nci n87 (ATCC)


Structured Review

Nci N87, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nci n87/product/ATCC
Average 97 stars, based on 1 article reviews
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1) Product Images from "Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling"
Article Title: Solamargine inhibits gastric cancer progression via inactivation of STAT3/PD‑L1 signaling
Journal: Molecular Medicine Reports
doi: 10.3892/mmr.2024.13400

Figure Legend Snippet: Solamargine significantly reduces the viability and proliferation of GC cells. GC cells were treated with 5, 10 or 20 µM solamargine for 48 h. The viability of (A) NCI-N87 and (B) HGC-27 cells was assessed using Cell Counting Kit-8 assays. The proliferation of (C) HGC-27 and (D) NCI-N87 cells was investigated using EdU staining. Magnification, ×200. **P<0.01 vs. Control. GC, gastric cancer; EdU, 5-ethynyl-2′-deoxyuridine.
Techniques Used: Cell Counting, Staining, Control

Figure Legend Snippet: Solamargine significantly reduces the migration and invasion of gastric cancer cells. (A) Migration and (B) invasion of NCI-N87 and HGC-27 cells were investigated using Transwell assays. Magnification, ×200. **P<0.01 vs. Control.
Techniques Used: Migration, Control

Figure Legend Snippet: Solamargine reverses the IL-6-induced activation of STAT3/PD-L1 signaling. GC cells were pretreated with 20 ng/ml IL-6 for 24 h, before being co-cultured with Jurkat-T cells for 48 h. Subsequently, GC cells were collected for further analysis. The protein expression levels of c-Myc and PD-L1 in (A) HGC-27 and (B) NCI-N87 cells were assessed using western blotting. Protein levels of p-STAT3 (normalized to STAT3) in (C) HGC-27 and (D) NCI-N87 cells were investigated using immunofluorescence staining. Magnification, ×200. **P<0.01 vs. Control. # P<0.05 and ## P<0.01 vs. IL-6 alone. p-, phosphorylated; PD-L1, programmed cell death ligand 1; GC, gastric cancer.
Techniques Used: Activation Assay, Cell Culture, Expressing, Western Blot, Immunofluorescence, Staining, Control

Figure Legend Snippet: Solamargine activation of T cells. Jurkat T cells were activated using stimulation with anti-CD3/CD28 antibodies and then co-cultured with non-treated or 10 µM solamargine pretreated NCI-N87 or HGC-27 cells. Subsequently, the Jurkat T cells were collected. (A) Representative flow cytometry histograms and (B) the MFI of CD69 in Jurka T cells assessed using flow cytometry. **P<0.01 vs.(−) Jurkat T cell only. ## P<0.01 vs. NCI-N87 control. ^^ P<0.01 vs. HGC-27 control. MFI, mean fluorescence intensity. (−) Jurkat T cell indicates Jurkat T cell without CD3/CD28 stimulation.
Techniques Used: Activation Assay, Cell Culture, Flow Cytometry, Control, Fluorescence