snu398  (ATCC)


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    ATCC snu398
    Snu398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    snu398 crl 2233  (ATCC)


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    ATCC snu398 crl 2233
    Snu398 Crl 2233, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    snu398 crl 2233  (ATCC)


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    ATCC snu398 crl 2233
    Snu398 Crl 2233, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    snu398  (ATCC)


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    ATCC snu398
    Snu398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    snu 449  (ATCC)


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    ATCC snu 449
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, <t>SNU-449,</t> and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu 449, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect"

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    Journal: Journal of Oncology

    doi: 10.1155/2021/9046798

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Figure Legend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR

    LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).
    Figure Legend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Techniques Used: Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Infection

    snu  (ATCC)


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    ATCC snu
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and <t>SNU-398</t> were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu/product/ATCC
    Average 99 stars, based on 1 article reviews
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    snu - by Bioz Stars, 2024-04
    99/100 stars

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    1) Product Images from "LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect"

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    Journal: Journal of Oncology

    doi: 10.1155/2021/9046798

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Figure Legend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR

    human hcc cell lines snu 387  (ATCC)


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    ATCC human hcc cell lines snu 387
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in <t>SNU-387,</t> SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Human Hcc Cell Lines Snu 387, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hcc cell lines snu 387/product/ATCC
    Average 99 stars, based on 1 article reviews
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    human hcc cell lines snu 387 - by Bioz Stars, 2024-04
    99/100 stars

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    1) Product Images from "LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect"

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    Journal: Journal of Oncology

    doi: 10.1155/2021/9046798

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Figure Legend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR

    snu 423  (ATCC)


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    ATCC snu 423
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, <t>SNU-423,</t> SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu 423, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    snu 423 - by Bioz Stars, 2024-04
    99/100 stars

    Images

    1) Product Images from "LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect"

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    Journal: Journal of Oncology

    doi: 10.1155/2021/9046798

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Figure Legend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR

    LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).
    Figure Legend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Techniques Used: Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Infection

    LINC00665 targeted miR-214-3p. (a) starBase database showed that miR-214-3p expression in HCC tissues was declined. (b) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (c) miR-214-3p expression was measured by qRT-PCR assay. (d) Binding sites of LINC00665 and miR-214-3p. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g) miR-214-3p expression was measured by qRT-PCR assay in sh-LINC00665/lentiv-LINC00665-treated SNU-423 cells ( n = 3, ∗p < 0.05).
    Figure Legend Snippet: LINC00665 targeted miR-214-3p. (a) starBase database showed that miR-214-3p expression in HCC tissues was declined. (b) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (c) miR-214-3p expression was measured by qRT-PCR assay. (d) Binding sites of LINC00665 and miR-214-3p. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g) miR-214-3p expression was measured by qRT-PCR assay in sh-LINC00665/lentiv-LINC00665-treated SNU-423 cells ( n = 3, ∗p < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase

    LINC00665 enhanced HCC viability and aerobic glycolysis via decreasing miR-214-3p expression. (a, b) LINC00665/miR-214-3p effect on cell viability was determined using CCK-8 and colony formation assay. (c–e) LINC00665/miR-214-3p effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).
    Figure Legend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis via decreasing miR-214-3p expression. (a, b) LINC00665/miR-214-3p effect on cell viability was determined using CCK-8 and colony formation assay. (c–e) LINC00665/miR-214-3p effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Techniques Used: Expressing, CCK-8 Assay, Colony Assay

    LINC00665 targeted miR-214-3p/MAPK1. (a) starBase database showed that MAPK1 was overexpressed in HCC tissues. (b, c) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (d) Binding sites of miR-214-3p and MAPK1. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g, h) MAPK1 expression in SNU-423 cells was measured by qRT-PCR assay. (i) The expressions of p-p38 MAPK and MAPK1 were determined by western blotting ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).
    Figure Legend Snippet: LINC00665 targeted miR-214-3p/MAPK1. (a) starBase database showed that MAPK1 was overexpressed in HCC tissues. (b, c) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (d) Binding sites of miR-214-3p and MAPK1. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g, h) MAPK1 expression in SNU-423 cells was measured by qRT-PCR assay. (i) The expressions of p-p38 MAPK and MAPK1 were determined by western blotting ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Techniques Used: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot

    LINC00665 enhanced HCC viability and aerobic glycolysis via upregulation of MAPK1 expression. (a, b) qRT-PCR and western blotting were applied to detect MAPK1 expression ( n = 3, ∗p < 0.05). (c, d) LINC00665/MAPK1 effect on cell viability was determined using CCK-8 and colony formation assay. ((e–g)) LINC00665/MAPK1 effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).
    Figure Legend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis via upregulation of MAPK1 expression. (a, b) qRT-PCR and western blotting were applied to detect MAPK1 expression ( n = 3, ∗p < 0.05). (c, d) LINC00665/MAPK1 effect on cell viability was determined using CCK-8 and colony formation assay. ((e–g)) LINC00665/MAPK1 effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay

    human hcc cell lines snu398  (ATCC)


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    ATCC human hcc cell lines snu398
    Human Hcc Cell Lines Snu398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hcc cell lines snu398/product/ATCC
    Average 99 stars, based on 1 article reviews
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    hcc cell line snu 398  (ATCC)


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    ATCC hcc cell line snu 398
    DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, <t>SNU-398</t> cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.
    Hcc Cell Line Snu 398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcc cell line snu 398/product/ATCC
    Average 99 stars, based on 1 article reviews
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    hcc cell line snu 398 - by Bioz Stars, 2024-04
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    1) Product Images from "lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation"

    Article Title: lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation

    Journal: BioMed Research International

    doi: 10.1155/2020/6575724

    DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, SNU-398 cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.
    Figure Legend Snippet: DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, SNU-398 cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.

