human gastric cancer cells nci n87  (ATCC)


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    ATCC human gastric cancer cells nci n87
    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Human Gastric Cancer Cells Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review"

    Article Title: The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review

    Journal: Foods

    doi: 10.3390/foods12020415

    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Figure Legend Snippet: Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.

    Techniques Used: In Vitro, In Vivo, Activity Assay, Antioxidant Assay, Positive Control, Antioxidant Activity Assay, CCK-8 Assay, Permeability, Flow Cytometry, Western Blot, Microscopy, Activation Assay, Expressing, Staining, Immunohistochemistry, Cell Culture, Concentration Assay, TUNEL Assay, Mouse Assay

    nci n87 crl 5822 cell line  (ATCC)


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    ATCC nci n87 crl 5822 cell line
    Nci N87 Crl 5822 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci n87 crl 5822 cell line  (ATCC)


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    ATCC nci n87 crl 5822 cell line
    Nci N87 Crl 5822 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human gastric cancer cells nci n87  (ATCC)


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    ATCC human gastric cancer cells nci n87
    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Human Gastric Cancer Cells Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review"

    Article Title: The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review

    Journal: Foods

    doi: 10.3390/foods12020415

    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Figure Legend Snippet: Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.

    Techniques Used: In Vitro, In Vivo, Activity Assay, Antioxidant Assay, Positive Control, Antioxidant Activity Assay, CCK-8 Assay, Permeability, Flow Cytometry, Western Blot, Microscopy, Activation Assay, Expressing, Staining, Immunohistochemistry, Cell Culture, Concentration Assay, TUNEL Assay, Mouse Assay

    nci n87  (ATCC)


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    ATCC nci n87
    IC 50 values of C3G and DDP in GC cells.
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Cyanidin-3-O-Glucoside Induces the Apoptosis of Human Gastric Cancer MKN-45 Cells through ROS-Mediated Signaling Pathways"

    Article Title: Cyanidin-3-O-Glucoside Induces the Apoptosis of Human Gastric Cancer MKN-45 Cells through ROS-Mediated Signaling Pathways

    Journal: Molecules

    doi: 10.3390/molecules28020652

    IC 50 values of C3G and DDP in GC cells.
    Figure Legend Snippet: IC 50 values of C3G and DDP in GC cells.

    Techniques Used:

    crl 5822  (ATCC)


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    ATCC crl 5822
    Crl 5822, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci n87 cell lines  (ATCC)


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    ATCC nci n87 cell lines
    (a) HER2-overexpressing <t>NCI-N87</t> tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).
    Nci N87 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "FAP-retargeted Ad5 enables in vivo gene delivery to stromal cells in the tumor microenvironment"

    Article Title: FAP-retargeted Ad5 enables in vivo gene delivery to stromal cells in the tumor microenvironment

    Journal: bioRxiv

    doi: 10.1101/2022.12.19.520931

    (a) HER2-overexpressing NCI-N87 tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).
    Figure Legend Snippet: (a) HER2-overexpressing NCI-N87 tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).

    Techniques Used: Labeling, Expressing, Injection, Flow Cytometry, Marker, Staining, Immunohistochemical staining, Immunofluorescence, Transduction

    (a) Growth analysis of HER2 + tumor xenografts upon Ad5-mediated treatment with trastuzumab (TZB) or with TZB as a protein. HER2-overexpressing NCI-N87 tumor cells and NIH3T3mFAP cells were co-injected subcutaneously into the flank of SCID/beige mice for tumor establishment. At a tumor volume of 50 mm 3 , mice were treated intratumorally with 9×10 8 PFU FAP-retargeted Ad5 encoding trastuzumab (FAP-Ad5-TZB; n = 5), or one single dose of 200 µg Herceptin (n = 3), or three doses of 200 µg Herceptin (n = 3), or PBS (n = 5). Arrows indicate time points of injection for the corresponding treatment. Data points represent mean ± SD. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005, ***p < 0.0005. (b) Tumor weights of harvested tumors from (a) 19 days post injection. Each data point represents a single mouse. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005. (c) Detection of TZB within harvested tumors from (a) 19 days post injection. Representative immunofluorescence images of tumor tissues stained for TZB (cyan) and counter-stained with DAPI (blue) for nuclei staining.
    Figure Legend Snippet: (a) Growth analysis of HER2 + tumor xenografts upon Ad5-mediated treatment with trastuzumab (TZB) or with TZB as a protein. HER2-overexpressing NCI-N87 tumor cells and NIH3T3mFAP cells were co-injected subcutaneously into the flank of SCID/beige mice for tumor establishment. At a tumor volume of 50 mm 3 , mice were treated intratumorally with 9×10 8 PFU FAP-retargeted Ad5 encoding trastuzumab (FAP-Ad5-TZB; n = 5), or one single dose of 200 µg Herceptin (n = 3), or three doses of 200 µg Herceptin (n = 3), or PBS (n = 5). Arrows indicate time points of injection for the corresponding treatment. Data points represent mean ± SD. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005, ***p < 0.0005. (b) Tumor weights of harvested tumors from (a) 19 days post injection. Each data point represents a single mouse. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005. (c) Detection of TZB within harvested tumors from (a) 19 days post injection. Representative immunofluorescence images of tumor tissues stained for TZB (cyan) and counter-stained with DAPI (blue) for nuclei staining.

