tris cl 50  (Worthington Biochemical)


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  • 85
    Name:
    Neutral Protease Dispase Purified
    Description:
    Animal Origin Free Chromatographically purified A lyophilized powder
    Catalog Number:
    ls02100
    Price:
    70
    Size:
    10 mg
    Source:
    Bacillus polymyxa
    Cas Number:
    42613.33.2
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    Structured Review

    Worthington Biochemical tris cl 50
    Animal Origin Free Chromatographically purified A lyophilized powder
    https://www.bioz.com/result/tris cl 50/product/Worthington Biochemical
    Average 85 stars, based on 699 article reviews
    Price from $9.99 to $1999.99
    tris cl 50 - by Bioz Stars, 2020-08
    85/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Isolation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Mouse Assay:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Incubation:

    Article Title: CHARACTERIZING CALCIUM INFLUX VIA VOLTAGE- AND LIGAND-GATED CALCIUM CHANNELS IN EMBRYONIC ALLIGATOR NEURONS IN CULTURE
    Article Snippet: .. The brains were then transferred to a dissociation enzyme cocktail containing 1 µg/ml dispase (a mettalo, neutral protease; Worthington Biochemical Corporation, Lakewood, NJ) and 1.67 µg/ml collagenase (Worthington Biochemical Corporation) in ACSF and incubated for 10 min at 37°C. .. Then, the brain/dissociation enzyme cocktail was gently mechanically sheared by pipetting it through a 200 µl Eppendorf pipette at least 3 times and returned to 37°C for another 15 min.

    Article Title: Molecular architecture underlying fluid absorption by the developing inner ear
    Article Snippet: .. The tissues were pooled and incubated in 1 mg/ml collagenase (LS004194, Worthington, Lakewood, NJ) and 1 mg/ml dispase (LS02100, Worthington) in DMEM/F12 at 37°C for 10 min. .. The tissues were then transferred into 10% fetal bovine serum (FBS) (GIBCO) in DMEM/F12 where the epithelia were isolated from surrounding tissues.

    Article Title: Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment
    Article Snippet: .. For controlled proteolysis, hexon or AdV capsids were incubated with a 6-fold molar excess of dispase (neutral protease; Worthington Biochemicals, Freehold, NJ) over hexon in dispase buffer (100 mM Trizma-maleate, 120 mM NaCl, 2.5 mM CaCl2 ), and the pH was adjusted to either pH 7.4 (neutral) or pH 4.4 (acidic) for 5 min at 37°C until the reaction was stopped by the addition of either protein sample buffer (PSB) or 5 mM EDTA to the mixture. .. Sedimentation analysis of hexon was performed on linear 5 to 20% sucrose gradients of a volume of 1.4 ml in Tris-maleate buffer (50 mM Trizma-maleate, 10 mM NaCl, 1 mM EDTA, and 0.1% Tween 20) at the indicated pH values.

    FACS:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

    Purification:

    Article Title: Transcriptional Pathways in cPGI2-Induced Adipocyte Progenitor Activation for Browning
    Article Snippet: .. Adipose tissue digestion and SVF preparation Posterior subcutaneous adipose tissue (gluteal + inguinal) or the brown part of the interscapular fat was dissected, minced with scissors, and digested with 0.1 w.u./ml purified collagenase (LS005273, Worthington Biochemical, Lakewood, NJ, USA) and 2.4 U/ml Neutral Protease (LS02104, Worthington Biochemical) in Hank’s balanced salt solution (HBSS, Sigma-Aldrich, Munich, Germany) containing 4 mM calcium chloride and 0.05 mg/ml DNase I (1284932001, Roche Diagnostics, Grenzach-Wyhlen, Germany) for 50 min at 37°C in a shaker. ..

    Magnetic Cell Separation:

    Article Title: Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice
    Article Snippet: .. Microglia and brain leukocytes were isolated by MACS dissociation and fluorescence activated cell sorting (FACS) as previously described., Mice were transcardially perfused with PBS, and brains were processed using gentleMACS Dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) in an RPMI solution containing 0.2% Collagenase Type 3 (Worthington Biochemical Corporation, Lakewood, NJ) and Dispase (2 U/mL; Worthington Biochemical Corporation). .. DNase I (40 U/mL) was added after dissociation and incubated at 37°C for 10 min. Five percent fetal bovine serum in PBS/ethylenediaminetetraacetic acid solution was added to inactivate the digestion enzymes and passed through a 100-μm filter and centrifuged.

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