cellulase  (Worthington Biochemical)


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    Name:
    Cellulase
    Description:
    Chromatographically purified complex containing exo and endoglucanase activities A dialyzed lyophilized powder Tested for lipase protease and nuclease
    Catalog Number:
    LS002598
    Price:
    33
    Source:
    Trichoderma reesei ATCC#26921 (previously T. viride)
    Size:
    250 mg
    Cas Number:
    9012.54.8
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    Structured Review

    Worthington Biochemical cellulase
    Chromatographically purified complex containing exo and endoglucanase activities A dialyzed lyophilized powder Tested for lipase protease and nuclease
    https://www.bioz.com/result/cellulase/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellulase - by Bioz Stars, 2021-07
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Redistribution of Golgi Stacks and Other Organelles during Mitosis and Cytokinesis in Plant Cells 1Redistribution of Golgi Stacks and Other Organelles during Mitosis and Cytokinesis in Plant Cells 1 [w]
    Article Snippet: BY-2 cells 2 to 4 d after subculturing were fixed with 1% (v/v) glutaraldehyde in culture medium for 15 min. After two washes in phosphate-buffered saline (PBS), the cells were transferred to 4 to 7 mL of PBS containing 0.5% (w/v) NaBH4 to reduce autofluorescence of the fixative. .. After this overnight incubation, cells were again washed in PBS and their cell walls were partially digested by treatment with 1% (v/v) cellulase (Worthington, Freehold, NJ) and 1% (v/v) pectinase (Fluka, Buchs, Switzerland) for 30 min. .. Following two additional washes in PBS, the cells were allowed to settle onto a multiwell microscope slide coated with 0.1% (w/v) polyethyleneimine.

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    Article Title: Tlr1612 is the major repressor of cell aggregation in the light-color-dependent c-di-GMP signaling network of Thermosynechococcus vulcanus
    Article Snippet: .. Next, cellulase (12.5 U·mL−1 ; Worthington Biochemical) was added to each cuvette sample, which was then incubated for 30 min at 37 °C to completely disperse the aggregated cells. ..

    Injection:

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    High Performance Liquid Chromatography:

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    Mutagenesis:

    Article Title: A functional cellulose synthase from ascidian epidermis
    Article Snippet: .. In addition, the radioactive product from the CelA mutant carrying the Ciona gene could be digested to water soluble oligosaccharides with β-(1, 4)-endoglucanase-II (EC 3.2.1.4) (0.5 mg of enzyme in 50 mM glycine buffer at pH 3.0 for 4 h at 50°C) or with cellulase (Worthington PB, 0.05 mg/ml enzyme in acetate buffer, pH 5.0, for 2 h at 37°C). .. Hence, the C. savignyi cDNA was at least partially able to complement the celA mutation in vivo and completely able to complement the mutation in vitro , indicating that the C. savignyi gene product does indeed possess cellulose synthase activity. cDNA was cloned into the broad host expression vector pTE3 ( The plasmid carrying the C. savignyi cellulose synthase cDNA behind a lac promoter was introduced into wild-type E. coli K12 strain LE392.

    Expressing:

    Article Title: Structure of the Cellulose Synthase Complex of Gluconacetobacter hansenii at 23.4 Å Resolution
    Article Snippet: The cells were thus cultured to an OD600nm of 1.5 and harvested for protein purification. .. Note that when expressing these proteins, copious amounts (0.2 g/liter) of cellulase (Worthington) had to be added to the culture medium to remove cellulose to facilitate large scale fermentations. .. Detergent Screening for the Purification of AcsAB Complex To achieve efficient solubilization of cellulose synthase TC from the membrane, five different types of detergents were included in the screening.

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  • 94
    Worthington Biochemical cellulase
    Cellulase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellulase/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellulase - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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