collagenase type 2  (Worthington Biochemical)


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  • 99
    Name:
    Collagenase Type 2
    Description:
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    Catalog Number:
    ls004174
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type 2
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
    Average 99 stars, based on 446 article reviews
    Price from $9.99 to $1999.99
    collagenase type 2 - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Exogenous Stimulation of Human Intervertebral Disc Cells in 3-Dimensional Alginate Bead Culture With BMP2 and L51P: Cytocompatibility and Effects on Cell Phenotype
    Article Snippet: .. Briefly, cells were isolated by pronase (Roche, Basel, Switzerland) followed by collagenase type 2 (Worthington, London, UK) enzymatic digestion as previously reported. .. NPCs, AFCs and CEPCs were expanded in proliferation medium (low-glucose [1g/L] Dulbecco’s Modified Eagle Medium supplemented with 10% foetal bovine serum [FBS] and penicillin/streptomycin [P/S]).

    Incubation:

    Article Title: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
    Article Snippet: .. Dermis was minced and then incubated with 2 mg/ml collagenase type 2 (CLS-2; Worthington Biochemical, Lakewood, NJ, USA) in Tyrode’s solution for 60–90 minutes. ..

    FACS:

    Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance
    Article Snippet: .. Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase). .. Stromal pellets were incubated in red blood cell lysis buffer (Sigma Aldrich), washed, and stained with fluorescent-conjugated antibodies for CD45, CD31, Sca1, CD29, CD34, and CD24 as described .

    Mouse Assay:

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart
    Article Snippet: .. Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington). ..

