clsafa collagenase (Worthington Biochemical)

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Name:

Collagenase Animal Origin Free B

Description:

Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented

Catalog Number:

ls004145

Price:

Size:

100 mg

Source:

Clostridium histolyticum

Cas Number:

9001.12.1

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Structured Review

Worthington Biochemical clsafa collagenase
Prepared from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens must be prevented
https://www.bioz.com/result/clsafa collagenase/product/Worthington Biochemical
Average 94 stars, based on 13 article reviews
Price from $9.99 to $1999.99

clsafa collagenase - by Bioz Stars, 2020-08

94/100 stars

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Images

Modification:

Article Title: Mesenchymal Stem Cell Alterations in Bone Marrow Lesions in Patients With Hip Osteoarthritis
Article Snippet: .. Samples prepared for collagenase treatment were minced using a rongeur (Figure E) and then placed in low‐glucose Dulbecco's modified Eagle's medium (DMEM; Life Technologies) with 20% fetal calf serum (FCS) (Sigma) and animal origin–free collagenase (3,000 units/gm bone) (Worthington Biochemical Corporation) for 4 hours at 37°C . .. After completion of the collagenase treatment, a fraction of the cells (average 1.5 × 105 ) was obtained for flow cytometry, and the rest of the cells were frozen in FCS supplemented with 10% dimethylsulfoxide (Sigma) for later use.

Incubation:

Article Title: Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome
Article Snippet: .. Samples were centrifuged at 1500 rpm for 5 min and the cell/tissue pellet was incubated with 10 mL animal origin-free collagenase (CLSAFA, 267 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 30 mins with gentle agitation at 37 °C. .. Samples were centrifuged at 2000 rpm for 7 min and cell pellets were resuspended and plated at 1000 cells/cm2 in plastic culture flasks using standard culture medium (minimum essential medium (MEM)α; GE Healthcare Life Sciences HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Flowery Branch, GA, USA), 1% l -glutamine, and 1% penicillin/streptomycin (ThermoFisher Scientific, Gibco, Pittsburgh, PA, USA).

Recombinant:

Article Title: Periodontal regeneration in swine after cell injection and cell sheet transplantation of human dental pulp stem cells following good manufacturing practice
Article Snippet: .. The xenobiotic-free cell culture reagents included: animal-free origin collagenase (Worthington Biochemical Corporation, Lakewood, NJ, USA), CELLstart, EZPassage Tool, HBSS-Ca/Mg free, D/F12, TrypLE, xeno-free B27, N2 supplement, MSCGM-CD and ProFreeze CDM (Invitrogen/Gibco, Carlsbad, CA, USA), human serum (Innovative Research, Inc., Novi, Michigan, USA), basic fibroblast growth factor-2 (bFGF-2; Peprotech, Rocky Hill, NJ, USA), TeSR2 which includes high levels of bFGF-2 together with transforming growth factor-β (TGF-β; Stem Cell Technologies, Vancouver, BC, Canada), and Nutristem Stemedia (Stemgent, San Diego, USA), which consists of human recombinant insulin, human serum albumin, transferrin, human fibroblast growth factor, and TGF-β. ..

Article Title: Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration
Article Snippet: .. The xenobiotic-free cell culture medium was used to cell culture, which is containing animal free origin collagenase (Worthington Biochemical Corporation, Lakewood, NJ), CELLstart, EZPassage Tool, HBSS-Ca/Mg free, D/F12, TrypLE, xeno-free B27, N2 supplement, MSCGM-CD and ProFreeze CDM (Invitrogen/Gibco, Carlsbad, CA), human serum (Innovative Research, Inc., Novi, Michigan), basic fibroblast growth factor-2 (bFGF-2) (Peprotech, Rocky Hill, NJ), TeSR2 which includes high levels of bFGF-2 together with transforming growth factor-β (TGF-β) (Stem Cell Technologies, Vancouver, BC, Canada), and Nutristem Stemedia (Stemgent, San Diego), which consists of human recombinant insulin, human serum albumin, transferrin, human fibroblast growth factor, and TGF-β. ..

Cell Culture:

Article Title: Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine
Article Snippet: .. We used xenobiotic-free cell culture reagents for culturing cells, as follows: animal origin-free collagenase (Worthington Biochemical Corporation, Lakewood, NJ, USA), CELLstart, EZPassage Tool, Ca/Mg-free HBSS, D/F12, TrypLE, xeno-free B27, N2 supplement, MSCGM-CD, and ProFreeze CDM (Invitrogen/Gibco, Carlsbad, CA, USA), human serum (Innovative Research, Inc., Novi, MI, USA), basic fibroblast growth factor-2 (bFGF-2; Peprotech, Rocky Hill, NJ, USA), TeSR2, which includes high levels of bFGF and transforming growth factor beta (TGF-β; Stem Cell Technologies, Vancouver, BC, Canada), and Nutristem Stemedia (Stemgent, San Diego, CA, USA). .. Nutristem Stemedia consisted of human recombinant insulin, human serum albumin, transferrin, human fibroblast growth factor, and TGF-β.