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nci n87  (ATCC)


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    Structured Review

    ATCC nci n87
    Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) <t>NCI-N87</t> and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.
    Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nci n87/product/ATCC
    Average 97 stars, based on 1 article reviews
    nci n87 - by Bioz Stars, 2025-02
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    Images

    1) Product Images from "Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling"

    Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2024.13400

    Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) NCI-N87 and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.
    Figure Legend Snippet: Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) NCI-N87 and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.

    Techniques Used: Cell Counting, Staining, Control

    Solamargine significantly reduces the migration and invasion of gastric cancer cells. (A) Migration and (B) invasion of NCI-N87 and HGC-27 cells were investigated using Transwell assays. Magnification, ×200. **P<0.01 vs. Control.
    Figure Legend Snippet: Solamargine significantly reduces the migration and invasion of gastric cancer cells. (A) Migration and (B) invasion of NCI-N87 and HGC-27 cells were investigated using Transwell assays. Magnification, ×200. **P<0.01 vs. Control.

    Techniques Used: Migration, Control

    Solamargine reverses the IL-6-induced activation of STAT3/PD-L1 signaling. GC cells were pretreated with 20 ng/ml IL-6 for 24 h, before being co-cultured with Jurkat-T cells for 48 h. Subsequently, GC cells were collected for further analysis. The protein expression levels of c-Myc and PD-L1 in (A) HGC-27 and (B) NCI-N87 cells were assessed using western blotting. Protein levels of p-STAT3 (normalized to STAT3) in (C) HGC-27 and (D) NCI-N87 cells were investigated using immunofluorescence staining. Magnification, ×200. **P<0.01 vs. Control. # P<0.05 and ## P<0.01 vs. IL-6 alone. p-, phosphorylated; PD-L1, programmed cell death ligand 1; GC, gastric cancer.
    Figure Legend Snippet: Solamargine reverses the IL-6-induced activation of STAT3/PD-L1 signaling. GC cells were pretreated with 20 ng/ml IL-6 for 24 h, before being co-cultured with Jurkat-T cells for 48 h. Subsequently, GC cells were collected for further analysis. The protein expression levels of c-Myc and PD-L1 in (A) HGC-27 and (B) NCI-N87 cells were assessed using western blotting. Protein levels of p-STAT3 (normalized to STAT3) in (C) HGC-27 and (D) NCI-N87 cells were investigated using immunofluorescence staining. Magnification, ×200. **P<0.01 vs. Control. # P<0.05 and ## P<0.01 vs. IL-6 alone. p-, phosphorylated; PD-L1, programmed cell death ligand 1; GC, gastric cancer.

    Techniques Used: Activation Assay, Cell Culture, Expressing, Western Blot, Immunofluorescence, Staining, Control

    Solamargine activation of T cells. Jurkat T cells were activated using stimulation with anti-CD3/CD28 antibodies and then co-cultured with non-treated or 10 µM solamargine pretreated NCI-N87 or HGC-27 cells. Subsequently, the Jurkat T cells were collected. (A) Representative flow cytometry histograms and (B) the MFI of CD69 in Jurka T cells assessed using flow cytometry. **P<0.01 vs.(−) Jurkat T cell only. ## P<0.01 vs. NCI-N87 control. ^^ P<0.01 vs. HGC-27 control. MFI, mean fluorescence intensity. (−) Jurkat T cell indicates Jurkat T cell without CD3/CD28 stimulation.
    Figure Legend Snippet: Solamargine activation of T cells. Jurkat T cells were activated using stimulation with anti-CD3/CD28 antibodies and then co-cultured with non-treated or 10 µM solamargine pretreated NCI-N87 or HGC-27 cells. Subsequently, the Jurkat T cells were collected. (A) Representative flow cytometry histograms and (B) the MFI of CD69 in Jurka T cells assessed using flow cytometry. **P<0.01 vs.(−) Jurkat T cell only. ## P<0.01 vs. NCI-N87 control. ^^ P<0.01 vs. HGC-27 control. MFI, mean fluorescence intensity. (−) Jurkat T cell indicates Jurkat T cell without CD3/CD28 stimulation.

    Techniques Used: Activation Assay, Cell Culture, Flow Cytometry, Control, Fluorescence



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    ncin87  (ATCC)
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    Luteoloside inhibits the proliferation, invasion and migration of human gastric cancer <t>NCI-N87</t> cells. (A) Cell Counting Kit-8 assay using a microplate reader in the final step to measure the OD 450 of each well as an indicator of cell proliferation. (B) Transwell assay to determine the effect of luteoloside treatment on NCI-N87-cell invasion (scale bars, 50 µm). (C) Scratch-wound assay to determine the migration ability of NCI-N87 cells (scale bars, 100 µm). *P<0.05, **P<0.01 vs. the control group. OD 450 , optical density at 450 nm.
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    Image Search Results


    Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) NCI-N87 and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.

