cell lines hek293t cell atcc crl 3216 bhk 21 cells atcc ccl  (ATCC)


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    ATCC cell lines hek293t cell atcc crl 3216 bhk 21 cells atcc ccl
    Cell Lines Hek293t Cell Atcc Crl 3216 Bhk 21 Cells Atcc Ccl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bhk 21 ccl 10 cells  (ATCC)


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    ATCC bhk 21 ccl 10 cells
    Bhk 21 Ccl 10 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    baby hamster kidney bhk 21 ccl 10 cell lines  (ATCC)


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    ATCC baby hamster kidney bhk 21 ccl 10 cell lines
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    baby hamster kidney bhk 21 ccl 10 cell lines  (ATCC)


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    ATCC baby hamster kidney bhk 21 ccl 10 cell lines
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    cell lines vero 81 atcc ccl 81 bhk 21 atcc ccl  (ATCC)


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    ATCC cell lines vero 81 atcc ccl 81 bhk 21 atcc ccl
    Cell Lines Vero 81 Atcc Ccl 81 Bhk 21 Atcc Ccl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    baby hamster kidney cells bhk 21 atcc ccl 10 aedes albopictus c6 36 atcc crl 1660 vero e6 atcc crl 1586 h2 35  (ATCC)


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    ATCC baby hamster kidney cells bhk 21 atcc ccl 10 aedes albopictus c6 36 atcc crl 1660 vero e6 atcc crl 1586 h2 35
    Baby Hamster Kidney Cells Bhk 21 Atcc Ccl 10 Aedes Albopictus C6 36 Atcc Crl 1660 Vero E6 Atcc Crl 1586 H2 35, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bhk 21 baby hamster kidney cells  (ATCC)


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    ATCC bhk 21 baby hamster kidney cells
    Bhk 21 Baby Hamster Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bhk 21 cells  (ATCC)


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    ATCC bhk 21 cells
    (A) Schematic of the trans -complementation experiment which involved co-transfecting <t>BHK-21</t> cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).
    Bhk 21 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication"

    Article Title: Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication

    Journal: bioRxiv

    doi: 10.1101/2023.01.26.525530

    (A) Schematic of the trans -complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).
    Figure Legend Snippet: (A) Schematic of the trans -complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Techniques Used: Expressing, Transfection

    (A) Schematic of the trans-complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing S-fragment deletions together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).
    Figure Legend Snippet: (A) Schematic of the trans-complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing S-fragment deletions together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Techniques Used: Expressing, Transfection

    BHK-21 cells were co-transfected with BrU-labelled ptGFP replicon RNA together with 3D HA -labelled replicon RNA. At 4 hours post-transfection cells were fixed and 3D HA -BrU RNA complexes detected by proximity ligation assay (PLA) using anti-HA and anti-BrU primary antibodies together with PLA-labelled secondary antibodies. The in-situ PLA signal is detected as foci in the cell cytoplasm (pseudo-coloured magenta). Cell nuclei were stained with DAPI (pseudo-coloured blue). Images were captured on a Zeiss LSM-880 confocal microscope (bar 10 μm).
    Figure Legend Snippet: BHK-21 cells were co-transfected with BrU-labelled ptGFP replicon RNA together with 3D HA -labelled replicon RNA. At 4 hours post-transfection cells were fixed and 3D HA -BrU RNA complexes detected by proximity ligation assay (PLA) using anti-HA and anti-BrU primary antibodies together with PLA-labelled secondary antibodies. The in-situ PLA signal is detected as foci in the cell cytoplasm (pseudo-coloured magenta). Cell nuclei were stained with DAPI (pseudo-coloured blue). Images were captured on a Zeiss LSM-880 confocal microscope (bar 10 μm).