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  • 84
    Worthington Biochemical hausp deficient osteoprogenitors cobs
    <t>HAUSP</t> is required for maturation of <t>osteoprogenitors</t> in vitro and in vivo. a – e COBs isolated from P5 Hausp fl/fl pups were infected with lentiviruses expressing vector (WT) or Cre recombinase ( Hausp KO) and cultured under osteogenic conditions. RT-PCR analysis to measure mRNA levels of Hausp and osteogenic genes ( a ) and ALP activity ( b , left) and staining (b, right) were performed 7 days after the culture. Mineralization was assessed by alizarin red staining 16 days after the culture ( c ). Protein ( d ) and mRNA ( e ) levels of RUNX2 were assessed in these COBs. Scale bar, 100 μm ( b ). a , e n = 4; b , c n = 9. f shCtrl or sh Hausp -expressing C3H10T1/2 cells were transfected with OG2-luc and Renilla in the presence or absence of RUNX2 overexpression. Two days after transfection, OG2-luc activity was measured and normalized to Renilla . ( n = 6 biologically independent samples). Hausp -sufficient or -deficient COBs were infected with lentiviruses expressing vector or RUNX2 , cultured under osteogenic conditions for 7 days, and ALP activity ( g ) and mRNA levels of Bglap2 ( h ) were assessed. g n = 6; h n = 4 biologically independent samples). i Hausp mRNA levels in P0 Hausp fl/fl , Osx-Cre , and Hausp +/Osx calvaria. ( n = 4). j , l , m MicroCT analysis shows 3D-reconstruction of calvaria ( j ) and femurs ( l ) from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Quantification of femoral bone mass is displayed (m). The arrows indicate hypomineralization areas. Scale bars, 2 mm ( j ); 500 μm ( l ). m n = 10 ( Hausp fl/fl ), 5 ( Osx-Cre ) or 10 ( Hausp +/Osx ). k Alizarin red/alcian blue staining of skeletal preparations of clavicles obtained from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Scale bar, 2 mm. n H E-stained longitudinal sections of femurs from 2-month-old Osx-Cre and Hausp +/Osx male mice. Scale bar, 50 μm. Alizarin red/alcian blue staining of skeletal preparations of calvaria (top) and clavicles (bottom) from P10 Osx-Cre , Csnk2b +/Osx , Hausp +/Osx , and Csnk2b +/Osx ; Hausp +/Osx mice ( o ) and Osx-Cre , Runx2 +/Osx , Runx2 +/Osx ; Csnk2b +/Osx , and Runx2 +/Osx ; Hausp +/Osx mice ( p ). Scale bars, 2 mm ( o , p ). Data are representative of three ( a – l , n – p ) independent experiments or are pooled from two experiments ( m ). A two-tailed unpaired Student’s t test for comparing two groups ( a – c , e – i , m ; error bars, SD of biological replicates).
    Hausp Deficient Osteoprogenitors Cobs, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Worthington Biochemical primary osteoprogenitors cob
    <t>HAUSP</t> is required for maturation of <t>osteoprogenitors</t> in vitro and in vivo. a – e COBs isolated from P5 Hausp fl/fl pups were infected with lentiviruses expressing vector (WT) or Cre recombinase ( Hausp KO) and cultured under osteogenic conditions. RT-PCR analysis to measure mRNA levels of Hausp and osteogenic genes ( a ) and ALP activity ( b , left) and staining (b, right) were performed 7 days after the culture. Mineralization was assessed by alizarin red staining 16 days after the culture ( c ). Protein ( d ) and mRNA ( e ) levels of RUNX2 were assessed in these COBs. Scale bar, 100 μm ( b ). a , e n = 4; b , c n = 9. f shCtrl or sh Hausp -expressing C3H10T1/2 cells were transfected with OG2-luc and Renilla in the presence or absence of RUNX2 overexpression. Two days after transfection, OG2-luc activity was measured and normalized to Renilla . ( n = 6 biologically independent samples). Hausp -sufficient or -deficient COBs were infected with lentiviruses expressing vector or RUNX2 , cultured under osteogenic conditions for 7 days, and ALP activity ( g ) and mRNA levels of Bglap2 ( h ) were assessed. g n = 6; h n = 4 biologically independent samples). i Hausp mRNA levels in P0 Hausp fl/fl , Osx-Cre , and Hausp +/Osx calvaria. ( n = 4). j , l , m MicroCT analysis shows 3D-reconstruction of calvaria ( j ) and femurs ( l ) from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Quantification of femoral bone mass is displayed (m). The arrows indicate hypomineralization areas. Scale bars, 2 mm ( j ); 500 μm ( l ). m n = 10 ( Hausp fl/fl ), 5 ( Osx-Cre ) or 10 ( Hausp +/Osx ). k Alizarin red/alcian blue staining of skeletal preparations of clavicles obtained from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Scale bar, 2 mm. n H E-stained longitudinal sections of femurs from 2-month-old Osx-Cre and Hausp +/Osx male mice. Scale bar, 50 μm. Alizarin red/alcian blue staining of skeletal preparations of calvaria (top) and clavicles (bottom) from P10 Osx-Cre , Csnk2b +/Osx , Hausp +/Osx , and Csnk2b +/Osx ; Hausp +/Osx mice ( o ) and Osx-Cre , Runx2 +/Osx , Runx2 +/Osx ; Csnk2b +/Osx , and Runx2 +/Osx ; Hausp +/Osx mice ( p ). Scale bars, 2 mm ( o , p ). Data are representative of three ( a – l , n – p ) independent experiments or are pooled from two experiments ( m ). A two-tailed unpaired Student’s t test for comparing two groups ( a – c , e – i , m ; error bars, SD of biological replicates).
