papain  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 728 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2020-10
    94/100 stars

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    Isolation:

    Article Title: Staufen 1 amplifies proapoptotic activation of the unfolded protein response
    Article Snippet: .. Cortices from 6 to 7 animals were isolated, cut into 2 mm segments, and incubated with 50 units of papain (Worthington Biochemical, USA) in Earle’s balanced salt solution with 1.0 mM l -cysteine and 0.5 mM EDTA for 15 min at 37 °C. .. Digested tissue was washed with EBSS and mechanical dissociation was performed with a 1 ml micropipette in the presence of 0.1 mg/ml of DNase1 (Sigma-Aldrich).

    Incubation:

    Article Title: Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
    Article Snippet: .. For this procedure, brains were dissected at room temperature in 1x PBS (K-D Medical), dissociated in papain (Papain Dissociation System, Worthington Biochemical) by incubation in 20 U/mL papain for 20 min then triturated and 0.005% DNAse added. .. The final volume was adjusted to 200 µL in DMEM fluorobrite (Gibco) and immediately counted using a BD FACSCalibur (BD Biosciences), using a custom gate to include single cells in the forward and side scattered area.

    Article Title: Staufen 1 amplifies proapoptotic activation of the unfolded protein response
    Article Snippet: .. Cortices from 6 to 7 animals were isolated, cut into 2 mm segments, and incubated with 50 units of papain (Worthington Biochemical, USA) in Earle’s balanced salt solution with 1.0 mM l -cysteine and 0.5 mM EDTA for 15 min at 37 °C. .. Digested tissue was washed with EBSS and mechanical dissociation was performed with a 1 ml micropipette in the presence of 0.1 mg/ml of DNase1 (Sigma-Aldrich).

    Article Title: Single-cell RNA-seq analysis of the brainstem of mutant SOD1 mice reveals perturbed cell types and pathways of amyotrophic lateral sclerosis
    Article Snippet: .. In brief, each tissue was finely dissected and incubated in Hibernate A without calcium (BrainBits, Springfield, IL, USA) supplemented with 12 mg papain (Worthington, Lakewood, NJ, USA). .. This solution is then transferred to a shaker-incubator (set to 30 °C and 60 rpm) for 30 min. After 30 min, using a Pasteur pipette, the solution is triturated three times to release cells from the tissue remnants, and transferred to a new collection tube.

    Gel Permeation Chromatography:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

    Cell Culture:

    Article Title: Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Article Snippet: .. The embryoid bodies were dissociated with papain (Papain dissociation system, Worthington), and the GPC were cultured and expanded in 10 μg/mL poly-L-ornithine (Sigma)/1 μg/mL laminin (Invitrogen)-coated plates in AM supplemented with 20 ng/mL fibroblast growth factor 2 (Joint Protein Central) and 20 ng/mL epidermal growth factor (Peprotech). .. Astrocytes were differentiated from low-confluent GPC cultures in DMEM/F12 Glutamax (Thermo Fisher Scientific) supplemented with N2 and B27 (both from Thermo Fisher Scientific) and 10% fetal bovine serum (FBS, Omega Scientific).

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  • 99
    Worthington Biochemical papain based dissociation kit
    Papain Based Dissociation Kit, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain based dissociation kit/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    papain based dissociation kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Worthington Biochemical papain
    Papain, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/papain/product/Worthington Biochemical
    Average 94 stars, based on 728 article reviews
    Price from $9.99 to $1999.99
    papain - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

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