    Techniques Used: Transfection, Proximity Ligation Assay, In Situ, Staining, Microscopy

    baby hamster kidney strain 21  (ATCC)


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    ATCC baby hamster kidney strain 21
    Baby Hamster Kidney Strain 21, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    baby hamster kidney 21 bhk 21  (ATCC)


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    ATCC baby hamster kidney 21 bhk 21
    Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in <t>BHK-21</t> cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml
    Baby Hamster Kidney 21 Bhk 21, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs"

    Article Title: Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

    Journal: Applied Microbiology and Biotechnology

    doi: 10.1007/s00253-023-12359-w

    Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml
    Figure Legend Snippet: Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml

    Techniques Used: Recombinant, Sequencing, Mutagenesis

    Antigenic characterization of the recombinant viruses. A Bright field and immunofluorescence microscopy images (400 × magnification) of BHK-21 cells infected with either WT or rescued negative marker viruses (WTΔ3A and Y2098FΔ3A) showing detectable fluorescence upon probing with 3B-specific mAb, suggesting virus replication. The fluorescence was absent in the mock-infected control cells. Scale bar: 20 µm. B Western blot analysis of lysate from the WT or recombinant virus-infected BHK-21 cells, probed with rabbit polyclonal serum against deleted 3A region, 3B-specific mAb (10H9D8), rabbit sera raised against 146S of FMDV serotype O, and 3D-specific mAb (6B8D11). Lane 1, mock infected; lane 2, WT; lane 3, WTΔ3A; lane 4, Y2098F; lane 5, Y2098FΔ3A
    Figure Legend Snippet: Antigenic characterization of the recombinant viruses. A Bright field and immunofluorescence microscopy images (400 × magnification) of BHK-21 cells infected with either WT or rescued negative marker viruses (WTΔ3A and Y2098FΔ3A) showing detectable fluorescence upon probing with 3B-specific mAb, suggesting virus replication. The fluorescence was absent in the mock-infected control cells. Scale bar: 20 µm. B Western blot analysis of lysate from the WT or recombinant virus-infected BHK-21 cells, probed with rabbit polyclonal serum against deleted 3A region, 3B-specific mAb (10H9D8), rabbit sera raised against 146S of FMDV serotype O, and 3D-specific mAb (6B8D11). Lane 1, mock infected; lane 2, WT; lane 3, WTΔ3A; lane 4, Y2098F; lane 5, Y2098FΔ3A

    Techniques Used: Recombinant, Immunofluorescence, Microscopy, Infection, Marker, Fluorescence, Western Blot

    Growth kinetics of the recombinant viruses. A Plaque morphology of wild-type (WT), WTΔ3A, and Y2098FΔ3A recombinant viruses in BHK-21 cell monolayers. B Multistep growth kinetic study performed by infecting BHK-21 cells at 0.01 MOI with wild-type (WT), WTΔ3A, and Y2098FΔ3A viruses and titers in tissue culture supernatants determined by TCID 50 analysis. The average titers with standard deviations expressed as Log 10 TCID 50 /ml at indicated time points
    Figure Legend Snippet: Growth kinetics of the recombinant viruses. A Plaque morphology of wild-type (WT), WTΔ3A, and Y2098FΔ3A recombinant viruses in BHK-21 cell monolayers. B Multistep growth kinetic study performed by infecting BHK-21 cells at 0.01 MOI with wild-type (WT), WTΔ3A, and Y2098FΔ3A viruses and titers in tissue culture supernatants determined by TCID 50 analysis. The average titers with standard deviations expressed as Log 10 TCID 50 /ml at indicated time points

    Techniques Used: Recombinant

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    ATCC cell lines hek293t cell atcc crl 3216 bhk 21 cells atcc ccl
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    ATCC bhk 21 cells
    (A) Schematic of the trans -complementation experiment which involved co-transfecting <t>BHK-21</t> cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).
    Bhk 21 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC baby hamster kidney strain 21
    (A) Schematic of the trans -complementation experiment which involved co-transfecting <t>BHK-21</t> cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).
    Baby Hamster Kidney Strain 21, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC baby hamster kidney 21 bhk 21
    Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in <t>BHK-21</t> cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml
    Baby Hamster Kidney 21 Bhk 21, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Schematic of the trans -complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Journal: bioRxiv

    Article Title: Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication

    doi: 10.1101/2023.01.26.525530

    Figure Lengend Snippet: (A) Schematic of the trans -complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing replication-defective 2C or 3B mutations together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Article Snippet: BHK-21 cells obtained from the ATCC (LGC Standard) were maintained in Dulbecco’s modified Eagle’s medium with glutamine (Sigma-Aldrich) supplemented with 10 % FCS, 50 U / mL penicillin and 50 μg / mL streptomycin.