    Primary Osteoprogenitors Cob, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Worthington Biochemical primary calvarial osteoblasts cobs
    <t>HAUSP</t> is required for maturation of <t>osteoprogenitors</t> in vitro and in vivo. a – e COBs isolated from P5 Hausp fl/fl pups were infected with lentiviruses expressing vector (WT) or Cre recombinase ( Hausp KO) and cultured under osteogenic conditions. RT-PCR analysis to measure mRNA levels of Hausp and osteogenic genes ( a ) and ALP activity ( b , left) and staining (b, right) were performed 7 days after the culture. Mineralization was assessed by alizarin red staining 16 days after the culture ( c ). Protein ( d ) and mRNA ( e ) levels of RUNX2 were assessed in these COBs. Scale bar, 100 μm ( b ). a , e n = 4; b , c n = 9. f shCtrl or sh Hausp -expressing C3H10T1/2 cells were transfected with OG2-luc and Renilla in the presence or absence of RUNX2 overexpression. Two days after transfection, OG2-luc activity was measured and normalized to Renilla . ( n = 6 biologically independent samples). Hausp -sufficient or -deficient COBs were infected with lentiviruses expressing vector or RUNX2 , cultured under osteogenic conditions for 7 days, and ALP activity ( g ) and mRNA levels of Bglap2 ( h ) were assessed. g n = 6; h n = 4 biologically independent samples). i Hausp mRNA levels in P0 Hausp fl/fl , Osx-Cre , and Hausp +/Osx calvaria. ( n = 4). j , l , m MicroCT analysis shows 3D-reconstruction of calvaria ( j ) and femurs ( l ) from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Quantification of femoral bone mass is displayed (m). The arrows indicate hypomineralization areas. Scale bars, 2 mm ( j ); 500 μm ( l ). m n = 10 ( Hausp fl/fl ), 5 ( Osx-Cre ) or 10 ( Hausp +/Osx ). k Alizarin red/alcian blue staining of skeletal preparations of clavicles obtained from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Scale bar, 2 mm. n H E-stained longitudinal sections of femurs from 2-month-old Osx-Cre and Hausp +/Osx male mice. Scale bar, 50 μm. Alizarin red/alcian blue staining of skeletal preparations of calvaria (top) and clavicles (bottom) from P10 Osx-Cre , Csnk2b +/Osx , Hausp +/Osx , and Csnk2b +/Osx ; Hausp +/Osx mice ( o ) and Osx-Cre , Runx2 +/Osx , Runx2 +/Osx ; Csnk2b +/Osx , and Runx2 +/Osx ; Hausp +/Osx mice ( p ). Scale bars, 2 mm ( o , p ). Data are representative of three ( a – l , n – p ) independent experiments or are pooled from two experiments ( m ). A two-tailed unpaired Student’s t test for comparing two groups ( a – c , e – i , m ; error bars, SD of biological replicates).
    Primary Calvarial Osteoblasts Cobs, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HAUSP is required for maturation of osteoprogenitors in vitro and in vivo. a – e COBs isolated from P5 Hausp fl/fl pups were infected with lentiviruses expressing vector (WT) or Cre recombinase ( Hausp KO) and cultured under osteogenic conditions. RT-PCR analysis to measure mRNA levels of Hausp and osteogenic genes ( a ) and ALP activity ( b , left) and staining (b, right) were performed 7 days after the culture. Mineralization was assessed by alizarin red staining 16 days after the culture ( c ). Protein ( d ) and mRNA ( e ) levels of RUNX2 were assessed in these COBs. Scale bar, 100 μm ( b ). a , e n = 4; b , c n = 9. f shCtrl or sh Hausp -expressing C3H10T1/2 cells were transfected with OG2-luc and Renilla in the presence or absence of RUNX2 overexpression. Two days after transfection, OG2-luc activity was measured and normalized to Renilla . ( n = 6 biologically independent samples). Hausp -sufficient or -deficient COBs were infected with lentiviruses expressing vector or RUNX2 , cultured under osteogenic conditions for 7 days, and ALP activity ( g ) and mRNA levels of Bglap2 ( h ) were assessed. g n = 6; h n = 4 biologically independent samples). i Hausp mRNA levels in P0 Hausp fl/fl , Osx-Cre , and Hausp +/Osx calvaria. ( n = 4). j , l , m MicroCT analysis shows 3D-reconstruction of calvaria ( j ) and femurs ( l ) from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Quantification of femoral bone mass is displayed (m). The arrows indicate hypomineralization areas. Scale bars, 2 mm ( j ); 500 μm ( l ). m n = 10 ( Hausp fl/fl ), 5 ( Osx-Cre ) or 10 ( Hausp +/Osx ). k Alizarin red/alcian blue staining of skeletal preparations of clavicles obtained from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Scale bar, 2 mm. n H E-stained longitudinal sections of femurs from 2-month-old Osx-Cre and Hausp +/Osx male mice. Scale bar, 50 μm. Alizarin red/alcian blue staining of skeletal preparations of calvaria (top) and clavicles (bottom) from P10 Osx-Cre , Csnk2b +/Osx , Hausp +/Osx , and Csnk2b +/Osx ; Hausp +/Osx mice ( o ) and Osx-Cre , Runx2 +/Osx , Runx2 +/Osx ; Csnk2b +/Osx , and Runx2 +/Osx ; Hausp +/Osx mice ( p ). Scale bars, 2 mm ( o , p ). Data are representative of three ( a – l , n – p ) independent experiments or are pooled from two experiments ( m ). A two-tailed unpaired Student’s t test for comparing two groups ( a – c , e – i , m ; error bars, SD of biological replicates).

