collagenase  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical collagenase
    Collagenase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-07
    93/100 stars

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    Incubation:

    Article Title: NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis Pathway
    Article Snippet: The embryos were isolated and placed in PBS (Gibco). .. Then unfixed bulla from embryos were dissected and incubated in calcium-magnesium-free PBS (Invitrogen) containing dispase (1 mg/ml; Invitrogen) and collagenase (1 mg/ml; Worthington) to free the cochlear duct from the surrounding condensed mesenchyme for 10–15 min. After that, the enzyme solution was replaced with Hank’s Balanced Salt Solution (HBSS, Life Technologies) and the spiral ganglia, Reissner’s membrane, and the most basal cochlear segment should be removed to obtain a flat cochlear surface preparation. ..

    Article Title: Sphingosine and FTY720 are potent inhibitors of the transient receptor potential melastatin 7 (TRPM7) channels
    Article Snippet: .. Atrial samples were minced and incubated in collagenase (150–200 U·mL−1 CLS II, Worthington Biochemical, Freehold, NJ, USA, 300 U·mg−1 ) in a shaking water bath at 37°C. .. Isolated cells were harvested after each 10 min digestion period.

    Article Title: 4-Nitro-3-phenylphenol has both androgenic and anti-androgenic-like effects in rats
    Article Snippet: .. After that, the testes were dissociated by incubation in M199 medium (Gibco®) containing 0.71 g/l sodium bicarbonate, 2.21 g/l HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 0.1% BSA, and 25 mg/l soybean trypsin inhibitor (STI), pH 7.4, with 0.25 mg/ml of collagenase (Worthington Biochemical, Freehold, NJ, USA) and then horizontal shaking (90 cycles/min) at 34 C for approximately 10–20 min. After dissociation, the seminiferous tubules were removed by filtration through 100 µm nylon mesh. ..

    Filtration:

    Article Title: 4-Nitro-3-phenylphenol has both androgenic and anti-androgenic-like effects in rats
    Article Snippet: .. After that, the testes were dissociated by incubation in M199 medium (Gibco®) containing 0.71 g/l sodium bicarbonate, 2.21 g/l HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 0.1% BSA, and 25 mg/l soybean trypsin inhibitor (STI), pH 7.4, with 0.25 mg/ml of collagenase (Worthington Biochemical, Freehold, NJ, USA) and then horizontal shaking (90 cycles/min) at 34 C for approximately 10–20 min. After dissociation, the seminiferous tubules were removed by filtration through 100 µm nylon mesh. ..

    Modification:

    Article Title: Potential action of androstenedione on the proliferation and apoptosis of stromal endometrial cells
    Article Snippet: Another piece was fixed in 4% buffered formaldehyde for 24 h, embedded in paraffin, and cut into 5 μm thick sections before in situ analysis of apoptosis and immunohistochemistry. .. Briefly, the tissue was cut into small pieces and suspended in Dulbecco's modified Eagle medium (DMEM) (GibcoBRL), collagenase (370 IU/100 mg tissue) (Worthington, Biochemical Corp. Freehold, NJ, USA) and DNAse (14 KU/100 mg tissue) (Sigma) during 1 h at 37°C. .. Epithelial cells, predominantly from glands, were separated from ESC by decantation and the supernatant containing the ESC was filtered, centrifuged and the cell pellet washed in DMEM.

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    Worthington Biochemical collagenase type cls 1
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    Worthington Biochemical cls iii collagenase
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