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cemx174 cells (ATCC)

Name: cemx174 cells
Brand: ATCC
Rating: 95 (651 reviews)

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ATCC cemx174 cells
Cemx174 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cemx174 cells/product/ATCC
Average 95 stars, based on 1 article reviews
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cemx174 cells - by Bioz Stars, 2024-10

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Comparison of fermentation products yield for CBP conversion of dilute acid pretreated T1-2, T1-3, and T1-2 wild-type (WT) and transgenic (TG) switchgrass with C. thermocellum , C. bescii , and C. obsidiansis . ( A ) Final total products yield for C. thermocellum . ( B ) Final total products yield for C. thermocellum with hot water extraction of biomass. ( C ) Final total products yield for C. bescii with hot water extraction of biomass. ( D ) Final total products yield for C. obsidiansis with hot water extraction of biomass. The black bar represents yield of total fermentation products acetic acid, lactic acid, and ethanol and the white bar represents total residual sugars; glucose plus cellobiose for C thermocellum ; all biomass sugars for Caldicellulosiruptor sp strains.

Journal: Biotechnology for Biofuels

Article Title: Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach

doi: 10.1186/1754-6834-5-81

Figure Lengend Snippet: Comparison of fermentation products yield for CBP conversion of dilute acid pretreated T1-2, T1-3, and T1-2 wild-type (WT) and transgenic (TG) switchgrass with C. thermocellum , C. bescii , and C. obsidiansis . ( A ) Final total products yield for C. thermocellum . ( B ) Final total products yield for C. thermocellum with hot water extraction of biomass. ( C ) Final total products yield for C. bescii with hot water extraction of biomass. ( D ) Final total products yield for C. obsidiansis with hot water extraction of biomass. The black bar represents yield of total fermentation products acetic acid, lactic acid, and ethanol and the white bar represents total residual sugars; glucose plus cellobiose for C thermocellum ; all biomass sugars for Caldicellulosiruptor sp strains.

Article Snippet: TG: Transgenic; WT: Wild-type; COMT: Caffeic acid 3- O -methyltransferase; COB: C. obsidiansis ; CT: C. thermocellum ; CB: C. bescii ; T1: Generation one; SSF: Simultaneous saccharification and fermentation; CBP: Consolidated bioprocessing; GC-MS: Gas chromatography–mass spectrometry; HW: Hot water pretreatment; DA: Dilute acid pretreatment; m/z: Mass to charge ratio; HPLC: High performance liquid chromatography; ATCC: American Type Culture Collection.

Techniques: Transgenic Assay

Fermentation weight loss over time of C. thermocellum growing on dilute acid pretreated and hot water extracted T1-2, T1-3, and T1-12 wild-type (WT) and transgenic (TG) switchgrass.

Journal: Biotechnology for Biofuels

Article Title: Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach

doi: 10.1186/1754-6834-5-81

Figure Lengend Snippet: Fermentation weight loss over time of C. thermocellum growing on dilute acid pretreated and hot water extracted T1-2, T1-3, and T1-12 wild-type (WT) and transgenic (TG) switchgrass.

Techniques: Transgenic Assay

Comparison of fermentation products yield for CBP conversion of hot water pretreated, hot water extracted T1-3 wild-type (WT) and transgenic (TG) switchgrass with C. thermocellum (A), C. bescii (B), and C. obsidiansis (C). The black bar represents yield of total fermentation products acetic acid, lactic acid, and ethanol and the white bar represents total residual sugars; glucose plus cellobiose for C thermocellum ; all biomass sugars for Caldicellulosiruptor sp strains.

Journal: Biotechnology for Biofuels

Article Title: Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach

doi: 10.1186/1754-6834-5-81

Figure Lengend Snippet: Comparison of fermentation products yield for CBP conversion of hot water pretreated, hot water extracted T1-3 wild-type (WT) and transgenic (TG) switchgrass with C. thermocellum (A), C. bescii (B), and C. obsidiansis (C). The black bar represents yield of total fermentation products acetic acid, lactic acid, and ethanol and the white bar represents total residual sugars; glucose plus cellobiose for C thermocellum ; all biomass sugars for Caldicellulosiruptor sp strains.

