crl  (ATCC)


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    ATCC crl
    Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcc2935 atcc crl 2869 hcc4006 atcc crl 2871 hcc827 atcc crl 2868 nci h1650 atcc crl 5883 nci h3255 atcc  (ATCC)


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    ATCC hcc2935 atcc crl 2869 hcc4006 atcc crl 2871 hcc827 atcc crl 2868 nci h1650 atcc crl 5883 nci h3255 atcc
    Hcc2935 Atcc Crl 2869 Hcc4006 Atcc Crl 2871 Hcc827 Atcc Crl 2868 Nci H1650 Atcc Crl 5883 Nci H3255 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 5883 hcc4006 er cvcl s746 hcc827 gr5 cvcl v622 h1666 atcc  (ATCC)


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    ATCC crl 5883 hcc4006 er cvcl s746 hcc827 gr5 cvcl v622 h1666 atcc
    Crl 5883 Hcc4006 Er Cvcl S746 Hcc827 Gr5 Cvcl V622 H1666 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 5883 nci h1703 atcc  (ATCC)


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    ATCC crl 5883 nci h1703 atcc
    Crl 5883 Nci H1703 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci h1650 crl 5883 cells  (ATCC)


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    ATCC nci h1650 crl 5883 cells
    Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for <t>NCI-H1650,</t> 100 for NCI-H226, and 109 for A549 cells
    Nci H1650 Crl 5883 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Modulation of cellular transcriptome and proteome composition by azidohomoalanine—implications on click chemistry–based secretome analysis"

    Article Title: Modulation of cellular transcriptome and proteome composition by azidohomoalanine—implications on click chemistry–based secretome analysis

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    doi: 10.1007/s00109-023-02333-4

    Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for NCI-H1650, 100 for NCI-H226, and 109 for A549 cells
    Figure Legend Snippet: Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for NCI-H1650, 100 for NCI-H226, and 109 for A549 cells

    Techniques Used: Expressing, Labeling

    crl 5883 h2009 atcc  (ATCC)


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    ATCC crl 5883 h2009 atcc
    Crl 5883 H2009 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcc2935 atcc crl 2869 hcc4006 atcc crl 2871 hcc827 atcc crl 2868 nci h1650 atcc crl 5883 nci h3255 atcc  (ATCC)


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    ATCC hcc2935 atcc crl 2869 hcc4006 atcc crl 2871 hcc827 atcc crl 2868 nci h1650 atcc crl 5883 nci h3255 atcc
    Hcc2935 Atcc Crl 2869 Hcc4006 Atcc Crl 2871 Hcc827 Atcc Crl 2868 Nci H1650 Atcc Crl 5883 Nci H3255 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci h1650 crl 5883 cell lines  (ATCC)


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    ATCC nci h1650 crl 5883 cell lines
    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, <t>NCI-H1650</t> cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.
    Nci H1650 Crl 5883 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Anti-tumor effects of NK cells and anti-PD-L1 antibody with antibody-dependent cellular cytotoxicity in PD-L1-positive cancer cell lines"

    Article Title: Anti-tumor effects of NK cells and anti-PD-L1 antibody with antibody-dependent cellular cytotoxicity in PD-L1-positive cancer cell lines

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2020-000873

    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, NCI-H1650 cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.
    Figure Legend Snippet: Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, NCI-H1650 cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.

    Techniques Used: Release Assay

    nci h1650 crl 5883  (ATCC)


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    ATCC nci h1650 crl 5883
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    crl  (ATCC)


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    ATCC crl
    Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 5883  (ATCC)


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    ATCC crl 5883
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    ATCC crl
    Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hcc2935 atcc crl 2869 hcc4006 atcc crl 2871 hcc827 atcc crl 2868 nci h1650 atcc crl 5883 nci h3255 atcc
    Hcc2935 Atcc Crl 2869 Hcc4006 Atcc Crl 2871 Hcc827 Atcc Crl 2868 Nci H1650 Atcc Crl 5883 Nci H3255 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC crl 5883 hcc4006 er cvcl s746 hcc827 gr5 cvcl v622 h1666 atcc
    Crl 5883 Hcc4006 Er Cvcl S746 Hcc827 Gr5 Cvcl V622 H1666 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC crl 5883 nci h1703 atcc
    Crl 5883 Nci H1703 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci h1650 crl 5883 cells
    Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for <t>NCI-H1650,</t> 100 for NCI-H226, and 109 for A549 cells
    Nci H1650 Crl 5883 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC crl 5883 h2009 atcc
    Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for <t>NCI-H1650,</t> 100 for NCI-H226, and 109 for A549 cells
    Crl 5883 H2009 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci h1650 crl 5883 cell lines
    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, <t>NCI-H1650</t> cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.
    Nci H1650 Crl 5883 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci h1650 crl 5883
    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, <t>NCI-H1650</t> cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.
    Nci H1650 Crl 5883, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC crl 5883
    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, <t>NCI-H1650</t> cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.
    Crl 5883, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for NCI-H1650, 100 for NCI-H226, and 109 for A549 cells