    Techniques: Expressing, Transfection

    (A) Schematic of the trans-complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing S-fragment deletions together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Journal: bioRxiv

    Article Title: Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication

    doi: 10.1101/2023.01.26.525530

    Figure Lengend Snippet: (A) Schematic of the trans-complementation experiment which involved co-transfecting BHK-21 cells with mCherry replicons containing S-fragment deletions together with a WT ptGFP, ptGFP-3B 3 T>K or ptGFP-3D GNN replicon. Fluorescent protein expression was monitored hourly for 24 hours. The data show (B) ptGFP positive cells per well or (C) mCherry positive cells per well at 8 hours post-transfection (n = 2 ± SD).

    Article Snippet: BHK-21 cells obtained from the ATCC (LGC Standard) were maintained in Dulbecco’s modified Eagle’s medium with glutamine (Sigma-Aldrich) supplemented with 10 % FCS, 50 U / mL penicillin and 50 μg / mL streptomycin.

    Techniques: Expressing, Transfection

    BHK-21 cells were co-transfected with BrU-labelled ptGFP replicon RNA together with 3D HA -labelled replicon RNA. At 4 hours post-transfection cells were fixed and 3D HA -BrU RNA complexes detected by proximity ligation assay (PLA) using anti-HA and anti-BrU primary antibodies together with PLA-labelled secondary antibodies. The in-situ PLA signal is detected as foci in the cell cytoplasm (pseudo-coloured magenta). Cell nuclei were stained with DAPI (pseudo-coloured blue). Images were captured on a Zeiss LSM-880 confocal microscope (bar 10 μm).

    Journal: bioRxiv

    Article Title: Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication

    doi: 10.1101/2023.01.26.525530

    Figure Lengend Snippet: BHK-21 cells were co-transfected with BrU-labelled ptGFP replicon RNA together with 3D HA -labelled replicon RNA. At 4 hours post-transfection cells were fixed and 3D HA -BrU RNA complexes detected by proximity ligation assay (PLA) using anti-HA and anti-BrU primary antibodies together with PLA-labelled secondary antibodies. The in-situ PLA signal is detected as foci in the cell cytoplasm (pseudo-coloured magenta). Cell nuclei were stained with DAPI (pseudo-coloured blue). Images were captured on a Zeiss LSM-880 confocal microscope (bar 10 μm).

    Article Snippet: BHK-21 cells obtained from the ATCC (LGC Standard) were maintained in Dulbecco’s modified Eagle’s medium with glutamine (Sigma-Aldrich) supplemented with 10 % FCS, 50 U / mL penicillin and 50 μg / mL streptomycin.

    Techniques: Transfection, Proximity Ligation Assay, In Situ, Staining, Microscopy

    Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml

    Journal: Applied Microbiology and Biotechnology

    Article Title: Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

    doi: 10.1007/s00253-023-12359-w

    Figure Lengend Snippet: Confirmation of the genetic stability of the rescued recombinant viruses and their characterization. A Multiple sequence alignment of the VP2 region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of phenylalanine (F) at 98th position in place of tyrosine (Y) confirms the presence of mutation in Y2098F and Y2098FΔ3A. B Multiple sequence alignment of the 3A non-structural protein region of WT, WTΔ3A, Y2098F, and Y2098FΔ3A after 12 passages in BHK-21 cells. The presence of deletion at 87–144 amino acids in WTΔ3A and Y2098FΔ3A confirms the deletion. C The titers of the rescued viruses—WT, WTΔ3A, and Y2098FΔ3A in the tissue culture supernatants, determined by TCID 50 analysis and mean titer expressed as Log 10 TCID 50 /ml

    Article Snippet: Baby hamster kidney 21 (BHK-21) (clone 13; ATCC, USA, #CCL-10) cell line was maintained in Glasgow Modified Essential Medium (GMEM) (HiMedia, India, #AT1010) supplemented with 10% fetal bovine serum (HiMedia, India, #RM10681) and 60 µg/ml penicillin (Sigma, USA, #P3032), 100 µg/ml streptomycin (Sigma, USA, #S9137), and 100 µg/ml kanamycin (Sigma, USA, #K1377) at 37 °C in 5% CO 2 .