    Journal: Molecular Medicine Reports

    Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling

    doi: 10.3892/mmr.2024.13400

    Figure Lengend Snippet: Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) NCI-N87 and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.

    Article Snippet: GC cell lines, NCI-N87 and HGC-27, and Jurkat T cells were purchased from the American Type Culture Collection.

    Techniques: Cell Counting, Staining, Control

    Solamargine significantly reduces the migration and invasion of gastric cancer cells. (A) Migration and (B) invasion of NCI-N87 and HGC-27 cells were investigated using Transwell assays. Magnification, ×200. **P<0.01 vs. Control.

    Journal: Molecular Medicine Reports

    Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling

    doi: 10.3892/mmr.2024.13400

    Figure Lengend Snippet: Solamargine significantly reduces the migration and invasion of gastric cancer cells. (A) Migration and (B) invasion of NCI-N87 and HGC-27 cells were investigated using Transwell assays. Magnification, ×200. **P<0.01 vs. Control.

    Article Snippet: GC cell lines, NCI-N87 and HGC-27, and Jurkat T cells were purchased from the American Type Culture Collection.

    Techniques: Migration, Control

    Solamargine reverses the IL-6-induced activation of STAT3/PD-L1 signaling. GC cells were pretreated with 20 ng/ml IL-6 for 24 h, before being co-cultured with Jurkat-T cells for 48 h. Subsequently, GC cells were collected for further analysis. The protein expression levels of c-Myc and PD-L1 in (A) HGC-27 and (B) NCI-N87 cells were assessed using western blotting. Protein levels of p-STAT3 (normalized to STAT3) in (C) HGC-27 and (D) NCI-N87 cells were investigated using immunofluorescence staining. Magnification, ×200. **P<0.01 vs. Control. # P<0.05 and ## P<0.01 vs. IL-6 alone. p-, phosphorylated; PD-L1, programmed cell death ligand 1; GC, gastric cancer.

    Journal: Molecular Medicine Reports

    Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling

    doi: 10.3892/mmr.2024.13400

    Figure Lengend Snippet: Solamargine reverses the IL-6-induced activation of STAT3/PD-L1 signaling. GC cells were pretreated with 20 ng/ml IL-6 for 24 h, before being co-cultured with Jurkat-T cells for 48 h. Subsequently, GC cells were collected for further analysis. The protein expression levels of c-Myc and PD-L1 in (A) HGC-27 and (B) NCI-N87 cells were assessed using western blotting. Protein levels of p-STAT3 (normalized to STAT3) in (C) HGC-27 and (D) NCI-N87 cells were investigated using immunofluorescence staining. Magnification, ×200. **P<0.01 vs. Control. # P<0.05 and ## P<0.01 vs. IL-6 alone. p-, phosphorylated; PD-L1, programmed cell death ligand 1; GC, gastric cancer.

    Article Snippet: GC cell lines, NCI-N87 and HGC-27, and Jurkat T cells were purchased from the American Type Culture Collection.

    Techniques: Activation Assay, Cell Culture, Expressing, Western Blot, Immunofluorescence, Staining, Control

    Solamargine activation of T cells. Jurkat T cells were activated using stimulation with anti-CD3/CD28 antibodies and then co-cultured with non-treated or 10 µM solamargine pretreated NCI-N87 or HGC-27 cells. Subsequently, the Jurkat T cells were collected. (A) Representative flow cytometry histograms and (B) the MFI of CD69 in Jurka T cells assessed using flow cytometry. **P<0.01 vs.(−) Jurkat T cell only. ## P<0.01 vs. NCI-N87 control. ^^ P<0.01 vs. HGC-27 control. MFI, mean fluorescence intensity. (−) Jurkat T cell indicates Jurkat T cell without CD3/CD28 stimulation.

    Journal: Molecular Medicine Reports

    Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling

    doi: 10.3892/mmr.2024.13400

    Figure Lengend Snippet: Solamargine activation of T cells. Jurkat T cells were activated using stimulation with anti-CD3/CD28 antibodies and then co-cultured with non-treated or 10 µM solamargine pretreated NCI-N87 or HGC-27 cells. Subsequently, the Jurkat T cells were collected. (A) Representative flow cytometry histograms and (B) the MFI of CD69 in Jurka T cells assessed using flow cytometry. **P<0.01 vs.(−) Jurkat T cell only. ## P<0.01 vs. NCI-N87 control. ^^ P<0.01 vs. HGC-27 control. MFI, mean fluorescence intensity. (−) Jurkat T cell indicates Jurkat T cell without CD3/CD28 stimulation.

