8e5 cell line 8e5a atcc crl 8993tm  (ATCC)


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    ATCC 8e5 cell line 8e5a atcc crl 8993tm
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Cell Line 8e5a Atcc Crl 8993tm, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cell line 8e5a atcc crl 8993tm/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8e5 cell line 8e5a atcc crl 8993tm - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy"

    Article Title: Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-13581-8

    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    Figure Legend Snippet: Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

    Techniques Used:

    8e5 cell line 8e5a atcc crl 8993tm  (ATCC)


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    ATCC 8e5 cell line 8e5a atcc crl 8993tm
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Cell Line 8e5a Atcc Crl 8993tm, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cell line 8e5a atcc crl 8993tm/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8e5 cell line 8e5a atcc crl 8993tm - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy"

    Article Title: Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-13581-8

    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    Figure Legend Snippet: Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

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    8e5 crl 8993 cells  (ATCC)


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    ATCC 8e5 crl 8993 cells
    8e5 Crl 8993 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 crl 8993 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
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    8e5 crl 8993 cells - by Bioz Stars, 2023-01
    94/100 stars

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    8e5 cell line  (ATCC)


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    ATCC 8e5 cell line
    8e5 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cell line/product/ATCC
    Average 94 stars, based on 1 article reviews
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    8e5 cell line - by Bioz Stars, 2023-01
    94/100 stars

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    8e5 lav cell line  (ATCC)


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    ATCC 8e5 lav cell line
    8e5 Lav Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 lav cell line/product/ATCC
    Average 94 stars, based on 1 article reviews
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    8e5 lav cell line - by Bioz Stars, 2023-01
    94/100 stars

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    hiv env  (ATCC)


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    ATCC hiv env
    Hiv Env, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hiv env - by Bioz Stars, 2023-01
    94/100 stars

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    hiv env  (ATCC)


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    ATCC hiv env
    Hiv Env, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv env/product/ATCC
    Average 94 stars, based on 1 article reviews
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    hiv env - by Bioz Stars, 2023-01
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    8e5 cells  (ATCC)


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    ATCC 8e5 cells
    8e5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
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    8e5 cells - by Bioz Stars, 2023-01
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    crl 8993  (ATCC)


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    ATCC crl 8993
    Crl 8993, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    8e5 cells  (ATCC)


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    ATCC 8e5 cells
    Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells <t>(8E5)</t> by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.
    8e5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    8e5 cells - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "One-domain CD4 Fused to Human Anti-CD16 Antibody Domain Mediates Effective Killing of HIV-1-Infected Cells"

    Article Title: One-domain CD4 Fused to Human Anti-CD16 Antibody Domain Mediates Effective Killing of HIV-1-Infected Cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-07966-3

    Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells (8E5) by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.
    Figure Legend Snippet: Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells (8E5) by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.

    Techniques Used: Incubation, Staining, Infection, Activity Assay, Negative Control

    8e5 cells  (ATCC)


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    ATCC 8e5 cells
    Comparison between dPCR and qPCR results from 18 PBMC samples from HIV-positive patients, expressed as HIV DNA copies per million cells. The three samples in which HIV DNA was not detected by dPCR are not plotted. ( a ) qPCR quantities calculated assuming one HIV DNA copy per <t>8E5</t> cell. ( b ) qPCR quantities calculated assuming 0.6 HIV DNA copy per 8E5 cell as determined experimentally by dPCR NB. The dashed line represents equivalence.
    8e5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR"

    Article Title: Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01221-5

    Comparison between dPCR and qPCR results from 18 PBMC samples from HIV-positive patients, expressed as HIV DNA copies per million cells. The three samples in which HIV DNA was not detected by dPCR are not plotted. ( a ) qPCR quantities calculated assuming one HIV DNA copy per 8E5 cell. ( b ) qPCR quantities calculated assuming 0.6 HIV DNA copy per 8E5 cell as determined experimentally by dPCR NB. The dashed line represents equivalence.
    Figure Legend Snippet: Comparison between dPCR and qPCR results from 18 PBMC samples from HIV-positive patients, expressed as HIV DNA copies per million cells. The three samples in which HIV DNA was not detected by dPCR are not plotted. ( a ) qPCR quantities calculated assuming one HIV DNA copy per 8E5 cell. ( b ) qPCR quantities calculated assuming 0.6 HIV DNA copy per 8E5 cell as determined experimentally by dPCR NB. The dashed line represents equivalence.

