l wnt 3a  (ATCC)


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    ATCC l wnt 3a
    L Wnt 3a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m musculus atcc crl 2647 b6  (ATCC)


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    ATCC m musculus atcc crl 2647 b6
    M Musculus Atcc Crl 2647 B6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 2647 mouse  (ATCC)


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    ATCC crl 2647 mouse
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    l wnt3a crl 2647 cells  (ATCC)


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    ATCC l wnt3a crl 2647 cells
    L Wnt3a Crl 2647 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l wnt3a crl 2647 cells  (ATCC)


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    ATCC l wnt3a crl 2647 cells
    (A) Schematic diagrams showing the domain structures of TcdB and the 2 short fragments derived from TcdB (TcdB FBD and TcdB mu ) used in this study. GTD, glucosyltransferase domain; CPD, cysteine protease domain; Delivery/RBD, membrane translocation and receptor-binding domain; CROPs, combined repetitive oligopeptides domain. The structural model of TcdB FBD -CRD7 complex shown is modeled based on the crystal structure of TcdB FBD -CRD2 (PDB code: 6C0B) and CRD7 (PDB code:5T44). TcdB FBD , CRD2, and CRD7 are colored pink, green, and blue, respectively. CRD, cysteine-rich domain. (B) TcdB FBD blocked <t>WNT3A-mediated</t> signaling in MDA-MB-231 cells in a dose-dependent manner, whereas TcdB mu showed no inhibition at nanomolar concentrations. Wnt signaling activity was analyzed using the TOPFLASH/TK-Renilla (TK/RL) dual luciferase reporter assay (error bars indicate mean ± SEM, 3 independent experiments). (C) Wnt signaling activity in MDA-MB-231 cells was monitored using TK/RL assays over 5 days after induction by WNT3A conditioned medium with the indicated concentrations of TcdB FBD or TcdB mu . Error bars indicate mean ± SEM, 3 independent experiments. (D) Nude mice were subcutaneously transplanted with TK/RL-transduced MDA-MB-231 cells and then treated at the indicated time point with TcdB FBD or TcdB mu (20 mg/kg of body weight) by intraperitoneal (i.p.) injection. D-Luciferin was injected 5 min before tumor tissues were isolated and the luciferase activity in tumor tissues was then measured ex vivo and quantified (error bars indicate mean ± SEM, n = 4–5 tumors). Numerical values are in .
    L Wnt3a Crl 2647 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Targeted inhibition of Wnt signaling with a Clostridioides difficile toxin B fragment suppresses breast cancer tumor growth"

    Article Title: Targeted inhibition of Wnt signaling with a Clostridioides difficile toxin B fragment suppresses breast cancer tumor growth

    Journal: PLOS Biology

    doi: 10.1371/journal.pbio.3002353

    (A) Schematic diagrams showing the domain structures of TcdB and the 2 short fragments derived from TcdB (TcdB FBD and TcdB mu ) used in this study. GTD, glucosyltransferase domain; CPD, cysteine protease domain; Delivery/RBD, membrane translocation and receptor-binding domain; CROPs, combined repetitive oligopeptides domain. The structural model of TcdB FBD -CRD7 complex shown is modeled based on the crystal structure of TcdB FBD -CRD2 (PDB code: 6C0B) and CRD7 (PDB code:5T44). TcdB FBD , CRD2, and CRD7 are colored pink, green, and blue, respectively. CRD, cysteine-rich domain. (B) TcdB FBD blocked WNT3A-mediated signaling in MDA-MB-231 cells in a dose-dependent manner, whereas TcdB mu showed no inhibition at nanomolar concentrations. Wnt signaling activity was analyzed using the TOPFLASH/TK-Renilla (TK/RL) dual luciferase reporter assay (error bars indicate mean ± SEM, 3 independent experiments). (C) Wnt signaling activity in MDA-MB-231 cells was monitored using TK/RL assays over 5 days after induction by WNT3A conditioned medium with the indicated concentrations of TcdB FBD or TcdB mu . Error bars indicate mean ± SEM, 3 independent experiments. (D) Nude mice were subcutaneously transplanted with TK/RL-transduced MDA-MB-231 cells and then treated at the indicated time point with TcdB FBD or TcdB mu (20 mg/kg of body weight) by intraperitoneal (i.p.) injection. D-Luciferin was injected 5 min before tumor tissues were isolated and the luciferase activity in tumor tissues was then measured ex vivo and quantified (error bars indicate mean ± SEM, n = 4–5 tumors). Numerical values are in .
    Figure Legend Snippet: (A) Schematic diagrams showing the domain structures of TcdB and the 2 short fragments derived from TcdB (TcdB FBD and TcdB mu ) used in this study. GTD, glucosyltransferase domain; CPD, cysteine protease domain; Delivery/RBD, membrane translocation and receptor-binding domain; CROPs, combined repetitive oligopeptides domain. The structural model of TcdB FBD -CRD7 complex shown is modeled based on the crystal structure of TcdB FBD -CRD2 (PDB code: 6C0B) and CRD7 (PDB code:5T44). TcdB FBD , CRD2, and CRD7 are colored pink, green, and blue, respectively. CRD, cysteine-rich domain. (B) TcdB FBD blocked WNT3A-mediated signaling in MDA-MB-231 cells in a dose-dependent manner, whereas TcdB mu showed no inhibition at nanomolar concentrations. Wnt signaling activity was analyzed using the TOPFLASH/TK-Renilla (TK/RL) dual luciferase reporter assay (error bars indicate mean ± SEM, 3 independent experiments). (C) Wnt signaling activity in MDA-MB-231 cells was monitored using TK/RL assays over 5 days after induction by WNT3A conditioned medium with the indicated concentrations of TcdB FBD or TcdB mu . Error bars indicate mean ± SEM, 3 independent experiments. (D) Nude mice were subcutaneously transplanted with TK/RL-transduced MDA-MB-231 cells and then treated at the indicated time point with TcdB FBD or TcdB mu (20 mg/kg of body weight) by intraperitoneal (i.p.) injection. D-Luciferin was injected 5 min before tumor tissues were isolated and the luciferase activity in tumor tissues was then measured ex vivo and quantified (error bars indicate mean ± SEM, n = 4–5 tumors). Numerical values are in .

    Techniques Used: Derivative Assay, Membrane, Translocation Assay, Binding Assay, Inhibition, Activity Assay, Luciferase, Reporter Assay, Injection, Isolation, Ex Vivo

    l wnt 3a atcc crl 2647 mouse  (ATCC)


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    ATCC l wnt 3a atcc crl 2647 mouse
    L Wnt 3a Atcc Crl 2647 Mouse, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 2647  (ATCC)


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    ATCC crl 2647
    Crl 2647, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 2647 authentication hct 8 cell line  (ATCC)


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    ATCC crl 2647 authentication hct 8 cell line
    Crl 2647 Authentication Hct 8 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 2647  (ATCC)


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    ATCC crl 2647
    Crl 2647, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l wnt 3a crl 2647 cell lines  (ATCC)


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    ATCC l wnt 3a crl 2647 cell lines
    L Wnt 3a Crl 2647 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l wnt 3a  (ATCC)


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    ATCC l wnt 3a
    L Wnt 3a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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