Ap5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 94 stars, based on 1 article reviews
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1) Product Images from "Translocation of CaMKII to dendritic microtubules supports the plasticity of local synapses"
Article Title: Translocation of CaMKII to dendritic microtubules supports the plasticity of local synapses
Journal: The Journal of Cell Biology
Figure Legend Snippet: Activity-dependent translocation of αCaMKII to dendritic sites near synapses. (A) Hippocampal neuron (12 DIV) expressing mGFP-αCaMKII and mCherry imaged before, during, and after stimulation with 0Mg 2+ /Gly for 5 min. (B, i) Pseudocolor image from dendrite in A. Kymograph (ii–iii) and time-lapse (iv) analyses of the change in the fluorescent intensity ratio (mGFP-αCaMKII/mCherry) over time across the dendrite (i–ii, black line) and across the spine (iii, yellow box, red line). Stimulation period is indicated (Stim). (iv) n = 4 spines and subdendritic regions from the neuron shown in A. Red and white arrows (or brackets) point, respectively, to synaptic and dendritic sites where CaMKII translocated. Bars: (neuron) 10 µm; (dendrite) 5 µm. (C) Normalized ratio (±SEM) of dendritic segment where mGFP-αCaMKII accumulated over total dendritic length after a 5-min 0Mg 2+ /Gly stimulation (Vehicle), in the presence of 50 µM AP5, 1 μM TTX, 10 μM Cd 2+ , or 10 μM CPA. n = 7–24 neurons per condition. *, P
Techniques Used: Activity Assay, Translocation Assay, Expressing
2) Product Images from "HIV and opiates dysregulate K+-Cl− cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice"
Article Title: HIV and opiates dysregulate K+-Cl− cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice
Journal: Neurobiology of disease
Figure Legend Snippet: hNeurons lose KCC2 immunoreactivity after exposure to HIV-Tat ± morphine. ( A ) Representative images of hNeuron cultures immunolabeled for MAP2 and KCC2. Loss of KCC2 immunoreactivity can be noted in cells treated with both 50 nM Tat 1-86 and 50 nM Tat 1-86 + morphine. Rescue is seen in 50 nM Tat 1-86 + AP5 (scale bar = 50 μm). ( B, C ) The percentage of cells immunoreactive for KCC2. Both 6 h ( B ) and 24 h ( C ) exposure to 50 - 100 nM Tat 1-86 ± morphine results in significant decrease in KCC2 immunoreactivity compared to their respective controls ( † p
Techniques Used: Immunolabeling