    Article Snippet: GC cell lines, NCI-N87 and HGC-27, and Jurkat T cells were purchased from the American Type Culture Collection.

    Techniques: Activation Assay, Cell Culture, Flow Cytometry, Control, Fluorescence

    Luteoloside inhibits the proliferation, invasion and migration of human gastric cancer NCI-N87 cells. (A) Cell Counting Kit-8 assay using a microplate reader in the final step to measure the OD 450 of each well as an indicator of cell proliferation. (B) Transwell assay to determine the effect of luteoloside treatment on NCI-N87-cell invasion (scale bars, 50 µm). (C) Scratch-wound assay to determine the migration ability of NCI-N87 cells (scale bars, 100 µm). *P<0.05, **P<0.01 vs. the control group. OD 450 , optical density at 450 nm.

    Journal: Oncology Letters

    Article Title: Network pharmacology‑based analysis and in vitro experimental verification of the inhibitory role of luteoloside on gastric cancer cells via the p53/p21 pathway

    doi: 10.3892/ol.2024.14822

    Figure Lengend Snippet: Luteoloside inhibits the proliferation, invasion and migration of human gastric cancer NCI-N87 cells. (A) Cell Counting Kit-8 assay using a microplate reader in the final step to measure the OD 450 of each well as an indicator of cell proliferation. (B) Transwell assay to determine the effect of luteoloside treatment on NCI-N87-cell invasion (scale bars, 50 µm). (C) Scratch-wound assay to determine the migration ability of NCI-N87 cells (scale bars, 100 µm). *P<0.05, **P<0.01 vs. the control group. OD 450 , optical density at 450 nm.

    Article Snippet: The human GC cell line NCI-N87 (CRL-5822) was purchased from the American Type Culture Collection and incubated in RPMI-1640 medium (cat. no. R8758; Sigma-Aldrich; Merck KGaA) with 10% fetal bovine serum (cat. no. 16000-044; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Migration, Cell Counting, Transwell Assay, Scratch Wound Assay Assay, Control

    Luteoloside promotes DNA damage of human gastric cancer NCI-N87 cells. (A) Representative comet analysis images and quantification of comet tail DNA levels and tail moment using the OpenComet plugin for ImageJ (scale bars, 20 µm). (B) Effect of luteoloside on γH2AX protein levels in NCI-N87 cells determined by western blot analysis. **P<0.01 vs. the control group.

    Journal: Oncology Letters

    Article Title: Network pharmacology‑based analysis and in vitro experimental verification of the inhibitory role of luteoloside on gastric cancer cells via the p53/p21 pathway

    doi: 10.3892/ol.2024.14822

    Figure Lengend Snippet: Luteoloside promotes DNA damage of human gastric cancer NCI-N87 cells. (A) Representative comet analysis images and quantification of comet tail DNA levels and tail moment using the OpenComet plugin for ImageJ (scale bars, 20 µm). (B) Effect of luteoloside on γH2AX protein levels in NCI-N87 cells determined by western blot analysis. **P<0.01 vs. the control group.

    Article Snippet: The human GC cell line NCI-N87 (CRL-5822) was purchased from the American Type Culture Collection and incubated in RPMI-1640 medium (cat. no. R8758; Sigma-Aldrich; Merck KGaA) with 10% fetal bovine serum (cat. no. 16000-044; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Western Blot, Control

    Luteoloside activates the p53/p21 pathway in human gastric cancer NCI-N87 cells. (A) Western blot analysis was performed to detect the relative expression levels of p53, p21 and Bcl-2 in NCI-N87 cells in each group; (B) quantified expression levels of p53; (C) quantified expression levels of p21; (D) quantified expression levels of Bcl-2. **P<0.01 vs. the control group.

    Journal: Oncology Letters

    Article Title: Network pharmacology‑based analysis and in vitro experimental verification of the inhibitory role of luteoloside on gastric cancer cells via the p53/p21 pathway

    doi: 10.3892/ol.2024.14822

    Figure Lengend Snippet: Luteoloside activates the p53/p21 pathway in human gastric cancer NCI-N87 cells. (A) Western blot analysis was performed to detect the relative expression levels of p53, p21 and Bcl-2 in NCI-N87 cells in each group; (B) quantified expression levels of p53; (C) quantified expression levels of p21; (D) quantified expression levels of Bcl-2. **P<0.01 vs. the control group.

    Article Snippet: The human GC cell line NCI-N87 (CRL-5822) was purchased from the American Type Culture Collection and incubated in RPMI-1640 medium (cat. no. R8758; Sigma-Aldrich; Merck KGaA) with 10% fetal bovine serum (cat. no. 16000-044; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin at 37°C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Western Blot, Expressing, Control