collagenase type 4  (Worthington Biochemical)


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    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type 4
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type 4/product/Worthington Biochemical
    Average 99 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    collagenase type 4 - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Cytometry:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Blocking Assay:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Mouse Assay:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Lysis:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Staining:

    Article Title: A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose
    Article Snippet: .. Mice were then lavaged with sterile PBS with 3 mM EDTA BALF and sera were used to determine antibody titers by ELISA In some experiments, lungs and tracheobronchial lymph nodes (TBLNs) were additionally collected and either homogenized through a wire mesh screen, or digested with agitation in 0.2% collagenase (Worthington Biochemical Corporation; Lakewood, NJ) with DNase (Sigma) at 37 °C for 1 h. Red blood cells were lysed from the lung homogenates using ACK lysis buffer, washed, resuspended in FcR block (clone 93), and stained for flow cytometry. ..

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

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    Worthington Biochemical collagenase type 4
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