cellulase  (Worthington Biochemical)


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    Name:
    Cellulase
    Description:
    Chromatographically purified complex containing exo and endoglucanase activities A dialyzed lyophilized powder Tested for lipase protease and nuclease
    Catalog Number:
    ls002598
    Price:
    33
    Source:
    Trichoderma reesei ATCC#26921 (previously T. viride)
    Size:
    250 mg
    Cas Number:
    9012.54.8
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    Structured Review

    Worthington Biochemical cellulase
    Chromatographically purified complex containing exo and endoglucanase activities A dialyzed lyophilized powder Tested for lipase protease and nuclease
    https://www.bioz.com/result/cellulase/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellulase - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Isolation of a Crystal Matrix Protein Associated with Calcium Oxalate Precipitation in Vacuoles of Specialized Cells 1
    Article Snippet: .. The pellets were then incubated in 1% (v/v) Triton X-100 for 1 h, washed, and incubated for 2 to 3 h at 37°C in a digestion medium containing 2% (w/v) cellulase (Worthington Biochemical, Lakewood, NJ), 1% (w/v) Macerase, 1% (w/v) Pectolyase Y-23, 1% (w/v) BSA, 5 m m CaCl2 , 10 m m MES (pH 5.5), and 2% (w/v) α-amylase (Sigma, St. Louis). ..

    Article Title: Blue/green light-responsive cyanobacteriochromes are cell shade sensors in red-light replete niches
    Article Snippet: .. Next, cellulase (12.5 U•mL−1 ; Worthington Biochemical, NJ, USA) was added to each cuvette sample, which was then incubated for 30 min at 37°C to disperse the aggregated cells completely. .. Next, cellulase (12.5 U•mL−1 ; Worthington Biochemical, NJ, USA) was added to each cuvette sample, which was then incubated for 30 min at 37°C to disperse the aggregated cells completely.

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    Article Title: Cell Wall and Membrane-Associated Exo-?-d-Glucanases from Developing Maize Seedlings 1
    Article Snippet: Two-day-old etiolated maize coleoptiles were isolated, sliced into sections about 1 mm thick, and incubated in Murashige-Skoog salts , pH 6.0, containing 0.25 m sorbitol (protoplast medium). .. The sections were transferred to protoplast medium containing 1% (w/v) cellulase (CELF, Worthington Biochemical, Lakewood, NJ) and 0.2% (w/v) pectinase (PEL, Worthington Biochemical), and incubated with gentle shaking at 30°C. .. The protoplasts were passed through nylon cloth (Nitex, 47-μm2 pores), washed twice with protoplast isolation medium, and, after gentle pelleting, mixed with 20% (w/v) Ficoll in protoplast medium.

    Purification:

    Article Title: Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography
    Article Snippet: The culture was incubated for the desired length of time at 30°C without shaking before plunge freezing. .. For cellulose digestion, 0.2g/L cellulase (Worthington, purified exo- and endo-glucanases, #LS002598) was added. .. Gluconacetobacter xylinus (ATCC 700178/BPR2001) was cultured as described above in Fructose–Peptone–Yeast Extract (FPY) media: 2% fructose, 1% bactopeptone, 0.5% yeast extract and 0.25% K2 HPO4.

    Injection:

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    High Performance Liquid Chromatography:

    Article Title: Initiation, Elongation, and Termination of Bacterial Cellulose Synthesis
    Article Snippet: The reaction mixture was tumbled gently at 60 °C for 12 h. The modified cellulose was isolated by centrifugation and washed with DMSO (2 × 1 mL), water (2 × 1 mL), ethanol (1 × 1 mL), and sterile water (1 × 1 mL). .. The cellulose was treated with 300 μL of 50 mM sodium acetate (pH 5.0) containing 200 U of cellulase (Worthington) and incubated at 37 °C for 24 h. Following digestion, the mixture was passed through a 3 kDa cut-off spin filter and the entire sample was injected onto a C18 reversed-phase HPLC column (Supelco). .. The flow rate was 0.5 mL min–1 with 10 mM sodium acetate (pH 5.0) for 9 mL and then the solvent was changed to 10 mM sodium acetate, pH 5.0/acetonitrile (1/9) for 10 mL.

    Activity Assay:

    Article Title: Reduced Activity of Antioxidant Machinery Is Correlated with Suppression of Totipotency in Plant Protoplasts 1
    Article Snippet: Cellulase Onozuka is rich in xylanase, whereas Macerozyme Onozuka and Cellulase Worthington are free of xylanase activity ( ). .. The macerating enzyme solutions were 1% (w/v) Cellulase R-10, 0.5% (w/v) Macerozyme R-10, or 0.25% (w/v) Cellulase Worthington, 0.5% (w/v) Macerozyme R-10; both exhibited the same cellulase activity. .. The incubation period with cell wall-hydrolyzing enzymes for isolation of T- and NT- tobacco protoplasts was 4 and 16 h, respectively and of grapevine protoplasts it was 4 h ( ).

    Isolation:

    Article Title: Heat Shock Protein HSP101 Binds to the Fed-1 Internal Light Regulator y Element and Mediates Its High Translational Activity
    Article Snippet: .. Protoplasts were isolated from carrot (RCWC) cell suspension by digestion with 0.25% CELF cellulase (Worthington Biochemical Corp., Freehold, NJ), 1% cytolase, 0.05% pectolyase Y23, 0.5% BSA, and 7 mM β-mercaptoethanol in protoplast isolation buffer (12 mM sodium acetate, pH 5.8, 50 mM CaCl2 , and 0.25 M mannitol) for 90 to 120 min. Protoplasts were washed with protoplast isolation buffer followed by electroporation buffer (10 mM Hepes, pH 7.2, 130 mM KCl, 10 mM NaCl, 4 mM CaCl2 , and 0.2 M mannitol), and then they were resuspended in electroporation buffer to ∼106 cells/mL. ..

    Electroporation:

    Article Title: Heat Shock Protein HSP101 Binds to the Fed-1 Internal Light Regulator y Element and Mediates Its High Translational Activity
    Article Snippet: .. Protoplasts were isolated from carrot (RCWC) cell suspension by digestion with 0.25% CELF cellulase (Worthington Biochemical Corp., Freehold, NJ), 1% cytolase, 0.05% pectolyase Y23, 0.5% BSA, and 7 mM β-mercaptoethanol in protoplast isolation buffer (12 mM sodium acetate, pH 5.8, 50 mM CaCl2 , and 0.25 M mannitol) for 90 to 120 min. Protoplasts were washed with protoplast isolation buffer followed by electroporation buffer (10 mM Hepes, pH 7.2, 130 mM KCl, 10 mM NaCl, 4 mM CaCl2 , and 0.2 M mannitol), and then they were resuspended in electroporation buffer to ∼106 cells/mL. ..

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  • 94
    Worthington Biochemical cellulase
    Cellulase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellulase/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellulase - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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