dispase  (Worthington Biochemical)


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  • 97
    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    ls02100
    Price:
    70
    Size:
    10 mg
    Source:
    Bacillus polymyxa
    Cas Number:
    42613.33.2
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    Structured Review

    Worthington Biochemical dispase
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 97 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2020-05
    97/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Isolation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Purification:

    Article Title: Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
    Article Snippet: .. Adipose tissue digestion and SVF preparation Posterior subcutaneous adipose tissue (gluteal + inguinal) or the brown part of the interscapular fat was dissected, minced with scissors, and digested with 0.1 w.u./ml purified collagenase (LS005273, Worthington Biochemical, Lakewood, NJ, USA) and 2.4 U/ml Neutral Protease (LS02104, Worthington Biochemical) in Hank’s balanced salt solution (HBSS, Sigma-Aldrich, Munich, Germany) containing 4 mM calcium chloride and 0.05 mg/ml DNase I (1284932001, Roche Diagnostics, Grenzach-Wyhlen, Germany) for 50 min at 37°C in a shaker. ..

    Incubation:

    Article Title: CHARACTERIZING CALCIUM INFLUX VIA VOLTAGE- AND LIGAND-GATED CALCIUM CHANNELS IN EMBRYONIC ALLIGATOR NEURONS IN CULTURE
    Article Snippet: .. The brains were then transferred to a dissociation enzyme cocktail containing 1 µg/ml dispase (a mettalo, neutral protease; Worthington Biochemical Corporation, Lakewood, NJ) and 1.67 µg/ml collagenase (Worthington Biochemical Corporation) in ACSF and incubated for 10 min at 37°C. .. Then, the brain/dissociation enzyme cocktail was gently mechanically sheared by pipetting it through a 200 µl Eppendorf pipette at least 3 times and returned to 37°C for another 15 min.

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    Article Title: (S)-Lacosamide inhibition of CRMP2 phosphorylation reduces postoperative and neuropathic pain behaviors through distinct classes of sensory neurons identified by constellation pharmacology
    Article Snippet: .. The ganglia were dissociated enzymatically by a 45 min incubation (37°C) in a DMEM (Cat# 11965, Life technologies) solution containing neutral protease (3.125 mg.ml−1 , Cat#LS02104, Worthington) and collagenase Type I (5 mg.ml−1 , Cat# LS004194, Worthington). .. The dissociated cells were resuspended in complete DRG medium, DMEM containing penicillin (100 U/mL), streptomycin (100 µg/mL), 30 ng.ml−1 nerve growth factor and 10% fetal bovine serum (Hyclone).

    FACS:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: (S)-Lacosamide inhibition of CRMP2 phosphorylation reduces postoperative and neuropathic pain behaviors through distinct classes of sensory neurons identified by constellation pharmacology
    Article Snippet: .. The ganglia were dissociated enzymatically by a 45 min incubation (37°C) in a DMEM (Cat# 11965, Life technologies) solution containing neutral protease (3.125 mg.ml−1 , CatLS02104, Worthington) and collagenase Type I (5 mg.ml−1 , Cat# LS004194, Worthington). .. The dissociated cells were resuspended in complete DRG medium, DMEM containing penicillin (100 U/mL), streptomycin (100 µg/mL), 30 ng.ml−1 nerve growth factor and 10% fetal bovine serum (Hyclone).

    Mouse Assay:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Magnetic Cell Separation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.