ccrf cem cells (ATCC)
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ATCC
ccrf cem cells
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccrf cem cells - by Bioz Stars,
2024-09
96/100 stars
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ccrf cem atcc crm ccl 119 (ATCC)
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ATCC
ccrf cem atcc crm ccl 119
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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2024-09
86/100 stars
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ccrf cem atcc crm ccl 119 (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
86
Structured Review
ATCC
ccrf cem atcc crm ccl 119
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem atcc crm ccl 119/product/ATCC
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem atcc crm ccl 119/product/ATCC
Average 86 stars, based on 1 article reviews
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ccrf cem atcc crm ccl 119 - by Bioz Stars,
2024-09
86/100 stars
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ccrf cem atcc crm ccl 119 (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
86
Structured Review
ATCC
ccrf cem atcc crm ccl 119
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem atcc crm ccl 119/product/ATCC
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Atcc Crm Ccl 119, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem atcc crm ccl 119/product/ATCC
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ccrf cem atcc crm ccl 119 - by Bioz Stars,
2024-09
86/100 stars
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ccrf cem cells (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
96
Structured Review
ATCC
ccrf cem cells
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ccrf cem cells - by Bioz Stars,
2024-09
96/100 stars
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ccrf cem cells (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
95
Structured Review
ATCC
ccrf cem cells
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
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ccrf cem cells - by Bioz Stars,
2024-09
95/100 stars
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ccrf cem (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
96
Structured Review
ATCC
ccrf cem
Ccrf Cem, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem/product/ATCC
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem/product/ATCC
Average 96 stars, based on 1 article reviews
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ccrf cem - by Bioz Stars,
2024-09
96/100 stars
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ccrf cem cells (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
95
Structured Review
ATCC
ccrf cem cells
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ccrf cem cells - by Bioz Stars,
2024-09
95/100 stars
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1) Product Images from "Receptor-targeted aptamer-siRNA conjugate-directed transcriptional regulation of HIV-1"
Article Title: Receptor-targeted aptamer-siRNA conjugate-directed transcriptional regulation of HIV-1
Journal: Theranostics
doi: 10.7150/thno.23085
Figure Legend Snippet: Aptamer-directed TGS. ( A ) A schematic is shown depicting gp120 aptamer conjugates containing the LTR-362 siRNA directed to transcriptionally silence HIV-1 LTR activity and inhibit HIV-1 expression. ( B ) The candidate gp120-aptamer siRNA chimeras and controls assessed are shown. ( C ) The effects of the various gp120-aptamer-siRNA chimeras and controls on HIV-1 expression in HIV-1-infected CCRF-CEM cells following one 800 nM treatment. A schematic depicting the experimental procedure is shown along with p24 values from days 3-10 post-aptamer treatment.
Techniques Used: Activity Assay, Expressing, Infection
Figure Legend Snippet: Gp120 aptamer-siRNA 362 represses HIV-1 by transcriptional gene silencing. ( A ) A schematic depicting the experimental plan to test the various aptamer conjugates. ( B ) The effects of the various aptamer siRNA conjugates on HIV-1 expression in HIV-1-IIIB-infected CCRF-CEM cells (MOI=0.001). ( C-E ) Nuclear run-on analysis of LTR transcription in differentially treated aptamer-siRNA conjugates in ( C ) the absence of any drug and ( D ) the presence of TSA (1 µM) or ( E ) 5' AzaC (4 µM). ( F-H ) expression of HIV p24 in ( F ) untreated, TSA- or 5' AzaC-treated HIV-1-infected CCRF-CEM cultures. ( G ) Nuclear localization of the antisense strand of LTR-362-directed siRNAs following gp120 aptamer delivery in gp120 positive CHO-WT cells. ( H ) The cytoplasmic vs. nuclear distribution of siRNA Tat/rev , LTR-362 siRNA, and Scr siRNA in aptamer conjugate-treated HIV-1-infected CCRF-CEM cells. For B-F triplicate treated cells are shown with the standard deviations and (*) representing p <0.05 from a paired T-test.
