Journal: Frontiers in Microbiology

Article Title: Evaluation of phages and liposomes as combination therapy to counteract Pseudomonas aeruginosa infection in wild-type and CFTR -null models

doi: 10.3389/fmicb.2022.979610

Figure Lengend Snippet: PS/PA liposomes and CKΦ administration does not elicit toxic effects on human CF cells. (A) Cell viability, evaluated by MTT assay, in immortalized human bronchial epithelial cells CuFi-1 expressing F508del CFTR control, treated with phosphatidylserine/phosphatidic acid (PS/PA) liposome, CKΦ or combination of PS/PA liposome-CKΦ for 48 h. (B) Cell viability, evaluated by MTT assay, in THP-1 activated macrophage-like cells treated or not with the CFTR inhibitor. Effects of single or combined PS/PA liposome and CKΦ-administration were evaluated after 48 h of treatment. Unpaired Student’s t test: * p < 0.05; ns, not significant. Results are presented as mean ± SEM.

Article Snippet: The human CF bronchial epithelial cell line CuFi-1 (ATCC CRL-4013™) were grown as monolayer cultures as previously described ( ).

Techniques: MTT Assay, Expressing

Journal: Pharmaceutics

Article Title: Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

doi: 10.3390/pharmaceutics13111787

Figure Lengend Snippet: Transfection capacity and cell viability assays. ( A ) Fluorescence intensity over time in CuFi-1 cells transfected with nioplexes based on formulation 1 , 2 and 3 at cationic lipid/DNA mass ratio 5/1. Each value represents the mean ± SD of three measurements. ( B ) Fluorescence microscope images showing EGFP positive CuFi-1 cells transfected with nioplexes based on formulation 1 , 2 and 3 at cationic lipid/DNA mass ratio 5/1 and cellular appearance over time. ( C ) Percentages of EGFP positive live cells (bars) and cell viability (symbols) measured by flow cytometry in CuFi-1 cells 48 h after transfection with nioplexes 1 and 2 at cationic lipid/DNA mass ratios 2/1, 5/1 and 10/1. Each value represents the mean ± SD of three measurements. ( D ) Mean fluorescence intensity values. Each value represents the mean ± SD of three measurements. Statistical significance: * p ≤ 0.05 in transfection and MFI; # p ≤ 0.05 in cell viability.

Article Snippet: Human cystic fibrosis airway epithelial (CuFi-1) cells obtained from ATCC ® CRL-4013™ were incubated at 37 °C and 5% CO 2 atmosphere in collagen type IV (Merck KGaA, Darmstadt, Germany) pre-treated flasks/plates and were split every 2–3 days to maintain monolayer coverage.

Techniques: Transfection, Fluorescence, Microscopy, Flow Cytometry