vero cells african green monkey kidney epithelial cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero cells african green monkey kidney epithelial cells
    Vero Cells African Green Monkey Kidney Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero cells african green monkey kidney epithelial cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero cells african green monkey kidney epithelial cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    vero cells african green monkey kidney epithelial cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero cells african green monkey kidney epithelial cells
    Vero Cells African Green Monkey Kidney Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero cells african green monkey kidney epithelial cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero cells african green monkey kidney epithelial cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    stat1  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC stat1
    A <t>STAT1</t> mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.
    Stat1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat1/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stat1 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Case Report: Eosinophilic Esophagitis in a Patient With a Novel STAT1 Gain-of-Function Pathogenic Variant"

    Article Title: Case Report: Eosinophilic Esophagitis in a Patient With a Novel STAT1 Gain-of-Function Pathogenic Variant

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.801832

    A STAT1 mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.
    Figure Legend Snippet: A STAT1 mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.

    Techniques Used: Mutagenesis, Sequencing, Western Blot, Transfection, Real-time Polymerase Chain Reaction

    african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg
    African Green Monkey Kidney Vero Normal Fibroblast Cell Line Atcc Ccl 81 Vhg, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg - by Bioz Stars, 2023-02
    94/100 stars

    Images

    monkey kidney epithelial cells vero  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC monkey kidney epithelial cells vero
    The data represent the percentage of <t>viable</t> <t>Caco-2</t> or <t>Vero</t> cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.
    Monkey Kidney Epithelial Cells Vero, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monkey kidney epithelial cells vero/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monkey kidney epithelial cells vero - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Evaluation of bisphenylthiazoles as a promising class for combating multidrug-resistant fungal infections"

    Article Title: Evaluation of bisphenylthiazoles as a promising class for combating multidrug-resistant fungal infections

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0258465

    The data represent the percentage of viable Caco-2 or Vero cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.
    Figure Legend Snippet: The data represent the percentage of viable Caco-2 or Vero cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.

    Techniques Used: Negative Control, Standard Deviation

    vero stat1 knockout ko cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero stat1 knockout ko cells
    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 knockout ko cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 knockout ko cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.741502

    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Infection, Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    Pearson’s product moment correlation coefficient of IFN pathway-related genes.
    Figure Legend Snippet: Pearson’s product moment correlation coefficient of IFN pathway-related genes.

    Techniques Used:

    vero stat1 knockout cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero stat1 knockout cells
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 knockout cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 knockout cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants"

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    Journal: Journal of Virology

    doi: 10.1128/JVI.01437-21

    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Figure Legend Snippet: Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

    Techniques Used: MTT Assay, Concentration Assay

    SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.
    Figure Legend Snippet: SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

    Techniques Used: Infection, Knock-Out, Inhibition

    Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.
    Figure Legend Snippet: Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.

    Techniques Used: Knock-Out, Infection

    vero ccl81 cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero ccl81 cells
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero ccl81 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero ccl81 cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    vero stat1 knockout ko cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero stat1 knockout ko cells
    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 knockout ko cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 knockout ko cells - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: bioRxiv

    doi: 10.1101/2021.07.07.449660

    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    ccl 81vhg cell lines  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC ccl 81vhg cell lines
    Ccl 81vhg Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccl 81vhg cell lines/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ccl 81vhg cell lines - by Bioz Stars, 2023-02
    94/100 stars

    Images

    vero stat1 ko cell line  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC vero stat1 ko cell line
    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in <t>Vero.STAT1</t> KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 ko cell line/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 ko cell line - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "(E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression"

    Article Title: (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression

    Journal: Viruses

    doi: 10.3390/v13040712

    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Techniques Used: Expressing, Activity Assay, Infection, Transfection, Luciferase, Activation Assay, Quantitative RT-PCR, Western Blot

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC vero cells african green monkey kidney epithelial cells
    Vero Cells African Green Monkey Kidney Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero cells african green monkey kidney epithelial cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero cells african green monkey kidney epithelial cells - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    stat1  (ATCC)
    94
    ATCC stat1
    A <t>STAT1</t> mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.
    Stat1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat1/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stat1 - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg
    A <t>STAT1</t> mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.
    African Green Monkey Kidney Vero Normal Fibroblast Cell Line Atcc Ccl 81 Vhg, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC monkey kidney epithelial cells vero
    The data represent the percentage of <t>viable</t> <t>Caco-2</t> or <t>Vero</t> cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.
    Monkey Kidney Epithelial Cells Vero, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monkey kidney epithelial cells vero/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monkey kidney epithelial cells vero - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC vero stat1 knockout ko cells
    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 knockout ko cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 knockout ko cells - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC vero stat1 knockout cells
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 knockout cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 knockout cells - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC vero ccl81 cells
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero ccl81 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero ccl81 cells - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC ccl 81vhg cell lines
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Ccl 81vhg Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccl 81vhg cell lines/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ccl 81vhg cell lines - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    94
    ATCC vero stat1 ko cell line
    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in <t>Vero.STAT1</t> KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero stat1 ko cell line/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero stat1 ko cell line - by Bioz Stars, 2023-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    A STAT1 mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Case Report: Eosinophilic Esophagitis in a Patient With a Novel STAT1 Gain-of-Function Pathogenic Variant

