d ap5  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs d ap5
    NMDAR antagonists block TG-induced SOCE in rat cortical neurons but not HeLa cells. Average traces of intracellular Ca 2+ (F340/F380) levels obtained by ratiometric Fura-2AM analysis of neurons in the absence ( a ) or presence of 1 µM TTX ( c ), or in HeLa cells ( e ) treated with 50 µM <t>D-AP5</t> (green line) or 50 µM MM (red line) and untreated cells (blue line). Measurements were started in a medium with 0.5 mM EGTA, which was then replaced by a medium with 0.5 mM EGTA and either 2 µM TG + 50 µM D-AP5 or 2 µM TG + 50 µM MM. Finally, 2 mM CaCl 2 was added to the medium to trigger nSOCE with either 50 µM D-AP5 or 50 µM MM. F340/F380 values just before the addition of Ca 2+ were normalized to the same values (1). ( a – d ) The data represent n = 28 (Control), n = 12 (D-AP5), n = 20 (MM), n = 15 (Control + TTX), n = 19 (D-AP5 + TTX) and n = 18 (MM + TTX) independent experiments that were conducted on five different primary cultures, corresponding to 1160, 513, 780, 336, 390, and 710 analyzed cells that responded to KCl-induced membrane depolarization, respectively. ( e – f ) The data represents 17 independent measurements conducted in four different experiments corresponding to 1333 for control and 1315 for MM treated cells, respectively. ( b , d , f ) Summary data of panels ( a , c , e ) presented as the area under the curve (AUC) showing Ca 2+ influx, which was calculated from the moment immediately before adding Ca 2+ from minutes 7 to 11; ns (not significant), ** p
    D Ap5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d ap5/product/Alomone Labs
    Average 94 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    d ap5 - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons"

    Article Title: STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons

    Journal: Cells

    doi: 10.3390/cells9010160

    NMDAR antagonists block TG-induced SOCE in rat cortical neurons but not HeLa cells. Average traces of intracellular Ca 2+ (F340/F380) levels obtained by ratiometric Fura-2AM analysis of neurons in the absence ( a ) or presence of 1 µM TTX ( c ), or in HeLa cells ( e ) treated with 50 µM D-AP5 (green line) or 50 µM MM (red line) and untreated cells (blue line). Measurements were started in a medium with 0.5 mM EGTA, which was then replaced by a medium with 0.5 mM EGTA and either 2 µM TG + 50 µM D-AP5 or 2 µM TG + 50 µM MM. Finally, 2 mM CaCl 2 was added to the medium to trigger nSOCE with either 50 µM D-AP5 or 50 µM MM. F340/F380 values just before the addition of Ca 2+ were normalized to the same values (1). ( a – d ) The data represent n = 28 (Control), n = 12 (D-AP5), n = 20 (MM), n = 15 (Control + TTX), n = 19 (D-AP5 + TTX) and n = 18 (MM + TTX) independent experiments that were conducted on five different primary cultures, corresponding to 1160, 513, 780, 336, 390, and 710 analyzed cells that responded to KCl-induced membrane depolarization, respectively. ( e – f ) The data represents 17 independent measurements conducted in four different experiments corresponding to 1333 for control and 1315 for MM treated cells, respectively. ( b , d , f ) Summary data of panels ( a , c , e ) presented as the area under the curve (AUC) showing Ca 2+ influx, which was calculated from the moment immediately before adding Ca 2+ from minutes 7 to 11; ns (not significant), ** p
    Figure Legend Snippet: NMDAR antagonists block TG-induced SOCE in rat cortical neurons but not HeLa cells. Average traces of intracellular Ca 2+ (F340/F380) levels obtained by ratiometric Fura-2AM analysis of neurons in the absence ( a ) or presence of 1 µM TTX ( c ), or in HeLa cells ( e ) treated with 50 µM D-AP5 (green line) or 50 µM MM (red line) and untreated cells (blue line). Measurements were started in a medium with 0.5 mM EGTA, which was then replaced by a medium with 0.5 mM EGTA and either 2 µM TG + 50 µM D-AP5 or 2 µM TG + 50 µM MM. Finally, 2 mM CaCl 2 was added to the medium to trigger nSOCE with either 50 µM D-AP5 or 50 µM MM. F340/F380 values just before the addition of Ca 2+ were normalized to the same values (1). ( a – d ) The data represent n = 28 (Control), n = 12 (D-AP5), n = 20 (MM), n = 15 (Control + TTX), n = 19 (D-AP5 + TTX) and n = 18 (MM + TTX) independent experiments that were conducted on five different primary cultures, corresponding to 1160, 513, 780, 336, 390, and 710 analyzed cells that responded to KCl-induced membrane depolarization, respectively. ( e – f ) The data represents 17 independent measurements conducted in four different experiments corresponding to 1333 for control and 1315 for MM treated cells, respectively. ( b , d , f ) Summary data of panels ( a , c , e ) presented as the area under the curve (AUC) showing Ca 2+ influx, which was calculated from the moment immediately before adding Ca 2+ from minutes 7 to 11; ns (not significant), ** p