Techniques Used: Expressing, Infection
chromatin isolation (ATCC)
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ATCC manufactures this product
96
Structured Review
ATCC
chromatin isolation
Chromatin Isolation, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromatin isolation/product/ATCC
Average 96 stars, based on 1 article reviews
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Chromatin Isolation, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromatin isolation/product/ATCC
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
chromatin isolation - by Bioz Stars,
2024-09
96/100 stars
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ccrf cem cells (ATCC)
ATCC is a verified supplier
ATCC manufactures this product
95
Structured Review
ATCC
ccrf cem cells
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Ccrf Cem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccrf cem cells/product/ATCC
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ccrf cem cells - by Bioz Stars,
2024-09
95/100 stars
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1) Product Images from "Microtubules Regulate Migratory Polarity through Rho/ROCK Signaling in T Cells"
Article Title: Microtubules Regulate Migratory Polarity through Rho/ROCK Signaling in T Cells
Journal: PLoS ONE
doi: 10.1371/journal.pone.0008774
Figure Legend Snippet: CCRF-CEM T cells were pre-treated with or without 10 µM taxol for 30 min or 20 µM nocodazole for 10 min, plated on ICAM-1-coated dishes, and stimulated with 1 nM CXCL12. Cell migration was monitored by time-lapse microscopy. (A) Frames from movies of control, taxol or nocodazole-treated CCRF-CEM cells at 0, 4 and 8 min are shown. Outlines of the cells at 0 (gray), 4 (yellow) and 8 (red) min reveal the migrating paths. Bars = 10 µm. (B) Migration tracks of cells (10 to 16 cells for each condition) are displayed as direction plots. The mean migration speed of cells ± SEM is shown. Data shown are representative of 4 independent experiments. (C) Frames of a nocodazole-treated CCRF-CEM cell . White arrowheads indicate bleb-like protrusions. (D) Frames of a control (top panels; ) and nocodazole-treated (bottom panels; ) CCRF-CEM cell are shown. Right panels show kymographs of the region marked by the white bar in the left panels. White arrows (bottom panel) indicate extension of progressive blebs. Bar = 10 µm.
Techniques Used: Migration, Time-lapse Microscopy
Figure Legend Snippet: CCRF-CEM cells were pre-treated with or without taxol or nocodazole, plated on ICAM-1, and then stimulated with 20 nM CXCL12 for 5 min. Cells were fixed and stained with anti-α-tubulin antibody (green) and TRITC-conjugated phalloidin to show actin filaments (red). Representative confocal images are shown. Bar = 10 µm. The white arrows in nocodazole-treated cells indicate bleb-like membrane protrusions.
Techniques Used: Staining
Figure Legend Snippet: (A) Quantification of T cell polarization. T cell morphology was classified in 3 categories: 1) non-polarized (white), where the cells had a spherical morphology, 2) elongated (gray), where the cells had an elongated cell shape but no diametric polarization of F-actin and α-tubulin, and 3) migratory polarized (black), where the cells were elongated and had diametric distribution of F-actin at the leading edge and the MTOC (identified with anti-α-tubulin antibody) behind the nucleus; n = 110 to 150 cells for each condition from 3 independent experiments. (B) Nocodazole alters F-actin levels in T cells. Flow cytometric analysis of the F-actin content of CCRF-CEM cells incubated with 10 µM Y-27632 for 30 min and/or 20 µM nocodazole for 10 min. Data are shown as a percentage of the mean fluorescence of untreated cells. Data are the mean of three independent experiments +/− SEM. *p<0.05 compared to control cells.
Techniques Used: Incubation, Fluorescence
Figure Legend Snippet: CCRF-CEM cells were pre-treated with or without 10 µM taxol for 30 min or 20 µM nocodazole for 10 min, plated on ICAM-1, and then stimulated with 20 nM CXCL12 for 5 min before fixation. (A) Cells were stained with anti-ICAM-3 antibody (green) and phalloidin to show actin filaments (red). Small uropod-like protrusions where ICAM-3 accumulates in nocodazole-treated cells are indicated with white asterisks. Representative confocal images are shown. (B) Cells were stained with anti-RhoA antibody (green) and anti-phospho-ERM antibody (red). Bleb-like membrane protrusions are indicated with white arrows and small uropod-like protrusions where brighter phospho-ERM staining is observed are shown with white asterisks in nocodazole-treated cells. Bar = 10 µm.