    Techniques Used: Injection, Immunofluorescence, Staining

    nci n87  (ATCC)


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    ATCC nci n87
    (A) Total cell extracts of gastric cancer cells were analyzed by immunoblotting for expression of stathmin and phospho-stathmin. (B–D) Human gastric cancer cells SNU16(B), <t>NCI-N87</t> (C) and AGS (D) were plated on 96-well plates and treated with 1 nM to 1000 nM concentrations of nab-paclitaxel, 5-fluorouracil, oxaliplatin and epirubicin. After 72 hours, 10 μl WST-1 reagent was added in each well and incubated for 2 additional hours. The absorbance at 450 nm was measured using a microplate reader. The resulting number of viable cells was calculated by measuring absorbance of color produced in each well. Data are the mean ± SD of triplicate determinations.
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Superior Antitumor Activity of Nanoparticle Albumin-Bound Paclitaxel in Experimental Gastric Cancer"

    Article Title: Superior Antitumor Activity of Nanoparticle Albumin-Bound Paclitaxel in Experimental Gastric Cancer

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0058037

    (A) Total cell extracts of gastric cancer cells were analyzed by immunoblotting for expression of stathmin and phospho-stathmin. (B–D) Human gastric cancer cells SNU16(B), NCI-N87 (C) and AGS (D) were plated on 96-well plates and treated with 1 nM to 1000 nM concentrations of nab-paclitaxel, 5-fluorouracil, oxaliplatin and epirubicin. After 72 hours, 10 μl WST-1 reagent was added in each well and incubated for 2 additional hours. The absorbance at 450 nm was measured using a microplate reader. The resulting number of viable cells was calculated by measuring absorbance of color produced in each well. Data are the mean ± SD of triplicate determinations.
    Figure Legend Snippet: (A) Total cell extracts of gastric cancer cells were analyzed by immunoblotting for expression of stathmin and phospho-stathmin. (B–D) Human gastric cancer cells SNU16(B), NCI-N87 (C) and AGS (D) were plated on 96-well plates and treated with 1 nM to 1000 nM concentrations of nab-paclitaxel, 5-fluorouracil, oxaliplatin and epirubicin. After 72 hours, 10 μl WST-1 reagent was added in each well and incubated for 2 additional hours. The absorbance at 450 nm was measured using a microplate reader. The resulting number of viable cells was calculated by measuring absorbance of color produced in each well. Data are the mean ± SD of triplicate determinations.

    Techniques Used: Western Blot, Expressing, Incubation, Produced

    (A) SNU16 cells were cultured for 24 hours and then treated with 100 nM nab-paclitaxel for 12 hours. Cell cycle analysis was performed by flow cytometry. Results shown are representative of two independent experiments. (B–D) Expression of phospho-stathmin and mitotic cell death was evaluated in SNU16 (B), NCI -N87 (C), and AGS (C) cells. Cultured cells were treated with nab-paclitaxel (10 μM) and showed increased mitotic arrest configuration and phospho-stathmin expression by immunocytochemical staining. Mitotic arrests were detected in the presence of phospho-stathmin expression. (D) A sub-confluent monolayer of human gastric cancer cells AGS, NCI-N87 and SNU16 was treated with nab-paclitaxel (10 μM) for 3, 6 and 9 hours and analyzed by immunoblotting for phospho-stathmin and total stathmin. (E) Human gastric cancer cell were treated with nab-paclitaxel (10 μM), oxaliplatin (10 μM), and epirubicin (10 μM) for 16 hours and analyzed for cleaved PARP-1 and caspase-3. Data are representative of two independent experiments with similar results.
    Figure Legend Snippet: (A) SNU16 cells were cultured for 24 hours and then treated with 100 nM nab-paclitaxel for 12 hours. Cell cycle analysis was performed by flow cytometry. Results shown are representative of two independent experiments. (B–D) Expression of phospho-stathmin and mitotic cell death was evaluated in SNU16 (B), NCI -N87 (C), and AGS (C) cells. Cultured cells were treated with nab-paclitaxel (10 μM) and showed increased mitotic arrest configuration and phospho-stathmin expression by immunocytochemical staining. Mitotic arrests were detected in the presence of phospho-stathmin expression. (D) A sub-confluent monolayer of human gastric cancer cells AGS, NCI-N87 and SNU16 was treated with nab-paclitaxel (10 μM) for 3, 6 and 9 hours and analyzed by immunoblotting for phospho-stathmin and total stathmin. (E) Human gastric cancer cell were treated with nab-paclitaxel (10 μM), oxaliplatin (10 μM), and epirubicin (10 μM) for 16 hours and analyzed for cleaved PARP-1 and caspase-3. Data are representative of two independent experiments with similar results.