    Techniques Used: Blocking Assay

    SKF96365 increases NMDA-induced Ca 2+ levels. ( a ) Analysis of the [Ca 2+ ] i induced by NMDA (100 μM) and glycine (10 μM) in the presence of 5 μM nimodipine, 30 μM CNQX, and 50 μM MM; 50 μM D-AP5 or 30 μM SKF96365 (SKF) based on ratiometric measurements with Fura2-AM. F 340 /F 380 values just before adding the NMDAR agonist were normalized to the same values (1). The data represent n independent experiments that were conducted on four different primary cultures corresponding to 516 (NMDA, n = 13), 305 (NMDA-MM, n = 10), 245 (NMDA-DAP, n = 10) and 624 (NMDA-SKF, n = 19) analyzed cells. ( b ) Summary of data from ( a ) shown as area under the curve (AUC), which was calculated from the moment immediately before the addition of NMDA. ** p
    Figure Legend Snippet: SKF96365 increases NMDA-induced Ca 2+ levels. ( a ) Analysis of the [Ca 2+ ] i induced by NMDA (100 μM) and glycine (10 μM) in the presence of 5 μM nimodipine, 30 μM CNQX, and 50 μM MM; 50 μM D-AP5 or 30 μM SKF96365 (SKF) based on ratiometric measurements with Fura2-AM. F 340 /F 380 values just before adding the NMDAR agonist were normalized to the same values (1). The data represent n independent experiments that were conducted on four different primary cultures corresponding to 516 (NMDA, n = 13), 305 (NMDA-MM, n = 10), 245 (NMDA-DAP, n = 10) and 624 (NMDA-SKF, n = 19) analyzed cells. ( b ) Summary of data from ( a ) shown as area under the curve (AUC), which was calculated from the moment immediately before the addition of NMDA. ** p

    Techniques Used:

    2) Product Images from "STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons"

    Article Title: STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons

    Journal: Cells

    doi: 10.3390/cells9010160

    NMDAR antagonists block TG-induced SOCE in rat cortical neurons but not HeLa cells. Average traces of intracellular Ca 2+ (F340/F380) levels obtained by ratiometric Fura-2AM analysis of neurons in the absence ( a ) or presence of 1 µM TTX ( c ), or in HeLa cells ( e ) treated with 50 µM D-AP5 (green line) or 50 µM MM (red line) and untreated cells (blue line). Measurements were started in a medium with 0.5 mM EGTA, which was then replaced by a medium with 0.5 mM EGTA and either 2 µM TG + 50 µM D-AP5 or 2 µM TG + 50 µM MM. Finally, 2 mM CaCl 2 was added to the medium to trigger nSOCE with either 50 µM D-AP5 or 50 µM MM. F340/F380 values just before the addition of Ca 2+ were normalized to the same values (1). ( a – d ) The data represent n = 28 (Control), n = 12 (D-AP5), n = 20 (MM), n = 15 (Control + TTX), n = 19 (D-AP5 + TTX) and n = 18 (MM + TTX) independent experiments that were conducted on five different primary cultures, corresponding to 1160, 513, 780, 336, 390, and 710 analyzed cells that responded to KCl-induced membrane depolarization, respectively. ( e – f ) The data represents 17 independent measurements conducted in four different experiments corresponding to 1333 for control and 1315 for MM treated cells, respectively. ( b , d , f ) Summary data of panels ( a , c , e ) presented as the area under the curve (AUC) showing Ca 2+ influx, which was calculated from the moment immediately before adding Ca 2+ from minutes 7 to 11; ns (not significant), ** p
    Figure Legend Snippet: NMDAR antagonists block TG-induced SOCE in rat cortical neurons but not HeLa cells. Average traces of intracellular Ca 2+ (F340/F380) levels obtained by ratiometric Fura-2AM analysis of neurons in the absence ( a ) or presence of 1 µM TTX ( c ), or in HeLa cells ( e ) treated with 50 µM D-AP5 (green line) or 50 µM MM (red line) and untreated cells (blue line). Measurements were started in a medium with 0.5 mM EGTA, which was then replaced by a medium with 0.5 mM EGTA and either 2 µM TG + 50 µM D-AP5 or 2 µM TG + 50 µM MM. Finally, 2 mM CaCl 2 was added to the medium to trigger nSOCE with either 50 µM D-AP5 or 50 µM MM. F340/F380 values just before the addition of Ca 2+ were normalized to the same values (1). ( a – d ) The data represent n = 28 (Control), n = 12 (D-AP5), n = 20 (MM), n = 15 (Control + TTX), n = 19 (D-AP5 + TTX) and n = 18 (MM + TTX) independent experiments that were conducted on five different primary cultures, corresponding to 1160, 513, 780, 336, 390, and 710 analyzed cells that responded to KCl-induced membrane depolarization, respectively. ( e – f ) The data represents 17 independent measurements conducted in four different experiments corresponding to 1333 for control and 1315 for MM treated cells, respectively. ( b , d , f ) Summary data of panels ( a , c , e ) presented as the area under the curve (AUC) showing Ca 2+ influx, which was calculated from the moment immediately before adding Ca 2+ from minutes 7 to 11; ns (not significant), ** p

