thle 2  (ATCC)


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    ATCC thle 2
    Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle 2 cells atcc crl 2706 human  (ATCC)


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    ATCC thle 2 cells atcc crl 2706 human
    Thle 2 Cells Atcc Crl 2706 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle2 atcc crl 2706 human  (ATCC)


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    ATCC thle2 atcc crl 2706 human
    Thle2 Atcc Crl 2706 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle 2  (ATCC)


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    ATCC thle 2
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    thle  (ATCC)


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    ATCC thle
    Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , <t>THLE-2</t> (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.
    Thle, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models"

    Article Title: Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/scrt465

    Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , THLE-2 (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.
    Figure Legend Snippet: Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , THLE-2 (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.

    Techniques Used: Concentration Assay

    thle 2  (ATCC)


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    ATCC thle 2
    A) Graphs displaying dose response curves of normalized (GAPDH) Gli1 mRNA expression in indicated tumor cell lines after 48 hour treatment of MS-0022, cyclopamine and GDC-0449. Graphs include standard deviation (n = 3). B) Graphs displaying 4 day dose response curves of cell growth as a % growth of DMSO control (MTS) for PANC-1, SUIT-2, PC-3, FEMX and <t>THLE-2</t> cells treated with MS-0022, GDC-0449 and cyclopamine at the indicated dose, with SD (n = 8) C-G) Soft agar colony formation assay of PANC-1 (21 days) and SUIT-2 (14 days) cells treated with DMSO or MS-0022 and GDC-0449 at the indicated doses (n = 3). Each well was imaged and colonies were counted by eye according to size. Graphs displaying the colony count of larger colonies (0.5–1 mm) are shown in C) for PANC-1 and D) for SUIT-2, with SD. Graphs displaying the colony count of medium (0.2–0.5 mm) and small (<0.2 mm) colonies are shown in E) for PANC-1 and F) for SUIT-2, with SD. H) Passage growth assays with PANC-1, SUIT-2, PC-3 and THLE-2 cell lines treated with DMSO control or 5 µM MS-0022 for 2–3 passages of 5–7 days, displayed as % growth of DMSO control, with SD (n = 3).
    Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Novel Synthetic Smoothened Antagonist Transiently Inhibits Pancreatic Adenocarcinoma Xenografts in a Mouse Model"

    Article Title: A Novel Synthetic Smoothened Antagonist Transiently Inhibits Pancreatic Adenocarcinoma Xenografts in a Mouse Model

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0019904

    A) Graphs displaying dose response curves of normalized (GAPDH) Gli1 mRNA expression in indicated tumor cell lines after 48 hour treatment of MS-0022, cyclopamine and GDC-0449. Graphs include standard deviation (n = 3). B) Graphs displaying 4 day dose response curves of cell growth as a % growth of DMSO control (MTS) for PANC-1, SUIT-2, PC-3, FEMX and THLE-2 cells treated with MS-0022, GDC-0449 and cyclopamine at the indicated dose, with SD (n = 8) C-G) Soft agar colony formation assay of PANC-1 (21 days) and SUIT-2 (14 days) cells treated with DMSO or MS-0022 and GDC-0449 at the indicated doses (n = 3). Each well was imaged and colonies were counted by eye according to size. Graphs displaying the colony count of larger colonies (0.5–1 mm) are shown in C) for PANC-1 and D) for SUIT-2, with SD. Graphs displaying the colony count of medium (0.2–0.5 mm) and small (<0.2 mm) colonies are shown in E) for PANC-1 and F) for SUIT-2, with SD. H) Passage growth assays with PANC-1, SUIT-2, PC-3 and THLE-2 cell lines treated with DMSO control or 5 µM MS-0022 for 2–3 passages of 5–7 days, displayed as % growth of DMSO control, with SD (n = 3).
    Figure Legend Snippet: A) Graphs displaying dose response curves of normalized (GAPDH) Gli1 mRNA expression in indicated tumor cell lines after 48 hour treatment of MS-0022, cyclopamine and GDC-0449. Graphs include standard deviation (n = 3). B) Graphs displaying 4 day dose response curves of cell growth as a % growth of DMSO control (MTS) for PANC-1, SUIT-2, PC-3, FEMX and THLE-2 cells treated with MS-0022, GDC-0449 and cyclopamine at the indicated dose, with SD (n = 8) C-G) Soft agar colony formation assay of PANC-1 (21 days) and SUIT-2 (14 days) cells treated with DMSO or MS-0022 and GDC-0449 at the indicated doses (n = 3). Each well was imaged and colonies were counted by eye according to size. Graphs displaying the colony count of larger colonies (0.5–1 mm) are shown in C) for PANC-1 and D) for SUIT-2, with SD. Graphs displaying the colony count of medium (0.2–0.5 mm) and small (<0.2 mm) colonies are shown in E) for PANC-1 and F) for SUIT-2, with SD. H) Passage growth assays with PANC-1, SUIT-2, PC-3 and THLE-2 cell lines treated with DMSO control or 5 µM MS-0022 for 2–3 passages of 5–7 days, displayed as % growth of DMSO control, with SD (n = 3).