    Techniques Used: Cell Culture, Cell Cycle Assay, Flow Cytometry, Expressing, Staining, Western Blot

    (A) SCID mice were subcutaneously injected with SNU16 cells (20×10 6 ) and treated with nab-paclitaxel, oxaliplatin and epirubicin for 2 weeks. (B) SCID mice were subcutaneously injected with NCI-N87 cells (10×10 6 ) and treated with nab-paclitaxel for 2 weeks. Relative tumor volume and tumor weight on the final day were assessed. Data are representative of mean values ± standard deviation from 6–8 mice per group. Symbol * represents significant difference (p<0.05) and symbol ** represents significant differences (p<0.001) compared to vehicle controls; ns = no significant difference.
    Figure Legend Snippet: (A) SCID mice were subcutaneously injected with SNU16 cells (20×10 6 ) and treated with nab-paclitaxel, oxaliplatin and epirubicin for 2 weeks. (B) SCID mice were subcutaneously injected with NCI-N87 cells (10×10 6 ) and treated with nab-paclitaxel for 2 weeks. Relative tumor volume and tumor weight on the final day were assessed. Data are representative of mean values ± standard deviation from 6–8 mice per group. Symbol * represents significant difference (p<0.05) and symbol ** represents significant differences (p<0.001) compared to vehicle controls; ns = no significant difference.

    Techniques Used: Injection, Standard Deviation

    SCID mice were subcutaneously injected SNU16 cells (20×10 6 ) or NCI-N87 cells (10×10 6 ) and treated with nab-paclitaxel for 2 weeks. Intratumoral expression of phospho-stathmin was measured in SNU16 (A) or NCI-N87 (B) tumor tissue by immunostaining tissue sections for phospho-stathmin (ser38) antigen and photographed under a fluorescent microscope. Tumor lysates were prepared from tumor tissue samples obtained from SNU16 (A) or NCI-N87 (B) tumor bearing SCID mice after nab-paclitaxel therapy and then analyzed by immunoblotting for phospho-stathmin and total stathmin.
    Figure Legend Snippet: SCID mice were subcutaneously injected SNU16 cells (20×10 6 ) or NCI-N87 cells (10×10 6 ) and treated with nab-paclitaxel for 2 weeks. Intratumoral expression of phospho-stathmin was measured in SNU16 (A) or NCI-N87 (B) tumor tissue by immunostaining tissue sections for phospho-stathmin (ser38) antigen and photographed under a fluorescent microscope. Tumor lysates were prepared from tumor tissue samples obtained from SNU16 (A) or NCI-N87 (B) tumor bearing SCID mice after nab-paclitaxel therapy and then analyzed by immunoblotting for phospho-stathmin and total stathmin.

    Techniques Used: Injection, Expressing, Immunostaining, Microscopy, Western Blot

    nci n87 cells  (ATCC)


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    ATCC nci n87 cells
    (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and <t>NCI-N87</t> cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.
    Nci N87 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "VEZT, a Novel Putative Tumor Suppressor, Suppresses the Growth and Tumorigenicity of Gastric Cancer"

    Article Title: VEZT, a Novel Putative Tumor Suppressor, Suppresses the Growth and Tumorigenicity of Gastric Cancer

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074409

    (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and NCI-N87 cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.
    Figure Legend Snippet: (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and NCI-N87 cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.