    Techniques Used: Blocking Assay

    SKF96365 increases NMDA-induced Ca 2+ levels. ( a ) Analysis of the [Ca 2+ ] i induced by NMDA (100 μM) and glycine (10 μM) in the presence of 5 μM nimodipine, 30 μM CNQX, and 50 μM MM; 50 μM D-AP5 or 30 μM SKF96365 (SKF) based on ratiometric measurements with Fura2-AM. F 340 /F 380 values just before adding the NMDAR agonist were normalized to the same values (1). The data represent n independent experiments that were conducted on four different primary cultures corresponding to 516 (NMDA, n = 13), 305 (NMDA-MM, n = 10), 245 (NMDA-DAP, n = 10) and 624 (NMDA-SKF, n = 19) analyzed cells. ( b ) Summary of data from ( a ) shown as area under the curve (AUC), which was calculated from the moment immediately before the addition of NMDA. ** p
    Figure Legend Snippet: SKF96365 increases NMDA-induced Ca 2+ levels. ( a ) Analysis of the [Ca 2+ ] i induced by NMDA (100 μM) and glycine (10 μM) in the presence of 5 μM nimodipine, 30 μM CNQX, and 50 μM MM; 50 μM D-AP5 or 30 μM SKF96365 (SKF) based on ratiometric measurements with Fura2-AM. F 340 /F 380 values just before adding the NMDAR agonist were normalized to the same values (1). The data represent n independent experiments that were conducted on four different primary cultures corresponding to 516 (NMDA, n = 13), 305 (NMDA-MM, n = 10), 245 (NMDA-DAP, n = 10) and 624 (NMDA-SKF, n = 19) analyzed cells. ( b ) Summary of data from ( a ) shown as area under the curve (AUC), which was calculated from the moment immediately before the addition of NMDA. ** p

    Techniques Used:

    3) Product Images from "Expression of TRPV1 channels by Cajal‐Retzius cells and layer‐specific modulation of synaptic transmission by capsaicin in the mouse hippocampus"

    Article Title: Expression of TRPV1 channels by Cajal‐Retzius cells and layer‐specific modulation of synaptic transmission by capsaicin in the mouse hippocampus

    Journal: The Journal of Physiology

    doi: 10.1113/JP275685

    Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).
    Figure Legend Snippet: Responses to capsaicin are not dependent on the integrity of synaptic transmission and/or action potential generation A , left panel, control image in the presence of antagonists of synaptic ionotropic receptors (syn blockers; NBQX, 20 μM; D‐AP5, 50 μM; picrotoxinin, 50 μM) and after the addition of capsaicin (1 μM). Middle panel, similar to left panel with the additional constant presence of TTX. Right panel, control image in the presence of TTX and cadmium (150 μM) and after the introduction of capsaicin (1 μM). Notice that none of the experimental conditions was successful in preventing responses to capsaicin. Colour scale bar in arbitrary units. B , F / F 0 traces obtained from several CRs shown in different colours and superimposed in the same experimental conditions of the panels in A . C , summary plots from several experiments quantifying the peak of the F / F 0 response (left), its half‐width (middle) and latency (right).

    Techniques Used: Transmission Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs d ap5
    D Ap5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d ap5/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d ap5 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results