Techniques: Transgenic Assay

Ratio of selected lignin constituents with a 2-fold comparable difference and p-value < 0.05 after fermentation of hot water pretreated T1-3 switchgrass by C. bescii or C. obsidiansis versus C. thermocellum (microbe effect); transgenic (TG); wild-type (WT) switchgrass

Journal: Biotechnology for Biofuels

Article Title: Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach

doi: 10.1186/1754-6834-5-81

Figure Lengend Snippet: Ratio of selected lignin constituents with a 2-fold comparable difference and p-value < 0.05 after fermentation of hot water pretreated T1-3 switchgrass by C. bescii or C. obsidiansis versus C. thermocellum (microbe effect); transgenic (TG); wild-type (WT) switchgrass

Techniques: Significance Assay, Transgenic Assay

Ratio of selected lignin constituents with a 2-fold comparable difference and p-value < 0.05 from transgenic (TG) versus wild-type (WT) T1-3 switchgrass fermentation after dilute acid pretreatment for a specified microbe (biomass effect)

Journal: Biotechnology for Biofuels

Article Title: Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach

doi: 10.1186/1754-6834-5-81

Figure Lengend Snippet: Ratio of selected lignin constituents with a 2-fold comparable difference and p-value < 0.05 from transgenic (TG) versus wild-type (WT) T1-3 switchgrass fermentation after dilute acid pretreatment for a specified microbe (biomass effect)

Techniques: Significance Assay, Transgenic Assay

Transduction efficiency and cytotoxicity of SFV and SIN alphaviral vectors in 4 T1 cells. (a) 4 T1 cells were infected with SFV and SIN particles encoding EGFP. At 24 h post-infection, the cells were harvested, stained with PI and subjected to dual FACS analysis. The x-axis and the y-axis represent E GFP and PI fluorescence, respectively. The percentage of living/dead cells and EGFP-positive/negative cells is indicated on the plot. The FACS data shown are from representative experiments (n = 3). The diagram on the left (MTT assay) demonstrates the cytotoxic effects of SFV and SIN infection. An MTT cell viability assay was performed every day for 5 days post-infection. The results are presented as the percentage of viable cells relative to the control (uninfected cells). The error bars indicate the standard error of 3 independent experiments. (b) Repeated infection of 4 T1 cells. The cells were infected with SFV expressing EGFP (pictures show green fluorescence) and then re-infected 24, 48 and 72 h later with SFV expressing DS-Red (pictures show red fluorescence). Fluorometry of DS-Red fluorescence was performed 1 day after each re-infection. The diagrams represent the percentage of fluorescence units in re-infected cells relative to control cells (100%), which were primarily infected with only SFV/DS-Red. The error bars indicate the standard error of three experiments.

Journal: BMC Cancer

Article Title: High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

doi: 10.1186/1471-2407-14-460

Figure Lengend Snippet: Transduction efficiency and cytotoxicity of SFV and SIN alphaviral vectors in 4 T1 cells. (a) 4 T1 cells were infected with SFV and SIN particles encoding EGFP. At 24 h post-infection, the cells were harvested, stained with PI and subjected to dual FACS analysis. The x-axis and the y-axis represent E GFP and PI fluorescence, respectively. The percentage of living/dead cells and EGFP-positive/negative cells is indicated on the plot. The FACS data shown are from representative experiments (n = 3). The diagram on the left (MTT assay) demonstrates the cytotoxic effects of SFV and SIN infection. An MTT cell viability assay was performed every day for 5 days post-infection. The results are presented as the percentage of viable cells relative to the control (uninfected cells). The error bars indicate the standard error of 3 independent experiments. (b) Repeated infection of 4 T1 cells. The cells were infected with SFV expressing EGFP (pictures show green fluorescence) and then re-infected 24, 48 and 72 h later with SFV expressing DS-Red (pictures show red fluorescence). Fluorometry of DS-Red fluorescence was performed 1 day after each re-infection. The diagrams represent the percentage of fluorescence units in re-infected cells relative to control cells (100%), which were primarily infected with only SFV/DS-Red. The error bars indicate the standard error of three experiments.

Article Snippet: BHK-21 (baby hamster kidney cells) and 4 T1 cells (metastasizing mammary carcinoma from BALB/c mice) were obtained from the American Type Culture Collection (ATCC/LGC Prochem, Boras, Sweden).

Techniques: Transduction, Infection, Staining, Fluorescence, MTT Assay, Viability Assay, Expressing

Evaluation of 4 T1 cell proliferation after 5-FU treatment and in combination with SFV infection. (a) 5-FU treatment. 4 T1 cells were grown in cell culture medium (24-well plates) containing the indicated concentrations of 5-FU. The MTT cell viability assay was performed every day for 5 days. The diagram shows the cytotoxic effect of 5-FU on 4 T1 cells as the percentage of viable cells relative to the control (untreated cells). (b) Schematic representation of the combined treatment with SFV and 5-FU. The cells were infected with SFV/EGFP particles, and the medium was replaced 2 days later with medium containing 5-FU. The MTT cell viability assay was performed every day for 5 days. The arrows designate the day of infection (SFV) and the beginning of the drug treatment (5-FU). The diagram shows the cytotoxic effect of 5-FU following SFV infection as the percentage of viable cells relative to the control (untreated cells). The error bars indicate the standard error of 3 independent experiments. The microscopy image shows a 4 T1 cell monolayer at day 5 after treatment with SFV and the highest concentration of 5-FU.

