sterile ttc buffer  (Worthington Biochemical)


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    Name:
    Collagenase Type 2
    Description:
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    Catalog Number:
    ls004174
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical sterile ttc buffer
    In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in <t>5nM</t> rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or <t>TTC</t> buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    https://www.bioz.com/result/sterile ttc buffer/product/Worthington Biochemical
    Average 88 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    sterile ttc buffer - by Bioz Stars, 2020-10
    88/100 stars

    Images

    1) Product Images from "Protease degradable electrospun fibrous hydrogels"

    Article Title: Protease degradable electrospun fibrous hydrogels

    Journal: Nature communications

    doi: 10.1038/ncomms7639

    In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p
    Figure Legend Snippet: In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p

    Techniques Used: In Vitro, Activity Assay

    2) Product Images from "Protease degradable electrospun fibrous hydrogels"

    Article Title: Protease degradable electrospun fibrous hydrogels

    Journal: Nature communications

    doi: 10.1038/ncomms7639

    Co-electrospun fibrous MePHA hydrogel scaffolds ( a ) Schematic of co-electrospinning setup to form fibrous scaffolds that contain both protease degradable (green) and non-degradable (red) MePHA fibers. ( b,c ) Representative wide-field fluorescent microscopy images of co-electrospun MePHA at day 0 ( b ) and after 10 days incubation at 37°C in 500 U ml −1 Type II collagenase ( c ). Media (collagenase or TTC buffer) was refreshed every two days to maintain enzyme activity. Scale bars: 100 μm, Zoom – 25 μm.
    Figure Legend Snippet: Co-electrospun fibrous MePHA hydrogel scaffolds ( a ) Schematic of co-electrospinning setup to form fibrous scaffolds that contain both protease degradable (green) and non-degradable (red) MePHA fibers. ( b,c ) Representative wide-field fluorescent microscopy images of co-electrospun MePHA at day 0 ( b ) and after 10 days incubation at 37°C in 500 U ml −1 Type II collagenase ( c ). Media (collagenase or TTC buffer) was refreshed every two days to maintain enzyme activity. Scale bars: 100 μm, Zoom – 25 μm.

    Techniques Used: Microscopy, Incubation, Activity Assay

    In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p
    Figure Legend Snippet: In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p

    Techniques Used: In Vitro, Activity Assay

    3) Product Images from "Protease degradable electrospun fibrous hydrogels"

    Article Title: Protease degradable electrospun fibrous hydrogels

    Journal: Nature communications

    doi: 10.1038/ncomms7639

    In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p
    Figure Legend Snippet: In vitro isotropic MePHA hydrogel degradation 50 μl non-fibrous hydrogels of protease degradable ( a ) and non-degradable ( b ) MePHA equilibrated for 48 hours at 37°C retain their cylindrical shape. Scale bar: 5 mm. ( c , d,e,f ) Quantification of HA release from 2 wt% MePHA hydrogels crosslinked with protease degradable ( c,e ) or non-degradable ( d,f ) methacrylated peptides in 5nM rhMMP-2 ( c,d ), varying concentrations of Type II collagenase ( e,f -listed in units of activity per ml) or TTC buffer. Media (rhMMP-2, collagenase, or TTC buffer) was refreshed every two days to maintain enzyme activity and HA release was quantified by monitoring release of a fluorophore covalently bonded to HA. Error bars represent S.D. (n=3,4). *p

    Techniques Used: In Vitro, Activity Assay

    Related Articles

    Isolation:

    Article Title: Exogenous Stimulation of Human Intervertebral Disc Cells in 3-Dimensional Alginate Bead Culture With BMP2 and L51P: Cytocompatibility and Effects on Cell Phenotype
    Article Snippet: .. Briefly, cells were isolated by pronase (Roche, Basel, Switzerland) followed by collagenase type 2 (Worthington, London, UK) enzymatic digestion as previously reported. .. NPCs, AFCs and CEPCs were expanded in proliferation medium (low-glucose [1g/L] Dulbecco’s Modified Eagle Medium supplemented with 10% foetal bovine serum [FBS] and penicillin/streptomycin [P/S]).

    Incubation:

    Article Title: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
    Article Snippet: .. Dermis was minced and then incubated with 2 mg/ml collagenase type 2 (CLS-2; Worthington Biochemical, Lakewood, NJ, USA) in Tyrode’s solution for 60–90 minutes. ..

    FACS:

    Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance
    Article Snippet: .. Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase). .. Stromal pellets were incubated in red blood cell lysis buffer (Sigma Aldrich), washed, and stained with fluorescent-conjugated antibodies for CD45, CD31, Sca1, CD29, CD34, and CD24 as described .

    Mouse Assay:

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart
    Article Snippet: .. Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington). ..

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