    Journal: Nature Communications

    Article Title: A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation

    doi: 10.1038/s41467-020-16038-6

    Figure Lengend Snippet: HAUSP is required for maturation of osteoprogenitors in vitro and in vivo. a – e COBs isolated from P5 Hausp fl/fl pups were infected with lentiviruses expressing vector (WT) or Cre recombinase ( Hausp KO) and cultured under osteogenic conditions. RT-PCR analysis to measure mRNA levels of Hausp and osteogenic genes ( a ) and ALP activity ( b , left) and staining (b, right) were performed 7 days after the culture. Mineralization was assessed by alizarin red staining 16 days after the culture ( c ). Protein ( d ) and mRNA ( e ) levels of RUNX2 were assessed in these COBs. Scale bar, 100 μm ( b ). a , e n = 4; b , c n = 9. f shCtrl or sh Hausp -expressing C3H10T1/2 cells were transfected with OG2-luc and Renilla in the presence or absence of RUNX2 overexpression. Two days after transfection, OG2-luc activity was measured and normalized to Renilla . ( n = 6 biologically independent samples). Hausp -sufficient or -deficient COBs were infected with lentiviruses expressing vector or RUNX2 , cultured under osteogenic conditions for 7 days, and ALP activity ( g ) and mRNA levels of Bglap2 ( h ) were assessed. g n = 6; h n = 4 biologically independent samples). i Hausp mRNA levels in P0 Hausp fl/fl , Osx-Cre , and Hausp +/Osx calvaria. ( n = 4). j , l , m MicroCT analysis shows 3D-reconstruction of calvaria ( j ) and femurs ( l ) from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Quantification of femoral bone mass is displayed (m). The arrows indicate hypomineralization areas. Scale bars, 2 mm ( j ); 500 μm ( l ). m n = 10 ( Hausp fl/fl ), 5 ( Osx-Cre ) or 10 ( Hausp +/Osx ). k Alizarin red/alcian blue staining of skeletal preparations of clavicles obtained from 2-month-old Hausp fl/fl , Osx-Cre and Hausp +/Osx male mice. Scale bar, 2 mm. n H E-stained longitudinal sections of femurs from 2-month-old Osx-Cre and Hausp +/Osx male mice. Scale bar, 50 μm. Alizarin red/alcian blue staining of skeletal preparations of calvaria (top) and clavicles (bottom) from P10 Osx-Cre , Csnk2b +/Osx , Hausp +/Osx , and Csnk2b +/Osx ; Hausp +/Osx mice ( o ) and Osx-Cre , Runx2 +/Osx , Runx2 +/Osx ; Csnk2b +/Osx , and Runx2 +/Osx ; Hausp +/Osx mice ( p ). Scale bars, 2 mm ( o , p ). Data are representative of three ( a – l , n – p ) independent experiments or are pooled from two experiments ( m ). A two-tailed unpaired Student’s t test for comparing two groups ( a – c , e – i , m ; error bars, SD of biological replicates).

    Article Snippet: Primary Csnk2b- or Hausp -deficient osteoprogenitors (COBs) were isolated from calvaria of 5-day-old Csnk2b fl/fl or Hausp fl/fl neonates, respectively, using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml, Roche, 10165859001) and transduced with either lentiviruses expressing EGFP (control) or cre recombinase .

    Techniques: In Vitro, In Vivo, Isolation, Infection, Expressing, Plasmid Preparation, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Staining, Transfection, Over Expression, Mouse Assay, Two Tailed Test