Techniques Used: Staining
Figure Legend Snippet: (A) Cells were pretreated with or without 10 µM taxol for 30 min and subsequently with or without 20 µM nocodazole (noco) for 10 min, plated on ICAM-1 for 5 min, then lysed to determine the levels of active GTP-bound RhoA by a GST-Rhotekin-RBD pulldown assay. The graph represents a quantification of densitometry results, normalised to total RhoA and indicated as fold increase in RhoA-GTP over the level in control cells. Data shown are representative of 3 independent experiments. (B) CCRF-CEM cells were pre-treated with (black) or without (white) 20 µM nocodazole (noco) for 10 min, plated on ICAM-1, then stimulated with 50 nM CXCL12 for the indicated time periods. Levels of active GTP-bound RhoA were determined by a GST-Rhotekin-RBD pulldown assay. The graph represents a quantification of densitometry results, normalised to total RhoA and indicated as fold increase in RhoA-GTP over the level in resting control cells (0 min). Data shown are representative of 4 independent experiments. (C) Western blot of phospho-cofilin and total cofilin. CCRF-CEM cells were treated with 10 µM Y-27632 (Y) for 30 min or 20 µM nocodazole (noco) for 10 min, plated on ICAM-1 then stimulated with 50 nM CXCL12 for 5 min, lysed and analysed by western blotting using anti-phospho-cofilin (Ser3) antibody and cofilin antibody. Data represent quantification of densitometry results from 3 independent experiments (Mean ± SD), normalised to total cofilin and indicated as fold increase in phospho-cofilin over the level in untreated control cells.
Techniques Used: Western Blot
Figure Legend Snippet: CCRF-CEM T cells were treated with or without 10 µM Y-27632 for 30 min and subsequently with or without 20 µM nocodazole (noco) for 10 min, then stimulated with 1 nM CXCL12 on ICAM-1-coated dishes. (A) Cell migration was monitored by time-lapse microscopy. Examples of cell trajectories of 10 to 15 cells are shown and mean migration speed ± SEM (n = 10–15). (B) Migration parameters were calculated from cell tracks. The kinetic data of 30 to 40 cells in each condition from three independent experiments are shown. *p<0.05, compared to control cells, or # p<0.05 compared to nocodazole-treated cells, indicated with bridges, Student's t-test. (C) Chemotaxis of CCRF-CEM cells towards CXCL12. Cells were pre-incubated with 10 µM Y-27632 for 30 min and/or 20 µM nocodazole for the 10 min before adding to ICAM-coated transwells. Migrated cells were counted after 60 min. **p<0.01, compared to control cells, or # p<0.05 compared to nocodazole-treated cells, indicated with bridge, ANOVA. (D) CCRF-CEM cell migration was monitored by time-lapse microscopy. Y-27632 (10 µM) was added to nocodazole-treated cells (20 µM) (noco) at 12 min ( ; bottom panels). A control migrating cell is marked with an asterisk (top panels). White arrow indicates a tail induced following Y-27632 addition; white arrowhead indicates restored lamellipodium. Bar = 10 µm.
