worthington biochemical coa  (Worthington Biochemical)


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    Worthington Biochemical worthington biochemical coa
    Worthington Biochemical Coa, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/worthington biochemical coa/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    worthington biochemical coa - by Bioz Stars, 2021-04
    86/100 stars

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    Activity Assay:

    Article Title: Protease degradable electrospun fibrous hydrogels
    Article Snippet: Degradation studies Isotropic hydrogels (50 μl) were equilibrated at 37°C in 1 ml of TTC for 48 hours in lo-bind tubes and then placed into fresh sterile TTC Buffer, 5nM rhMMP-2, or sterile 1–500 U ml−1 Type II Collagenase (stock activity – 335 U mg−1 , Worthington Biochemical) in TTC buffer (n=3 per condition). .. Units of activity are given by Worthington Biochemical COA and defined as “one unit liberates one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5”. .. Fibrous hydrogels electrospun as thin films and thick mats (1 cm2 area ~ 1 mm thick dry) were equilibrated at 37°C in TTC Buffer for 48 hours in a non-tissue culture treated plate and then refreshed with TTC Buffer, 10nM rhMMP-2 (with 0.1 wt% reagent diluent (R & D systems) to limit rhMMP-2 adsorption to well surfaces), or 5–500 U ml−1 Type II Collagenase in TTC Buffer (n=3,4 per condition).

    Article Title: Protease degradable electrospun fibrous hydrogels
    Article Snippet: .. Degradation studies Isotropic hydrogels (50 μl) were equilibrated at 37°C in 1 ml of TTC for 48 hours in lo-bind tubes and then placed into fresh sterile TTC Buffer, 5nM rhMMP-2, or sterile 1–500 U ml−1 Type II Collagenase (stock activity – 335 U mg−1 , Worthington Biochemical) in TTC buffer (n=3 per condition). .. Units of activity are given by Worthington Biochemical COA and defined as “one unit liberates one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5”.

    Incubation:

    Article Title: Histone Acetyltransferase and Protein Kinase Activities Copurify with a Putative Xenopus RNA Polymerase I Holoenzyme Self-Sufficient for Promoter-Dependent Transcription
    Article Snippet: .. Workman) or 25 μg of total calf thymus histones (Worthington; fraction HLY) was incubated with 5 μl of S100 extract or dialyzed column fractions for 45 min at 37°C in 30- to 50-μl reaction mixtures. .. Buffer conditions were 50 mM Tris-HCl (pH 7.9), 1 mM DTT, 0.1 mM EDTA, 10% glycerol, 10 mM butyric acid, 0.25 μM acetyl coenzyme A (CoA), and 100 nCi of [3 H] acetyl-CoA (26 Ci/mmol; Sigma).

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  • 86
    Worthington Biochemical worthington biochemical coa
    Worthington Biochemical Coa, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/worthington biochemical coa/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    worthington biochemical coa - by Bioz Stars, 2021-04
    86/100 stars
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