Journal: BMC Cancer

Article Title: High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

doi: 10.1186/1471-2407-14-460

Figure Lengend Snippet: Evaluation of 4 T1 cell proliferation after 5-FU treatment and in combination with SFV infection. (a) 5-FU treatment. 4 T1 cells were grown in cell culture medium (24-well plates) containing the indicated concentrations of 5-FU. The MTT cell viability assay was performed every day for 5 days. The diagram shows the cytotoxic effect of 5-FU on 4 T1 cells as the percentage of viable cells relative to the control (untreated cells). (b) Schematic representation of the combined treatment with SFV and 5-FU. The cells were infected with SFV/EGFP particles, and the medium was replaced 2 days later with medium containing 5-FU. The MTT cell viability assay was performed every day for 5 days. The arrows designate the day of infection (SFV) and the beginning of the drug treatment (5-FU). The diagram shows the cytotoxic effect of 5-FU following SFV infection as the percentage of viable cells relative to the control (untreated cells). The error bars indicate the standard error of 3 independent experiments. The microscopy image shows a 4 T1 cell monolayer at day 5 after treatment with SFV and the highest concentration of 5-FU.

Techniques: Infection, Cell Culture, Viability Assay, Microscopy, Concentration Assay

Inhibition of S FV/DS-Red infection in 4 T1 cells pretreated with 5-FU. 4 T1 cells were treated with high (130 μg ml −1 ) or low (13 μg ml −1 ) concentrations of 5-FU for 2 days, then infected with SFV/DS-Red particles. (a) Fluorescence and phase contrast microscopy pictures. (b) Fluorometric measurement of DS-Red fluorescence in infected cells at 24 h post-infection. The diagram shows the percentage of fluorescence units measured in the cells pretreated with 5-FU (13 μg ml −1 or 130 μg ml −1 ) and then infected with SFV/DS-Red relative to 4 T1 control cells (100%) that were only infected with SFV/DS-Red. The error bars indicate the standard error of three independent experiments.

Journal: BMC Cancer

Article Title: High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

doi: 10.1186/1471-2407-14-460

Figure Lengend Snippet: Inhibition of S FV/DS-Red infection in 4 T1 cells pretreated with 5-FU. 4 T1 cells were treated with high (130 μg ml −1 ) or low (13 μg ml −1 ) concentrations of 5-FU for 2 days, then infected with SFV/DS-Red particles. (a) Fluorescence and phase contrast microscopy pictures. (b) Fluorometric measurement of DS-Red fluorescence in infected cells at 24 h post-infection. The diagram shows the percentage of fluorescence units measured in the cells pretreated with 5-FU (13 μg ml −1 or 130 μg ml −1 ) and then infected with SFV/DS-Red relative to 4 T1 control cells (100%) that were only infected with SFV/DS-Red. The error bars indicate the standard error of three independent experiments.

Techniques: Inhibition, Infection, Fluorescence, Microscopy

Evaluation of cytotoxicity in 4 T1 cells treated with 5-FU and then infected with SFV. (a) Schematic representation of the experiment. 4 T1 cells were pretreated with high (130 μg ml −1 ) or low (13 μg ml −1 ) doses of 5-FU for 2 h or 2 days and then infected with SFV/EGFP particles. (b) 4 T1 cells treated with 5-FU for 2 h and infected with SFV/EGFP particles (solid lines). The dotted lines (red) show the controls: cells treated with 5-FU for 2 h and then incubated in complete medium for 5 days. The dashed line (green) shows the cells infected with SFV/EGFP particles. (c) 4 T1 cells treated with 5-FU for 2 days and infected with SFV/EGFP particles (solid lines). The dotted lines (red) show the controls: cells treated with 5-FU for 2 days (day 0–2) and then incubated in complete medium for further three days. An MTT cell viability assay was performed every day for 5 days. The diagrams show the cytotoxic effects of 5-FU and SFV/EGFP, which are expressed as the percentage of viable cells relative to the untreated cells. Arrows indicate the beginning of drug treatment (5-FU) and the day of infection (SFV). Error bars show the standard error of three experiments. Fluorescent images demonstrate the efficiency of SFV/EGFP expression on the day after infection of 5-FU pretreated 4 T1 cells.

