dispase  (Worthington Biochemical)


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  • 95
    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    ls02100
    Price:
    70
    Source:
    Bacillus polymyxa
    Size:
    10 mg
    Cas Number:
    42613.33.2
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    Structured Review

    Worthington Biochemical dispase
    Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of <t>dispase</t> pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/dispase/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-03
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    Images

    1) Product Images from "Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving integrity."

    Article Title: Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving integrity.

    Journal: Acta physiologica (Oxford, England)

    doi: 10.1111/apha.13182

    Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of dispase pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.
    Figure Legend Snippet: Two distinct cell fragments found within a myofibrillar pellet after homogenization and centrifugation of dispase pellet (Scale line = 2μm) in panel (a) and (b). The sarcolemma and interfibrillar (IFM) mitochondria are identified.

    Techniques Used: Homogenization, Centrifugation

    Two distinct fragments of a cell found within the skeletal muscle pellet treated with dispase protease (Scale line = 2μm) in panel (a) and (b). The sarcolemma, subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria are identified.
    Figure Legend Snippet: Two distinct fragments of a cell found within the skeletal muscle pellet treated with dispase protease (Scale line = 2μm) in panel (a) and (b). The sarcolemma, subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria are identified.

    Techniques Used:

    Related Articles

    Microscopy:

    Article Title: Delivery of Proteases in Aqueous Two-Phase Systems Enables Direct Purification of Stem Cell Colonies from Feeder Cell Co-Cultures for Differentiation into Functional Cardiomyocytes
    Article Snippet: .. Cells were immediately transferred to the microscope with the pneumatic ejection system and a capillary loaded with a solution of DMEM/F12 containing 16% DEX 10,000 kDa, 0.2 mg/mL collagenase (Worthington, 280 U/mg) and 0.2 mg/mL dispase (Worthington, 1.46 U/mg). ..

    Incubation:

    Article Title: CHARACTERIZING CALCIUM INFLUX VIA VOLTAGE- AND LIGAND-GATED CALCIUM CHANNELS IN EMBRYONIC ALLIGATOR NEURONS IN CULTURE
    Article Snippet: .. The brains were then transferred to a dissociation enzyme cocktail containing 1 µg/ml dispase (a mettalo, neutral protease; Worthington Biochemical Corporation, Lakewood, NJ) and 1.67 µg/ml collagenase (Worthington Biochemical Corporation) in ACSF and incubated for 10 min at 37°C. .. Then, the brain/dissociation enzyme cocktail was gently mechanically sheared by pipetting it through a 200 µl Eppendorf pipette at least 3 times and returned to 37°C for another 15 min.

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: Briefly, immunoisolated hexon was incubated for 30 min in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20 [pH 4.4]), resuspended in the same buffer at pH 7.4 for the indicated amounts of time, and then exposed to purified rat brain cytoplasmic dynein at 4°C for 1.5 h. Hexon-bound dynein was assayed by immunoblotting. .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    Isolation:

    Article Title: Immortalized Mouse Floxed Bmp2 Dental Papilla Mesenchymal Cell Lines Preserve Odontoblastic Phenotype and Respond to BMP2
    Article Snippet: .. The dental papilla mesenchymal cells were isolated from the first molars of 1-day-old floxed Bmp2 mice and washed with phosphate-buffered saline (PBS), digested for 1 h at 37°C in a solution of 3 mg/ml collagenase type I and 4 mg/ml of dispase (Worthington Biochem, Freehold, NJ). .. The cells were grown with alpha minimum essential medium (α-MEM, Invitrogen, San Diego, CA) containing 10% fetal calf serum plus penicillin (100 U/ml) and streptomycin (100 μg/ml), and cultured at 37°C in a humidified atmosphere of air containing 5% CO2 .

    Mouse Assay:

    Article Title: Immortalized Mouse Floxed Bmp2 Dental Papilla Mesenchymal Cell Lines Preserve Odontoblastic Phenotype and Respond to BMP2
    Article Snippet: .. The dental papilla mesenchymal cells were isolated from the first molars of 1-day-old floxed Bmp2 mice and washed with phosphate-buffered saline (PBS), digested for 1 h at 37°C in a solution of 3 mg/ml collagenase type I and 4 mg/ml of dispase (Worthington Biochem, Freehold, NJ). .. The cells were grown with alpha minimum essential medium (α-MEM, Invitrogen, San Diego, CA) containing 10% fetal calf serum plus penicillin (100 U/ml) and streptomycin (100 μg/ml), and cultured at 37°C in a humidified atmosphere of air containing 5% CO2 .