    Journal: Journal of Molecular Medicine (Berlin, Germany)

    Article Title: Modulation of cellular transcriptome and proteome composition by azidohomoalanine—implications on click chemistry–based secretome analysis

    doi: 10.1007/s00109-023-02333-4

    Figure Lengend Snippet: Heatmaps showing the number of secreted proteins with regulated gene and protein expression profile induced by AHA labeling. a Distribution of secreted proteins from MC38 cells with regard to their regulation in the transcriptome and proteome expression profile. The colors indicate the number of proteins present in the secretome that show an up-, down-, or not regulated profile in the transcriptome and proteome analysis. The total number of proteins identified in the secretome, which were also detected in transcriptome and proteome analysis, was 388. b Analysis as in a for the secretome of the six human tumor cell lines. The total number of proteins identified in the secretome and also in transcriptome and proteome analysis was 329 for MEL-HO cells, 299 for SK-MEL-37 cells, 327 for D41-MEL, 37 for NCI-H1650, 100 for NCI-H226, and 109 for A549 cells

    Article Snippet: MC38 cells were kindly provided by H.-C. Probst (Institute for Immunology, University Medical Center, Johannes Gutenberg University Mainz), NCI-H226 (CRL-5826) and NCI-H1650 (CRL-5883) cells were obtained from ATCC, A549 (ACC 107) and MEL-HO (ACC 62) cells were obtained from the DSMZ-German Collection of Microorganisms and Cell Culture GmbH, D41-MEL cells were kindly provided by Catherine Wölfel (Department of Hematology and Oncology, University Medical Center, Johannes Gutenberg University Mainz), Jurkat cells were kindly provided by Ari Waisman (Institute for Molecular Medicine, University Medical Center, Johannes Gutenberg University Mainz), and SK-MEL-37 cells were obtained from Sigma-Aldrich (SCC262).

    Techniques: Expressing, Labeling

    Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, NCI-H1650 cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Anti-tumor effects of NK cells and anti-PD-L1 antibody with antibody-dependent cellular cytotoxicity in PD-L1-positive cancer cell lines

    doi: 10.1136/jitc-2020-000873

    Figure Lengend Snippet: Anti-PD-L1 mAbs-mediated ADCC in human cancer cell lines. NK-92-CD16 cytotoxicity against tumor cells was measured by a standard 51 Cr-release assay with various E:T ratios (30:1, 10:1, and 3:1). Bar graph represents cytotoxicity of NK-92-CD16 cells against cancer cell lines at 30:1 E:T ratio. All cancer cell lines were treated with 10 µg/mL of IgG1 isotype control (black dotted lines and bar), atezolizumab (green), IMC-001 (red), and anti-hPD-L1-hIgG1 (blue). (A) Head and neck squamous cell carcinoma cell lines. PD-L1 low (left) includes SNU-1041, SNU-1066, and Detroit 562, and PD-L1 high (right) includes SNU-1076, FaDu, and HN31 cells. (B) For non-small-cell lung cancer, NCI-H1650 cells are PD-L1 low (left), and NCI-H1975 cells are PD-L1 high (right). All experiments were performed three times independently. Statistical significance across groups 6 was determined by one-way analysis of variance. All data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ADCC, antibody-dependent cellular cytotoxicity; E:T, effector-to-target; mAb, monoclonal antibody; NK, natural killer; ns, not significant. PD-L1; programmed death-ligand 1.

    Article Snippet: Detroit 562 (CCL-138), FaDu (HTB-43), NCI-H1975 (CRL-5908), and NCI-H1650 (CRL-5883) cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Release Assay