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hek293  (ATCC)
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ATCC hek293
A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Hek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek293 cells/product/ATCC
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A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Hek 293 Cells Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Human 227 Embryonic Kidney Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Hek 293 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek 293 cell lines/product/ATCC
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A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and <t>HEK293</t> cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).
Hek 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek 293 cell line/product/ATCC
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hek 293 cell line - by Bioz Stars, 2025-01
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Image Search Results


A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and HEK293 cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).

Journal: bioRxiv

Article Title: Processing of Genomic RNAs by Dicer in Bat Cells Limits SARS-CoV-2 Replication

doi: 10.1101/2025.01.10.632425

Figure Lengend Snippet: A) Differential Expression of mRNAs in TBLU-ACE2 cells after SARS-2-CoV-2 infection. Genes of interest are colored by red dots and designated with arrows and labels. Grey dots in the blot are not significantly different based on P-value ≤ 0.05. B) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and upregulated greater than 2 log2 fold change. C) GO analysis of genes graphed in part A that were assigned a P-value ≤ 0.05 and downregulated less than -2 log2 fold change. D) Expression changes expressed as log2 fold change of select genes based on RNA sequencing after Poly(I:C) treatment in TBLU cells compared to untreated controls. E) Western blot analysis of phospho-PKR and phospho-eIF2α in Poly(I:C)-treated, or SARS-CoV-2 infected TBLU and HEK293 cells. TBLU cells were also transfected with plasmids encoding either PKR or eIF2α as shown by the plus and minus designations below the blot bands. F) LDH cytotoxicity assay measuring cell viability in Poly(I:C)-treated TBLU, HEK293, and PKR or eIF2α transfected bat cells. Statistical significance determined by Tukey ANOVA (P-value ≤ 0.05).

Article Snippet: The lung epithelial bat cell line TBLU (catalogue no. CCL-88; ATCC), HEK293 (catalogue no. CRL-1573; ATCC), and Vero E6 (catalogue no. CRL-1586, ATCC) was cultured in DMEM:F-12 (Gibco) supplemented with 10% FBS and Penicillin streptomycin.

Techniques: Expressing, Infection, RNA Sequencing Assay, Western Blot, Transfection, LDH Cytotoxicity Assay