    Techniques Used: Methylation, Transfection, Quantitative RT-PCR, Over Expression

    DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression inhibited cell invasion and migration. Transwell assays were performed to analyze the effects of DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression on the cell invasion (a) and migration (b) of SNU-398 cells. Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. ∗ p < 0.05.
    Figure Legend Snippet: DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression inhibited cell invasion and migration. Transwell assays were performed to analyze the effects of DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression on the cell invasion (a) and migration (b) of SNU-398 cells. Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. ∗ p < 0.05.

    Techniques Used: Over Expression, Migration, Transfection

    snu 398  (ATCC)


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    ATCC snu 398
    Snu 398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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  • snu398  (ATCC)
    99
    ATCC snu398
    Snu398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu398/product/ATCC
    Average 99 stars, based on 1 article reviews
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    snu398 - by Bioz Stars, 2024-04
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    86
    ATCC snu398 crl 2233
    Snu398 Crl 2233, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu398 crl 2233/product/ATCC
    Average 86 stars, based on 1 article reviews
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    snu398 crl 2233 - by Bioz Stars, 2024-04
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    99
    ATCC snu 449
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, <t>SNU-449,</t> and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu 449, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu 449/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snu 449 - by Bioz Stars, 2024-04
    99/100 stars
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    snu  (ATCC)
    99
    ATCC snu
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and <t>SNU-398</t> were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snu - by Bioz Stars, 2024-04
    99/100 stars
      Buy from Supplier

    99
    ATCC human hcc cell lines snu 387
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in <t>SNU-387,</t> SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Human Hcc Cell Lines Snu 387, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hcc cell lines snu 387/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human hcc cell lines snu 387 - by Bioz Stars, 2024-04
    99/100 stars
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    99
    ATCC snu 423
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, <t>SNU-423,</t> SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Snu 423, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu 423/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snu 423 - by Bioz Stars, 2024-04
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    99
    ATCC human hcc cell lines snu398
    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, <t>SNU-423,</t> SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).
    Human Hcc Cell Lines Snu398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hcc cell lines snu398/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human hcc cell lines snu398 - by Bioz Stars, 2024-04
    99/100 stars
      Buy from Supplier

    99
    ATCC hcc cell line snu 398
    DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, <t>SNU-398</t> cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.
    Hcc Cell Line Snu 398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcc cell line snu 398/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcc cell line snu 398 - by Bioz Stars, 2024-04
    99/100 stars
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    99
    ATCC snu 398
    DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, <t>SNU-398</t> cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.
    Snu 398, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu 398/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snu 398 - by Bioz Stars, 2024-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR

    LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Infection

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR

    LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 expression was elevated in HCC and associated with poor prognosis. (a) starBase database showed that LINC00665 expression in HCC tissues was elevated. (b) starBase database showed that high expression of LINC00665 predicted poor prognosis. (c) LINC00665 levels in 50 paired HCC tissues and para-carcinoma normal tissues were measured using qRT-PCR assay. (d) Kaplan-Meier curves were applied to analyze LINC00665 value in predicting patients' overall survival in HCC. (e) LINC00665 levels in SNU-387, SNU-423, SNU-449, and SNU-398 were measured by qRT-PCR assay ( ∗ P < 0.05).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR

    LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis. (a) The transfection efficiencies of sh-LINC00665 and lentiv-LINC00665 were detected by qRT-PCR assay. (b–d) LINC00665 effect on cell viability was determined using CCK-8 and colony formation assay. (e–g) LINC00665 role in regulating cell invasion and migration was assessed by Transwell chambers and wound healing assay. (h–j) The ATP levels, lactate section, and glucose consumption were tested in SNU-449 and SNU-423 cells following sh-LINC00665 or lentiv-LINC00665 infection ( n = 3, ∗p < 0.05 versus sh-NC group; # p < 0.05 versus lentiv-NC group).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Infection

    LINC00665 targeted miR-214-3p. (a) starBase database showed that miR-214-3p expression in HCC tissues was declined. (b) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (c) miR-214-3p expression was measured by qRT-PCR assay. (d) Binding sites of LINC00665 and miR-214-3p. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g) miR-214-3p expression was measured by qRT-PCR assay in sh-LINC00665/lentiv-LINC00665-treated SNU-423 cells ( n = 3, ∗p < 0.05).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 targeted miR-214-3p. (a) starBase database showed that miR-214-3p expression in HCC tissues was declined. (b) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (c) miR-214-3p expression was measured by qRT-PCR assay. (d) Binding sites of LINC00665 and miR-214-3p. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g) miR-214-3p expression was measured by qRT-PCR assay in sh-LINC00665/lentiv-LINC00665-treated SNU-423 cells ( n = 3, ∗p < 0.05).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase

