papain  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 728 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
    Article Snippet: .. For this procedure, brains were dissected at room temperature in 1x PBS (K-D Medical), dissociated in papain (Papain Dissociation System, Worthington Biochemical) by incubation in 20 U/mL papain for 20 min then triturated and 0.005% DNAse added. .. The final volume was adjusted to 200 µL in DMEM fluorobrite (Gibco) and immediately counted using a BD FACSCalibur (BD Biosciences), using a custom gate to include single cells in the forward and side scattered area.

    other:

    Article Title: Essential roles of plexin-B3+ oligodendrocyte precursor cells in the pathogenesis of Alzheimer’s disease
    Article Snippet: The hippocampus and other brain regions were dissected according to the Paxinos and Watson atlas ( http://labs.gaidi.ca/rat-brain-atlas/ ) (see Supplementary Table 1), finely minced and digested in papain (26.1 U/ml) (Worthington, Lakewood, NJ) in Hibernate A (Brainbits, Springfield, IL) containing 2% B27 (Thermo Fisher Scientific, Waltham, MA) and 0.5 mM glutamine (Thermo Fisher Scientific)(referred to as Hibernate A/B27) for 30 min at 30°C with shaking at 170 rpm.

    Cell Culture:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

    Microscopy:

    Article Title: Distinct transcriptomic cell types and neural circuits of the subiculum and prosubiculum along the dorsal-ventral axis
    Article Snippet: .. The entire region containing full DV extent of PS-Sub-HA-PrS-PoS-PaS were micro-dissected out with a knife and a microscope, under which the anatomic borders can be identified, and digested with 30 U/ml papain (Worthington #PAP2) in ACSF for 30 mins at 35 °C in a dry oven, with a targeted solution temperature of 30 °C. .. In 1 ml of quenching buffer, the tissue pieces were triturated through a fire-polished pipette, with 600-um diameter opening, approximately 20 times.

    Gel Permeation Chromatography:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

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    • papain based dissociation kit  (Worthington Biochemical)
    99
    Worthington Biochemical papain based dissociation kit
    Papain Based Dissociation Kit, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain based dissociation kit/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain based dissociation kit - by Bioz Stars, 2020-08
    99/100 stars
    		  Buy from Supplier
    papain  (Worthington Biochemical)
    94
    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 728 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2020-08
    94/100 stars
    		  Buy from Supplier

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