ccl 211 cell lines  (ATCC)


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    ATCC ccl 211 cell lines
    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, <t>CCL-211)</t> and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    Ccl 211 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 211 cell lines - by Bioz Stars, 2024-04
    92/100 stars

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    1) Product Images from "Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library"

    Article Title: Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-135

    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    Figure Legend Snippet: Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD. "*" denotes p < 0.05 when compared with IC 50 values of CCL-211 cells and "†" denotes p < 0.05 when compared with IC 50 values of WI-38 cells. B. CCL-211 cells were incubated in the absence and presence of 50 μM hematein for 48 hours. Block arrows indicate staining of the nucleus after hematein incubation. Original magnification: × 100.

    Techniques Used: Inhibition, Cell Culture, Cell Viability Assay, Incubation, Blocking Assay, Staining

    ccl 211 cell lines  (ATCC)


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    ATCC ccl 211 cell lines
    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, <t>CCL-211)</t> and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    Ccl 211 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccl 211 cell lines/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ccl 211 cell lines - by Bioz Stars, 2024-04
    92/100 stars

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    1) Product Images from "Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library"

    Article Title: Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-135

    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    Figure Legend Snippet: Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD. "*" denotes p < 0.05 when compared with IC 50 values of CCL-211 cells and "†" denotes p < 0.05 when compared with IC 50 values of WI-38 cells. B. CCL-211 cells were incubated in the absence and presence of 50 μM hematein for 48 hours. Block arrows indicate staining of the nucleus after hematein incubation. Original magnification: × 100.

    Techniques Used: Inhibition, Cell Culture, Cell Viability Assay, Incubation, Blocking Assay, Staining

    hs888lu  (ATCC)


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    ATCC hs888lu
    Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    211 american type culture collection  (ATCC)


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    ATCC 211 american type culture collection
    211 American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human adult lung fibroblast line hs888lu  (ATCC)


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    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    hs888lu  (ATCC)


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    ATCC hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    hs888lu - by Bioz Stars, 2024-04
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    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    hs888lu  (ATCC)


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    ATCC hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hs888lu - by Bioz Stars, 2024-04
    92/100 stars

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    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    human adult lung fibroblast line hs888lu  (ATCC)


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    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human adult lung fibroblast line hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human adult lung fibroblast line hs888lu - by Bioz Stars, 2024-04
    92/100 stars

    Images

    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    cells hs888lu  (ATCC)


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    human lung microvascular endotheial cell line  (ATCC)


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    ATCC ccl 211 cell lines
    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, <t>CCL-211)</t> and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
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    ATCC hs888lu
    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, <t>CCL-211)</t> and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC 211 american type culture collection
    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, <t>CCL-211)</t> and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.
    211 American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC cells hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Cells Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human lung microvascular endotheial cell line
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Lung Microvascular Endotheial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD.

    Journal: BMC Cancer

    Article Title: Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library

    doi: 10.1186/1471-2407-9-135

    Figure Lengend Snippet: Inhibition effects of hematein on cellular growth in normal and cancer cells . A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo ® Luminescent cell viability assay. Data points represent the average of IC 50 value of hematein in triplet experiments and bars indicate SD. "*" denotes p < 0.05 when compared with IC 50 values of CCL-211 cells and "†" denotes p < 0.05 when compared with IC 50 values of WI-38 cells. B. CCL-211 cells were incubated in the absence and presence of 50 μM hematein for 48 hours. Block arrows indicate staining of the nucleus after hematein incubation. Original magnification: × 100.

    Article Snippet: HeLa (CCL-2), HCT116 (CCL-247), A549 (CCL-185), A427 (HTB-53), WI-38 (CCL-75) and CCL-211 cell lines were purchased from American Type Culture Collection (Manassas, VA).

    Techniques: Inhibition, Cell Culture, Cell Viability Assay, Incubation, Blocking Assay, Staining

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Journal: Carcinogenesis

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    doi: 10.1093/carcin/bgx039

    Figure Lengend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Article Snippet: The human lung cancer cell line A549, human fetal lung fibroblast line HFL-1, and human adult lung fibroblast line Hs888Lu were purchased from American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control