    Techniques Used: Expressing, Plasmid Preparation, Transfection, Over Expression, CCK-8 Assay, Standard Deviation

    nci n87  (ATCC)


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    ATCC nci n87
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci n87  (ATCC)


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    ATCC nci n87
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human gastric cancer cells nci n87
    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Human Gastric Cancer Cells Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci n87 crl 5822 cell line
    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.
    Nci N87 Crl 5822 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci n87
    IC 50 values of C3G and DDP in GC cells.
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC crl 5822
    IC 50 values of C3G and DDP in GC cells.
    Crl 5822, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci n87 cell lines
    (a) HER2-overexpressing <t>NCI-N87</t> tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).
    Nci N87 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci n87 cells
    (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and <t>NCI-N87</t> cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.
    Nci N87 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.

    Journal: Foods

    Article Title: The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review

    doi: 10.3390/foods12020415

    Figure Lengend Snippet: Reports of in vitro and in vivo effects of propolis from studies on gastric cancer performed in different countries.

    Article Snippet: New Zealand , -CAPE -Pinobanksin -Pinobanksin-3-O-acetate -Pinocembrin -Chrysin -Galangin , Model: Human gastric cancer cells NCI-N87 (ATCC CRL-5822). Protocol: Production of different types of propolis-cyclodextrin complexes: CD1, CD2, CD3, CD4 and CD5. In vitro cytotoxic activity was assessed by the MTT method. Activated neutrophil anti-inflammatory assays. Lipid antioxidant assay. Positive control 5-FU tested at 15 ng/mL. Compounds reported in propolis were given by the manufacturer of this sample. , -Cytotoxic activity: Propolis complexes had moderate cytotoxic activity since CD3 inhibited NCI-N87 cells by 32.7%, CD4 by 24.6%, and CD5 by 21.8% at 200 μg/mL. Pinocembrin had 72.5% cytotoxic activity at 200 μg/mL. -Anti-inflammatory activity: At 50 μg/mL, New Zealand propolis (alone) inhibited TNF-α by 85% ± 1, CD1 by 93% ± 1, and CD2 by 97% ± 1. At 200 μg/mL, all three samples inhibited this cytokine by 100%. -Lipid antioxidant activity: The five propolis complexes and CAPE (also in the γ-CD complex) had moderate antioxidant activity. CAPE (alone) showed strong antioxidant activity. , [ ] .

    Techniques: In Vitro, In Vivo, Activity Assay, Antioxidant Assay, Positive Control, Antioxidant Activity Assay, CCK-8 Assay, Permeability, Flow Cytometry, Western Blot, Microscopy, Activation Assay, Expressing, Staining, Immunohistochemistry, Cell Culture, Concentration Assay, TUNEL Assay, Mouse Assay

    IC 50 values of C3G and DDP in GC cells.

    Journal: Molecules

    Article Title: Cyanidin-3-O-Glucoside Induces the Apoptosis of Human Gastric Cancer MKN-45 Cells through ROS-Mediated Signaling Pathways

    doi: 10.3390/molecules28020652

    Figure Lengend Snippet: IC 50 values of C3G and DDP in GC cells.

    Article Snippet: AGS, MKN-45, HGC-27, SGC-7901, MKN-74, BGC-823, MKN-28, KATO-3, YCC-1, NCI-N87, MGC-803, and YCC-6 cells were from the American Type Culture Collection (Manassas, VA, USA).

    Techniques:

    (a) HER2-overexpressing NCI-N87 tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).

    Journal: bioRxiv

    Article Title: FAP-retargeted Ad5 enables in vivo gene delivery to stromal cells in the tumor microenvironment

    doi: 10.1101/2022.12.19.520931

    Figure Lengend Snippet: (a) HER2-overexpressing NCI-N87 tumor cells and GFP-labeled, mFAP-expressing NIH3T3mFAP fibroblast cells were co-injected subcutaneously into the flank of SCID/beige mice. After tumor establishment (200 mm 3 tumor volume), mice were treated intratumorally with 3×10 9 PFU FAP-retargeted or untargeted Ad5 encoding TdTomato. Three days post injection, tumors were harvested and analyzed by flow cytometry. Transduced cells were detected via TdTomato expression and further characterized by cell surface marker staining or GFP-expression. Each data point represents a single mouse. Bars represent mean ± SD of five mice per group. Statistics: Unpaired t-test; *p < 0.05. Representative data of two independent experiments are shown. (b) Quantification of (a), indicating mean values of transduced cells. (c) Immunohistochemical analysis of (a) to investigate the cell-specificity of FAP-retargeted Ad5. Representative immunofluorescence images of tumor tissues stained for HER2 (cyan) and counter-stained with DAPI (blue) for nuclei staining. FAP + cells were detected via GFP-expression (green), and cells transduced with Ad5 were detected via TdTomato expression (magenta).