    Techniques: Recombinant, Sequencing, Mutagenesis

    Antigenic characterization of the recombinant viruses. A Bright field and immunofluorescence microscopy images (400 × magnification) of BHK-21 cells infected with either WT or rescued negative marker viruses (WTΔ3A and Y2098FΔ3A) showing detectable fluorescence upon probing with 3B-specific mAb, suggesting virus replication. The fluorescence was absent in the mock-infected control cells. Scale bar: 20 µm. B Western blot analysis of lysate from the WT or recombinant virus-infected BHK-21 cells, probed with rabbit polyclonal serum against deleted 3A region, 3B-specific mAb (10H9D8), rabbit sera raised against 146S of FMDV serotype O, and 3D-specific mAb (6B8D11). Lane 1, mock infected; lane 2, WT; lane 3, WTΔ3A; lane 4, Y2098F; lane 5, Y2098FΔ3A

    Journal: Applied Microbiology and Biotechnology

    Article Title: Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

    doi: 10.1007/s00253-023-12359-w

    Figure Lengend Snippet: Antigenic characterization of the recombinant viruses. A Bright field and immunofluorescence microscopy images (400 × magnification) of BHK-21 cells infected with either WT or rescued negative marker viruses (WTΔ3A and Y2098FΔ3A) showing detectable fluorescence upon probing with 3B-specific mAb, suggesting virus replication. The fluorescence was absent in the mock-infected control cells. Scale bar: 20 µm. B Western blot analysis of lysate from the WT or recombinant virus-infected BHK-21 cells, probed with rabbit polyclonal serum against deleted 3A region, 3B-specific mAb (10H9D8), rabbit sera raised against 146S of FMDV serotype O, and 3D-specific mAb (6B8D11). Lane 1, mock infected; lane 2, WT; lane 3, WTΔ3A; lane 4, Y2098F; lane 5, Y2098FΔ3A

    Article Snippet: Baby hamster kidney 21 (BHK-21) (clone 13; ATCC, USA, #CCL-10) cell line was maintained in Glasgow Modified Essential Medium (GMEM) (HiMedia, India, #AT1010) supplemented with 10% fetal bovine serum (HiMedia, India, #RM10681) and 60 µg/ml penicillin (Sigma, USA, #P3032), 100 µg/ml streptomycin (Sigma, USA, #S9137), and 100 µg/ml kanamycin (Sigma, USA, #K1377) at 37 °C in 5% CO 2 .

    Techniques: Recombinant, Immunofluorescence, Microscopy, Infection, Marker, Fluorescence, Western Blot

    Growth kinetics of the recombinant viruses. A Plaque morphology of wild-type (WT), WTΔ3A, and Y2098FΔ3A recombinant viruses in BHK-21 cell monolayers. B Multistep growth kinetic study performed by infecting BHK-21 cells at 0.01 MOI with wild-type (WT), WTΔ3A, and Y2098FΔ3A viruses and titers in tissue culture supernatants determined by TCID 50 analysis. The average titers with standard deviations expressed as Log 10 TCID 50 /ml at indicated time points

    Journal: Applied Microbiology and Biotechnology

    Article Title: Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

    doi: 10.1007/s00253-023-12359-w

    Figure Lengend Snippet: Growth kinetics of the recombinant viruses. A Plaque morphology of wild-type (WT), WTΔ3A, and Y2098FΔ3A recombinant viruses in BHK-21 cell monolayers. B Multistep growth kinetic study performed by infecting BHK-21 cells at 0.01 MOI with wild-type (WT), WTΔ3A, and Y2098FΔ3A viruses and titers in tissue culture supernatants determined by TCID 50 analysis. The average titers with standard deviations expressed as Log 10 TCID 50 /ml at indicated time points

    Article Snippet: Baby hamster kidney 21 (BHK-21) (clone 13; ATCC, USA, #CCL-10) cell line was maintained in Glasgow Modified Essential Medium (GMEM) (HiMedia, India, #AT1010) supplemented with 10% fetal bovine serum (HiMedia, India, #RM10681) and 60 µg/ml penicillin (Sigma, USA, #P3032), 100 µg/ml streptomycin (Sigma, USA, #S9137), and 100 µg/ml kanamycin (Sigma, USA, #K1377) at 37 °C in 5% CO 2 .

    Techniques: Recombinant