e coli k12 (ATCC)

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ATCC e coli k12
E Coli K12, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/CRL-1663/UMR-108/pm39431850-263-12-21
Average 91 stars, based on 31 article reviews

e coli k12 - by Bioz Stars, 2026-09

91/100 stars

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Polymerase Chain Reaction:

Article Title: The role of Blastocystis hominis in the activation of ulcerative colitis
Article Snippet: .. The B. hominis Brumpt (subtype 1, ATCC®50752 TM ) strain from the American Type Culture Collection was used as the reference strain in the present study. table ft1 table-wrap mode="anchored" t5 caption a7 Primer name Blasto FWD F5 GGTCCGGTGAACACTTTGGATTT 1641–1663 in {"type":"entrez-nucleotide","attrs":{"text":"AY244621","term_id":"37935750","term_text":"AY244621"}} AY244621 Blasto R F2 CCTACGGAAACCTTGTTACGACTTCA 1734–1759 in {"type":"entrez-nucleotide","attrs":{"text":"AY244621","term_id":"37935750","term_text":"AY244621"}} AY244621 Blastocystis probe FAM TCGTGTAAATCTTACCATTTAGAGGA-BHQ1 1705–1730 in AY244321b Open in a separate window Primers used for PCR amplification Statistical analysis Descriptive statistics are presented as frequency (n), percentage (%), median (minimum-maximum), and mean±standard deviation. ..

Amplification:

Article Title: Identification of Two Porcine Reproductive and Respiratory Syndrome Virus Variants Sharing High Genomic Homology but with Distinct Virulence
Article Snippet: .. Primer Sequence (5’-3’) locationa Length of amplicon PRRSV-1F ATGACGTATAGGTGTTGGCTCTATGC 1-26 1641 bp PRRSV-1R AGGGAGCCTGAGGATTTGGAT 1621-1641 PRRSV-2F ATCGCCAACCGGATGGT 1522-1538 1747 bp PRRSV-2R CGATGATGGCTTGAGCTGAGTA 3247-3268 PRRSV-3F TGTCATCAAGCAGCTCCCTGT 3200-3220 1661 bp PRRSV-3R AAGGACGAGGTTCGCGGT 4843-4860 PRRSV-4F TTTCCCGCTGGAGTGAAAGTT 4756-4776 1688 bp PRRSV-4R GCTGTCAGAAGCCTGATCATCAG 6421-6443 PRRSV-5F AATGAGATTCTCCCAGCTGTCCT 6331-6353 1757 bp PRRSV-5R GCGCCTAATATCACAAGCCTGTAT 8064-8087 PRRSV-6F GGAAACACTGGGATTGATGGC 7983-8003 1760 bp PRRSV-6R CCACACCAGATTATAACAGGACAATG 9717-9742 PRRSV-7F CGTACGCCACTGCCTGTG 9661-9678 1663 bp PRRSV-7R GGGAGGGACTCAGCAACTTCT 11303-11323 PRRSV-8F TGCTTCCGGAGACAGTCTTCA 11230-11250 1749 bp PRRSV-8R GAACCATGAACCCTAGTTCGTCAT 12955-12978 PRRSV-9F TGAACTCATGGTGAATTACACGGT 12830-12853 1619 bp PRRSV-9R GTAATGGAAAACGCCAAAAGCA 14427-14448 PRRSV-10F GAGTTGTGCTTGATGGTTCCG 14320-14340 1093 bp PRRSV-10R TAATTACGGCCGCATGGTTC 15393-15412 a The location is determined based on the representative strain ATCC VR-2332 (PRU87392). ..

Sequencing:

Transfection:

