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cd45 pe catalog number 103 106  (Revvity Signals)
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Revvity Signals cd45 pe catalog number 103 106
Cd45 Pe Catalog Number 103 106, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bei catalog number nr 52516  (BEI Resources)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
Bei Catalog Number Nr 52516, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbudce4 1 catalog number v53220 all  (Thermo Fisher)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
Pbudce4 1 Catalog Number V53220 All, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
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pcdna3 1 catalog number v79020  (Thermo Fisher)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
Pcdna3 1 Catalog Number V79020, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 hygro catalog number v87020  (Thermo Fisher)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
Pcdna3 1 Hygro Catalog Number V87020, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membrane bound coumarin phospholipid fret donor thermofisher scientific catalog number k1017 cas number 393782 57 5 tetradecanoic acid  (Thermo Fisher)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
Membrane Bound Coumarin Phospholipid Fret Donor Thermofisher Scientific Catalog Number K1017 Cas Number 393782 57 5 Tetradecanoic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna4 myc his catalog number v86320  (Thermo Fisher)
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from <t>BEI</t> Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .
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(A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from BEI Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .

Journal: bioRxiv

Article Title: RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera

doi: 10.1101/2025.03.11.642476

Figure Lengend Snippet: (A) Correlation between neutralization titers against RSV A2 measured with our pseudovirus neutralization assay (y-axis) versus previously published values measured using full-length replicative RSV A2 (x-axis). 1:1 is shown by the gray dashed line. Neutralization titers are the reciprocal sera dilution at 50% fraction infectivity. Each point represents the geometric mean neutralization titer of two independent experiments for a different human serum sample. (B) Neutralization measurements are highly reproducible. Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strains against three different reference sera from BEI Resources. Points indicate the mean ± standard error of technical repeats of experiments performed on the same day. Different replicates indicate independent experiments performed on different days. See for additional independent replicates validating the reproducibility. (C) Neutralization titers for the three reference sera from BEI Resources match published values. Plots show neutralization titers for pseudoviruses expressing F and G from RSV A2 or B1 strains versus published neutralization values , . Neutralization titers were standardized to International Units per milliliter (IU/mL) as described in .

Article Snippet: A lentiviral backbone plasmid that uses a CMV promoter to express luciferase followed by an IRES and ZsGreen is available from BEI Resources (BEI catalog number NR-52516) .

Techniques: Neutralization, Infection, Expressing

(A) Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strain by the BEI High reference serum NR4021 and WHO International Reference Antiserum to RSV (NIBSC 16/284) for six independent experimental replicates performed on three separate days by two different people ( shows just two of the replicates for BEI High reference serum NR4021). Points for each replicate indicate the mean ± standard error of two technical repeats of that experiment. (B) Table detailing neutralization titers for the indicated sera and pseudoviruses. The number of replicates indicates the number of independent experiments for which neutralization titers were measured. The geometric mean NT50 represents the reciprocal sera dilution at 50% fraction infectivity. The measured geometric mean titers for the WHO International Reference Antiserum to RSV (NIBSC 16/284) against RSV A2 and B1 were used to calculate a conversion factor based on the standardized potency of these sera (2000/geometric mean titer). This factor converts measurements from our assay into International Units per milliliter (IU/mL) , . Namely, to convert titers from our RSV pseudovirus assay into IU/ml, those titers should be multiplied by 0.196 for A2, and 0.183 for B1. Neutralization titers for the BEI Resources reference sera were converted to IU/mL by multiplying the measured geometric mean titer by the corresponding conversion factor. Those titers in IU/ml are then plotted versus the known values for the reference sera in .

Journal: bioRxiv

Article Title: RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera

doi: 10.1101/2025.03.11.642476

Figure Lengend Snippet: (A) Neutralization curves of pseudoviruses with F and G from the RSV A2 or B1 strain by the BEI High reference serum NR4021 and WHO International Reference Antiserum to RSV (NIBSC 16/284) for six independent experimental replicates performed on three separate days by two different people ( shows just two of the replicates for BEI High reference serum NR4021). Points for each replicate indicate the mean ± standard error of two technical repeats of that experiment. (B) Table detailing neutralization titers for the indicated sera and pseudoviruses. The number of replicates indicates the number of independent experiments for which neutralization titers were measured. The geometric mean NT50 represents the reciprocal sera dilution at 50% fraction infectivity. The measured geometric mean titers for the WHO International Reference Antiserum to RSV (NIBSC 16/284) against RSV A2 and B1 were used to calculate a conversion factor based on the standardized potency of these sera (2000/geometric mean titer). This factor converts measurements from our assay into International Units per milliliter (IU/mL) , . Namely, to convert titers from our RSV pseudovirus assay into IU/ml, those titers should be multiplied by 0.196 for A2, and 0.183 for B1. Neutralization titers for the BEI Resources reference sera were converted to IU/mL by multiplying the measured geometric mean titer by the corresponding conversion factor. Those titers in IU/ml are then plotted versus the known values for the reference sera in .

Article Snippet: A lentiviral backbone plasmid that uses a CMV promoter to express luciferase followed by an IRES and ZsGreen is available from BEI Resources (BEI catalog number NR-52516) .

Techniques: Neutralization, Infection