rissen (Worthington Biochemical)

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Papain Suspension

Description:

Supplied as a 2X crystalline suspension in 50 mM sodium acetate pH 4 5 To insure full activity the enzyme should be incubated in a solution containing 1 1 mM EDTA 0 067 mM mercaptoethanol and 5 5 mM cysteine HCl for 30 minutes It is recommended that the enzyme be 0 22 micron filtered after dissolution and prior to use

Catalog Number:

ls003124

Price:

Size:

25 mg

Source:

Papaya Latex

Cas Number:

9001.73.4

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Structured Review

Worthington Biochemical rissen
Supplied as a 2X crystalline suspension in 50 mM sodium acetate pH 4 5 To insure full activity the enzyme should be incubated in a solution containing 1 1 mM EDTA 0 067 mM mercaptoethanol and 5 5 mM cysteine HCl for 30 minutes It is recommended that the enzyme be 0 22 micron filtered after dissolution and prior to use
https://www.bioz.com/result/rissen/product/Worthington Biochemical
Average 90 stars, based on 1287 article reviews
Price from $9.99 to $1999.99

rissen - by Bioz Stars, 2021-01

90/100 stars

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Incubation:

Article Title: Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling
Article Snippet: .. To perform papain digestion, brains were cut into 6 pieces and incubated in 5 mL HBSS containing 50U/mL DNase (Roche), and 4U/mL papain (Worthington-Biochem, Cat#LS003126) for 45 min at 37°C. .. Tissue was triturated first with a large bore glass pipette tip, and twice with a small-bore pipette tip every 15 min.

Article Title: Stratifying nonfunctional pituitary adenomas into two groups distinguished by macrophage subtypes
Article Snippet: .. Tissue was manually minced with a razor blade and incubated in papain dissociative agent (Worthington Biochemical; LS003118) for 30 minutes. ..

Article Title: Complementary networks of cortical somatostatin interneurons enforce layer specific control
Article Snippet: .. Following recovery, tissue was transferred to a solution of 10U/ml Papain (Worthington LK003176), dissolved in HEPES-ACSF (NaCl 120mM, KCl 5mM, MgCl2 2mM, Glucose 25mM, CaCl2 5mM, HEPES 10mM, pH 7.4 and supplemented with 1mMol solution of kynurenic acid sodium salt (Abcam 120256) and previously activated with 2.5 mM Cysteine and 2.5 mM ethylenediaminetetraacetic acid (EDTA) for 20 min at 34 ° C, and incubated for 25 min at 34 ° C under carbogen. .. Digestion was attenuated with 4 ° C Stop Solution (10% ovomuccoid inhibitor (Worthington LK003182, resuspended in EBSS to manufacturer’s specifications) in HEPES-ACSF).

Article Title: Role for Wnt signaling in retinal neuropil development: analysis via RNAseq and in vivo somatic CRISPR mutagenesis
Article Snippet: .. Retinas were washed in Ca- Mg- free HBSS (Gibco) and incubated in papain solution (40 units papain suspension (Worthington), 300 units DNase I (Sigma), 10 mM HEPES in HBSS; 2.5 mL for 4 retinas) for 18 minutes at 37°C. .. Papain solution was removed and inactivated with 1 mL low-ovomucoid (LO) solution (1.5% BSA (Sigma) and 1.5% ovomucoid (Worthington)) in Eagle’s minimal essential medium (MEM).

other:

Article Title: A painful neuropathy-associated Nav1.7 mutant leads to time-dependent degeneration of small-diameter axons associated with intracellular Ca2+ dysregulation and decrease in ATP levels
Article Snippet: DRG were pooled from two animals and after careful removal of meninges were enzymatically digested with Collagenase A ((70474031, Roche) 1.5 mg/ml in CSS containing 0.6 mM ethylene diamine tetraacetic acid) for 15 min at 37℃ with mild agitation, followed by Collagenase D ((70507821, Roche) 1.5 mg/ml in CSS, containing 30 U/ml Papain (LS003126, Worthington Biochemical) and 0.6 mM ethylene diamine tetraacetic acid) for 15 min at 37℃ with mild agitation.

Article Title: Differential immune microenvironments and response to immune checkpoint blockade amongst molecular subtypes of murine medulloblastoma
Article Snippet: Tumors were dissociated to a single cell suspension following mechanical disruption and papain (Worthington) digestion.

Article Title: Phosphorylation of tau at Y18, but not tau-fyn binding, is required for tau to modulate NMDA receptor-dependent excitotoxicity in primary neuronal culture
Article Snippet: Hippocampi of newborn pups (P0–P1) were dissected in ice-cold Earle’s balanced salt solution (EBSS), lacking CaCl2 , MgSO4 and phenol red (Thermo Fisher Scientific, 14155), digested with papain (Worthington LK003176, ~1 unit per hippocampus) in EBSS at 37 °C for 15 min, and triturated in a disposable plastic tube in low ovomucoid solution: Dulbecco’s phosphate-buffered saline (DPBS, Thermo Fisher Scientific, 14040-182) containing 1.5 mg/ml BSA (Sigma-Aldrich, A7030-10G), 1.5 mg/ml trypsin inhibitor (Sigma-Aldrich, T9253-5G), and 66.7 units/ml DNase I (Sigma-Aldrich, D5025).

Injection:

Article Title: Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice
Article Snippet: .. Retinas from injected eyes that fit in the inclusion conditions for control, Mild IOP, and Moderate IOP were dissected and enzymatically dissociated with 9 units/mL of papain (LS003126, Worthington, Lakewood, NJ, USA) for 45 mins at 34 °C. .. Retinal dissociations were then performed by trituration and filtration through a 20 µm nylon mesh (#SCNY00020, EMDMillipore, Burlington, MA, USA) resulting in a retinal cell suspension.