hek293  (ATCC)

Journal: Biofabrication

Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing

doi: 10.1088/1758-5090/ae2718

Figure Lengend Snippet: Culturing Adherent HEK 293 T Cells in ColTubes. Phase-contrast and Live/Dead staining images of adherent HEK 293 cells (A) and suspension 293 T 17SF cells (B) in ColTubes.

Article Snippet: For a typical cell culture, adherent HEK293 cells (#CRL-1573, ATCC) loaded in 20 μ l ColTubes were suspended in 2 ml DMEM supplemented with 10% fetal bovine serum (FBS) in a 6-well plate and incubated at 37 °C with 5% CO2 and 21% O2.

Techniques: Staining, Suspension

Journal: iScience

Article Title: Bta-miR-30f promotes adipogenesis and unsaturated fatty acid accumulation by targeting RAD23B in buffalo intramuscular preadipocytes

doi: 10.1016/j.isci.2025.114578

Figure Lengend Snippet: RAD23B is a target gene of bta-miR-30f (A) Prediction of target genes for bta-miR-30f by TargetScan (cyan) and mirWalk (orange). (B) Predicted duplex formation between RAD23B 3′-UTR and bta-miR-30f using RNAhybrid. (C) The mRNA expression level of RAD23B in bta-miR-30f overexpression or inhibition ( n = 4 biologically independent experiments). (D) Schematic diagram shows the RAD23B wild-type (WT) and mutant (Mut) luciferase reporter vectors for bta-miR-30f. (E) Luciferase activity in HEK293T cells after transfection with WT or mutant RAD23B and bta-miR-30f or MNC for 48 h ( n = 3 biologically independent experiments). All data presented mean ± SE; (C and E) Statistical analyses using unpaired t test were performed on GraphPad Prism 8.0, where ∗ p < 0.05.

Article Snippet: HEK293T cells , ATCC , CRL-1573.

Techniques: Expressing, Over Expression, Inhibition, Mutagenesis, Luciferase, Activity Assay, Transfection