collagenase type 4  (Worthington Biochemical)


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    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type 4
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type 4/product/Worthington Biochemical
    Average 99 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    collagenase type 4 - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Cyclic AMP-dependent protein kinase and Epac mediate cyclic AMP responses in pancreatic acini
    Article Snippet: .. The pancreas was minced in buffer containing 40 mM Tris (pH 7.4), 95 mM NaCl, 4.7 mM KCl, 0.6 mM MgCl2 , 1.3 mM CaCl2 , 1 mM NaH2 PO4 , 10 mM glucose, 2 mM glutamine, plus 0.1% BSA, 1 × MEM-nones-sential amino acids (GIBCO-BRL, San Jose, CA), and 50 U/ml of type-4 collagenase (Worthington, Freehold, NJ) and then incubated for 1 h at 37°C. .. The digest was filtered through a 300–400 μm mesh (Sefar American, Depew, NY) and distributed in a 24-well Falcon tissue culture plate (Becton Dickinson, Franklin Lakes, NJ).

    Article Title: Effects of the antifungal antibiotic clotrimazole on human cardiac repolarization potassium currents
    Article Snippet: .. After 15 min (5 min at a time in fresh solutions), the chunks were incubated for 50 min in a similar solution containing 150–200 U ml−1 collagenase (CLS II, Worthington Biochemical, Freehold, NJ, U.S.A.), 1.2 U ml−1 protease (type XXIV, Sigma Chemical, St Louis, MO, U.S.A.), and 1 mg ml−1 bovine serum albumin (Sigma). ..

    Article Title: P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP
    Article Snippet: .. Ganglia were desheathed, cut and incubated in 4 ml Ca2+ /Mg2+ -free Hanks' balanced salt solution with 10 m m Hepes buffer (pH 7.0) (HBSS) (Life Technologies) containing 1.5 mg ml−1 collagenase (Class-II; Worthington Biochemical Corporation, Reading, UK) and 6 mg ml−1 bovine serum albumin (Sigma) at 37°C for 40 min. Ganglia were then incubated with 4 ml HBSS containing 1 mg ml−1 trypsin (Sigma) at 37°C for 20 min. ..

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

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  • 99
    Worthington Biochemical collagenase type 4
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