    doi: 10.3389/fimmu.2022.801832

    Figure Lengend Snippet: A STAT1 mutation identified in our patients causes STAT1 gain-of-function. (A) Pedigree of a three-generational family affected by STAT1 mutation (black). The proband (arrow) suffered from eosinophilic esophagitis and chronic mucocutaneous Candidiasis (CMCC). Her mother had a history of life-long esophagitis and CMCC. The proband’s daughter was affected by CMCC, recurrent acute otitis media, and atopic dermatitis. (B) Confirmatory sequencing of the STAT1 gene in our patients revealed a heterozygous mutation, c.194A>C (p.D65A) in the N-terminal domain. (C) Immunoblotting of STAT1-null U3A cells transfected with either wildtype or D65A STAT1 demonstrated normal total levels of STAT1 in the mutant, with elevated levels of pSTAT1 (Tyr701) following stimulation with either IFN-γ or IFN-α. (D) Quantitative real-time PCR demonstrated increased fold induction of CXCL9 in D65A U3A cells after stimulation with either IFN-γ or IFN-α, with increased fold induction of CXCL10 following stimulation with IFN-γ. ns, not significant.

    Article Snippet: STAT1-deficient U3A cells were obtained from ATCC. pCMV6-STAT1 was from OriGene.

    Techniques: Mutagenesis, Sequencing, Western Blot, Transfection, Real-time Polymerase Chain Reaction

    The data represent the percentage of viable Caco-2 or Vero cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.

    Journal: PLoS ONE

    Article Title: Evaluation of bisphenylthiazoles as a promising class for combating multidrug-resistant fungal infections

    doi: 10.1371/journal.pone.0258465

    Figure Lengend Snippet: The data represent the percentage of viable Caco-2 or Vero cells measured as the average absorbance relative to DMSO after exposure to the tested compounds. Dimethyl sulfoxide (DMSO) was used as a negative control. Error bars represent the standard deviation values.

    Article Snippet: Human colorectal adenocarcinoma epithelial cells (Caco-2) (ATCC HTB-37), and monkey kidney epithelial cells (Vero) (ATCC CCL-81-VHG) were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

    Techniques: Negative Control, Standard Deviation

    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Journal: Frontiers in Immunology

    Article Title: Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    doi: 10.3389/fimmu.2021.741502

    Figure Lengend Snippet: SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Article Snippet: The virus was passaged on Vero.STAT1 knockout (KO) cells (ATCC, CCL-81-VHG) and titer was determined by plaque assay in Vero E6 cells (ATCC, CRL-1586) ( ).

    Techniques: Infection, Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    Pearson’s product moment correlation coefficient of IFN pathway-related genes.

    Journal: Frontiers in Immunology

    Article Title: Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    doi: 10.3389/fimmu.2021.741502

    Figure Lengend Snippet: Pearson’s product moment correlation coefficient of IFN pathway-related genes.

    Article Snippet: The virus was passaged on Vero.STAT1 knockout (KO) cells (ATCC, CCL-81-VHG) and titer was determined by plaque assay in Vero E6 cells (ATCC, CRL-1586) ( ).

    Techniques:

    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

    Journal: Journal of Virology

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    doi: 10.1128/JVI.01437-21

    Figure Lengend Snippet: Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

    Article Snippet: Calu-3 (ATCC HTB-55), Vero E6 (CRL-1586), and Vero-STAT1 knockout cells (CCL-81-VHG) were obtained from ATCC.

    Techniques: MTT Assay, Concentration Assay

    SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

    Journal: Journal of Virology

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    doi: 10.1128/JVI.01437-21

    Figure Lengend Snippet: SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

    Article Snippet: Calu-3 (ATCC HTB-55), Vero E6 (CRL-1586), and Vero-STAT1 knockout cells (CCL-81-VHG) were obtained from ATCC.

    Techniques: Infection, Knock-Out, Inhibition

    Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.

    Journal: Journal of Virology

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    doi: 10.1128/JVI.01437-21

    Figure Lengend Snippet: Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.

    Article Snippet: Calu-3 (ATCC HTB-55), Vero E6 (CRL-1586), and Vero-STAT1 knockout cells (CCL-81-VHG) were obtained from ATCC.

    Techniques: Knock-Out, Infection

    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Journal: Viruses

    Article Title: (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression

    doi: 10.3390/v13040712

    Figure Lengend Snippet: (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Article Snippet: Vero.STAT1 KO cell line, a STAT1 gene knockout Vero cells, was purchased from American Type Culture Collection (ATCC, Manassas, WV, USA; ATCC number CCL-81-VHG TM ).

    Techniques: Expressing, Activity Assay, Infection, Transfection, Luciferase, Activation Assay, Quantitative RT-PCR, Western Blot