hartlib (Worthington Biochemical)

Worthington Biochemical is a verified supplier

Worthington Biochemical manufactures this product

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Name:

Collagenase Type 2

Description:

Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder

Catalog Number:

ls004174

Price:

Size:

100 mg

Source:

Clostridium histolyticum

Cas Number:

9001.12.1

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Structured Review

Worthington Biochemical hartlib
Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
https://www.bioz.com/result/hartlib/product/Worthington Biochemical
Average 88 stars, based on 839 article reviews
Price from $9.99 to $1999.99

hartlib - by Bioz Stars, 2020-10

88/100 stars

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Isolation:

Article Title: Exogenous Stimulation of Human Intervertebral Disc Cells in 3-Dimensional Alginate Bead Culture With BMP2 and L51P: Cytocompatibility and Effects on Cell Phenotype
Article Snippet: .. Briefly, cells were isolated by pronase (Roche, Basel, Switzerland) followed by collagenase type 2 (Worthington, London, UK) enzymatic digestion as previously reported. .. NPCs, AFCs and CEPCs were expanded in proliferation medium (low-glucose [1g/L] Dulbecco’s Modified Eagle Medium supplemented with 10% foetal bovine serum [FBS] and penicillin/streptomycin [P/S]).

Article Title: O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity
Article Snippet: .. For brown adipocyte isolation, freshly dissected interscapular BAT was cut into ~2 mm diameter pieces, digested in Krebs-Ringer buffer supplemented with 1% fatty acid free BSA, 0.1% HEPES (1 M, pH 7.3), 0.8 mg/mL Type 2 Collagenase (Worthington, LS004176), and 1.2 mM calcium chloride at 37 °C for 50 min. Digested tissue was then filtered through a 100 μm membrane and centrifuged at 300 g for 3 min. ..

Incubation:

Article Title: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
Article Snippet: .. Dermis was minced and then incubated with 2 mg/ml collagenase type 2 (CLS-2; Worthington Biochemical, Lakewood, NJ, USA) in Tyrode’s solution for 60–90 minutes. ..

FACS:

Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance
Article Snippet: .. Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase). .. Stromal pellets were incubated in red blood cell lysis buffer (Sigma Aldrich), washed, and stained with fluorescent-conjugated antibodies for CD45, CD31, Sca1, CD29, CD34, and CD24 as described .

Mouse Assay:

Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart
Article Snippet: .. Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington). ..