clsafa collagenase  (Worthington Biochemical)


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    Name:
    Collagenase Animal Origin Free B
    Description:
    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented
    Catalog Number:
    ls004145
    Price:
    45
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical clsafa collagenase
    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented
    https://www.bioz.com/result/clsafa collagenase/product/Worthington Biochemical
    Average 96 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    clsafa collagenase - by Bioz Stars, 2021-01
    96/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Locally Produced IL-10 Limits Cutaneous Vaccinia Virus Spread
    Article Snippet: .. Flow cytometric analyses Single cell suspensions of ears, cervical lymph nodes and spleens were prepared by collagenase digestion to liberate immune cells (Type I, Worthington Biochemicals) for 1 hr at 37°C and processed into single cell suspensions prior to filtration through 70 μm nylon cell strainers. .. The following antibodies were used to stain the cells: CD45 (clone 30-F11), CD8a (clone 53–6.7), CD8b (clone eBioH35-17.2), CD4 (clone RM4-5), CD11c (clone HL3), CD11b (clone M1/70), CD69 (clone H1.2F3), IL-10 (clone JES5-16E3), IFN-γ (clone XMG1.2), Granzyme B (clone NGZB), T-bet (clone eBio4B10).

    Filtration:

    Article Title: Locally Produced IL-10 Limits Cutaneous Vaccinia Virus Spread
    Article Snippet: .. Flow cytometric analyses Single cell suspensions of ears, cervical lymph nodes and spleens were prepared by collagenase digestion to liberate immune cells (Type I, Worthington Biochemicals) for 1 hr at 37°C and processed into single cell suspensions prior to filtration through 70 μm nylon cell strainers. .. The following antibodies were used to stain the cells: CD45 (clone 30-F11), CD8a (clone 53–6.7), CD8b (clone eBioH35-17.2), CD4 (clone RM4-5), CD11c (clone HL3), CD11b (clone M1/70), CD69 (clone H1.2F3), IL-10 (clone JES5-16E3), IFN-γ (clone XMG1.2), Granzyme B (clone NGZB), T-bet (clone eBio4B10).

    Fluorescence:

    Article Title: CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities
    Article Snippet: .. Fluorescence Activated Cell Sorting Cell suspensions from harvested embryos were made using 0.5% w/v Dispase II (Roche, Switzerland) and 0.1% w/v CLSAFA Collagenase (Worthington, USA) at 37 °C in Hams F12 solution for 5–10 minutes. .. The digested cells were pelleted and resuspended in PBS containing 2% FBS and strained (40 μm mesh; BD Falcon; Becton, Dickinson and Co., Franklin Lakes, NJ) and FACS sorted using the BD Influx Cell Sorter, with separation based on GFP and mCherry fluorophores.

    Cell Culture:

    Article Title: Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine
    Article Snippet: .. We used xenobiotic-free cell culture reagents for culturing cells, as follows: animal origin-free collagenase (Worthington Biochemical Corporation, Lakewood, NJ, USA), CELLstart, EZPassage Tool, Ca/Mg-free HBSS, D/F12, TrypLE, xeno-free B27, N2 supplement, MSCGM-CD, and ProFreeze CDM (Invitrogen/Gibco, Carlsbad, CA, USA), human serum (Innovative Research, Inc., Novi, MI, USA), basic fibroblast growth factor-2 (bFGF-2; Peprotech, Rocky Hill, NJ, USA), TeSR2, which includes high levels of bFGF and transforming growth factor beta (TGF-β; Stem Cell Technologies, Vancouver, BC, Canada), and Nutristem Stemedia (Stemgent, San Diego, CA, USA). .. Nutristem Stemedia consisted of human recombinant insulin, human serum albumin, transferrin, human fibroblast growth factor, and TGF-β.

    Incubation:

    Article Title: Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
    Article Snippet: .. Samples were centrifuged at 1500 rpm for 5 min and the cell/tissue pellet was incubated with 10 mL animal origin-free collagenase (CLSAFA, 267 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 30 mins with gentle agitation at 37 °C. .. Samples were centrifuged at 2000 rpm for 7 min and cell pellets were resuspended and plated at 1000 cells/cm2 in plastic culture flasks using standard culture medium (minimum essential medium (MEM)α; GE Healthcare Life Sciences HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA, USA), 1% l-glutamine, and 1% penicillin/streptomycin (ThermoFisher Scientific, Gibco, Pittsburgh, PA, USA).

    FACS:

    Article Title: CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities
    Article Snippet: .. Fluorescence Activated Cell Sorting Cell suspensions from harvested embryos were made using 0.5% w/v Dispase II (Roche, Switzerland) and 0.1% w/v CLSAFA Collagenase (Worthington, USA) at 37 °C in Hams F12 solution for 5–10 minutes. .. The digested cells were pelleted and resuspended in PBS containing 2% FBS and strained (40 μm mesh; BD Falcon; Becton, Dickinson and Co., Franklin Lakes, NJ) and FACS sorted using the BD Influx Cell Sorter, with separation based on GFP and mCherry fluorophores.

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  • News
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  • Team
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  • 96
    Worthington Biochemical clsafa collagenase
    Clsafa Collagenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clsafa collagenase/product/Worthington Biochemical
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    clsafa collagenase - by Bioz Stars, 2021-01
    96/100 stars
      Buy from Supplier

    94
    Worthington Biochemical clsafa collaganase
    Clsafa Collaganase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clsafa collaganase/product/Worthington Biochemical
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    clsafa collaganase - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

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