Journal: Pharmaceuticals

Article Title: Activity of New Synthetic (2-Chloroethylthio)-1,4-naphthoquinones in Prostate Cancer Cells

doi: 10.3390/ph14100949

Figure Lengend Snippet: Pro-apoptotic activity of the synthesized compounds in 22Rv1, PC-3, LNCaP, and MRC-9 cells. ( A ) Analysis of drug-induced DNA fragmentation using PI staining and flow cytometry. Cells that appeared as sub-G1 population were assumed to be apoptotic and have been quantified with Cell Quest Pro software. ( B ) Analysis of the expression of pro-apoptotic proteins using Western blotting. β-Actin and α-tubulin were used as loading controls. ( C – E ) Analysis of apoptotic cells using annexin-V-FITC/PI double staining and flow cytometry. 22Rv1 cells were pre-treated with the pan-caspase inhibitor z-VAD(OMe)-fmk or vehicle (DMSO) for 1 h prior to treatment with the investigated drugs ( C , D ). PC-3, LNCaP, and MRC-9 cells were treated with the investigated drugs without pre-treatment with z-VAD(OMe)-fmk ( E ). Cells that appeared in the lower right and upper right quadrants were assumed to be apoptotic. The flow cytometry data were quantified with Cell Quest Pro software; double-negative cells (annexin-V – /PI – ) were assumed as alive cells ( D ). Cells treated with 10 μM anisomycin (Aniso) were used as positive control. In all the experiments, the time of drug exposure was 48 h. Statistical significance: * p < 0.05 (Student’s t -Test, section D ; or ANOVA followed by a post hoc Dunnett’s test, section A ). n.s.–non significant ( p > 0.05).

Article Snippet: Human prostate cancer cell lines PC-3, DU145, 22Rv1, and LNCaP, as well as human prostate non-cancer cells RWPE-1 and PNT2, and non-cancer human fibroblast MRC-9 cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Activity Assay, Synthesized, Staining, Flow Cytometry, Software, Expressing, Western Blot, Double Staining, Positive Control

Journal: bioRxiv

Article Title: Highly potent photoinactivation of bacteria using a water soluble, cell permeable, DNA-binding photosensitizer

doi: 10.1101/2021.06.11.448055

Figure Lengend Snippet: A co-culture of MRC-9 lung cells and a standardized suspension of N99 E. coli were incubated with A) Br-DAPI (0.42 μM) and propidium iodide (5 μM), or B) propidium iodide only, treated with 365 nm irradiation (4.5 J cm −2 ). Cells were imaged 30 minutes post-treatment for evidence of cell death which was indicated by the red fluorescence of the propidium iodide cell death fluorescent indicator. (C) The cyan fluorescence from Br-DAPI was selectively observed in bacteria and minimally in mammalian cells. Brightfield images were overlayed with fluorescence of propidium iodide (A, B) or Br-DAPI (C). 16x magnification, scale bar 100 μm. PI imaged using Cy5 filter cube: λ ex = 628 nm, λ em = 692 nm bandpass filter. Br-DAPI imaged using DAPI filter cube: λ ex = 387 nm, λ em = 447 nm bandpass filter.

Article Snippet: MRC-9 (human lung fibroblasts) were purchased from American Type Culture Collection (ATCC) and cultured in Eagle’s Minimum Essential Medium (EMEM) with sodium pyruvate and L-glutamine (ATCC), supplemented with 10% FBS and 1% antibiotic-antimycotic solution.

Techniques: Co-Culture Assay, Incubation, Irradiation, Fluorescence