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Culturing Adherent HEK 293 T Cells in ColTubes. Phase-contrast and Live/Dead staining images of adherent HEK 293 cells (A) and suspension 293 T 17SF cells (B) in ColTubes.

Journal: Biofabrication

Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing

doi: 10.1088/1758-5090/ae2718

Figure Lengend Snippet: Culturing Adherent HEK 293 T Cells in ColTubes. Phase-contrast and Live/Dead staining images of adherent HEK 293 cells (A) and suspension 293 T 17SF cells (B) in ColTubes.

Article Snippet: For a typical cell culture, adherent HEK293 cells (#CRL-1573, ATCC) loaded in 20 μ l ColTubes were suspended in 2 ml DMEM supplemented with 10% fetal bovine serum (FBS) in a 6-well plate and incubated at 37 °C with 5% CO2 and 21% O2.

Techniques: Staining, Suspension