Techniques Used: Migration, Time-lapse Microscopy, Chemotaxis Assay, Incubation
Figure Legend Snippet: (A) CCRF-CEM cells were treated with or without 10 µM Y-27632 (Y) and 20 µM nocodazole (noco) then plated on ICAM-1 and stimulated with 20 nM CXCL12 for 5 min. Cells were fixed and stained with anti-RhoA antibody (green) and anti-phospho-ERM antibody (red). Representative confocal projection images reconstructed from z-stacks of 15 to 25 frames with 0.4 µm interval are shown. Note that Y-27632 prevents nocodazole-induced membrane blebbing (indicated with white arrows) and restricts phospho-ERMs to the uropod. Bar = 10 µm. (B) Localization of F-actin (red) and ICAM-3 (green) in CXCL12-stimulated CCRF-CEM cells on ICAM-1. Cells were pre-treated with 20 µM nocodazole (noco) and 10 µM Y-27632 (Y) as indicated. Representative confocal images and line-plot graphs of subcellular distributions of F-actin and ICAM-3 in each condition. Line-plot graphs indicate fluorescent intensity (FI) of phalloidin (F-actin) (red) and ICAM-3 (green) along the white arrows indicated in merge images. (C) Percentage of CCRF-CEM cells with diametric polarization of F-actin and ICAM-3. Data are from n = 220 to 250 cells in each condition which are collected from 3 independent experiments and represent mean ± SD. *p<0.03, compared to control cells, Student's t-test. (D) CCRF-CEM cells were treated with or without 10 µM Y-27632 (Y) for 30 min and subsequently with or without 20 µM nocodazole (noco) for 10 min, incubated with latex-beads coated with ICAM-1 (5 µg/ml) and CXCL12 (20 nM), and then fixed and stained with phalloidin to show actin filaments (red) and anti-ICAM-3 antibody (green). Representative images are shown. Asterisks indicate the bead-cell contact sites.
Techniques Used: Staining, Incubation
Figure Legend Snippet: (A) HUVECs were left untreated (control) or were incubated with 0.1 µM nocodazole for 60 min (noco) and/or 5 µM Y-27632 (Y) for 15 min then 0.1 µM nocodazole for 60 min (Y+noco). To visualize MT distribution, cells were extracted with 0.05% Triton X-100 to remove monomeric tubulin, then stained with anti-α-tubulin antibodies. The areas marked with a white rectangle have been enlarged and pseudocoloured using the image analysis program LaserPix to help visualise MT morphology in untreated and treated cells. (B, C) CCRF-CEM cells were treated with or without 10 µM Y-27632 (Y), 0.4 µM H-1152 (H) or 50 µM blebbistatin (bleb) for 30 min, then with or without 20 µM nocodazole (noco) for 10 min, then plated on ICAM-1 and stimulated with 20 nM CXCL12 for 5 min. Localization of acetylated tubulin (Ace-TUB) was examined by staining with anti-Ace-TUB antibody. (B) Representative confocal images; the outline of cells is indicated by white lines; (C) percentage of filamentous Ace-TUB positive cells (top), and % of morphologically polarized cells (with uropod and a leading edge) containing filamentous Ace-TUB (bottom). Data represent mean ± SEM from 3 independent experiments, n = 150 to 250 cells in each condition, ## p<0.01 for % of Ace-TUB positive cells compared to nocodazole-treated cells in the top panel, **p<0.01 for % of morphologically polarized cells compared to untreated control, or # p<0.05 compared to nocodazole-treated cells, to Y-27632+nocodazole-treated cells or to H-1152+nocodazole-treated cells (indicated with bridge), Student's t-test. (D) Western blot analysis of Ace-TUB and Glu-TUB levels in CCRF-CEM T cell lysates treated with or without 10 µM Y-27632 (Y) for 30 min and subsequently with or without 20 µM nocodazole (noco) for 10 min. Data represent quantification of densitometry results (Mean ± SD) of three independent experiments, normalised to the total α-TUB level and indicated as fold increase in Ace-TUB or Glu-TUB over the level in control cells; *p<0.05, **p<0.01 for Ace-TUB, or # p<0.05, # p<0.01 for Glu-TUB compared to control (without bridge), or to nocodazole-treated cells (indicated with bridge); Student's t-test.
Techniques Used: Incubation, Staining, Western Blot
human t acute lymphoblastic leukemia (ATCC)
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ATCC manufactures this product
96
Structured Review
ATCC
human t acute lymphoblastic leukemia
Human T Acute Lymphoblastic Leukemia, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Human T Acute Lymphoblastic Leukemia, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human t acute lymphoblastic leukemia/product/ATCC
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human t acute lymphoblastic leukemia - by Bioz Stars,
2024-09
96/100 stars