    Techniques Used: Expressing, Standard Deviation, Soft Agar Assay

    thle 2  (ATCC)


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    ATCC thle 2
    Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal liver cell line thle  (ATCC)


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    ATCC normal liver cell line thle
    Normal Liver Cell Line Thle, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle 2 cell  (ATCC)


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    ATCC thle 2 cell
    The RNA binding profiles of EZH2 and JARID2 in HepG2 and <t>THLE-2</t> cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    Thle 2 Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development"

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2022.904633

    The RNA binding profiles of EZH2 and JARID2 in HepG2 and THLE-2 cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    Figure Legend Snippet: The RNA binding profiles of EZH2 and JARID2 in HepG2 and THLE-2 cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Techniques Used: RNA Binding Assay, Expressing

    The expression pattern and functional analysis of EZH2 bound transcripts in HepG2 cells. (A) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (B) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (C) Violin plot showing the difference value between tumor and normal samples for specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (D – F) The three figures showing the similar results presented in (A–C) but for the bound genes by JARID2. (G) Bubble plot showing the top ten enriched biological processes for genes upregulated in HCC compared with adjacent normal samples. (H) Venn diagram showing the overlapped genes between EZH2 binding and DEGs from the TCGA database. (I) GSEA analysis results showing the enriched gene set for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (J) GSEA analysis results showing the enriched gene set for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    Figure Legend Snippet: The expression pattern and functional analysis of EZH2 bound transcripts in HepG2 cells. (A) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (B) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (C) Violin plot showing the difference value between tumor and normal samples for specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (D – F) The three figures showing the similar results presented in (A–C) but for the bound genes by JARID2. (G) Bubble plot showing the top ten enriched biological processes for genes upregulated in HCC compared with adjacent normal samples. (H) Venn diagram showing the overlapped genes between EZH2 binding and DEGs from the TCGA database. (I) GSEA analysis results showing the enriched gene set for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (J) GSEA analysis results showing the enriched gene set for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Techniques Used: Expressing, Functional Assay, Binding Assay

    Analysis of lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (A) Boxplot showing the expression pattern of two lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. The expression levels of lncRNAs were obtained from the TCGA database. (B) Line plot showing the expression pattern of lncRNA H19 bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from the TCGA database. (C) Line plot showing the expression pattern of lncRNA PDXDC2P bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from tumor-normal paired HCC samples in the TCGA database. (D) CLIP-seq reads density presentation for lncRNA H19 in the four CLIP-seq datasets. (E) Bubble plot showing the top ten KEGG pathways of the genes co-expressed with H19.
    Figure Legend Snippet: Analysis of lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (A) Boxplot showing the expression pattern of two lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. The expression levels of lncRNAs were obtained from the TCGA database. (B) Line plot showing the expression pattern of lncRNA H19 bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from the TCGA database. (C) Line plot showing the expression pattern of lncRNA PDXDC2P bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from tumor-normal paired HCC samples in the TCGA database. (D) CLIP-seq reads density presentation for lncRNA H19 in the four CLIP-seq datasets. (E) Bubble plot showing the top ten KEGG pathways of the genes co-expressed with H19.