Journal: BMC Cancer

Article Title: High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

doi: 10.1186/1471-2407-14-460

Figure Lengend Snippet: Evaluation of cytotoxicity in 4 T1 cells treated with 5-FU and then infected with SFV. (a) Schematic representation of the experiment. 4 T1 cells were pretreated with high (130 μg ml −1 ) or low (13 μg ml −1 ) doses of 5-FU for 2 h or 2 days and then infected with SFV/EGFP particles. (b) 4 T1 cells treated with 5-FU for 2 h and infected with SFV/EGFP particles (solid lines). The dotted lines (red) show the controls: cells treated with 5-FU for 2 h and then incubated in complete medium for 5 days. The dashed line (green) shows the cells infected with SFV/EGFP particles. (c) 4 T1 cells treated with 5-FU for 2 days and infected with SFV/EGFP particles (solid lines). The dotted lines (red) show the controls: cells treated with 5-FU for 2 days (day 0–2) and then incubated in complete medium for further three days. An MTT cell viability assay was performed every day for 5 days. The diagrams show the cytotoxic effects of 5-FU and SFV/EGFP, which are expressed as the percentage of viable cells relative to the untreated cells. Arrows indicate the beginning of drug treatment (5-FU) and the day of infection (SFV). Error bars show the standard error of three experiments. Fluorescent images demonstrate the efficiency of SFV/EGFP expression on the day after infection of 5-FU pretreated 4 T1 cells.

Techniques: Infection, Incubation, Viability Assay, Expressing

SFV expression in 4 T1 tumor-bearing mice treated with 5-FU. (a) Experiment design: Balb/c mice (n = 5 in each group) were subcutaneously inoculated with 4 T1 cells; beginning on day 14, the mice were treated four times with 5-FU, every other day (40 mg kg −1 , 150 mg kg −1 or 400 mg kg −1 ). On day 20, after the last 5-FU administration, the mice were i.t. or i.p. inoculated with SFV/EnhLuc virus particles. Tumor weight and Luc gene expression were measured 24 h after viral inoculation. (b) Intratumoral Luc gene expression after i.t injection of SFV/EnhLuc virus particles in 5-FU-treated mice. Luciferase activity was measured in tumor homogenates 24 h after virus inoculation. Tumor weights were measured prior to homogenization (scale on the right). (c) SFV/EnhLuc virus biodistribution in 4 T1 tumor-bearing mice treated with 40 mg kg −1 5-FU. Luciferase activity was measured in tumor and organ homogenates 24 h after i.p. virus inoculation. The graphs present the RLUs per mg protein in each organ or tumor (see Methods section). The results are presented as the means ± s.e. The average RLU values and tumor weights are indicated in the tables. RLU, relative light unit.

Journal: BMC Cancer

Article Title: High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model

doi: 10.1186/1471-2407-14-460

Figure Lengend Snippet: SFV expression in 4 T1 tumor-bearing mice treated with 5-FU. (a) Experiment design: Balb/c mice (n = 5 in each group) were subcutaneously inoculated with 4 T1 cells; beginning on day 14, the mice were treated four times with 5-FU, every other day (40 mg kg −1 , 150 mg kg −1 or 400 mg kg −1 ). On day 20, after the last 5-FU administration, the mice were i.t. or i.p. inoculated with SFV/EnhLuc virus particles. Tumor weight and Luc gene expression were measured 24 h after viral inoculation. (b) Intratumoral Luc gene expression after i.t injection of SFV/EnhLuc virus particles in 5-FU-treated mice. Luciferase activity was measured in tumor homogenates 24 h after virus inoculation. Tumor weights were measured prior to homogenization (scale on the right). (c) SFV/EnhLuc virus biodistribution in 4 T1 tumor-bearing mice treated with 40 mg kg −1 5-FU. Luciferase activity was measured in tumor and organ homogenates 24 h after i.p. virus inoculation. The graphs present the RLUs per mg protein in each organ or tumor (see Methods section). The results are presented as the means ± s.e. The average RLU values and tumor weights are indicated in the tables. RLU, relative light unit.

Techniques: Expressing, Injection, Luciferase, Activity Assay, Homogenization

The pH results were obtained according to the methods, treatments and storage time applied to blank processed cheese samples.

Journal: Foods

Article Title: Influence of Emulsifying Salts on the Growth of Bacillus thuringiensis CFBP 3476 and Clostridium perfringens ATCC 13124 in Processed Cheese

doi: 10.3390/foods11203217

Figure Lengend Snippet: The pH results were obtained according to the methods, treatments and storage time applied to blank processed cheese samples.

Article Snippet: Results of the in vitro inhibitory activity, for both treatments T1 (1.5% ESSP) and T2 (1.0% ESSP + 0.5% BSLP), showed that B. thuringiensis CFBP 4376 growth was inhibited, whereas C. perfringens ATCC 13124 was not inhibited.

Techniques:

The Aw results were obtained according to the methods, treatments and storage time applied to blank processed cheese samples.

Journal: Foods

Article Title: Influence of Emulsifying Salts on the Growth of Bacillus thuringiensis CFBP 3476 and Clostridium perfringens ATCC 13124 in Processed Cheese

doi: 10.3390/foods11203217

Figure Lengend Snippet: The Aw results were obtained according to the methods, treatments and storage time applied to blank processed cheese samples.

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