cls 2 collagenase coger  (Worthington Biochemical)


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    Worthington Biochemical cls 2 collagenase coger
    Cls 2 Collagenase Coger, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cls 2 collagenase coger/product/Worthington Biochemical
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cls 2 collagenase coger - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Neutralization:

    Article Title: Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques
    Article Snippet: In the case of patients undergoing reconstruction with stem cell-enriched samples, stem cell isolation was performed from aliquots of either non-enriched adipose tissue samples (that is, collected before stem cell enrichment procedures) or stem cell-enriched adipose tissue samples. .. Lipoaspirates were enzymatically dissociated using a 0.025% collagenase II solution for 30 minutes at 37°C (Worthington, Lakewood, NJ, USA) and, after neutralization of the enzyme, were centrifuged at 600 × g for 10 minutes and filtered (Merck Millipore, Darmstadt, Germany). .. To determine the cell yield after isolation, cells less than 70 μm in diameter were counted and recovery was expressed as 106 cells per milliliter of lipoaspirate.

    Isolation:

    Article Title: SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis
    Article Snippet: Cells were spun down and resuspended in Neurobasal-A medium (Thermo Fisher Scientific, 10888022) plus B27 (Thermo Fisher Scientific, 17504044) and plated onto poly-lysine (Sigma-Aldrich, 27964-99-4)-coated dishes. .. Primary mouse fibroblasts were isolated from mouse newborn pups using collagenase type II (Worthington, LS004204) and grown in DMEM plus 10% fetal bovine serum. .. For all the western blot and qPCR studies, passage 7 to 10 primary fibroblasts were used.

    Incubation:

    Article Title: Adenosine triphosphate drives head and neck cancer pain through P2X2/3 heterotrimers
    Article Snippet: Briefly, BALB/c mice were euthanized with isoflurane. .. Trigeminal ganglia were removed, transferred into HBSS and enzyme-digested by incubation with papain (Worthington), collagenase type II (CLS2) (Worthington), and dispase type II (MB). .. Dissociated neurons were plated on glass coverslips coated with poly-d-lysine and laminin and maintained for approximately 2 hr at 37°C at 5% CO2/95% air in F12 media (Gibco BRL) supplemented with 10% FBS.

    Article Title: Effect of Diphenyleneiodonium Chloride on Intracellular Reactive Oxygen Species Metabolism with Emphasis on NADPH Oxidase and Mitochondria in Two Therapeutically Relevant Human Cell Types
    Article Snippet: For the isolation of hAMSCs, the membranes were washed twice with cold PBS and then dissected into pieces of about 2 × 3 cm2 each. .. Subsequently, the pieces were transferred to T75 cell culture flasks and incubated in 1-mg/mL collagenase solution (collagenase type I, CLS1, 280 U/mg, Worthington Biochemical Corporation, Lakewood, NJ, USA) at 37 °C and continuous shaking for 2 h. Afterwards, an equal volume of cold PBS was added, and the cell suspension was filtered through 100 and 22-µm cell strainers (Thermo Fisher Scientific, Waltham, MA, USA and Karlsruhe, Germany). .. The suspension was then centrifuged for 9 min at 400× g and 4 °C, and the cell pellet was resuspended in DMEM HG medium supplemented with 10% FCS, 1% L-glutamine, 1% Pen/Strep, 1 ng/mL basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, CT, USA), and 15 mM HEPES.

    Cell Culture:

    Article Title: Effect of Diphenyleneiodonium Chloride on Intracellular Reactive Oxygen Species Metabolism with Emphasis on NADPH Oxidase and Mitochondria in Two Therapeutically Relevant Human Cell Types
    Article Snippet: For the isolation of hAMSCs, the membranes were washed twice with cold PBS and then dissected into pieces of about 2 × 3 cm2 each. .. Subsequently, the pieces were transferred to T75 cell culture flasks and incubated in 1-mg/mL collagenase solution (collagenase type I, CLS1, 280 U/mg, Worthington Biochemical Corporation, Lakewood, NJ, USA) at 37 °C and continuous shaking for 2 h. Afterwards, an equal volume of cold PBS was added, and the cell suspension was filtered through 100 and 22-µm cell strainers (Thermo Fisher Scientific, Waltham, MA, USA and Karlsruhe, Germany). .. The suspension was then centrifuged for 9 min at 400× g and 4 °C, and the cell pellet was resuspended in DMEM HG medium supplemented with 10% FCS, 1% L-glutamine, 1% Pen/Strep, 1 ng/mL basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, CT, USA), and 15 mM HEPES.

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