    Techniques Used: Expressing

    ChIP-seq results showing that EZH2 could increase the expression level of bound genes. (A) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were highly correlated in THLE-2 cells. (B) Heatmap presentation of ChIP-seq samples reads density around the center of peaks from THLE-2 EZH2 and H3K27me3 samples. (C) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were lowly correlated in HepG2 cells. (D) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (E) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells from ChIP-seq results, as well as all the expressed genes. (F) GSEA analysis result showing the enriched gene set for top ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells. (G) GSEA analysis results showing the enriched gene set for bottom-ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    Figure Legend Snippet: ChIP-seq results showing that EZH2 could increase the expression level of bound genes. (A) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were highly correlated in THLE-2 cells. (B) Heatmap presentation of ChIP-seq samples reads density around the center of peaks from THLE-2 EZH2 and H3K27me3 samples. (C) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were lowly correlated in HepG2 cells. (D) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (E) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells from ChIP-seq results, as well as all the expressed genes. (F) GSEA analysis result showing the enriched gene set for top ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells. (G) GSEA analysis results showing the enriched gene set for bottom-ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Techniques Used: ChIP-sequencing, Expressing, Binding Assay

    The interaction between bound RNAs and DNAs by EZH2 and JARID2. (A) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 DNA binding profile (ChIP-seq). (B) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 RNA binding profile (CLIP-seq). (C) Venn diagram showing the bound genes by EZH2 ChIP-seq and CLIP-seq samples from HepG2 cells. (D) Working model of EZH2 and JARID2 in HCC. In HepG2 cells, EZH2 exhibits weak DNA binding, thus activating more onco-expression and then binding more to highly expressed RNAs together with JARID2 to produce a more mature onco-transcript. In THLE-2 cells, the DNA binding ability of EZH2 is maintained and represses the expression of oncogenes, and then reduced binding with RNA to produce a less mature onco-transcript.
    Figure Legend Snippet: The interaction between bound RNAs and DNAs by EZH2 and JARID2. (A) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 DNA binding profile (ChIP-seq). (B) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 RNA binding profile (CLIP-seq). (C) Venn diagram showing the bound genes by EZH2 ChIP-seq and CLIP-seq samples from HepG2 cells. (D) Working model of EZH2 and JARID2 in HCC. In HepG2 cells, EZH2 exhibits weak DNA binding, thus activating more onco-expression and then binding more to highly expressed RNAs together with JARID2 to produce a more mature onco-transcript. In THLE-2 cells, the DNA binding ability of EZH2 is maintained and represses the expression of oncogenes, and then reduced binding with RNA to produce a less mature onco-transcript.

    Techniques Used: ChIP-sequencing, Binding Assay, RNA Binding Assay, Expressing

    thle  (ATCC)


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    ATCC thle
    Thle, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    s clavuligerus atcc 2706  (ATCC)


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    ATCC s clavuligerus atcc 2706
    S Clavuligerus Atcc 2706, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC thle 2
    Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC thle 2 cells atcc crl 2706 human
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    ATCC thle2 atcc crl 2706 human
    Thle2 Atcc Crl 2706 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle  (ATCC)
    99
    ATCC thle
    Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , <t>THLE-2</t> (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.
    Thle, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal liver cell line thle
    Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , <t>THLE-2</t> (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.
    Normal Liver Cell Line Thle, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC thle 2 cell
    The RNA binding profiles of EZH2 and JARID2 in HepG2 and <t>THLE-2</t> cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    Thle 2 Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC s clavuligerus atcc 2706
    The RNA binding profiles of EZH2 and JARID2 in HepG2 and <t>THLE-2</t> cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.
    S Clavuligerus Atcc 2706, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , THLE-2 (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.

    Journal: Stem Cell Research & Therapy

    Article Title: Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models

    doi: 10.1186/scrt465

    Figure Lengend Snippet: Effect of exosomes in cell viability after APAP- and H 2 O 2 -induced injury. Experiments were performed in TAMH (A-C) , THLE-2 (D-F) , and Huh-7 (G-I) hepatocytes. (A, D, G) Cytotoxicity of exosomes at different concentrations in respective cell lines. 0.05 μg/ml and 0.1 μg/ml of exosomes were added to respective concentration of APAP (B, E, H) and H 2 O 2 (C, F, I) , and MTT were performed 24 or 72 hours later. Cell viability was normalized against vehicle control group and expressed in percentage ( n = 6 per group; * P < 0.05 versus APAP or H 2 O 2 non-exosomes-treatment group.

