Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Article Title: Studies on the Synthesis, Photophysical and Biological Evaluation of Some Unsymmetrical Meso-Tetrasubstituted Phenyl Porphyrins
Figure Lengend Snippet: Cellular uptake of the asymmetric P2.2 and symmetric P2.1 compounds (10 µM) by human monocytic SC cells in 24 h culture. Cellular uptake was evaluated by flow cytometry (498 nm excitation and emmission in the red FL3 channel). Results are presented as: ( a ) mean fluorescence (geomean, arbitrary units) and the coefficient of variation (CV) of cellular fluorescence distribution; ( b ) representative histogram ( Control, P2.1 , P2.2 ).
Article Snippet: In some experiments we used the human monocytic SC cell line (ATCC® CRL-9855™) that was grown in suspension in IMDM culture medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Biochrom), antibiotic-antimycotic solution (Sigma-Aldrich), 1% HT supplement (Thermo Fisher Scientific, Waltham, MA, USA) and 0.1% 2-mercaptoethanol (Sigma).
Techniques: Flow Cytometry, Cytometry, Fluorescence