    Article Snippet: HEK293, D551, HeLa, A549, and NCI-N87 cell lines were purchased from the American Type Culture Collection (ATCC).

    Techniques: Labeling, Expressing, Injection, Flow Cytometry, Marker, Staining, Immunohistochemical staining, Immunofluorescence, Transduction

    (a) Growth analysis of HER2 + tumor xenografts upon Ad5-mediated treatment with trastuzumab (TZB) or with TZB as a protein. HER2-overexpressing NCI-N87 tumor cells and NIH3T3mFAP cells were co-injected subcutaneously into the flank of SCID/beige mice for tumor establishment. At a tumor volume of 50 mm 3 , mice were treated intratumorally with 9×10 8 PFU FAP-retargeted Ad5 encoding trastuzumab (FAP-Ad5-TZB; n = 5), or one single dose of 200 µg Herceptin (n = 3), or three doses of 200 µg Herceptin (n = 3), or PBS (n = 5). Arrows indicate time points of injection for the corresponding treatment. Data points represent mean ± SD. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005, ***p < 0.0005. (b) Tumor weights of harvested tumors from (a) 19 days post injection. Each data point represents a single mouse. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005. (c) Detection of TZB within harvested tumors from (a) 19 days post injection. Representative immunofluorescence images of tumor tissues stained for TZB (cyan) and counter-stained with DAPI (blue) for nuclei staining.

    Journal: bioRxiv

    Article Title: FAP-retargeted Ad5 enables in vivo gene delivery to stromal cells in the tumor microenvironment

    doi: 10.1101/2022.12.19.520931

    Figure Lengend Snippet: (a) Growth analysis of HER2 + tumor xenografts upon Ad5-mediated treatment with trastuzumab (TZB) or with TZB as a protein. HER2-overexpressing NCI-N87 tumor cells and NIH3T3mFAP cells were co-injected subcutaneously into the flank of SCID/beige mice for tumor establishment. At a tumor volume of 50 mm 3 , mice were treated intratumorally with 9×10 8 PFU FAP-retargeted Ad5 encoding trastuzumab (FAP-Ad5-TZB; n = 5), or one single dose of 200 µg Herceptin (n = 3), or three doses of 200 µg Herceptin (n = 3), or PBS (n = 5). Arrows indicate time points of injection for the corresponding treatment. Data points represent mean ± SD. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005, ***p < 0.0005. (b) Tumor weights of harvested tumors from (a) 19 days post injection. Each data point represents a single mouse. Statistics: Unpaired t-test; *p < 0.05, **p < 0.005. (c) Detection of TZB within harvested tumors from (a) 19 days post injection. Representative immunofluorescence images of tumor tissues stained for TZB (cyan) and counter-stained with DAPI (blue) for nuclei staining.

    Article Snippet: HEK293, D551, HeLa, A549, and NCI-N87 cell lines were purchased from the American Type Culture Collection (ATCC).

    Techniques: Injection, Immunofluorescence, Staining

    (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and NCI-N87 cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.

    Journal: PLoS ONE

    Article Title: VEZT, a Novel Putative Tumor Suppressor, Suppresses the Growth and Tumorigenicity of Gastric Cancer

    doi: 10.1371/journal.pone.0074409

    Figure Lengend Snippet: (A) The different expression levels of VEZT in five gastric cancer cell lines and one immortalized gastric mucosal cell line. (B) Expression of VEZT was upregulated in MKN-45 and NCI-N87 cells upon VEZT vector transfection relative to N1-controls. (C) Invasive, migratory and tubular formation capacities of VEZT-transfected MKN-45 and NCI-N87 cells were suppressed as determined by the transwell and tubular formation assays. (D) Overexpression of VEZT leads to cell growth arrest as determined by the CCK-8 assay. (E) Colony formation rates were significantly different between VEZT-transfected cells and N1-controls in MKN-45 and NCI-N87 cells. (F) The overexpression of VEZT in MKN-45 and NCI-N87 cells inhibited tumorigenesis in nude mice. Tumor nodules resected from the VEZT-transfected group were smaller than those from N1-controls. (G) Tumor growth curves for the VEZT/N1 group and N1-controls show rapid tumor growth in the MKN-45 or NCI-N87/N1control groups. * P < 0.05. Each bar represents the mean value ± standard deviation from three independent experiments.

    Article Snippet: Gastric cancer cell lines SNU-1 and NCI-N87 cells were obtained from American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Plasmid Preparation, Transfection, Over Expression, CCK-8 Assay, Standard Deviation