    Article Snippet: THLE-2 obtained from ATCC (Manassas, VA, USA), a human normal liver cell, was cultured in LHC-9 medium supplemented with 10% FBS (Hyclone).

    Techniques: Concentration Assay

    The RNA binding profiles of EZH2 and JARID2 in HepG2 and THLE-2 cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Journal: Frontiers in Oncology

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    doi: 10.3389/fonc.2022.904633

    Figure Lengend Snippet: The RNA binding profiles of EZH2 and JARID2 in HepG2 and THLE-2 cell lines, respectively. (A) Box plot showing the transcriptional level of EZH2 and JARID2 in HCC tumor and adjacent normal samples from TCGA database. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. (B) Overall survival in Liver Hepatocellular Carcinoma patients with different expression levels of EZH2 and JARID2. (C) Heat map presentation of all eight samples reads density around the center of peaks from HepG2 JARID2 sample. (D) Venn diagram showing the overlapped genes among the bound genes by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (E) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (F) Bubble plot showing the top ten enriched KEGG pathways for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Article Snippet: THLE-2 Cell (CRL-2706,ATCC, US) was cultured with the BEGM Bullet Kit (CC-3170, Lonza, USA).

    Techniques: RNA Binding Assay, Expressing

    The expression pattern and functional analysis of EZH2 bound transcripts in HepG2 cells. (A) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (B) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (C) Violin plot showing the difference value between tumor and normal samples for specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (D – F) The three figures showing the similar results presented in (A–C) but for the bound genes by JARID2. (G) Bubble plot showing the top ten enriched biological processes for genes upregulated in HCC compared with adjacent normal samples. (H) Venn diagram showing the overlapped genes between EZH2 binding and DEGs from the TCGA database. (I) GSEA analysis results showing the enriched gene set for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (J) GSEA analysis results showing the enriched gene set for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Journal: Frontiers in Oncology

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    doi: 10.3389/fonc.2022.904633

    Figure Lengend Snippet: The expression pattern and functional analysis of EZH2 bound transcripts in HepG2 cells. (A) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (B) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (C) Violin plot showing the difference value between tumor and normal samples for specifically EZH2-bound genes in HepG2 or THLE-2 cells, as well as all the expressed genes. (D – F) The three figures showing the similar results presented in (A–C) but for the bound genes by JARID2. (G) Bubble plot showing the top ten enriched biological processes for genes upregulated in HCC compared with adjacent normal samples. (H) Venn diagram showing the overlapped genes between EZH2 binding and DEGs from the TCGA database. (I) GSEA analysis results showing the enriched gene set for genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. (J) GSEA analysis results showing the enriched gene set for genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Article Snippet: THLE-2 Cell (CRL-2706,ATCC, US) was cultured with the BEGM Bullet Kit (CC-3170, Lonza, USA).

    Techniques: Expressing, Functional Assay, Binding Assay

    Analysis of lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (A) Boxplot showing the expression pattern of two lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. The expression levels of lncRNAs were obtained from the TCGA database. (B) Line plot showing the expression pattern of lncRNA H19 bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from the TCGA database. (C) Line plot showing the expression pattern of lncRNA PDXDC2P bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from tumor-normal paired HCC samples in the TCGA database. (D) CLIP-seq reads density presentation for lncRNA H19 in the four CLIP-seq datasets. (E) Bubble plot showing the top ten KEGG pathways of the genes co-expressed with H19.

    Journal: Frontiers in Oncology

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    doi: 10.3389/fonc.2022.904633

    Figure Lengend Snippet: Analysis of lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. (A) Boxplot showing the expression pattern of two lncRNAs bound by EZH2 and JARID2 in HepG2 and THLE-2 cell lines. The expression levels of lncRNAs were obtained from the TCGA database. (B) Line plot showing the expression pattern of lncRNA H19 bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from the TCGA database. (C) Line plot showing the expression pattern of lncRNA PDXDC2P bound by EZH2 and JARID2 specific for HepG2 cell line. The expression levels of lncRNAs were obtained from tumor-normal paired HCC samples in the TCGA database. (D) CLIP-seq reads density presentation for lncRNA H19 in the four CLIP-seq datasets. (E) Bubble plot showing the top ten KEGG pathways of the genes co-expressed with H19.

