride, 1.6 M sodium nitrate and 200 mM BisTris (pH 6.5).

    Techniques:

    Proteolytic cleavage of Smc3 wild-type and mutant proteins. Smc3-V5 and smc3-L217P-3V5 were pulled down by using antibodies against V5. The precipitated proteins were washed and incubated with V8 protease at 23°C in the absence ( A ) or presence ( B ) of 1 mM ATP. The reactions were stopped at the indicated time points. The cleaved products were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and analyzed by western blot, using antibody against V5. The major cleaved products discussed in the text are labeled as a–e. * indicates a non-specific band. A representative experiment is shown. ( n = 4).

    Journal: Nucleic Acids Research

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity

    doi: 10.1093/nar/gkw539

    Figure Lengend Snippet: Proteolytic cleavage of Smc3 wild-type and mutant proteins. Smc3-V5 and smc3-L217P-3V5 were pulled down by using antibodies against V5. The precipitated proteins were washed and incubated with V8 protease at 23°C in the absence ( A ) or presence ( B ) of 1 mM ATP. The reactions were stopped at the indicated time points. The cleaved products were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and analyzed by western blot, using antibody against V5. The major cleaved products discussed in the text are labeled as a–e. * indicates a non-specific band. A representative experiment is shown. ( n = 4).

    Article Snippet: Smc3-3V5 or smc3-L217P-3V5 were precipitated from asynchronous cell cultures and incubated with V8 protease, with or without ATP.

    Techniques: Mutagenesis, Incubation, Polyacrylamide Gel Electrophoresis, Western Blot, Labeling

    Attachment and morphology of cultured human fungiform taste cells. ( a ) Primary cell cultures grown on collagen type-1-coated plates were imaged after 2 days. ( b ) Cells from human fungiform papillae grew for up to 2–4 weeks under attached cell clusters. ( c and d ) Represent day 2 and 4 weeks after harvesting, respectively. During this period we did not observe growth of cells with the appearance of non-taste epithelial cells.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Primary Culture of Mammalian Taste Epithelium

    doi: 10.1007/978-1-62703-125-7_7

    Figure Lengend Snippet: Attachment and morphology of cultured human fungiform taste cells. ( a ) Primary cell cultures grown on collagen type-1-coated plates were imaged after 2 days. ( b ) Cells from human fungiform papillae grew for up to 2–4 weeks under attached cell clusters. ( c and d ) Represent day 2 and 4 weeks after harvesting, respectively. During this period we did not observe growth of cells with the appearance of non-taste epithelial cells.

    Article Snippet: Enzyme mixture: Mix collagenase type 1 (550 U/mL, Worthington), elastase (10 U/mL, Worthington), and soy bean trypsin inhibitor (0.9 mg/mL, Worthington) in calcium-free Ringer solution just before use.

    Techniques: Cell Culture