collagenase clsafa  (Worthington Biochemical)


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    Name:
    Collagenase Animal Origin Free B
    Description:
    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented
    Catalog Number:
    LS004145
    Price:
    45
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase clsafa
    Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented
    https://www.bioz.com/result/collagenase clsafa/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase clsafa - by Bioz Stars, 2021-04
    95/100 stars

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    other:

    Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles
    Article Snippet: The cell monolayer was washed with medium without FBS and digested in a collagenase digestion mixture containing 0.45% collagenase (Worthington, Lakewood, NJ, USA), 0.12 mol/L NaCl, 0.01 mol/L KCl, 1000 U/mL of penicillin, 1 mg/mL of streptomycin, and 0.05 mol/L Tris buffer (pH 7.6) at 37°C.

    Article Title: Transplanted progenitors generate functional enteric neurons in the postnatal colon
    Article Snippet: P4 gut was dissociated in 0.5% Dispase II (Roche Applied Science) and 0.05% Collagenase CLSAFA (Worthington Biochemical Corp.) at 37°C for 30 minutes, with gentle pipetting.

    Article Title: PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression
    Article Snippet: The heart was then digested with using buffer containing Collagenase II (230 U/mL) (Worthington; Columbus, OH, USA; LS004147) for 12 min. Atria were removed, left and right ventricles were gently teased apart using forceps and triturated until no large tissue remained.

    Article Title: Role of the PRC2-Six1-miR-25 signaling axis in heart failure.
    Article Snippet: The enzymatic digestion was initiated by the addition of collagenase type B (300 U/mL; Worthington) and hyaluronidase (0.1mg/mL; Worthington) to the perfusion solution.

    Article Title: Age-related Carbonylation of Fibro-cartilage Structural Proteins Drives Tissue Degenerative Modification
    Article Snippet: The remaining pieces of IVD were resuspended in 200 μL sterile PBS and further subjected to collagenase treatment using 8 units of bacterial collagenase type II (Worthington Biochemical Corporation, Lakewood, NJ, USA) overnight at 37°C for each sample.

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: Ca2+ -free collagenase solution (2.4 mg/ml collagenase II [Worthington, Cambridge Biosciences, Cambridge, UK] in perfusion buffer) was perfused for 3–4 min, and then low-Ca2+ collagenase solution (40 μmol/l Ca2+ in Ca2+ -free collagenase solution) for 5–10 min.

    Article Title: Inward rectifier potassium conductance regulates membrane potential of canine colonic smooth muscle
    Article Snippet: Ca2+ -free Hanks’ collagenase solution also contained ATP (0.11 mg ml−1 ), collagenase type III (1 mg ml−1 ; Worthington Biochemical Co.), trypsin inhibitor (2 mg ml−1 ; Sigma), protease type XIV (0.1 mg ml−1 ; Sigma) and bovine serum albumin (1 mg ml−1 ; Sigma).

    Incubation:

    Article Title: Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
    Article Snippet: Human ASC isolation and expansion Adipose tissue (~20 g) was mixed with 10 mL phosphate-buffered saline (PBS) and homogenized (gentleMACS dissociator; Miltenyi Biotec, San Diego, CA, USA). .. Samples were centrifuged at 1500 rpm for 5 min and the cell/tissue pellet was incubated with 10 mL animal origin-free collagenase (CLSAFA, 267 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 30 mins with gentle agitation at 37 °C. .. Samples were centrifuged at 2000 rpm for 7 min and cell pellets were resuspended and plated at 1000 cells/cm2 in plastic culture flasks using standard culture medium (minimum essential medium (MEM)α; GE Healthcare Life Sciences HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA, USA), 1% l-glutamine, and 1% penicillin/streptomycin (ThermoFisher Scientific, Gibco, Pittsburgh, PA, USA).

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  • 95
    Worthington Biochemical collagenase clsafa
    Collagenase Clsafa, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase clsafa/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase clsafa - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    95
    Worthington Biochemical clsafa collaganase
    Clsafa Collaganase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clsafa collaganase/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clsafa collaganase - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

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