crl-2539  (atcc)

Journal: Nature Communications

Article Title: Two-dimensional coordination risedronate-manganese nanobelts as adjuvant for cancer radiotherapy and immunotherapy

doi: 10.1038/s41467-024-53084-w

Figure Lengend Snippet: a Measuring the longitudinal relaxation rates ( r 1 ) of RMn-NBs and commercial Fe 3 O 4 contrast agent ( n = 3 independent samples). b Measuring the transverse relaxation rates ( r 2 ) of RMn-NBs and commercial Fe 3 O 4 contrast agent ( n = 3 independent samples). c T 2 -weighted MRI of RMn-NBs and commercial Fe 3 O 4 contrast agent ( n = 3 independent samples). d Dynamic MRI after intravenous injection of RMn-NBs (10 mM × 0.2 mL) in CT26 tumor. The doses of Mn 2+ and RANa are 5.5 mg kg −1 and 30.5 mg kg −1 , respectively ( n = 3 mice). e Relative MRI signal intensities of tumor and kidney regions in the 4T1 tumor-bearing mice at various time points ( n = 3 mice). The dynamic concentrations of Mn 2+ accumulated within ( f ) tumor and ( g ) kidney in the 4T1 tumor-bearing mice determined by inductively coupled plasma optical emission spectrometer (ICP-OES), respectively ( n = 3 mice). h Pharmacokinetic profiles of free RANa and RMn-NBs in orthotopic 4T1 tumors. The dynamic concentrations of RANa accumulated within ( i ) tumor and ( j ) kidney of 4T1 tumor-bearing mice determined by HPLC, respectively ( n = 3 mice). All data are shown as mean ± SD. Source data are provided as a file.

Article Snippet: The mouse 3T3 (Cat# CRL-1658), CT26 (Cat# CRL-2638), RAW264.7 (Cat# SC-6005), B16F10-OVA (Cat# CRL-6323), and 4T1 (Cat# CRL-2539) cell lines are purchased and authenticated by the American Type Culture Collection (ATCC).

Techniques: Injection

Journal: Nature Communications

Article Title: Two-dimensional coordination risedronate-manganese nanobelts as adjuvant for cancer radiotherapy and immunotherapy

doi: 10.1038/s41467-024-53084-w

Figure Lengend Snippet: a Schematic illustration of in situ vaccination mediated by RMn-NB-sensitized RT. Created in BioRender. Luo, Z. (2024) BioRender.com/n85g329. b Tumor growth curves, ( c ) isolated tumor photographs, and ( d ) tumor weights of BALB/c mice immunized with Saline + RT and RMn-NBs + RT-treated CT26 tumor cells, respectively ( n = 6 mice). e Percentages of T EM in the spleen analyzed by FCM. The spleens in different groups are collected on day 30 ( n = 6 mice). f Tumor growth curves of 4T1 breast tumor-bearing mice treated with Saline, Saline + RT, RMn-NBs + RT, RMn-NBs + RT + αCD8a and RMn-NBs + RT + αPD-L1 ( n = 6 mice). The doses of Mn 2+ and RANa are 5.5 mg kg −1 and 30.5 mg kg −1 , respectively. X-ray irradiation is performed after intravenous injection of RMn-NBs (10 mM × 0.2 mL) for 6 h. Treatments are performed with 3 fractions (total 6 Gy) on days 0, 3, and 6. αCD8a (10 mg kg −1 ) and αPD-L1 (10 mg kg −1 ) are respectively administered via intraperitoneal injection after RT for 6 h, and treatments are performed on days 0, 3, and 6 ( n = 6 mice). g Weights of 4T1 breast tumor after various treatments ( n = 6 mice). h Survival curves of 4T1 breast tumor-bearing mice ( n = 6 mice). i Quantification of metastatic lesions in the lungs ( n = 6 mice). j Average size of each nodule in the lungs ( n = 6 mice). All data are shown as mean ± SD. Statistical analysis is performed using two-tailed Student’s t -test for comparing two groups, and one-way ANOVA analysis of variance for multiple groups. p > 0.05 represent nonsignificance, while p < 0.05 indicate statistical significance. Source data are provided as a file.

Article Snippet: The mouse 3T3 (Cat# CRL-1658), CT26 (Cat# CRL-2638), RAW264.7 (Cat# SC-6005), B16F10-OVA (Cat# CRL-6323), and 4T1 (Cat# CRL-2539) cell lines are purchased and authenticated by the American Type Culture Collection (ATCC).

Techniques: In Situ, Isolation, Saline, Irradiation, Injection, Two Tailed Test

Journal: The EMBO Journal

Article Title: Cancer cell-intrinsic biosynthesis of itaconate promotes tumor immunogenicity

doi: 10.1038/s44318-024-00217-y

Figure Lengend Snippet: Reagents and tools table

Article Snippet: 4T1 , ATCC , CRL-2539.

Techniques: Enzyme-linked Immunosorbent Assay, Luciferase, Recombinant, Sequencing, Software