Article Title: ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Mus musculus) Epo GenBank NM_007942.2 Erythropoietin Gene (Mus musculus) Arid1a GenBank NM_001080819.2 Arid1a Gene (Mus musculus) Ctnnb1 GenBank NM_007614.3 Beta-catenin Gene (Mus musculus) Apc GenBank NM_001360980.1 Adenomatous polyposis coli Strain, strain background (Mus musculus) Arid1a-lox From Z. Wang’s lab Arid1atm1.1Zhwa/J https://www.jax. org/strain/027717 Strain, strain background (Mus musculus) Apc-lox From PerretColnot’s lab Apctm2.1Cip https://www. infrafrontier. eu/search? keyword= EM:05566 Strain, strain background (Mus musculus) Ttr-Cre-Tam From PerretColnot’s lab Tg(Ttr-cre/ Esr1*)1Vco https://www. infrafrontier. eu/search? keyword= EM:01713 Genetic reagent (Adenovirus 5) Ad-Cre Université de Nantes, France Ad5-CAG-Cre https://umr1089.univnantes.fr/facilitiescores/cpv/translationalvector-core-2201753. kjsp?RH=1519296751975 Cell line (Mus musculus) Mouse hepatoma From Christine Perret’s lab Hepa 1-6 [Hepa16] (ATCC CRL-1830) For transfection experiments Antibody anti-Arid1a (Rabbit monoclonal) Abcam Cat# 182560 [EPR13501] IHC(1:1000), WB (1:2000) Antibody anti-Glul (GS) (Mouse monoclonal) BD Biosciences Cat# 610518, RRID:AB_397880 IHC(1:400), WB (1:5000) Antibody anti-HBB (Mouse monoclonal) Proteintech Cat# 16216–1-AP, RRID:AB_10598329 IHC(1:200), WB (1:2000) Antibody anti-HIF1a (Rabbit polyclonal) Novus Cat# NB100-449, RRID:AB_10001045 WB nuclear extract (1:500) Antibody anti-HIF2a (Rabbit polyclonal) Novus Cat# NB100-122, RRID:AB_10002593 WB nuclear extract (1:500) Antibody Anti-Tcf4 (Tcf7l2) (Mouse monoclonal) Millipore Cat# 05–511, RRID:AB_309772 ChIP: 3 mg Antibody Anti-H3K27Ac (Rabbit polyclonal) Active Motif Cat# 39133, RRID:AB_2561016 ChIP: 3 mg Antibody Anti-H3K27me3 (Rabbit polyclonal) Active Motif Cat# 39155, RRID:AB_2561020 ChIP: 3 mg Continued on next page Riou et al. eLife 2020;9:e53550. .. DOI: https://doi.org/10.7554/eLife.53550 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody IgG (Mouse) Thermo Fisher Scientific Cat# 10400C, RRID:AB_2532980 ChIP: 3 mg Antibody Anti-CD71FITC (Rat monoclonal) BD Biosciences Cat# 553266, RRID:AB_394743 FACS (1:100) Antibody Anti-Ter119PE (rat monoclonal) BD Biosciences Cat# 553673, RRID:AB_394986 FACS (1:100) Antibody Anti-b-actin (mouse monoclonal) Sigma-Aldrich Cat# A5441, RRID:AB_476744 WB (1:10000) Antibody Anti-lamin A/C (rabbit polyclonal) Cell Signaling Technology Cat# 2032, RRID:AB_2136278 WB nuclear extract (1:500) Antibody IgG, HRPconjugated (horse, anti-mouse) Cell Signaling Technology Cat# 7076, RRID:AB_330924 WB (1:2000) Antibody IgG, HRP-conjugated (goat, anti-rabbit) Cell Signaling Technology Cat# 7074, RRID:AB_2099233 WB (1:2000) Antibody IgG, biotinylated (goat, anti-rabbit) Vector lab Cat# BA-1000, RRID:AB_2313606 IHC (1:200) Commercial assay or kit MOM mouse on mouse Vector Laboratories Cat# BMK-2202, RRID:AB_2336833 Kit Sequencebased reagent 18S Thermo Fisher Scientific Taqman Assay 4308329 qPCR primers Sequencebased reagent Glul Thermo Fisher Scientific Taqman Assay Mm00725701_si qPCR primers Mus musculus Sequencebased reagent Axin2 Thermo Fisher Scientific Taqman Assay Mm00443610_m1 qPCR primers Mus musculus Sequencebased reagent Arid1a (total) Thermo Fisher Scientific Taqman Assay Mm00473838_m1 qPCR primers Mus musculus Sequencebased reagent Arid1a (not excised by Cre) Thermo Fisher Scientific Taqman Assay Mm00473841_m1 qPCR primers Mus musculus Sequencebased reagent Apc (total) Thermo Fisher Scientific Taqman Assay Mm00545877_m1 qPCR primers Mus musculus Sequencebased reagent Apc (not excised by Cre) Thermo Fisher Scientific Taqman Assay Mm01130462_m1 qPCR primers Mus musculus Sequencebased reagent Epo Thermo Fisher Scientific Taqman Assay Mm01202755_m1 qPCR primers Mus musculus Sequencebased reagent 18 s Eurogentec F_GTAACCCGT TGAACCCCATT R_CCATCCAA TCGGTAGCG SybrGreen qPCR primers Sequencebased reagent Angiopoietinlike 2 (Angptl2) Eurogentec F_CCGCAACAT GAACTCGAGAG R_GTGCTCCAGG TCCTTGTACT SybrGreen qPCR primers Mus musculus Sequencebased reagent Carbonic anhydrase 9 (Car9) Eurogentec F_GACCTCGTG ATTCTCGGCTA R_GAGAAGGC CAAACACCAAGG SybrGreen qPCR primers Mus musculus Continued on next page Riou et al. eLife 2020;9:e53550.

Immunohistochemistry:

Western Blot:

Chromatin Immunoprecipitation:

Biomarker Discovery:

Article Title: SmJAZ8 acts as a core repressor regulating JA-induced biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots.
Article Snippet: .. D ow nloaded from https://academ ic.oup.com /jxb/article/69/7/1663/4770216 by guest on 22 M ay 2024 In our validation of the different expression patterns of these genes, the expression level of bHLH (SMil_00020000) was suppressed in the J8O1 lines, whereas TAT, STPS, GDPD, and CYP71D10 levels were increased when compared with the ATCC hairy root lines under MeJA treatment, in agreement with the RNA-seq data. .. In the MeJA-induced J8R3 lines, the expression levels of GPT and bHLH (SMil_00010404 and SMil_00020000) were down-regulated, whereas those of TAT, AP2/ERF (SMil_00009117), and NAC were up-regulated compared with ATCC under the MeJA treatment (Supplementary Fig. S8).

Expressing:

RNA Sequencing:

Virus:

Article Title: Rational Engineering of a Synthetic Insect-Bacterial Mutualism
Article Snippet: Rational engineering of a synthetic insect-bacterial mutualism Yinghua Su*, Ho-Chen Lin, Li Szhen Teh, Fabienne Chevance, Ian James, Clara Mayfield, Kent G. Golic, James A. Gagnon, Ofer Rog, Colin Dale1* School of Biological Sciences, University of Utah, Salt Lake City, UT, 84112, United States of America.. 1Lead contact *Correspondence: crystal.su@utah.edu (Y. Su), colin.dale@utah.edu (C. Dale) This preprint research paper has not been peer reviewed.. Electronic copy available at: https://ssrn.com/abstract=4082412 Pr ep rin t n ot p ee r r ev ie we d

Review