    Techniques Used:

    HIV DNA copies per cell calculated for different 8E5 sources. ( a ) Comparison of 8E5 Standards 1, 2 and 3 analysed by dPCR. ( b ) Effect of culture passage on HIV DNA content per cell for 8E5 Standard 3 measured using the RainDrop ® dPCR platform ( c ) Effect of culture passage on HIV DNA content per cell for 8E5 Standard 3 measured using the QX200™ dPCR platform. Mean values with standard deviations are plotted.
    Figure Legend Snippet: HIV DNA copies per cell calculated for different 8E5 sources. ( a ) Comparison of 8E5 Standards 1, 2 and 3 analysed by dPCR. ( b ) Effect of culture passage on HIV DNA content per cell for 8E5 Standard 3 measured using the RainDrop ® dPCR platform ( c ) Effect of culture passage on HIV DNA content per cell for 8E5 Standard 3 measured using the QX200™ dPCR platform. Mean values with standard deviations are plotted.

    Techniques Used:

    Median HIV DNA copies per million cells (boxplots with interquartile and range) of seven clinical samples assayed in duplicate by qPCR using 8E5 Standards 1, 2 and 3 for calibration. ( a ) Calculated assuming one HIV DNA copy per 8E5 cell for all three Standards. ( b ) Calculated using the actual number of HIV DNA copies per 8E5 cell as determined by dPCR for each of the three Standards.
    Figure Legend Snippet: Median HIV DNA copies per million cells (boxplots with interquartile and range) of seven clinical samples assayed in duplicate by qPCR using 8E5 Standards 1, 2 and 3 for calibration. ( a ) Calculated assuming one HIV DNA copy per 8E5 cell for all three Standards. ( b ) Calculated using the actual number of HIV DNA copies per 8E5 cell as determined by dPCR for each of the three Standards.

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    ATCC 8e5 cell line 8e5a atcc crl 8993tm
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Cell Line 8e5a Atcc Crl 8993tm, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cell line 8e5a atcc crl 8993tm/product/ATCC
    Average 94 stars, based on 1 article reviews
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    ATCC 8e5 crl 8993 cells
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Crl 8993 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 crl 8993 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
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    8e5 crl 8993 cells - by Bioz Stars, 2023-01
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    ATCC 8e5 cell line
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    8e5 cell line - by Bioz Stars, 2023-01
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    ATCC 8e5 lav cell line
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Lav Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hiv env
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    Hiv Env, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC 8e5 cells
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    8e5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cells/product/ATCC
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    94
    ATCC crl 8993
    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the <t>8E5A</t> cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.
    Crl 8993, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

    Journal: Scientific Reports

    Article Title: Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy

    doi: 10.1038/s41598-022-13581-8

    Figure Lengend Snippet: Standard test of the qPCR kit over several years and of different cell lines. The left part of the graph shows the quantification of HIV DNA performed in triplicates by the dPCR thermocycler ThermoFisher on the different batches of the Biocentric qPCR kit, where the y-axis represents HIV DNA copies/cells while the x-axis is the batch numbers and kits expiration dates. The right and shaded part of the graph shows the quantification of HIV DNA performed on three technologically different dPCR thermocyclers: ThermoFisher, Bio-Rad, and Stilla for the 8E5A cell-line and the standard 007; ThermoFisher and Bio-Rad for the 8E5B cell-line, and ThermoFisher for the U1 cell-line.

    Article Snippet: The laboratory’s 8E5 cell line (8E5A)(ATCC CRL-8993TM) was thawed and cultured in RPMI 1640 medium complete with 9% SVF and stored at 37 °C and 5% CO 2 .

    Techniques: