Journal: International Journal of Molecular Sciences

Article Title: Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection

doi: 10.3390/ijms23147552

Figure Lengend Snippet: nfGNPs inhibit HPV16 PsV pseudo-infection. ( A ) Fluorescence microscopy of 293TT cells infected with PsV treated with nfGNPs. HPV16 PsV was incubated with increased concentrations of nfGNPs before 293TT cell infection. ( B ) Inhibition of PsV pseudo-infection by nfGNPs. Pseudo-infected 293TT cell fluorescence was quantified by flow cytometry and plotted relative to the non-treated PsV control. The graphs represent the mean and standard deviation of three independent experiments.

Article Snippet: The human embryonic kidney 293TT (ATCC CRL-3467) cells were cultured in Gibco ® Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 5% Gibco ® fetal bovine serum (FBS) (Thermo Fisher Scientific), 100 IU/mL penicillin, 100 µg/mL streptomycin (PAA Laboratories Inc., Pasching, Austria) and 200 μg/mL Gibco ® hygromycin (Thermo Fisher Scientific) at 37 °C and 5% CO 2 .

Techniques: Infection, Fluorescence, Microscopy, Incubation, Inhibition, Flow Cytometry, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection

doi: 10.3390/ijms23147552

Figure Lengend Snippet: nfGNPs inhibit HPV16 PsV pseudo-infection better than heparin. Inhibition of PsV pseudo-infection by nfGNPs, nfSNPs and heparin. Pseudo-infected 293TT cell fluorescence was quantified by flow cytometry and plotted relative to the non-treated PsV control. The graphs represent the mean and standard deviation of three independent experiments.

Article Snippet: The human embryonic kidney 293TT (ATCC CRL-3467) cells were cultured in Gibco ® Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 5% Gibco ® fetal bovine serum (FBS) (Thermo Fisher Scientific), 100 IU/mL penicillin, 100 µg/mL streptomycin (PAA Laboratories Inc., Pasching, Austria) and 200 μg/mL Gibco ® hygromycin (Thermo Fisher Scientific) at 37 °C and 5% CO 2 .

Techniques: Infection, Inhibition, Fluorescence, Flow Cytometry, Standard Deviation

Journal: Cell Reports

Article Title: In vivo characterization of emerging SARS-CoV-2 variant infectivity and human antibody escape potential

doi: 10.1016/j.celrep.2021.109838

Figure Lengend Snippet:

Article Snippet: 293 TT cells , ATCC , CRL-3467.

Techniques: Luciferase, Recombinant, Expressing, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Sequencing, Mutagenesis, Software

Journal: eLife

Article Title: Discovery of several thousand highly diverse circular DNA viruses

doi: 10.7554/eLife.51971

Figure Lengend Snippet:

Article Snippet: Cell line ( Homo-sapiens ) , 293TT cells , https://dtp.cancer.gov/repositories/ , NCI-293TT , Deposition to ATCC in progress.

Techniques: Recombinant, Expressing, Generated, Amplification, Software

Journal: Journal of Virology

Article Title: Human Papillomavirus Replication Regulation by Acetylation of a Conserved Lysine in the E2 Protein

doi: 10.1128/JVI.01912-17

Figure Lengend Snippet: K111 is necessary for transient viral replication. Mutations of E2 lysine 111 (K111) alter transient replication. (A) Sequence alignment shows the highly conserved K111. (B) C33A cells were cotransfected with BPV1 E2-K111 mutants, pFLORI-BPV1, and pRRL and (C) C33A cells were cotransfected with HPV31 E2-K111 mutants. E2 mutants were cotransfected with pFLOri31. The luminescence was normalized to Renilla luciferase and the respective background E2 luminescence. Each of the samples was normalized to its respective control without E1 to cancel out any background effect. (Inset) The effect of K111R mutation on cell viability and cell cycle was determined by flow cytometry. (D) 293TT cells were cotransfected with HPV31 FLAG-E2 and HA-E1. E1 protein pulldown was conducted using 12CA5 antibody and blotted with HA7 and M2 antibodies. *, P < 0.05; **, P < 0.005.

Article Snippet: C33A and HeLa (ATCC), HEK 293TT (J. Schiller, C. Buck), and J23T3 (H. Green) cells were maintained in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Atlas Biologicals) and 100 U/ml penicillin-streptomycin (Life Technologies).

Techniques: Sequencing, Luciferase, Mutagenesis, Flow Cytometry

Journal: Journal of Virology

Article Title: Human Papillomavirus Replication Regulation by Acetylation of a Conserved Lysine in the E2 Protein

doi: 10.1128/JVI.01912-17

Figure Lengend Snippet: HPV31 E2-K111 mutants maintain interactions with cellular factors. (A) 293TT cells were cotransfected with HPV31 FLAG-E2, and plasmids expressing BRD4 fragments CTM, BID, and PDID. BRD4 proteins were pulled down using glutathione S-transferase (GST) antibody and blotted with GST and M2 antibodies. (B) 293TT cells were cotransfected with HPV31 FLAG-E2 and HA-GPS2. HPV31 E2 protein pulldown was executed with M2 antibody and blotted with HA7 and M2 antibodies. Relative GPS2 binding was calculated by densitometry and presented graphically. (C) 293TT cells were transfected with HPV31 FLAG-E2 followed by E2 protein immunoprecipitation with M2 antibody. The amounts of endogenous TopBP1 coimmunoprecipitated were determined by blotting with anti-TopBP1 and anti-M2 antibodies, and relative binding was calculated by densitometry and represented graphically.

Article Snippet: C33A and HeLa (ATCC), HEK 293TT (J. Schiller, C. Buck), and J23T3 (H. Green) cells were maintained in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Atlas Biologicals) and 100 U/ml penicillin-streptomycin (Life Technologies).

Techniques: Expressing, Binding Assay, Transfection, Immunoprecipitation