    LINC00665 enhanced HCC viability and aerobic glycolysis via decreasing miR-214-3p expression. (a, b) LINC00665/miR-214-3p effect on cell viability was determined using CCK-8 and colony formation assay. (c–e) LINC00665/miR-214-3p effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis via decreasing miR-214-3p expression. (a, b) LINC00665/miR-214-3p effect on cell viability was determined using CCK-8 and colony formation assay. (c–e) LINC00665/miR-214-3p effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, CCK-8 Assay, Colony Assay

    LINC00665 targeted miR-214-3p/MAPK1. (a) starBase database showed that MAPK1 was overexpressed in HCC tissues. (b, c) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (d) Binding sites of miR-214-3p and MAPK1. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g, h) MAPK1 expression in SNU-423 cells was measured by qRT-PCR assay. (i) The expressions of p-p38 MAPK and MAPK1 were determined by western blotting ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 targeted miR-214-3p/MAPK1. (a) starBase database showed that MAPK1 was overexpressed in HCC tissues. (b, c) Pearson analysis of the association between the expression levels of LINC00665 and miR-214-3p in 50 HCC tissues. (d) Binding sites of miR-214-3p and MAPK1. (e, f) Luciferase gene reporter and RIP assay were applied to assess the crosstalk between LINC00665 and miR-214-3p. (g, h) MAPK1 expression in SNU-423 cells was measured by qRT-PCR assay. (i) The expressions of p-p38 MAPK and MAPK1 were determined by western blotting ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot

    LINC00665 enhanced HCC viability and aerobic glycolysis via upregulation of MAPK1 expression. (a, b) qRT-PCR and western blotting were applied to detect MAPK1 expression ( n = 3, ∗p < 0.05). (c, d) LINC00665/MAPK1 effect on cell viability was determined using CCK-8 and colony formation assay. ((e–g)) LINC00665/MAPK1 effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Journal: Journal of Oncology

    Article Title: LINC00665 Targets miR-214-3p/MAPK1 Axis to Accelerate Hepatocellular Carcinoma Growth and Warburg Effect

    doi: 10.1155/2021/9046798

    Figure Lengend Snippet: LINC00665 enhanced HCC viability and aerobic glycolysis via upregulation of MAPK1 expression. (a, b) qRT-PCR and western blotting were applied to detect MAPK1 expression ( n = 3, ∗p < 0.05). (c, d) LINC00665/MAPK1 effect on cell viability was determined using CCK-8 and colony formation assay. ((e–g)) LINC00665/MAPK1 effects on the ATP levels, lactate section, and glucose consumption were tested in SNU-423 ( n = 3, ∗p < 0.05 versus control group; # p < 0.05 versus lentiv-LINC00665 group).

    Article Snippet: The four human HCC cell lines SNU-387, SNU-423, SNU-449, and SNU-398 were acquired from American Type Culture Collection (ATCC, VA, USA) and kept in RPMI-1640 Medium with 10% fetal bovine serum (FBS) and 1% (v/v) penicillin-streptomycin as supplementations.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay

    DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, SNU-398 cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.

    Journal: BioMed Research International

    Article Title: lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation

    doi: 10.1155/2020/6575724

    Figure Lengend Snippet: DLGAP1-AS2 knockdown upregulated miR-154-5p through methylation. To explore the interaction between DLGAP1-AS2 and miR-154-5p, SNU-398 cells were transfected with DLGAP1-AS2 siRNA or miR-154-5p mimic, followed by the confirmation of transfections by RT-qPCR (a). The effects of DLGAP1-AS2 siRNA silencing on miR-154-5p (b) or the effects of miR-154-5p overexpression on DLGAP1-AS2 (c) were also analyzed by RT-qPCR. MSP was performed to analyze the methylation of miR-154-5p in cells with DLGAP1-AS2 siRNA or NC siRNA transfection (d). Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. M: methylation; U: unmethylation. ∗ p < 0.05.

    Article Snippet: HCC cell line SNU-398 (ATCC, USA) was included.

    Techniques: Methylation, Transfection, Quantitative RT-PCR, Over Expression

    DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression inhibited cell invasion and migration. Transwell assays were performed to analyze the effects of DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression on the cell invasion (a) and migration (b) of SNU-398 cells. Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. ∗ p < 0.05.

    Journal: BioMed Research International

    Article Title: lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation

    doi: 10.1155/2020/6575724

    Figure Lengend Snippet: DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression inhibited cell invasion and migration. Transwell assays were performed to analyze the effects of DLGAP1-AS2 siRNA silencing and miR-154-5p overexpression on the cell invasion (a) and migration (b) of SNU-398 cells. Data of multiple transfection groups were expressed as mean ± SD values of 3 biological replicates. ∗ p < 0.05.

    Article Snippet: HCC cell line SNU-398 (ATCC, USA) was included.

    Techniques: Over Expression, Migration, Transfection