Journal: Materials

Article Title: A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

doi: 10.3390/ma14226915

Figure Lengend Snippet: Antimicrobial properties of polymers/Ag 2 O nanocomposites.

Article Snippet: Chitosan , 50–500 , B. subtilis ATCC 6538, E. coli MTCC 1303, P. aeruginosa ATCC 6633, S. aureus MTCC 2453 , 5 mg/mL , [ ] .

Techniques:

Journal: ACS Omega

Article Title: Development of Oxadiazole-Sulfonamide-Based Compounds as Potential Antibacterial Agents

doi: 10.1021/acsomega.1c03379

Figure Lengend Snippet: Zone of Inhibition (ZOI) in mm Determined around the Disk of Various Concentrations of Compounds OX7 and OX11

Article Snippet: S. pneumoniae (MTCC 655), E. faecalis (MTCC 439), P. aeruginosa (ATCC 2453), S. typhimurium (MTCC 3224), and E. coli (ATCC 25922) were streaked on nutrient agar plates and kept in an incubator overnight at 37 °C.

Techniques: Inhibition

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Inhibition of the stem cell factor 248 isoform attenuates the development of pulmonary remodeling disease

doi: 10.1152/ajplung.00114.2019

Figure Lengend Snippet: Characterization of anti-SCF248 mAb. A: using ATCC cell lines CRL-2452, CRL-2453, and CRL-2454 that express no SCF, SCF220, or SCF248, respectively, the monoclonal antibody was used for flow cytometry binding assays to demonstrate specificity. B: monoclonal antibody generated to a peptide from exon 6 of SCF248 was subjected to Biacore surface plasmon resonance (SPR) analysis to the specific peptide. The data generated dose response curves that were assessed to have a fast on rate and a slow off rate with a 4.5 nM KD binding. C: image flow cytometry photos of ATCC SCF248-expressing cells incubated for 5 or 60 min with anti-SCF248 or control IgG1 mAb coupled with Phrodo-red pH-sensitive fluorescent dye. D: mean fluorescent intensity (MFI) of ATCC SCF248-expressing cells incubated with control or anti-SCF248 mAb coupled with phrodo-red dye over time to demonstrate internalization. E: IPF patient lung fibroblast cultures incubated for 15 min with control or Anti-SCF248 mAb coupled with phrodo-red dye showing internalization only in the anti-SCF48 mAb-incubated cells. F: naïve Balb/c/J mice were injected intravenously with either control IgG (250 mg/kg), polyclonal anti-SCF (250 mg/kg), or anti-SCF248 monoclonal ab (100 mg/kg) on day 0, 2, 4, and 6. The peripheral blood was assessed for reticulocyte numbers on day 8 as an indication of reduced erythropoiesis as a % of total cells. Data represent the mean ± SE from eight mice/group. ATCC, American Type Culture Collection; IPF, idiopathic pulmonary fibrosis; SCF, stem cell factor.

Article Snippet: A : using ATCC cell lines CRL-2452, CRL-2453, and CRL-2454 that express no SCF, SCF220, or SCF248, respectively, the monoclonal antibody was used for flow cytometry binding assays to demonstrate specificity.

Techniques: Flow Cytometry, Binding Assay, Generated, SPR Assay, Expressing, Incubation, Injection