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    Worthington Biochemical low endotoxin ova
    PD-L2 expression is enhanced in the airways of asthmatic individuals and allergen-exposed mice Mice were treated with PBS (PBS - intratracheally on days 0, and 14), intratracheal <t>OVA</t> (OVA i.t. - 100 μg on days 0, 14 and 21), intraperitoneal/intratracheal OVA (OVA i.p./i.t. - 10 μg OVA i.p. on day 0 followed by 100 μg i.t. on days 14 and 21), or intratracheal HDM (HDM - 100 μg on days 0 and 14). (A) Mice were sacrificed 72 hours after final allergen exposure to measure <t>AHR</t> via the airway pressure time index (APTI) method. (B) PD-L2 expression (normalized to mouse ribosomal protein s14) was measured by RT-PCR. n = 4 mice per group. 1 representative experiment of 2 shown. Mean + SEM shown. *** and ** indicate p
    Low Endotoxin Ova, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Worthington Biochemical proteinase k
    Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized <t>proteinase</t> K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).
    Proteinase K, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Worthington Biochemical pepsin a
    (a) Number of <t>pepsin,</t> trypsin and chymotrypsin cleavage sites for 16-aa fragments of shrimp Tpm starting every six residues (i.e. 1–16, 7–22 …). Pink bars indicate fragments that overlap epitopes. (b) Epitopes (red) and a or d heptad position Ala-clusters (yellow) highlighted on a model of the Tpm coiled coil. (c) interhelical spacing is calculated between the d position on one chain and the a and a’ postions on the neighboring chain as ( da + da’ )/2. (d) mean interhelical spacing over the shrimp Tpm molecular dyanmics trajectory at acid and neutral pH. (e) .
    Pepsin A, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Worthington Biochemical e coli dna polymerase
    MALDI-TOF mass spectra of <t>SVPDE</t> digests of modified <t>DNA</t> 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.
    E Coli Dna Polymerase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PD-L2 expression is enhanced in the airways of asthmatic individuals and allergen-exposed mice Mice were treated with PBS (PBS - intratracheally on days 0, and 14), intratracheal OVA (OVA i.t. - 100 μg on days 0, 14 and 21), intraperitoneal/intratracheal OVA (OVA i.p./i.t. - 10 μg OVA i.p. on day 0 followed by 100 μg i.t. on days 14 and 21), or intratracheal HDM (HDM - 100 μg on days 0 and 14). (A) Mice were sacrificed 72 hours after final allergen exposure to measure AHR via the airway pressure time index (APTI) method. (B) PD-L2 expression (normalized to mouse ribosomal protein s14) was measured by RT-PCR. n = 4 mice per group. 1 representative experiment of 2 shown. Mean + SEM shown. *** and ** indicate p

    Journal: Mucosal immunology

    Article Title: PD-L2 modulates asthma severity by directly decreasing dendritic cell IL-12 production

    doi: 10.1038/mi.2012.111

    Figure Lengend Snippet: PD-L2 expression is enhanced in the airways of asthmatic individuals and allergen-exposed mice Mice were treated with PBS (PBS - intratracheally on days 0, and 14), intratracheal OVA (OVA i.t. - 100 μg on days 0, 14 and 21), intraperitoneal/intratracheal OVA (OVA i.p./i.t. - 10 μg OVA i.p. on day 0 followed by 100 μg i.t. on days 14 and 21), or intratracheal HDM (HDM - 100 μg on days 0 and 14). (A) Mice were sacrificed 72 hours after final allergen exposure to measure AHR via the airway pressure time index (APTI) method. (B) PD-L2 expression (normalized to mouse ribosomal protein s14) was measured by RT-PCR. n = 4 mice per group. 1 representative experiment of 2 shown. Mean + SEM shown. *** and ** indicate p

    Article Snippet: Where indicated mice were treated with low-endotoxin OVA (Worthington Biochemicals, Lakewood, NJ) to induce AHR (10 μg i.p. on day 0, and 100 μg i.t. on days 14 and 21) or tolerance (100 μg i.t. on days 0, 14 and 21).

    Techniques: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction

    Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized proteinase K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).