    Article Snippet: THLE-2 Cell (CRL-2706,ATCC, US) was cultured with the BEGM Bullet Kit (CC-3170, Lonza, USA).

    Techniques: Expressing

    ChIP-seq results showing that EZH2 could increase the expression level of bound genes. (A) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were highly correlated in THLE-2 cells. (B) Heatmap presentation of ChIP-seq samples reads density around the center of peaks from THLE-2 EZH2 and H3K27me3 samples. (C) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were lowly correlated in HepG2 cells. (D) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (E) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells from ChIP-seq results, as well as all the expressed genes. (F) GSEA analysis result showing the enriched gene set for top ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells. (G) GSEA analysis results showing the enriched gene set for bottom-ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Journal: Frontiers in Oncology

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    doi: 10.3389/fonc.2022.904633

    Figure Lengend Snippet: ChIP-seq results showing that EZH2 could increase the expression level of bound genes. (A) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were highly correlated in THLE-2 cells. (B) Heatmap presentation of ChIP-seq samples reads density around the center of peaks from THLE-2 EZH2 and H3K27me3 samples. (C) Hierarchical clustering sample correlation showing that EZH2 and H3K27me3 binding profile were lowly correlated in HepG2 cells. (D) Hierarchical clustering heatmap showing the expression pattern of genes specifically bound by EZH2 in HepG2 cells compared with THLE-2 cells. The expression levels of genes were obtained from the TCGA database. The samples with the green bar were adjacent normal samples, and the samples with the red bar were HCC tumor samples. (E) Accumulative plot showing the expression pattern of specifically EZH2-bound genes in HepG2 or THLE-2 cells from ChIP-seq results, as well as all the expressed genes. (F) GSEA analysis result showing the enriched gene set for top ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells. (G) GSEA analysis results showing the enriched gene set for bottom-ranked genes specifically bound by JARID2 in HepG2 cells compared with THLE-2 cells.

    Article Snippet: THLE-2 Cell (CRL-2706,ATCC, US) was cultured with the BEGM Bullet Kit (CC-3170, Lonza, USA).

    Techniques: ChIP-sequencing, Expressing, Binding Assay

    The interaction between bound RNAs and DNAs by EZH2 and JARID2. (A) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 DNA binding profile (ChIP-seq). (B) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 RNA binding profile (CLIP-seq). (C) Venn diagram showing the bound genes by EZH2 ChIP-seq and CLIP-seq samples from HepG2 cells. (D) Working model of EZH2 and JARID2 in HCC. In HepG2 cells, EZH2 exhibits weak DNA binding, thus activating more onco-expression and then binding more to highly expressed RNAs together with JARID2 to produce a more mature onco-transcript. In THLE-2 cells, the DNA binding ability of EZH2 is maintained and represses the expression of oncogenes, and then reduced binding with RNA to produce a less mature onco-transcript.

    Journal: Frontiers in Oncology

    Article Title: Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development

    doi: 10.3389/fonc.2022.904633

    Figure Lengend Snippet: The interaction between bound RNAs and DNAs by EZH2 and JARID2. (A) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 DNA binding profile (ChIP-seq). (B) Heatmap presentation for the reads density of ChIP-seq and CLIP-seq samples around the center of peaks from THLE-2 EZH2 RNA binding profile (CLIP-seq). (C) Venn diagram showing the bound genes by EZH2 ChIP-seq and CLIP-seq samples from HepG2 cells. (D) Working model of EZH2 and JARID2 in HCC. In HepG2 cells, EZH2 exhibits weak DNA binding, thus activating more onco-expression and then binding more to highly expressed RNAs together with JARID2 to produce a more mature onco-transcript. In THLE-2 cells, the DNA binding ability of EZH2 is maintained and represses the expression of oncogenes, and then reduced binding with RNA to produce a less mature onco-transcript.

    Article Snippet: THLE-2 Cell (CRL-2706,ATCC, US) was cultured with the BEGM Bullet Kit (CC-3170, Lonza, USA).

    Techniques: ChIP-sequencing, Binding Assay, RNA Binding Assay, Expressing