cls 2 collagenase coger (Worthington Biochemical)

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Collagenase Type 2

Description:

Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder

Catalog Number:

LS004174

Price:

Source:

Clostridium histolyticum

Size:

100 mg

Cas Number:

9001.12.1

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Structured Review

Worthington Biochemical cls 2 collagenase coger
Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
https://www.bioz.com/result/cls 2 collagenase coger/product/Worthington Biochemical
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cls 2 collagenase coger - by Bioz Stars, 2021-04

99/100 stars

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Concentration Assay:

Article Title: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia
Article Snippet: .. Porcine and bovine NP tissues were digested by 0.19% pronase (Roche, Basel, Switzerland) for 1 hour and subsequent collagenase type 2 at a concentration of 32 U/mL in 25 mL of HG-DMEM supplemented with 10% FCS (Worthington, London, UK) overnight (~14 hours) at 37°C and standard culture conditions (5% CO2 and 100% humidity) [ , ]. .. Cell separation using micro sieves and cytometry using FACS Cells isolated from porcine NP tissues were separated with three cell sieves, i.e., cells > 20 μm, 8-20 μm and cells < 8 μm (CellMicroSieves, BioDesign Inc., New York, USA) [ ] with the goal to identify NPC and NC.

Mouse Assay:

Article Title: The kallikrein-kinin system is falling into pieces: bradykinin fragments are biological active peptidesAngiotensin-( - Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): A new agonist of B(
Article Snippet: Cells were maintained with DMEM-F12 cell media supplemented by 10% FBS and 1% of antibiotic/antimycotic solution at 37°C until usage. .. Mice ventricular adult cardiomyocytes were isolated by collagenase type-2 (Worthington), as previously shown ( ) and kept in Tyrode media (1.3 x 10−1 mol.L-1 NaCl; 5.4 x 10−3 mol.L-1 KCl; 2,5 x 10−2 mol.L-1 HEPES; 5 x 10−4 mol.L-1 MgCl2 ; 3.3 x 10−4 mol.L-1 NaH2 PO4 ; 1.8 x 10−3 mol.L-1 mM CaCl2 ; 2.2 x 10−2 mol.L-1 glucose) until usage. .. For NO quantification, cells were starved with Hank’s Balanced Salt Solution (HBSS) for 1 hour and then loaded with DAF-FM diacetate (Thermo-Fisher) at a final concentration of 5 x 10−6 mol.L-1 for 30 minutes.

Article Title: Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation
Article Snippet: .. For lung harvests, mice were perfused through the right ventricle with PBS and the lungs were carefully excised and placed in type 2 collagenase (Worthington Biochemical Corporation) dissolved in HBSS containing Ca2+ and Mg2+ . .. The lung homogenate was then passed through a 70μm nylon strainer, spun down and treated with red blood cell lysis buffer (Sigma-Aldrich) for 5 minutes.

Isolation:

In Vitro:

Article Title: Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering
Article Snippet: .. 2.5 In vitro Enzymatic Degradation of dMEP NanofibersTo explore the degradation behavior of dMEP scaffolds, acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C, and then digested in 100 ug/mL proteinase K in tris-HCl overnight at 60°C. .. A scaffold from each group was used for SEM imaging to visualize changes in fiber morphology and structure after enzymatic degradation, and the remaining were used for the orthohydroxyproline (OHP) assay to quantify remaining collagen [ ].