    Journal: Journal of Synchrotron Radiation

    Article Title: High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner

    doi: 10.1107/S1600577518010354

    Figure Lengend Snippet: Crystallography data collection and processing at FMX. Raster scans of ( a ) a bovine trypsin rod-shaped crystal and ( b ) 5–10 µm-sized proteinase K crystals loaded on MiTeGen loops. The raster-scan areas are shown as red-dashed rectangles; the corresponding heat maps acquired after the raster scans are shown in the insets. The grid coloring is the diffraction pattern spot count. ( c ) Clustering of the serial crystallography partial datasets based on unit-cell size. The data shown were acquired from raster scans of proteinase K microcrystals with a 200 Hz detector frame rate. Of these 279 partial datasets, 234 (blue dots) were indexed to c = 106.58 ± 0.41 Å, with 99.6% completeness (Table 1 ▸ ). The remaining 45 partial datasets (orange dots) with c = 110.06 ± 0.84 Å did not yield a complete dataset and were excluded from refinement. The unit-cell size was converted to Cartesian coordinates for better visualization. The data were clustered into two groups using a K -mean clustering algorithm. ( d ) Hierarchical cluster-analysis dendrogram for the proteinase K partial datasets acquired at a 750 Hz detector frame rate. The cutoff limit was set to 0.6 (dashed line).

    Article Snippet: Proteinase K crystals were grown on siliconized glass cover slips (hanging drop) by equilibrating 25 mg ml−1 proteinase K (Worthington Biochemical; LS004224) in 40 mM calcium chloride, 400 mM sodium nitrate and 50 mM BisTris (pH 6.5) over a reservoir containing 160 mM calcium chloride, 1.6 M sodium nitrate and 200 mM BisTris (pH 6.5).

    Techniques:

    (a) Number of pepsin, trypsin and chymotrypsin cleavage sites for 16-aa fragments of shrimp Tpm starting every six residues (i.e. 1–16, 7–22 …). Pink bars indicate fragments that overlap epitopes. (b) Epitopes (red) and a or d heptad position Ala-clusters (yellow) highlighted on a model of the Tpm coiled coil. (c) interhelical spacing is calculated between the d position on one chain and the a and a’ postions on the neighboring chain as ( da + da’ )/2. (d) mean interhelical spacing over the shrimp Tpm molecular dyanmics trajectory at acid and neutral pH. (e) .

    Journal: Structure (London, England : 1993)

    Article Title: Structural and dynamic properties of allergen and non-allergen forms of tropomyosin

    doi: 10.1016/j.str.2018.05.002

    Figure Lengend Snippet: (a) Number of pepsin, trypsin and chymotrypsin cleavage sites for 16-aa fragments of shrimp Tpm starting every six residues (i.e. 1–16, 7–22 …). Pink bars indicate fragments that overlap epitopes. (b) Epitopes (red) and a or d heptad position Ala-clusters (yellow) highlighted on a model of the Tpm coiled coil. (c) interhelical spacing is calculated between the d position on one chain and the a and a’ postions on the neighboring chain as ( da + da’ )/2. (d) mean interhelical spacing over the shrimp Tpm molecular dyanmics trajectory at acid and neutral pH. (e) .

    Article Snippet: Full length tropomyosin (shrimp or pig) was incubated for one hour at 37 °C in acidic buffer [0.2 M HCl−potassium chloride (KCl), pH 2], followed by the addition of pepsin A (Worthington Biochemical Corp., Lakewood, NJ) to give 0.33 Units of pepsin activity/μg tropomyosin substrate.

    Techniques:

    MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Journal: Nucleic Acids Research

    Article Title: 3?-Exonuclease resistance of DNA oligodeoxynucleotides containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine

    doi:

    Figure Lengend Snippet: MALDI-TOF mass spectra of SVPDE digests of modified DNA 16mers d(AACAGCCATATGXCCC): ( A ) X = O 6 -POB-dG, time-controlled digest; ( B ) X = O 6 -POB-dG, complete digest conditions; ( C ) O 6 -Me-dG-containing oligomers, controlled digest conditions. Arrows indicate the portion of the sequence represented in the spectra, and doubly charged ions are marked with #.

    Article Snippet: SVPDE and E.coli DNA polymerase I were purchased from Worthington Biochemicals (Lakewood, NJ).

    Techniques: Modification, Sequencing